Experimental transmission of encephalopathy and retinopathy induced by nodavirus to sea bream, Sparus aurata L., using different infection models

The transmission of viral encephalopathy and retinopathy (VER) was investigated in juvenile sea bream, Sparus aurata L. Two different infection routes [intraperitoneal (i.p.) and intramuscular injection (i.m.)] were tested at two different temperatures (20 and 26 °C) using sea bream of mean weight 0.7, 2 and 4 g, as well as an immersion challenge performed at 26 °C with sea bream of 0.7 g. Successful transmission of the disease was only achieved by i.m. injection. Mortalities of 100% occurred in sea bream of 0.7 g at day 15 post-infection and 47% in sea bream of both 2 and 4 g at day 30 post-infection in all the experimental infections at 26 °C. No mortalities were ever observed with infections at 20 °C. When mortalities were observed, the virus was detected by immunoperoxidase staining in the SSN-1 cell line inoculated with tissues from infected fish. Histological examination of both normal and infected fish showed a vacuolization in the bipolar and granular layers of the retina of the infected sea bream. This is the first experimentally induced transmission of VER in sea bream. Differences were observed at the time of disease onset depending on water temperature, the route of infection and the age of the juvenile fish.     

斑节对虾Toll9受体基因免疫功能的研究与表达分析

Toll受体蛋白(Toll receptors)是一类重要的模式识别受体,在无脊椎动物先天性免疫系统中发挥重要作用。文章对斑节对虾(Penaeus monodon)新型Toll9受体基因(Pm Toll9)进行了研究:以人源胚胎肾细胞(HEK293T)成功构建体外细胞免疫模型,通过免疫印迹方法证实Pm Toll9重组真核蛋白可在HEK293T中成功表达。双荧光素酶报告系统检测发现在200 ng转染浓度处Pm Toll9对NF-κB报告基因的激活效果显著。q RT-PCR数据证明PmToll9成功激活HEK293T细胞Toll-like receptor(TLR)信号通路,促进通路下游髓样分化因子(My D88)、肿瘤坏死因子(TNF-α)和白介素(IL-10)的上调表达,同时酶联免疫吸附测定结果证明Pm Toll9可促进TNF-α蛋白表达水平显著上调。斑节对虾体内细菌刺激实验结果显示无乳链球菌(Streptococcus agalactiae)可激活Pm Toll9在肝胰腺、肠、淋巴和鳃中的表达,哈维氏弧菌(Vibrio harveyi)可显著抑制Pm Toll9在肝胰腺中的表达。提示无乳链球菌可通过Pm Toll9激活Toll信号通路,引起机体免疫防御反应,而哈维氏弧菌对此过程具有一定的抑制作用。     

Improved performance of gilthead sea bream, Sparus aurata, larvae after ozone disinfection of the eggs

Ozone (O₃) dissolved in seawater (ODS) was evaluated, as an egg disinfectant, on the spawn of captive gilthead sea bream, Sparus aurata, brood stock. Four contact times (CT) were tested (0.6, 1.2, 2.4 and 4.8 mg min L⁻¹) where CT was calculated by multiplying the dissolved O₃ concentration (0.3 mg L⁻¹) by different exposure periods (2, 4, 8, 16 min). There was also a disinfected seawater treatment that contained no O₃ or derived compounds (CT 0) and an untreated seawater control. All ODS treatments reduced egg surface bacterial counts to zero, which was significantly (P<0.05) lower than the CT 0 and the control groups (194 and 1320 plate⁻¹ respectively). Nevertheless, the hatching rate was high in the control and the CT treatments 0, 0.6 and 1.2 (88.7%, 87.3%, 89.5% and 83.7% respectively) while eggs exposed to a CT 2.4 and 4.8 hatched poorly (36.5% and 20.4% respectively), which was likely due, at least in part, to larvae unable to break the egg chorion successfully. Swim‐bladder inflation was significantly higher in the ODS groups (>97%) compared with the control and CT 0 treatments (ca. 70%). The results suggest that a 2‐min exposure of eggs to 0.3 mg O₃ L⁻¹ of ODS (CT 0.6) would improve current protocols in marine larviculture.     

网络视频传输技术在水产病害在线诊断与咨询系统中的应用

随着网络技术的飞速发展,网络的应用越来越广泛。视频传输技术,视频流控制技术,带宽技术等新技术的应用,实现了视频和音频等连续媒体在网络中的传输。本文叙述网络视频传输技术在水产病害在线诊断与咨询系统中的应用。     

无级变速器电子控制单元硬件在环仿真系统

设计了无级变速器电子控制单元硬件在环仿真系统,进行了无级变速器电子控制单元硬件在环仿真试验,并且对仿真测试及优化后的电子控制单元进行了实车试验验证.结果均表明,该系统能够对车辆集成模型与电子控制单元的控制算法进行实时验证及优化控制,为无级变速器电子控制单元开发提供了一个仿真测试平台.     

电激励信号频率对红点病苹果采后电学特性影响

研究了红点病富士苹果采后果实电特性的变化规律,为从电特性角度揭示红点病病害发病的内在机制提供理论参考。运用LCR电子测量仪测定了20℃恒温条件下贮藏70 d的红点病果实在100 Hz、1 kHz、10 kHz、100 kHz和1 MHz频率下复阻抗等电学参数。结果表明,在同一贮藏时间下,随着频率的增加,不论是病果还是正常果其果实的介电常数ε′、复阻抗Z、电抗X和电感L_p值均在减小;果实的Z、X和L_p间存在着显著相关;对100 Hz下以及1、10、100 kHz和1MHz的病果和正常果的Z、X和L_p进行方差分析结果显示,两者差异均不显著(P>0.05),但两者在ε′和损耗因子tanδ上有明显差异(P<0.05)。tanδ和ε′基本可以反映水果的实际品质。     

基于非接触式速度测量的排种驱动系统

介绍了一种利用非接触式速度测量装置驱动排种器的工作原理.该装置用前后两个光电传感器来探测路面的反射光信号,根据两路信号的时间差得到相对速度,并用该测速装置来驱动步进电机,步进电机带动排种器转动,以达到排种和速度同步的目的.此装置可以改善现有播种机靠地轮驱动时由于打滑造成的排种不均匀的问题,使排种更加均匀化.     

农产品检测的漫射近似传输模型

目前,农产品光学无损检测正成为一种新的检测手段.采用漫射近似传输模型对大量光子在样品中的传输进行计算分析,获得农产品样品中光分布规律信息:散射性越强的介质,其光信号同侧检测越明显;同时看到光信号在2mm内急速衰减,而光纤正可以成为合适的探针.这些工作为合理设计无损检测的光探测器提供了理论依据.     

蔬菜脱水试验台传动装置设计

现有的蔬菜脱水设备生产能力较大,直接用于试验时,一次试验用料过多,更换加热方式麻烦,试验过程复杂.针对这些问题,设计了一小型蔬菜脱水试验台.该试验台体积小,一次试验用料少,大大降低了试验成本.对该试验台的传动和输送装置进行了详细的结构设计及计算,同时还对其张紧装置和张紧机构进行了改进设计.改进后的传动及输送装置结构合理、传动平稳且试验方便,为蔬菜脱水设备的进一步研究及其产业化奠定了良好基础.