Evaluation of emmer wheat and other Triticeae for resistance to Russian wheat aphid

Summary During the winters of 1990/91 and 1991/92, 181 accessions of Triticum dicoccon Schrank from the CIMMYT gene-bank were screened in the field for resistance to Russian wheat aphid, Diuraphis noxia (Kurdjumov). Accessions were sown in hill plots of 10 seeds and artificially infested with D. noxia at the two-leaf growth stage. Hills were visually assessed for damage at tillering, booting and heading. Entries differed significantly in their reaction to D. noxia, and severity of symptoms increased with time. Twenty four of the entries were highly resistant to the aphid. In winter 1991/92, 807 accessions of wild and cultivated wheats (26 species) and synthetic hexaploids were screened similarly for resistance to D. noxia. A large number of A-genome species were resistant, while few D-genome species were identified as resistant. These newly discovered sources of resistance can be used to expand the genetic base of resistance to D. noxia in both bread (T. aestivum L.) and durum wheats (T. turgidum L. convar. durum (Desf.) Mackey).     

膜片钳技术及其应用

膜片钳技术是在电压钳基础上发展的，将尖端为1μm的玻璃微电极吸附到细胞表面，使微电极与细胞膜形成高阻封接，从而可记录到膜上pA级的离子通道电流，目前已发展了多种记录模式，对细胞进行电压和电流钳制，以观察各种离子通道及其调控。此外膜片钳技术与胞内纤维荧光测钙技术、膜电容监测技术、碳纤维电极局部电化学微量检测技术、逆转录多聚酶链式反应技术（PCR）结合，在药理学、病理学、神经科学、脑科学、细胞生物学和分子生物学等生物科学方面，得到越来越广泛的应用。     

谷氨酰胺的抗疲劳生化机制研究

用两批各 48只小鼠分 4组 (n =12 )每天分别灌喂生理盐水和 3 4mmol·kg-1的谷氨酰胺、谷氨酸和天冬酰胺 ,连续 10d。结果显示 ,三者都可提高小鼠血清、肝和骨骼肌中的肌酸磷酸激酶活性 ,降低乳酸脱氢酶活性 ,并显著增加肝、骨骼肌糖原积累。其中有相同碳架结构的谷氨酰胺、谷氨酸能极显著提高小鼠的游泳耐力 ,但与谷氨酰胺有相等酰胺氮的天冬酰胺作用不强。结果提示 ,谷氨酰胺提高机体抗疲劳作用与其碳架结构有关     

淮北平原砂姜黑土玉米产量与土壤性质的区域分析

以淮北平原作为调查区域,对砂姜黑土区48个样点的玉米产量及0～20 cm耕层土壤性质进行了分析.结果表明:在涡阳县东、西部,蒙城县南部和怀远县北部,玉米产量多在8.7 t/hm2以上,土壤容重较小;而在蒙城县北部,玉米产量多在7t/hm2以下,土壤容重最大,平均达到1.6 g/cm3以上.涡阳县土壤有机碳含量最高,平均...     

Evaluation of pedotransfer and measurement approaches to avoid soil compaction

On-farm approaches are needed to help farmers avoid soil compaction. It is the purpose of this paper to document the experience of using the Horn and Fleige [Horn, R., Fleige, H., 2003. A method for assessing the impact of load on mechanical stability and on physical properties of soils. Soil Till. Res. 73, 89–99] procedures to develop improved guidance to help farmers avoid compaction in agricultural operations in the Commonwealth of Pennsylvania, USA. A soil characterization database for the Commonwealth of Pennsylvania, USA, was used to provide input to the Horn and Fleige [Horn, R., Fleige, H., 2003. A method for assessing the impact of load on mechanical stability and on physical properties of soils. Soil Till. Res. 73, 89–99] approach to estimate the pre-consolidation stress and the maximum depth of compaction for 29 agricultural soils in Pennsylvania. The Horn and Fleige [Horn, R., Fleige, H., 2003. A method for assessing the impact of load on mechanical stability and on physical properties of soils. Soil Till. Res. 73, 89–99] approach was tentatively validated using previously measured pre-consolidation stress or penetration resistance values measured on five of the 29 soils. The estimated maximum depth of compaction indicated that an 89-kN (10-ton) axle load was excessive in almost all cases for soils at matric potentials of −33 and −6 kPa for both tillage and no-till management. A 53-kN (6-ton) axle load was acceptable for most cases when tillage was planned to a 0.20-m depth, but was excessive in most cases for no-till management at a matric potential of −6 kPa while mostly acceptable for no-till management at a matric potential of −33 kPa. Penetration resistance measurements are recommended to decide when a load is excessive.     

