S-诱抗素对葡萄果实着色、品质和产量的影响

[目的]明确和评价10%S-诱抗素可溶液剂果穗喷雾对葡萄果实着色的效果,以及对品质和产量的影响,为葡萄绿色高效栽培提供理论和技术支撑。[方法] 设立10%S-诱抗素可溶液剂系列浓度(0、200.0、250.0、333.3和500.0 mg/kg),采用田间小区试验,于果实成熟时调查果实外观指标(果粒纵径、果粒横径、裂果率)、着色指数以及产量指标(单穗重、单粒重、实测小区产量等);室内仪器分析测定不同浓度处理的葡萄品质指标(可溶性固形物、可滴定酸等)。[结果]1.于“夏黑”葡萄果实转色初期喷雾使用10%S-诱抗素可溶液剂,试验所有处理对葡萄果实纵径、横径以及纵横径比、裂果率等指标均未见显著差异。2. 10%S-诱抗素可溶液剂系列浓度处理的着色指数显著地高于清水对照 (P<0.05)。3.经10%S-诱抗素可溶液剂处理后,葡萄的可溶性固形物(TSS)含量明显要高于清水对照,其可滴定酸(TA)含量均显著地低于清水对照处理(P＜0.05)。4. “夏黑”葡萄经10%S-诱抗素处理后,各处理间的单株产量无显著差异,与空白对照相比,各处理的平均增产率在0.84%-1.93%之间。[结论]“夏黑”葡萄于果实黑色初期喷雾施用10%S-诱抗素可溶液剂,可显著提高着色指数,促进果实明显着色;与清水对照相比,10%S-诱抗素可溶液剂对葡萄果实纵径、横径以及裂果率等指标无显著影响;10%S-诱抗素可溶液剂可显著提高果实可溶性固形物(TSS)含量,同时能显著降低可滴定酸(TA)含量,明显提升葡萄果实品质;10%S-诱抗素可溶液剂对葡萄的增产作用不甚明显。在葡萄促进着色使用时,建议使用最优浓度范围为200.0-333.3mg/kg,用水量为833.3L/hm2^。     

Contribution of mutation I142M in fusion protein and Q44R in matrix protein of Newcastle disease virus to virulence in ducks

Although verogenic Newcastle disease viruses (NDVs) generally cause subclinical infection in waterfowls such as ducks, NDVs with high virulence in waterfowl have been sporadically reported. We previously reported that the NDV d5a20b strain, which is obtained by serial passaging of the velogenic 9a5b strain in domestic ducks, showed increased virulence in ducks (Hidaka et al., 2021). The d5a20b strain had 11 amino acid substitutions in its P/V, M, F, HN, and L proteins as compared to 9a5b. In the present study, we generated a series of recombinant (r) NDVs with these amino acid substitutions to identify the molecular basis of virulence of NDV in ducks, and evaluated their influences on virulence and in vitro viral properties. Each of the single amino acid substitutions in either the F protein I142M or the M protein Q44R contributed to the enhancement of intracerebral and intranasal pathogenicity in domestic ducks. The cell-cell fusion activity of the virus with F I142M was five times higher than that of the parental r9a5b. The virus with M Q44R rapidly replicated in duck embryo fibroblasts. Additionally, the rM+F+HN strain, which has the same amino acid sequences as d5a20b in M, F, and HN proteins, showed the highest level of virulence and replication efficiency among the generated recombinant viruses, nearly comparable to rd5a20b. These results suggest that multiple factors are involved in the high growth ability of NDV in duck cells, leading to increased virulence in vivo.     

Effects of tris(1,3-dichloro-2-propyl) phosphate on epididymal sperm parameters in adult male rats

Tris(1,3-dichloro-2-propyl) phosphate (TDCIPP) is widely used as a flame retardant and is known to exhibit anti-androgenic effects in vitro and in vivo. To assess the reproductive toxicity potency of TDCIPP, we investigated the effects of 7 days of TDCIPP oral administration on epididymal sperm motion and concentration in adult male Wistar–Imamichi rats. Thirty-five days after the final administration, sperm parameters were evaluated by computer-assisted sperm analysis. Results showed that sperm swimming progression and vigor and sperm concentration in TDCIPP-treated rats were unexpectedly higher than those in control rats. TDCIPP did not significantly affect the percentage of motile sperms or sperm swimming pattern. These results contribute to the understanding of the biological effects of TDCIPP.     

几种杀虫剂对香蕉花蓟马的田间防治试验

选用60 g/L乙基多杀菌素悬浮剂、5%啶虫脒乳油、480 g/L毒死蜱乳油、450 g/L吡虫啉微乳剂、240 g/L螺虫乙酯悬浮剂、1%甲氨基阿维菌素苯甲酸盐乳油和200 g/L氯虫苯甲酰胺悬浮剂等7种杀虫剂对香蕉花蓟马进行了田间防治试验。结果表明: 当采用每间隔两天施药时,7种供试药剂对香蕉花蓟马都具一定的防治效果,其中以240 g/L螺虫乙酯悬浮剂0.25ml/L对该虫的防效最高,为87.92%,其次为60 g/L乙基多杀菌素悬浮剂0.67 ml/L,其防效为86.02%,再次是450 g/L吡虫啉微乳剂0.33 ml/L,防效为72.00%;5%啶虫脒乳油0.67ml/L、200 g/L氯虫苯甲酰胺悬浮剂0.33 ml/L、480 g/L毒死蜱乳油0.5 ml/L、1%甲氨基阿维菌素苯甲酸盐乳油0.33 ml/L对该虫的防效分别为：62.63%、54.48%、51.47%和45.91%,显著低于240 g/L螺虫乙酯悬浮剂和60 g/L乙基多杀菌素悬浮剂。     

