MB22木聚糖酶发酵条件的研究

通过因素轮换试验和正交试验,对MB22菌产木聚糖酶的培养基配方和发酵条件进行了研究。结果显示最佳培养基组成是:以玉米芯粉和麸皮为碳源,麸皮120g/l,玉米芯280g/l,(NH4)2SO44g/l,CaCO31.5g/l,Tween80,2.0g/l,MgSO4·7H2O1.5g/l;最佳发酵条件为:起始pH5.0,摇床培养温度30℃,转速160r/min,振荡培养84h。在最佳发酵条件下,MB22菌的最高产酶活力可达898.23U/ml。虽然与一些高产菌株相比该菌株的产酶能力还有待于进一步提高,但该试验结果为进一步进行微生物生产木聚糖酶的研究提供了理论依据。     

浙江地区不同品种紫花苜蓿不同刈割期二茬性状表现

对不同秋眠级别的紫花苜蓿Medicago sativa品种进行适应性试验,研究不同品种不同刈割期株高、分蘖数、根系、茎叶比及生物学产量间的变化,为生产推广提供指导.     

Short‐term releases of CO2 from newly mixed biochar and calcareous soil

The work aimed to quantify native organic C mobilized in one calcareous soil in the 21 days after addition of biochar at a range of large to very large applications. The experiment was carried out in unplanted microcosms, and CO₂ flux was used as a measure of net mineralization. A rapid methodological approach, which does not require ¹³C as a tracer, was used to assess any priming effects induced by the biochar. The amount of CO₂‐C mobilized was small relative to the amount of biochar C and proportional to the amount of the biochar added. The additional CO₂‐C was similar to the content of the water‐soluble organic carbon in the biochar added with each application. No interaction with native soil C, that is priming effect, was observed.     

福建省将乐县金溪建电站前后雨水情变化分析

本文通过对将乐县金溪建电站前的1953～1978年、建电站后的1990～2009年雨水情进行分析,结果表明:自然状态下的将乐县(1953～1978年)无论雨水大小,每年都有洪水超过警戒水位,年最大洪水发生时间在4～7月;1953～1978年有16年的当年最大洪峰水位发生在当年最大日降雨量当日至第16日内,频率为61.5...     

互联网订单模式与食用菌产业融合的可行性发展

系统阐述了互联网订单生产模式在我国食用菌产业中实施的可行性。首先从我国目前食用菌产业的低成本生产现状分析了订单生产的战略可行性;其次从食用菌产业的国际环境和互联网科技的发展方面分析了发展订单式生产的有利因素,提出了创新食用菌产业订单模式的一些建议。指出了巩固订单模式实践效果应注意的若干问题。对促进我国食用菌产业创新发展模式提供了借鉴。     

Associations between postpartum diseases and milk yield and changes in body condition between drying off and parturition of dairy cows in Argentina

ABSTRACT

Aims: To assess the change in body condition score (BCS) during the early and late dry periods and its association with postpartum diseases and milk yield in grazing dairy cows from central Argentina.

Methods: BCS assessments during the dry period, and cow health and milk production records up to 90 days in milk (DIM), were collated for cows from 28 farms at monthly visits between 2007 and 2008. Cows were categorised into four groups; those in Group 1 (n=7,067) maintained or gained BCS during the early and late dry periods; Group 2 (n=2,615) maintained or gained BCS during the early dry period and lost BCS during the late dry period; Group 3 (n=1,989) lost BCS during the early dry period and maintained or gained BCS during the late dry period; and Group 4 (n=5,144) lost BCS during the early and late dry periods.

Results: Cows in Group 1 had reduced odds of having retained fetal membranes (RFM), metritis, and clinical mastitis up to 90 DIM than cows in Group 2 (p<0.001), but the odds of disease were similar to cows in Group 3. The odds of having RFM or clinical mastitis tended to be lower in cows in Group 1 than cows in Group 4 (p=0.08). The odds of cows being culled or dying during the first 90 DIM were lower for cows in Group 1 than for those in Groups 2, 3, and 4 (p≤0.05). Mean accumulated milk yield up to 90 DIM was higher in cows in Group 1 than Group 2 and Group 4 (p<0.001), but was similar to that of cows in Group 3 (p=0.28).

