供氮方式对冬马铃薯氮肥利用效率及氮素去向的影响

以马铃薯费乌瑞它为试材,采用田间微区~(15)N示踪技术,研究施N量160kg·hm~(-2)全部基施(T1)、55%基施+45%在齐苗期追施(T2)、55%基施+30%在齐苗期追施+15%在现蕾期追施(T3)3种方式,对冬马铃薯氮肥利用效率及去向的影响。结果表明:马铃薯吸收的N约46%~52%来源于当季施用的氮肥,48%~54%来自土壤和种薯;肥料N利用率为35.16%~39.99%,残留率为47.71%~51.78%,损失率为8.23%~15.50%。3种施氮方式下,肥料N主要残留在0~15cm土层。随施氮时间后移,肥料N残留在0~15cm土层呈上升趋势,在15~45cm土层呈下降趋势。施氮方式对马铃薯干物质积累总量和块茎干物质积累量影响不明显,但T3肥料N利用率、肥料N残留率明显大于T1、T2。因此,综合经济效益和环境效益,T3施氮方式的效果较为理想。本研究为马铃薯氮素养分的有效管理提供了指导依据。     

Herbicide options for effective weed management in dry direct-seeded rice under scented rice-wheat rotation of western Indo-Gangetic Plains

Farmers' participatory field trials were conducted at Madhuban, and Taraori, the two participatory experimental sites/locations of the Cereal Systems Initiative for South Asia (CSISA), a collaborative project of IRRI and CIMMYT in Karnal district of Haryana, India, during Kharif (wet season) 2010 and 2011. This research aimed to evaluate preemergence (PRE) and postemergence (POST) herbicides for providing feasible and economically viable weed management options to farmers for predominant scented rice varieties. Treatments with pendimethalin PRE fb bispyribac-sodium + azimsulfuron POST had lower weed biomass at 45 days after sowing (DAS). At Madhuban, highest grain yield of scented basmati rice (3.43 t ha⁻¹) was recorded with the sequential application of pendimethalin PRE fb bispyribac-sodium + azimsulfuron POST. However, at Taraori, yields were similar with pendimethalin or oxadiargyl PRE fb bispyribac-sodium and/or azimsulfuron POST. Applying oxadiargyl by mixing with sand onto flooded field was less effective than spray applications in non-flooded field. The benefit-cost ratio of rice crop was higher with herbicide treatments at both sites as compared with the non-treated weed-free check except single PRE and POST applications and sequential application of oxadiargyl PRE fb oxadiargyl PRE. In a separate experiment conducted at Nagla and Taraori sites, scented rice cultivars' ('CSR 30′ and 'Pusa 1121′) tolerance to three rates of azimsulfuron (15, 25, and 35 g ai ha⁻¹) was evaluated over two years (2010 and 2011). CSR 30 (superfine, scented) was more sensitive to higher rates (35 g ai ha⁻¹) of azimsulfuron as compared to Pusa 1121 (fine, scented). Crop injuries were 8 and 28% in case of CSR 30; 5 and 15% in Pusa 1121 when applied with azimsulfuron 25 and 35 g ai ha⁻¹, respectively. Azimsulfuron applied at 35 g ai ha⁻¹ reduced yield in both cultivars but in CSR 30 yield reduction was twofold (11.5%) as that of Pusa 1121 (5.2%).     

不同激素对金花茶圈枝繁殖生根的影响

分析NAA、IBA及2种激素等体积混合溶液对金花茶圈枝繁殖生根成活率的影响。结果表明:采用萘乙酸和吲哚丁酸的等体积混合激素溶液1 000~1 500 mg/L能明显提高金花茶圈枝繁殖苗的生根率,达到95%以上。该技术简化了操作程序,可节省人力,在基质方面也节约了生产成本,可直接应用于金花茶苗木大规模生产。     

井渠灌区地下地表水耦合模拟及用水配置研究

针对宁夏惠农区渠井用水比例失调,导致地下水越过适宜埋深造成次生盐渍化的问题。选择惠农区李岗村为试验区,在概化试验区水文地质条件,建立了水文地质概念模型,采用双重节点方式对地表水地下水模型进行耦合,在每个时间段对两者进行联立同时求解,通过HydroGeoSphere进行模型的计算求解,率定和验证模型中的水文地质参数。并通过建立的模拟模型,适时调整用水比例,得到在生态安全埋深下,渠井用水最优比例。     

