沙棘嫩枝微扦插育苗产业化技术的研究——微扦插苗不定根发生的条件研究

试验结果表明,2叶2芽的微扦插枝条,生根率可达90％以上,甚至100％。可直接应用于生产,具有生产使用价值。外源激素ABT1和GGR6,都能显著增加不定根发生率,比对照高出30％以上,GGR6更优于ABT1。沙培作为基质,大大优于水培和土培,因为,沙培有效地调节了不定根发生过程中,水、气、温的矛盾。同一外植体插穗,从上...     

沙棘维生素P粉中铅含量测定的不确定度评估

随机选取青海省生产的沙棘维生素P粉样品,用原子吸收分光光度计石墨炉法测定样品中铅含量,参考《化学分析中测量不确定度的评估指南》的方法,分析铅含量测定过程中的测量不确定度的主要来源,包括溶液定容体积、称量、样品溶液铅浓度测定和测量重复性,计算合成不确定度和扩展不确定度。在该样品铅含量测定中,沙棘维生素P粉样品中铅含量为0.96mg/kg,其扩展不确定度为0.11mg/kg(置信度95%,包含因子K=2)。     

促孕散对持久黄体和卵巢静止奶牛血清生殖激素的影响

【目的】研究促孕散对持久黄体和卵巢静止奶牛雌二醇（E2）、促卵泡素（FSH）、促黄体素（LH）和孕酮（P4）4种生殖激素的影响,探讨促孕散治疗奶牛持久黄体和卵巢静止的作用机理。【方法】用促孕散治疗奶牛持久黄体和卵巢静止,统计发情率,用酶联免疫吸附法（ELISA）检测奶牛血清中的E2、FSH、LH和P4 4种生殖激素,并与正常奶牛对比。【结果】促孕散治疗持久黄体牛发情有效率为91.67%,治疗卵巢静止牛发情有效率为88.89%。促孕散处理前,持久黄体P4水平极显著高于正常组（P＜0.01）,卵巢静止P4极显著低于正常组（P＜0.01）,而两种病型在处理前E2、FSH、LH水平与正常组差异不显著（P＞0.05）。持久黄体患牛在促孕散处理后血清中E2和FSH水平不断升高,与正常组比较差异不显著（P＞0.05）,LH水平在促孕散处理后也逐渐上升,与正常组同时达到峰值,其变化规律与正常组相似,P4水平逐渐降低,与正常组水平差异不显著（P＞0.05）。卵巢静止组在促孕散处理后血清中E2、FSH和LH水平均升高,其变化规律与正常组相似,P4水平出现先升高后降低的变化,变化规律与正常组相似,差异不显著（P＞0.05）。【结论】促孕散对持久黄体和卵巢静止奶牛血清生殖激素具有显著调节和诱导发情的作用。     

Note: Field evaluation ofIsaria fumosorosea in controlling the diamondback moth (Plutella xylostella) in Chinese kale

Twelve entomopathogenic fungi were screened for controlling diamondback moth (Plutella xylostella L.) eggs and larvae in the laboratory. None had any significant ovicidal effect.Isaria fumosorosea CKPF-095 showed the best efficacy in controlling the 2 ^nd instar larvae. It was formulated into a wettable powder at a concentration of 1 × 10⁹ conidia g⁻¹ and used in two field tests. Both tests revealed thatI. fumosorosea CKPF-095 at 1.25 × 10¹³ ha⁻¹ showed as good efficacy as Dipel at 1 × 10¹¹ IU ha⁻¹. http://www.phytoparasitica.org posting June 1, 2008.     

酶法制备鮟鱇鱼骨超微钙粉的研究

为有效开发利用(鮟)(鱇)鱼骨下脚料,实验采用酶解和超微粉碎技术从(鮟)(鱇)鱼骨中制备鱼骨超微钙粉,并对(鮟)(鱇)鱼骨超微钙粉进行生物利用率研究.结果显示,采用碱性蛋白酶净化鱼骨的优化条件为酶解温度50℃,酶解时间2.5 h,pH 11.0,用酶量1 300 U/g,此时的净化脱除率为33.8％;经100 min粉碎后的超微钙粉粒度分布较窄,平均粒径达到最小,为626.9 nm;动物实验结果表明,(鮟)(鱇)鱼骨超微钙粉的生物利用率较高,经100 min粉碎后的超微钙粉组的钙吸收率为79.3％～ 83.5％,明显高于对照的碳酸钙组.最优条件下制备的(鮟)(鱇)鱼骨超微钙粉色泽洁白,颗粒均匀细腻,无异味,钙含量达29.5％.研究表明,(鮟)(鱇)鱼骨超微钙粉是一种优质、安全、利用率极高的钙补充制剂.     

