吹扫捕集-气相色谱质谱（GC-MS）测定地表水环境中常见VOCs的方法优化研究

[目的]对地表水环境中常见VOCs测定方法进行优化。[方法]采用吹扫捕集一气相色谱质谱（Gc—MS）法,分析地表水源水中多种挥发性有机物,并对方法条件进行了优化整合,以进一步提升分析效率和方法灵敏度。[结果]通过SIM模式测定25种VOCs,空白加标回收率范围为81．5％～123．0％,精密度（相对标准偏差）范围为0．74％一5．62％,方法检出限为0．03-0．59¨g／L,实际水样加标回收率范围为79．5％～125．0％。[结论]吹扫捕集一气相色谱质谱联用法测定水中VOCs,具有准确、可靠、重复性好等优点,适用于地表水环境中VOCs的测定。     

藜草花叶病毒的研究概述

藜草花叶病毒(Sowbane mosaic virus,So MV)是菠菜上的重要有害生物,可通过种子、花粉、昆虫介体和机械等方式传播。随着我国种子、种苗进口量的进一步扩大和产业化种植,藜草花叶病毒传入我国的风险非常大。本文综述了藜草花叶病毒的生物学特性、分布、寄主范围、为害症状、传播途径、检测技术等,论述了藜草花叶病毒传入我国的潜在危害性和风险。     

中草药制剂对泌乳奶牛机体免疫性能的影响

试验采用大青叶、大黄、党参等19味中草药分别组成内服和子宫灌注制剂治疗奶牛子宫内膜炎，并采集颈静脉血测定淋巴细胞刺激指数（SI）和中性粒细胞吞噬力，以观察中草药制剂对奶牛免疫性能的影响。结果显示，中草药制剂均可提高奶牛免疫力，内服中草药制剂应用效果优于子宫灌注制剂（P〈0．05）；复方制剂优于单味中草药制剂（P〈0．05）；复方中草药内服制剂可使淋巴细胞刺激指数（SI）和中性粒细胞吞噬力分别提高53．76％和4．58％（P〈0．01）；复方子宫灌注制剂可使淋巴细胞刺激指数（SI）和中性粒细胞吞噬力分别提高39．58％和1．98％（P〈0．01）。     

Screening and Analysis of Proteins Interacting with TaPDK from Physiological Male Sterility Induced by CHA in Wheat

To further research the regulatory network of pyruvate dehydrogenase kinase (designated as TaPDK) in physiological male-sterility (PHYMS) of wheat induced by chemical hybridizing agent (CHA) SQ-1, an anther cDNA library was constructed, and the proteins interacting with TaPDK were screened via yeast two-hybrid technique. Subsequently, a few candidate proteins in nucleotide expression levels were detected by real-time quantitative PCR. Yeast-two hybrid screening was performed by mating yeast strain Y2HGold containing BD-TaPDK bait plasmid with yeast strain Y187 including anther cDNA library plasmid. Diploid yeast cells were plated on synthetic dropout nutrient medium (SD/-Ade/-His/-Leu/-Trp) (QDO), and further were incubated on QDO medium containing AbA and X-α-Gal. The interactions between TaPDK and the proteins obtained from positive colonies were further confirmed by co-transformation validation. After plasmids DNA were extracted from blue colonies and sequenced, the sequences results were analyzed by bioinformatic methods. Finally, 24 colonies were obtained, including eight genes, namely non-specific lipid-transfer protein precursor (TanLTP), polyubiquitin (TaPUbi), glyceraldehyde-3-phosphate dehydrogenase, proliferating cell nuclear antigen (TaPCNA), CBS domain containing protein (TaCBS), actin, guanine nucleotide-binding protein beta subunit, chalcone synthase, and three new genes with unknown function. The results of quantitative RT-PCR showed that the expression levels of TanLTP, TaPUbi, and TaPCNA were obviously up-regulated in PHYMS anther, and TaCBS expression was only increased at the tricellular stage in PHYMS anther compared with in fertile lines. Whereas, the expression of TaPDK was obviously down-regulated in PHYMS lines. Collectively, these datas indicated that the majority of candidate proteins might be related to pollen abortion in PHYMS lines, which further suggested that TaPDK plays multiple roles in pollen development, besides participating in regulating pyruvate dehydrogenase complex activity.     

广西农产品物流企业WLC品牌元素的选择研究

针对广西农产品物流企业的特点,从众多影响企业品牌建设与发展的因素中,采用AHP-TOPSIS模型选择确定品牌全生命周期阶段对品牌竞争力影响力度最大的品牌元素,以实现品牌的可持续发展。     

4种不同杀虫剂防治淮山斜纹夜蛾药效试验

通过选用4种不同的杀虫剂防治淮山斜纹夜蛾试验，结果表明：药后10d，10亿PIB／mL银纹夜蛾多角体病毒悬浮剂（奥绿一号）处理的防效达100％，0．15％甲氨阿维·1000万PIB／mL苜银夜核悬浮剂（攻蛾）防效达92．8％，15％杜邦安打悬浮剂防效达91．7％，这3种药剂值得在生产上推广应用。     

金针菇航天种菌丝诱变效应的研究

本实验以2个金针菇航天诱变菌种(航金1号和航金2号)和地面对照菌种(江山白)为材料,进行了拮抗实验、菌丝生长速度实验、抗杂性实验和酯酶同工酶分析实验研究,结果表明,金针菇航天种与对照之间有拮抗现象,但无拮抗线。航天搭载菌株的菌丝生长速率比对照快,其中航金1号生长速度最快。航金1号和航金2号均表现出较强的抗杂性,能抑制并盖过霉菌菌丝生长。通过酯酶同工酶聚丙烯酰胺凝胶电泳分析发现,航金1号和航金2号与对照相比都出现了特异谱带,说明经过航天诱变的菌株发生了一定的突变。     

W/O微乳液体系中SA和MS清除·O_2~-的研究

以水溶液体系作为对照,利用分光光度法研究了W/O型微乳液体系中水杨酸（SA）和水杨酸甲酯（MS）清除超氧阴离子自由基（·O2-）的性能.结果发现,在水浴时间为20min、pH值为8.20和邻苯三酚加入量适宜的情况下,W/O微乳液体系中SA、MS对·O2-的清除率分别可达72.92%、81.47%.微小的pH值变化会引起邻苯三酚自氧化速率的显著变化,电解质溶液对其也有明显的影响,但对SA、MS清除·O2-的能力影响不大.     

大青叶中4（3H）喹唑酮体外抗H1N1型流感病毒的作用研究

目的：探讨大青叶中4（3H）喹唑酮体外抗H1N1型流感病毒的作用。方法：用MTT染色法测定大青叶中4（3H）喹唑酮通过不同加药方式对H1N1型流感病毒的体外抑制作用。结果：大青叶中4（3H）喹唑酮对H1N1型流感病毒具有明显的阻断作用和对细胞内病毒有抑制作用。结论：大青叶中4（3H）喹唑酮具有明显的体外阻止H1N1型流感病毒吸附到靶细胞和抑制H1N1型流感病毒增殖的作用。