Comparison of dietary essential amino acid requirements determined from group‐housed versus individually‐housed juvenile bluegill,Lepomis macrochirus

Two 60‐day experiments were conducted sequentially to determine (i) lysine requirement of juvenile bluegill, Lepomis macrochirus based on the dose–response method, (ii) requirements for other essential amino acids (EAAs) using whole‐body amino acid profile and (iii) whether differences in growth rates of group‐housed versus individually‐housed bluegills lead to different lysine requirement levels because of the presence and absence, respectively, of social hierarchies. Seven, semi‐purified, experimental diets (isonitrogenous, isocaloric) were prepared to contain graded levels of digestible lysine (10–31 g kg⁻¹). Experiment‐1 involved group‐housed bluegills (approximately 27 g, n = 10 fish/chamber, 4 chambers/diet) whereas experiment‐2 involved individually‐housed bluegills (approximately 30 g, n = 1 fish/chamber, 14 chambers/diet). Fish were fed twice daily to apparent satiation. Bluegill growth responses in both experiments generally improved (P < 0.05, anova ) with increasing dietary lysine levels from 10 to 16 g kg⁻¹, and then levelled off with further increase in lysine level (P > 0.05). Optimal dietary lysine level (digestible basis) was estimated to be 15 g kg⁻¹ based on broken‐line regression analyses of relative growth rate and feed conversion ratio with no differences being observed between the two rearing methods. Determined dietary requirement levels for other EAAs ranged from 2.4 g kg⁻¹ (tryptophan) to 15.3 g kg⁻¹ (leucine).     

Seismic behavior of reinforced-concrete and steel reinforced concrete ultra short columns

进行了4个钢筋混凝土(RC)超短柱、4个型钢混凝土(SRC)超短柱和6个RC分体柱的滞回性能试验研究.8个超短柱的剪跨比为1;6个分体柱的剪跨比为2.4.试验结果表明,RC超短柱的变形能力不满足现行抗震规范要求.不设剪力连接件的SRC超短柱的破坏模式为黏结破坏,与RC超短柱相比,其抗剪承载力和变形能力并无明显提高;设置剪力连接件的SRC超短柱的破坏模式为斜压破坏,其抗剪承载力和变形能力显著提高,但构件的变形能力仍然难以满足现行抗震规范要求.试验结果还表明,在RC超短柱中开缝,形成剪跨比大于2的RC分体柱,可有效提高柱子的延性和变形能力.对于RC分体柱,随轴压比的提高,构件的抗弯承载力提高,但延性和变形能力下降;当设计轴压比大于0.7时,RC分体柱的变形能力不满足现行抗震规范要求.根据试验结果,提出了SRC超短柱的剪力连接件设计方法和RC分体柱的设计建议.     

Single and Interactive Effects of Temperature and Light Quality on Four Canola Cultivars

Combined effects of temperature and light quality on plants have received little attention. We investigated the single and interactive effects of temperature and light quality on growth and physiological characteristics of four canola (Brassica napus) cultivars – Clearfield 46A76 (cv₁), Clearfield 45H72 (cv₂), Roundup Ready 45H24 (cv₃) and Roundup Ready 45H21 (cv₄). Plants were grown under lower (24°/20 °C) and higher (30°/26 °C) temperature regimes at low red/far‐red (R/FR), normal R/FR and high R/FR light ratios in environment‐controlled growth chambers (16 h light/8 h dark). Higher temperature reduced stem height and diameter; leaf number and area; dry matter of all plant parts; and specific leaf weight, but increased leaf area ratio; and chlorophyll (Chl) fluorescence (Y). Low R/FR increased stem height; Y; and ethylene, but decreased stem diameter; F_v/F_m; Chl a; Chl b; and carotenoids. Among cultivars, plants from cv₄ were tallest with thickest stems and greatest dry matter. None of the main factors affected gas exchange. Higher temperature at high R/FR caused cv₃ to be shortest, whereas lower temperature at low R/FR caused cv₄ to be tallest. We conclude that heat and other stress factors will adversely affect sensitive crops, but tolerant genotypes should perform well under future climate.     