Effect of dietary selenium level on growth performance,body composition and hepatic glutathione peroxidase activities of largemouth bass Micropterus salmoide

An 8‐week growth trial was conducted to determine the effect of dietary selenium (Se) level on growth performance, body composition and hepatic glutathione peroxidase (GPx) activities of largemouth bass. Sodium selenite was added to the fish meal basal diet at 0, 0.2, 0.4, 0.6, 0.8 and 1.0 mg kg^?1 Se providing 0.97, 1.17, 1.42, 1.60, 1.85 and 2.06 mg Se kg^?1 diet respectively. Each diet was fed to triplicate groups of fish (initial mean body weight: 4.95 ± 0.03 g) in a closed indoor recirculating system. The Se concentration in the rearing water was not detectable during the whole experimental period. The highest weight gain was obtained in fish fed diets with 1.60 mg Se kg^?1, which was significant higher (P < 0.05) than the basal diet with 0.97 mg Se kg^?1 and did not differ significantly (P > 0.05) with the other treatments. Feed conversion ratio, protein efficiency ratio, protein productive value, apparent digestibility coefficients of dry matter and muscle composition were not significantly impacted (P > 0.05) by dietary treatments. Fish fed diets with ≥1.42 mg Se kg^?1 obtained higher liver lipid contents than treatments with lower dietary Se levels. Hepatic malondialdehyde (MDA) was unchanged (P > 0.05) in relation to dietary Se concentration. Hepatic GPx and glutathione reductase (GR) activities markedly increased and decreased (P < 0.05) with increasing dietary Se concentration, respectively, and both reached a plateau with ≥1.85 mg Se kg^?1. Based on growth performance, hepatic MDA and enzymatic responses of GPx and GR, the highest Se concentration (2.06 mg kg^?1) employed in our study was not harmful for largemouth bass, and the optimal dietary level should be 1.60–1.85 mg Se kg^?1 from sodium selenite, at a dietary vitamin E level of 400 IU kg^?1.     

不同硒肥施入量对黑花生的产量和硒含量的影响

采用土壤拌施亚硒酸钠的方法,研究不同硒肥施入量对黑花生的产量和硒元素含量的影响.结果表明:随着土壤施硒量的增加,黑花生的产量先增加后减少,且黑花生的硒含量呈递增趋势.当土壤施硒量为2 mg/kg时,黑花生的硒含量达到人类食用的最高标准0.3 mg/kg.当土壤施硒量为1.9mg/kg时,黑花生的产量达到最高,为4 35...     

铁胁迫下硒对水稻养分吸收的影响

通过水培法,在铁胁迫下,研究硒对寒地水稻养分吸收和干物质积累的影响.结果表明,与正常培养液培养( Fe0+Se0)相比,加铁胁迫(Fe+Se0)显著提高了植株体内铁的含量,物质积累降低并不显著,但是抑制了水稻对氮、磷和锰的吸收,氮、磷和锰的积累量分别降低了7.8%、26.7%和74.0%,均达到显著水平(P＜0.05)...     

灵芝孢子粉药理作用研究进展

综述了灵芝孢子粉的药理作用及其研究进展,以全面了解灵芝孢子粉的功效和作用机理,为其深入研究和应用提供参考。     

高Se石煤粉对天麻中Se·Zn·Pb·Cd含量的影响

研究了在天麻生长基质中添加高Se石煤粉对天麻中Se富集作用的影响,以及对天麻吸收Zn、Cd、Pb等元素的影响。结果表明:在添加高Se石煤粉0～20g/kg浓度范围内,天麻能有效地富集Se;并且可促进天麻对微量元素Zn的富集,还能拮抗污染元素Cd和Pb的积累。