Conclusions and clinical relevance: Cows that lost BCS during the late dry period had increased odds of being diagnosed with several postpartum diseases and had decreased milk yield compared to cows that maintained or gained BCS during the entire dry period. Loss of BCS during any stage of the dry period was also associated with increased incidence of culling or death during the first 90 DIM. These results should raise awareness among dairy cattle producers of the importance of properly managing cow body condition during the dry period, especially during the late dry period.     

Atmospheric humidity and genotype are key determinants of the diurnal stomatal conductance pattern

Great understanding of the genotypic difference in diurnal stomatal conductance (g_s) pattern and the key determinants of the pattern is important for saving water by adopting cultivars appropriately. Fifteen wheat genotypes were studied under different soil conditions and various meteorological conditions with pot cultivation in rain shelter for two years. Genotype and air humidity were found to be key determinants of diurnal g_s pattern. All genotypes under low relative humidity (LRH) and most genotypes under high relative humidity (HRH) displayed a gradual decline pattern from morning through the afternoon. Under moderate relative humidity (MRH), all genotypes present a single-peak curve pattern, but they differed in peak time, which may lead to unreliable g_s comparison between genotypes and get ostensible contrasting materials. The stomatal conductance was significantly different among genotypes under LRH and the increased g_s magnitude is also significantly different when it was compared between LRH and HRH. The present results provide new thinking for selecting and adopting appropriate cultivars with specific stomata traits for the area with various meteorological conditions.     

MacCormack算法在长输管道瞬变流动仿真中的应用

为了提高长输管道仿真精度和速率,基于MacCormack格式基本原理和流动方程建立了长输液体管道水力瞬变流动仿真模型。以某管道为例,分别采用该仿真算法和特征线法模拟了阀门关闭和流量增加引起的瞬变流动,并探讨了MacCormack格式中不同边界条件处理方法及Courant常数值对模拟结果的影响。结果表明:MacCormack格式可用于精确仿真长输管道瞬变流动过程,采用特征线法求解预估层边界参数后,其振荡幅度、收敛速度均优于特征线仿真方法;同时,采用特征线法处理MacCormack格式边界条件较线性外插法更易收敛。研究成果可为长输管道瞬变流动仿真提供参考。     

秸秆覆盖与镇压对小黑麦生长状况的影响

以小黑麦作为研究材料,通过设置5种不同的秸秆覆盖与镇压处理,在小黑麦的各个生长期对其进行生理指标的测定,研究秸秆覆盖与镇压对小黑麦鲜重和干物质重、株高、次生根、叶片数的影响.试验结果表明:5种处理下小黑麦的生长状况呈现出了:A4＞ A3＞ A＞ A1＞ A5.而且A4处理下小黑麦的生长状况明显大于其他各组处理下小黑麦的...     

干旱矿区不同干扰强度下土壤种子库特征

土壤种子库是矿区植被恢复的重要因素,其特征在一定程度上反映了区域的植被恢复潜力。本研究通过样地调查和室内萌发法对乌海新星煤矿区3种不同干扰条件下的土壤种子库及与其地表植被之间的关系进行分析。结果表明:1)新星矿区土壤种子库共有18种植物,隶属于7科16属,物种生活型以多年生草本植物为主;2)新星矿区土壤种子库平均储量较小(226.51~739.17粒·m^?2),并且随着土层深度增加而呈递减趋势,其90%以上的种子储藏在0?10 cm的表层土壤;3)研究区土壤种子库植物与地上植被的共有物种数少,土壤种子库与地上植被的植物群落相似性较低;4)随着干扰强度的增大,种子库的植被恢复潜力呈现先增大后减少的趋势:道路周边受到中度干扰的自然植被区朝着有利的方向发展,而采矿周边受重度干扰的自然植被区会受到较大负面影响,其多样性以及与其他分区植物群落的相似性较小,种子密度显著降低(P<0.05)。