Quantitative studies on the relationship between plowing into soil of clubbed roots of preceding crops caused by Plasmodiophora brassicae and disease severity in succeeding crops

The effect of the plowing of clubbed roots of cracifers on the population of Plasmodiophora brassicae in soil was quantitatively studied by measuring the number of resting spores produced in the diseased plants. Though the mean number of resting spores per diseased plant increased with the increase of the disease severity, it remained almost identical for the disease severity classified into category 3 among host species and cultivars tested. Mean number of resting spores per diseased plant ranged from 9.3 to 10.9 (log) regardless of the value of the disease index. When the number of resting spores in soil was calculated based on these data and plant cultivation methods, the values were equivalent to 4.8-6.4 resting spores g^-1soil (log)where clubroot disease occurred severely. The value of the disease index of Chinese cabbage plants grown in the pots where clubbed roots of initially grown plants had been plowed into soil (plowing plot) was higher than that in the pots where no plants had been grown (control plot) and where the clubbed roots of initial plants had been removed (removal plot). Though the number of resting spores of P. brassicae in soil decreased by 14% of the inocoKum concentration immediately after the inoculation, the number of spores after the first cultivation in the removal plot was similar to that in the control plot. On the other hand, the number of resting spores in the plowing plot increased significantly compared with that in the control plot. The plowing of clubbed roots into soil resulted in the increase of the population of P. brassicae and disease severity of clubroot in subsequent cultivation in the field. The results corresponded to the values estimated based on the number of resting spores in soil in relation to each value of the disease index.     

植物根系对土壤侵蚀控制机理的研究

介绍了近年来植物根系对土壤侵蚀控制机理的研究进展。在水文效应和机械效应两个方面,植物根系通过增强土壤的抗冲性、渗透能力、抗剪强度以及根系网的固土功能提高土壤的抗侵蚀能力。     

Effects of inbreeding on nodal root system morphology and architecture of white clover (<Emphasis Type="Italic">Trifolium repens</Emphasis> L.)

Plants of white clover (Trifolium repens L.) cultivar Crau, a self-fertile Crau genotype, and nine generations of inbred progeny were raised in sand culture in a glasshouse experiment. Digital images of the root systems were made and root morphological characteristics were determined on all the plants. Root architectural parameters were measured on the Crau parent and the S₁, S₄, S₆, and S₉ inbred lines. The clover roots became shorter and thicker with inbreeding but the number of root tips per plant was unchanged. Root architecture (branching pattern) was largely unaffected by inbreeding. It is concluded that inbreeding white clover will lead to shorter, thicker roots, and reduced nutrient uptake efficiency compared with the parent clover. The degree to which these deleterious traits are overcome during the development of F₁ hybrids needs to be determined.     

基于EIT的土壤中油菜根茎检测

主要应用EIT技术检测土壤中油菜的根茎。搭建16电极数据采集系统,采用相邻法激励测量模式获得成像数据并应用基于MatLab的MNR算法进行图像重建。结果表明:本实验方法能实现油菜根茎的无损检测,可以检测出油菜根茎的位置和大小信息;对干枯的油菜根茎检测效果比新鲜根茎差,而对于腐烂的根茎,不能检测出来。据此,可以对土壤中油菜根茎进行可视化观察,了解其根系生长情况。     

沙棘微繁育苗产业化试验研究——水培沙棘茎段插穗不定根发生的机理

本文用水培方法研究了沙棘短茎段插穗生根的机理。结果表明,在用外源激素处理、控温控湿条件下,沙棘可以用极短的茎段(1～3芽段)插穗产生不定根,进而形成新的植物。沙棘插穗不定根形成有两种方式:一是茎段切面先产生愈伤组织,然后再分化为不定根;二是直接由茎皮层内分生组织产生不定根;且不定根产生具"茎多部位生根效应"。沙棘插穗不定根形成属于"温度敏感型",最适温度为16℃～26℃。外源激素对沙棘短茎段插穗生根表现出良好的诱导效应,而且对插穗苗的生长发育一定的影响,ABT优于GGR;"壮根素"效果优于生根粉,不仅促进发根,而且促进成熟不定根的早期结瘤。     

烤烟幼苗根系在低温胁迫后的形态及生理生态响应

根系作为重要的吸收器官和代谢器官,其生长状况不仅直接控制着植物吸收水分和养分,而且制约着植株地上部分生长的好坏。温度是影响烤烟幼苗根系发育的重要因素之一。南方多数烟区育苗阶段早春低温严重,影响着健壮烟苗的培育。为此,选用云烟87(YY87)、Msk326(K326)和云烟85(YY85)等3个品种烤烟幼苗,在5～7℃条件下分别处理2,4及6d,研究烤烟幼苗根系在低温胁迫后的形态及生理生态响应。结果表明:YY87低温胁迫处理不同时间后,SOD,POD,CAT酶活性均有不同程度上升;K326在处理2 d及4 d后,3种酶活力均较对照明显下降;而YY85的SOD酶活性上升,CAT和POD活性则表现下降。就根系活力而言,YY87在不同低温处理时间均表现为上升;而K326及YY85则表现为短时间(2d)低温胁迫根系活力受到抑制,随着处理时间延长,其根系活力又逐渐增强。低温处理2d后,YY87根平均直径、根表面积和根体积显著下降,但随着胁迫时间延长则恢复正常;K326则在低温处理2d后根直径、根长、根表面积和根体积均达到最大,其后逐渐降低;YY85则表现根表面积和体积均有不同程度下降。由此表明,不同品种幼苗根系受到低温胁迫后其根系形态及生理生态指标存在着不同的响应机制。     

