高等教育地位的提升与高校教师的危机感

以“科教兴国”战略的实施为标志，中国的高等教育已从社会的边缘走向社会的中心。社会对高等教育的旺盛需求，将会持续相当长的时间。随着高等教育的地位的提升，高校教师群体的不同层次均产生了明显的危机感，这种危机感的产生，是高等教育深化改革的必然。     

One-step RT-PCR for the Detection of Infectious Bursal Disease Virus in Clinical Samples

A single-tube, non-interrupted, one-step RT-PCR has been standardized to amplify the hypervariable region of the VP2 gene sequence of infectious bursal disease virus (IBDV). The technique standardized on purified viral RNA was successfully applied to the detection of the virus directly in clinical samples. The amplified products were confirmed to be IBDV specific by their size in ethidium bromide-stained agarose gel, nested PCR and restriction enzyme digestion. Digestion of the amplicons with StyI restriction enzyme also differentiated classical virus from six very virulent field isolates. The sensitivity of the one-step RT-PCR was found to be 0.2 pg of viral RNA.     

A Universal `One-tube' RT-PCR Protocol for Amplifying Isolates of Bovine Viral Diarrhoea Virus

The increase in the knowledge of the genetic variability of BVDV and the identification of some of the genetic determinants of its pathogenicity require robust and practical tools for rapid molecular characterization of the various genotypes of this virus. This study was undertaken to develop a standard protocol for RT-PCR that allows the amplification of various parts of the genome of BVDV without the need for optimizing each individual reaction. The reaction set-up is very flexible because it consists of two pre-mixes. These are a master mix, with all the required reagents except the desired primers, which are the components of the second pre-mix and are therefore easily interchangeable between the different reactions. After adding any primer-containing pre-mix to the fixed master mix, a non-interrupted cycling protocol led to the generation of amplicons of up to 4 kbp in size in amounts sufficient for subsequent sequencing reactions. The method was applied to five different regions of the BVDV genome: (i) the well-known 5-UTR to differentiate genotypes I and II; (ii) the entire E2 gene, or an approximately 550 bp region within the E2 gene, in order to find the molecular equivalent of antigenic varieties; (iii) the entire structural protein coding region covering the Npro, capsid, E ^RNS, E1 and E2 genes; (iv) a 2.1 kbp region embracing the NS2/3 junction which is known to be cleaved in cytopathic biotypes of BVDV; and (v) the region covering the entire NS4B and NS5A/B genes. All six RT-PCRs were successfully applied using (i) primers with lengths of between 20 and 52 nucleotides, (ii) an aliquot of RNA extracted from either 10⁶ infected bovine embryonal lung cells or the same number of leukocytes from viraemic cattle, and (iii) all the genotype I and II strains of BVDV tested. The technique described was used to generate various Sindbis virus/BVDV recombinants. The correct processing of the amplicon-derived E2 glycoprotein of BVDV strain PT810 was demonstrated by its reaction with a monoclonal antibody in an immunofluorescence assay. Given the variety of RT-PCRs tested, we conclude that this universal protocol may be useful with other RNA viruses.     

仲丁威三种测定方法的比较

本文采用反相高效液相色谱法和改进的气相色谱法对仲丁威含量进行了测定,并与ＣＩＰＡＣ采用的正相高效液相色谱法测定了进行了比较。结果表明,反相液相色谱法与正相液相色谱法等效,气相色谱法经改进后降低了仲丁威的热分解,三种方法对仲丁威原药及乳油含量的测定结果的相对误差在允许的范围之内。     

推进碳汇林业发展的几点思考

本文结合吉林省实际,重点阐述了碳汇林业发展的必要性和重要性,指出了科技、人才对碳汇林业发展的支撑作用,强调了科技研发环节前移和发挥学术组织作用的战略选择。     

儒家"重农"思想研究——以《齐民要术》为例

儒家文化在传统文化之中居于主流地位,其社会历史观具有明显的"重农"特征.儒家社会历史观中的"重农"思想对<齐民要术>农学思想产生了极其重要的影响,并被<齐民要术>具体化为"食为政首"思想、"教民致富"思想以及"家理移官"思想三个方面,<齐民要术>的农学思想也因而具有鲜明的儒学化特征.     

辐射事故对公众心理影响及消除方法

此文章系为弄清辐射事故对公众心理的影响，充分研究怎样消除辐射事故信息带来的负面影响，使核事业稳步进展并得到公众支持而完成的。我们已经确定，公众甚至事故发生后的救援人员，往往都对“放射性泄漏”普遍存在着恐惧心理，这种负面影响若不采取相应措施予以减轻乃至消除，将极大的限制核事业的发展。经研究判断，辐射事故会对不同人群带来多种影响。针对此影响的成因与解决方案，本文给出了一些初步的研究结果。     

革命的出场与反思的缺席——论解放区文学的示众描写

作为民众翻身的一个重要环节,解放区文学对示众的描写一改鲁迅等人的批判色调,充满肯定意味。这种新型示众不仅颠覆了传统的政治权力结构,使民众取代地主成为社会的主人;而且解构了传统的社会关系结构,使阶级意识取代传统人情成为社会人际关系构架的基础。然而,由于作家们反思意识的缺席,过于强调阶级怨恨与阶级暴力的革命性与合理性,忽视了程序正义的重要性,使得这种示众描写潜含着人性与社会危机。     

一种逆M序列的动态显示方法

根据逆M序列产生原理，实现了计算机系统辨识仿真时逆M序列的产生和动态显示,并通过实例证明了程序的正确性和实用性.