钻石海棠茎段的组织培养技术研究

为了建立钻石海棠的组织培养快繁体系,以钻石海棠单芽茎段为外植体,以MS为基础培养基进行组织培养研究,结果表明:5月份为单芽茎段外植体获取的最佳时间,此时污染率和死亡率都低,分别为15.3%和9.4%;最佳丛生芽诱导培养基为MS+6-BA 1.0mg/L+NAA 0.1mg/L,诱导率为60.7%;最佳增殖培养基为MS+6-BA 2.0mg/L+NAA 0.2mg/L,暗培养2周,增殖系数为4.65;生根培养基以1/2MS+IBA0.2mg/L+NAA 0.2mg/L较好,平均根长为6.3cm,生根率为97.2%。     

Vegetative propagation of twelve fodder tree species indigenous to the Sahel,West Africa

In the Sahel region, many woody species are used as fodder because of their high nitrogen content, especially during the dry season, in contrast to grasses and crop residues. Unfortunately, this resource is being threatened by regular pruning, increasing livestock browsing and impeding their natural regeneration. Therefore, there is a need to find appropriate regeneration options to sustain fodder production. Thus, a series of vegetative propagation experiments were conducted to identify the most appropriate multiplication methods of some of the species used as fodder. This series included testing the effects of naphthalene acetic acid (NAA) and indole butyric acid (IBA) on rooting ability of stem cuttings of 12 species: Afzelia africana, Balanites aegyptiaca, Bauhinia rufescens, Commiphora africana, Faidherbia albida, Ficus gnaphalocarpa, Guiera senegalensis, Kigelia africana, Pterocarpus erinaceus, Pterocarpus lucens, Pterocarpus santalinoides and Terminalia avicennioides. The series also evaluated the impacts of the size (C. africana) and the age (P. erinaceus) of the cuttings on their rooting success. Pterocarpus santalinoides was revealed to be an easy-to-root species, showing a high percentage of rooted cuttings (up to 88±3%), followed by P. erinaceus, P. lucens and B. aegyptiaca (up to 37±4%, 31±5% and 26±6%, respectively), in contrast to A. africana, cuttings of which did not root at all. The application of NAA and IBA enhanced the number of roots and secondary roots per rooted cutting. Large-diameter cuttings (15–20?mm) of deeply planted C. africana gave the highest percentage of rooted cuttings (78.3±4.4%). Tree pruning improved the rooting capacity of cuttings collected from P. erinaceus with one-month resprouts giving the highest percentage of rooted cuttings (50.0±6.7%). These results indicate that P. santalinoides, C. africana, P. erinaceus, P. lucens and B. aegyptiaca are amenable to vegetative propagation as an alternative to sexual propagation.     

浅谈工厂企业绿地景观设计——以珠海科斯特电源有限公司为例

城市化的过程离不开工业经济的发展,在新兴的城市以及在城市新兴的工业园区内,工业用地都占有极大的比重。因此,工厂企业的绿地景观与公共空间、商业空间以及其它城市空间的绿地景观一样是城市绿地景观的重要组成部分,必须引起我们的重视。作者从绿地景观设计的角度出发,结合珠海科斯特电源有限公司绿地景观设计的实例,探讨了工厂企业在绿地景观设计方面的若干问题。     

冬枣树皮疱斑病防治药剂筛选试验

冬枣树皮疱斑病Phoma persicae是近年在冬枣上新发生的一种重要病害,2009～2011年对其开展了室内抑菌测定和田间防效试验。结果表明,室内抑菌效果较好的药剂为苯醚甲环唑、丙环唑、氟硅唑、苯醚甲环唑.丙环唑、恶唑菌酮.氟硅唑,抑菌率分别为98.7%、96.2%、94.9%、93.6%、92.3%;田间防治效果较好的药剂有苯醚甲环唑、嘧菌酯、氟硅唑、中生菌素,防治效果分别为90.2%、87.1%、80.4%、69.1%,可以在防治冬枣树皮疱斑病生产中推广使用。     

湘林黑杨新无性系不同部位水培生根性状研究

对湘林-90等6个杨树新无性系及中汉-17、I-69杨1年生苗,自下而上按4部位分别裁截插穗,进行水培生根试验,探索杨树水培后皮层凸起、先期不定根数量、凸起成根率及根长在无性系、区分段间的分布规律及相关性。     

三花冬青嫩枝扦插繁殖技术

运用单因素试验研究植物生长调节剂、扦插基质和带踵扦插等因素对三花冬青扦插生根的影响,结果表明3个因素对三花冬青扦插生根影响差异显著,采用当年生半木质化插条,黄心土为基质,IAA 250 mg·L-1或IBA 500 mg·L-1蘸根处理15 s,带踵扦插等技术措施,可以显著提高三花冬青嫩枝扦插的生根率,在生产中具有较高的应用价值。     

丹东桧柏半木质化嫩枝扦插技术研究

为探讨丹东桧柏半木质化嫩枝扦插技术,进行了不同部位的插穗、不同生根剂及其不同浓度、不同扦插基质、不同扦插时期的扦插生根试验,结果表明:采条部位上、中、下均可,生根剂以IAA最适合,浓度以100mg·L-1最佳,基质以沙土混合土和炉灰为宜,扦插时期以3月15日为好。100mg·L-1是IAA的最佳浓度,其生根率为97.5%,效果明显。     

油茶扦插生根过程中的营养物质含量变化研究

为了解油茶(Camellia oleifera)扦插生根过程中营养物质含量的动态变化及其与插穗生根的关系,以清水为对照,用IBA、ABT6、NAA生根粉处理插穗,并对油茶嫩枝扦插生根过程中部分营养指标的含量进行了测定.结果表明:外源生长激素提高了插穗生根率,改善了生根性状,其中以IBA处理为最优;处理组与对照插穗的可溶性总糖质量分数、淀粉质量分数随时间变化趋势一致,均呈"下降—升高—下降"趋势,处理组出现的峰值均比对照早且高;总氮质量比变化呈"升高—下降—升高"趋势,处理组全氮含量在根的表达期均低于对照,但生根率均高于对照; C/N值呈现"下降—升高—下降"变化趋势,处理组C/N值变化曲线的峰值显著高于对照,C/N值越高的处理,其生根率越高.     

黄杞容器扦插繁殖技术试验研究

通过正交试验设计L9（34）深入研究了使用不同种类生根促进剂（H ）来处理母株不同采插穗部位的插穗（F ）和插穗不同基部下切口型（E ）对黄杞扦插生根率、生根数及生根长的影响差异。试验结果表明：采用生根促进剂ABT －1＃（H1）＋母株下部采的插穗（F3）＋基部下切口型为双削面（E2）,即 H1 F3 E2组合试验效果最好,其平均生根率高达86．8％、平均生根数8．8条、平均生根长达18．6cm。     

杉木组培无根苗瓶外生根试验

运用正交试验设计探讨了基质、愈伤组织和ABT对杉木组培苗生根的影响。结果表明:基质、愈伤组织和ABT对杉木组培苗的生根率产生显著影响,最佳组合为:基质用红心土∶河砂(3∶1)+愈伤组织部分切除+ABT1#300 mg.L-1。