柑橘褐斑病菌对不同抗性柑橘品种防御酶活性的影响

以柑橘褐斑病高抗品种Lw8,中抗品种L2,高感品种Lw14为实验材料,通过孢子喷雾法使柑橘褐斑病菌感染柑橘叶片,研究接菌0d、1d、2d、3d、6d、8d、10d时,柑橘叶片中超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)、苯丙氨酸解氨酶(PAL)、多酚氧化酶(PPO)、几丁质酶(CHT)酶和β-1,3葡聚糖酶(GLU)含量及活性变化规律与品种抗病性的关系。结果表明,接种前,两个抗病品种Lw8、L2中的POD、SOD、PAL、PPO 、GLU、CHT活性显著高于高感品种Lw14,CAT活性则与高感品种Lw14相近;接种后,3个不同抗性品种中SOD、POD、CAT、PAL、PPO 、GLU、CHT 7种防御酶活性较对照明显提高,但不同品种增加幅度不一致,除CAT外,其余6种防御酶活性均表现为抗病品种Lw8、L2增幅显著高于高感品种Lw14,且两个抗病品种一般在接菌3d内6种防御酶活性迅速升高并达到峰值;而高感品种Lw14防御酶(除CAT外)活性或增幅较小或较两个抗病品种滞后。本研究初步探讨了不同抗性品种接种褐斑病菌后7种防御酶的活性动态变化与柑橘品种抗病性关系,为进一步研究其抗病机理奠定基础。     

油菜黑胫病菌和茎基溃疡病菌的LAMP检测方法的建立

油菜黑胫病和油菜茎基溃疡病分别由子囊菌Leptosphaeria biglobosa和L.maculans引起.我国油菜产区仅发现L.biglobosa,未发现L.maculans.因而,L.maculans是我国的对外检疫性对象.这两种真菌形态相似,引起的病害症状相似,给田间快速准确鉴定带来难度.本研究基于环介导等温...     

油茶幼林结实与果枝特性研究

通过对油茶自花授粉全同胞子代、无性系间控制授粉全同胞子代、半同胞子代和无性系四种不同群体的结果枝特性和开花结实特点等主要经济性状的研究,揭示了四种群体及其优良林分的结实特性和产量规律,为油茶杂交组合、优良家系和无性系等良种的生产应用和栽培技术、以及进一步开展杂交和选择育种研究积累了必要的技术和理论知识。     

A 13CO2 Enrichment Experiment to Study the Synthesis Pathways of Polyunsaturated Fatty Acids of the Haptophyte Tisochrysis lutea

The production of polyunsaturated fatty acids (PUFA) in Tisochrysis lutea was studied using the gradual incorporation of a ¹³C-enriched isotopic marker, ¹³CO₂, for 24 h during the exponential growth of the algae. The ¹³C enrichment of eleven fatty acids was followed to understand the synthetic pathways the most likely to form the essential polyunsaturated fatty acids 20:5n-3 (EPA) and 22:6n-3 (DHA) in T. lutea. The fatty acids 16:0, 18:1n-9 + 18:3n-3, 18:2n-6, and 22:5n-6 were the most enriched in ¹³C. On the contrary, 18:4n-3 and 18:5n-3 were the least enriched in ¹³C after long chain polyunsaturated fatty acids such as 20:5n-3 or 22:5n-3. The algae appeared to use different routes in parallel to form its polyunsaturated fatty acids. The use of the PKS pathway was hypothesized for polyunsaturated fatty acids with n-6 configuration (such as 22:5n-6) but might also exist for n-3 PUFA (especially 20:5n-3). With regard to the conventional n-3 PUFA pathway, Δ6 desaturation of 18:3n-3 appeared to be the most limiting step for T. lutea, “stopping” at the synthesis of 18:4n-3 and 18:5n-3. These two fatty acids were hypothesized to not undergo any further reaction of elongation and desaturation after being formed and were therefore considered “end-products”. To circumvent this limiting synthetic route, Tisochrysis lutea seemed to have developed an alternative route via Δ8 desaturation to produce longer chain fatty acids such as 20:5n-3 and 22:5n-3. 22:6n-3 presented a lower enrichment and appeared to be produced by a combination of different pathways: the conventional n-3 PUFA pathway by desaturation of 22:5n-3, the alternative route of ω-3 desaturase using 22:5n-6 as precursor, and possibly the PKS pathway. In this study, PKS synthesis looked particularly effective for producing long chain polyunsaturated fatty acids. The rate of enrichment of these compounds hypothetically synthesized by PKS is remarkably fast, making undetectable the ¹³C incorporation into their precursors. Finally, we identified a protein cluster gathering PKS sequences of proteins that are hypothesized allowing n-3 PUFA synthesis.