An experimental study of charcoal degradation in a boreal forest

Degradation rates of pyrogenic carbon (PyC) under natural environmental conditions are largely unknown. Here we present results from a field experiment monitoring the change in mass, C- and N concentrations of a variety of charcoal types in a Norwegian boreal forest over a period of 20 months. The charcoal types represent different feedstock tree species, production temperature regimes, and placements in the forest, i.e. above ground, in the humus layer or in contact with the mineral subsoil. The types of charcoal had different initial C concentrations mainly depending on their production temperature. Nevertheless, all types of charcoal at all placements in the forest showed an initial drop in their C concentrations, which subsequently rose back to reach near initial values in part of the charcoal types. In part of the charcoal types, N concentrations decreased throughout the experiment, exhibiting considerable variation among feedstock species, production temperature regime, and placements in the forest. C/N ratios rose especially in charcoal made from wood of Scots pine (Pinus sylvestris L.), and charcoal that had been stored in contact with the mineral subsoil showed the most rapid mass gain. Our results confirm the important influence of production temperature and feedstock type on the degradation of charcoal, but they also show that microbial activity and environmental conditions play significant roles in charcoal degradation and thus for the fate of pyrogenic carbon under natural conditions.     

杜洛克与二花脸杂交猪群体SNP偏分离分析

偏分离（TRD）是生物中普遍存在的现象,本研究拟在高密度基因芯片判断基因型的大规模群体中挖掘猪基因组标记位点的偏分离位点并探究其潜在的遗传机制。本研究用60K Illumina Porcine SNP芯片对1 020头F2资源家系（杜洛克与二花脸杂交F0-F2群体）进行基因型分型,用偏分离分析软件TRDscan （BF>100）和TDT （P<0.01）方法在单个位点上检测偏分离信号,并对二者共有的显著信号位点附近100 kb窗口的基因进行功能分析。此外,本研究还利用单倍型分型的软件包PHASEBOOK采用多位点连锁分析的策略分析了父源和母源配子中的偏分离区域,并在该区域内搜寻潜在的QTLs。结果表明：1）在单位点的偏分离分析中共鉴定到44个显著的偏分离位点,筛选到23个相关基因;对父本和母本特异性偏分离效应进行分析时,分别鉴定到27和35个显著偏分离位点,其中,分别有11和25个位点的100 kb附近有已注释的功能基因。2）基于单倍型多位点连锁的偏分离结果显示,父本显著偏分离位点位于5和13号染色体上,在该偏分离区域内搜寻功能相关的QTL,共搜寻到3个与繁殖性状（木乃伊数、产活仔数、黄体数）有关的QTLs;分析母本偏分离位点时,在4、6和12号染色体上定位到显著偏分离区域,结合已知的猪QTL数据库,共找到了5个与繁殖性状相关的QTLs。本研究利用大规模猪家系数据系统地鉴别了猪基因组的偏分离位点,为解析猪中的偏分离现象和进一步探究其生物学机制提供了一定的参考作用。     

New technique of sex preselection for increasing female ratio in boar sperm model

In this study, we tried to optimize the porcine semen extender conditions to maximize the differences between live X chromosome-bearing (X) spermatozoa and to Y chromosome-bearing (Y) spermatozoa without a decline in the fertility rate at different pH conditions during storage. We observed the viability of X and Y boar spermatozoa in acidic (pH 6.2), original (pH 7.2), and alkaline condition (pH 8.2) for 5 days to investigate the effect of storage conditions on the X to Y spermatozoa ratio. The functional parameters of spermatozoa were also examined to evaluate sperm quality. Sperm motility was preserved at pH 7.2 and pH 6.2 for 3 days, while sperm motility at pH 8.2 decreased significantly after 2 days. Non-capacitated spermatozoa increased while capacitated spermatozoa decreased during storage. Sperm viability decreased significantly duration-dependent under all pH conditions, but there was no significant difference during storage at pH 6.2 and 7.2. The X: Y ratio of live spermatozoa in acidic condition was maximized (1.2:1) without affecting the sperm function and fertility-related protein expression after 2 days compared to original conditions. Moreover, insemination of sows using acidic extender increased the number of female pups on days 1 and 2 of preservation. These results indicate that the production of female offspring may increase when acidic BTS is used for 2 days without affecting the success rate of AI. Above all, this method is simple and economical compared to other methods.