Deficit irrigation based on drought tolerance and root signalling in potatoes and tomatoes

Agriculture is a big consumer of fresh water in competition with other sectors of the society. Within the EU-project SAFIR new water-saving irrigation strategies were developed based on pot, semi-field and field experiments with potatoes (Solanum tuberosum L.), fresh tomatoes (Lycopersicon esculentum Mill.) and processing tomatoes as model plants. From the pot and semi-field experiments an ABA production model was developed for potatoes to optimize the ABA signalling; this was obtained by modelling the optimal level of soil drying for ABA production before re-irrigation in a crop growth model. The field irrigation guidelines were developed under temperate (Denmark), Mediterranean (Greece, Italy) and continental (Serbia, China) climatic conditions during summer. The field investigations on processing tomatoes were undertaken only in the Po valley (North Italy) on fine, textured soil. The investigations from several studies showed that gradual soil drying imposed by deficit irrigation (DI) or partial root zone drying irrigation (PRD) induced hydraulic and chemical signals from the root system resulting in partial stomatal closure, an increase in photosynthetic water use efficiency, and a slight reduction in top vegetative growth. Further PRD increased N-mineralization significantly beyond that from DI, causing a stay-green effect late in the growing season. In field potato and tomato experiments the water-saving irrigation strategies DI and PRD were able to save about 20-30% of the water used in fully irrigated plants. PRD increased marketable yield in potatoes significantly by 15% due to improved tuber size distribution. PRD increased antioxidant content significantly by approximately 10% in both potatoes and fresh tomatoes. Under a high temperature regime, full irrigation (FI) should be undertaken, as was clear from field observations in tomatoes. For tomatoes full irrigation should be undertaken for cooling effects when the night/day average temperature >26.5 °C or when air temperature >40 °C to avoid flower-dropping. The temperature threshold for potatoes is not clear. From three-year field drip irrigation experiments we found that under the establishment phase, both potatoes and tomatoes should be fully irrigated; however, during the later phases deficit irrigation might be applied as outlined below without causing significant yield reduction:

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Potatoes

°

After the end of tuber initiation, DI or PRD is applied at 70% of FI. During the last 14 days of the growth period, DI or PRD is applied at 50% of FI.

•

Fresh tomatoes

°

From the moment the 1st truce is developed, DI is applied at 85-80% of FI for two weeks. In the middle period, DI or PRD is applied at 70% of FI. During the last 14 days of the growth period, DI or PRD is applied at 50% of FI.

•

Processing tomatoes

°

From transplanting to fruit setting at 4th-5th cluster, the PRD and DI threshold for re-irrigation is when the plant-available soil water content (ASWC) equals 0.7 (soil water potential, Ψ_soil = −90 kPa). During the late fruit development/ripening stage, 10% of red fruits, the threshold for re-irrigation for DI is when ASWC = 0.5 (Ψ_soil = −185 kPa) and for PRD when ASWC (dry side) = 0.4 (Ψ_{soil, dry side} = −270 kPa).

The findings during the SAFIR project might be used as a framework for implementing water-saving deficit irrigation under different local soil and climatic conditions.     

A new method for evaluating symplastic cadmium absorption in the roots of Solanum melongena using enriched isotopes 113Cd and 114Cd

Radioisotope techniques are well known as methods for evaluating symplastic ion absorption in roots. In the present study, a new method for evaluating symplastic cadmium (Cd) absorption in plant roots was developed using the enriched isotopes ¹¹³Cd and ¹¹⁴Cd. Seedlings of Solanum melongena were exposed to an enriched isotope solution of ¹¹³Cd at 25°C for 30 min. The roots were excised from each seedling and were then immersed in a cold buffer solution without Cd at 2°C for 120 min to suppress the metabolic activity of the roots. Finally, the roots were treated with a cold buffer solution containing enriched stable isotope ¹¹⁴Cd at 2°C for 120 min, whereby the apoplastically bound ¹¹³Cd was desorbed. We tested the validity of our method for evaluating symplastic Cd in roots compared with the conventional method based on differences in the amount of Cd absorbed at 2°C and 25°C using unlabeled Cd. There was no difference in the symplastic Cd content of the roots between the two methods. These results indicate that it is possible to evaluate the symplastic Cd content in roots using the enriched isotopes ¹¹³Cd and ¹¹⁴Cd.     

新疆野生枸杞愈伤组织分化及植株再生

以新疆枸杞的叶片、茎尖基部为外植体,分别诱导愈伤组织、继代培养和诱导分化,结果表明:①不同外植体愈伤分化率有明显差异:叶片愈伤组织分化率最高为100%,茎尖基部愈伤组织分化率最高为80%。每块愈伤的芽分化数最多可达15～20个。②激素组合影响愈伤分化。诱导分化最适培养基为6 BA2.0mg·L-1 IAA0.5mg·L-1,KT0.5mg·L-1 NAA1.0mg·L-1。③将分化出的无根苗转接到MS无激素培养基上,生根率可达到85%以上。因此叶片为诱导愈伤组织分化的最适外植体。     

科尔沁沙地低缓沙丘主要植被根系层土壤水分特性分析

土壤与植物是SPAC系统的两个主要要素，本文通过对科尔沁沙地低缓固定沙丘主要植被榆树、小叶锦鸡儿根系发育区域内的土壤水分的取样调查，分析了植被周边土壤水分与植物地上生长形态以及地下根系分布间的关系，并分析了沙地土壤水分的空间分布格局及其主要影响因素。