Restoration of woodpasture on former agricultural land: The importance of safe sites and time gaps before grazing for tree seedlings

Woodpastures (open, grazed woodlands with a mosaic of grassland, shrub and tree patches) are of high biological and cultural value and have become a threatened ecosystem in Europe. Spontaneous tree regeneration in the presence of large herbivores, is an essential process for management and restoration of this structurally diverse habitat. We examined the suitability of five vegetation types (grasslands, ruderal vegetations, tall sedges, rush tussocks and bramble thickets), grazed by large herbivores, for tree regeneration. We hypothesized that bramble thickets and tall herb communities operate as safe sites for palatable tree species through the mechanism of associational resistance. We set up a field experiment with tree seedlings in grazed and ungrazed conditions and recorded mortality and growth of seedlings of two palatable tree species (Quercus robur and Fraxinus excelsior) during three growing seasons. In the same experiment, we studied the effect of a two year’s initial time gap before grazing.Bramble thickets were suitable safe sites for survival and growth of seedlings of both species. Tall sedges, soft rush tussocks and ruderal vegetations with unpalatable or spiny species provided temporal protection, allowing seedlings to survive. Tree regeneration in livestock grazed grassland was highly constrained. Rabbits may undo the nursing effects of bramble thickets. The first year’s survival is of major importance for the establishment of trees. Subsequent grazing affects growth rather than survival. A two year’s initial time gap before grazing, had positive effects on survival, but did not enhance outgrowth of unprotected trees.     

Effects of tetracycline on the soil microflora: function,diversity, resistance

Background  Tetracycline is a widely used antibiotic in animal production. Significant amounts of the substance reach the soil via feces, urine and manure application. As tetracycline is a persistent compound with antibacterial activity, its presence in soil may have undesired direct and indirect effects. These have been investigated so far focusing on effects on selected microbial functions. Objectives  The aim of the present study was to obtain comprehensive information on potential effects of tetracycline on the soil microflora under environmentally relevant conditions. The investigations included function and structure of the microbial biocoenosis and the distribution of resistance genes. Methods  Pig manure rich in tetracycline resistance genes was applied to a sandy soil. This soil as well as an unamended soil were additionally treated with several concentrations of tetracycline. The spiked soils were incubated in outdoor lysimeters for several months. Substrate induced respiration, PLFAs, ten selected resistance genes, and the concentrations of tetracycline were determined. Results  The test concentrations, though far exceeding environmental relevance, caused only small effects. An establishment of resistance could not be detected. Applied resistance genes were not detectable at the end of the study even in the presence of added tetracycline. Conclusion  Due to the high sorption capacity of the antibiotic, environmentally relevant concentrations of tetracycline do not seem to cause undesired effects on the soil microflora.     

AM 菌根真菌诱导对提高玉米纹枯病抗性的初步研究

试验研究玉米接种摩西球囊霉后对纹枯病抗性反应的结果表明,接种摩西球囊霉能明显减轻玉米纹枯病的发病率和病情指数,减轻病害。接种摩西球囊霉还能促进玉米营养生长,但立枯丝核菌侵袭会降低菌根的侵染率,表明摩西球囊霉与立枯丝核菌间存在相互作用。     

模拟氮沉降对白羊草地群落特征及其坡面流水动力特性的影响

大气氮沉降的增加可能会通过草地群落进而影响坡面流水动力特性。选取黄土高原典型地带性草本白羊草为研究对象,通过设置不同施氮处理（0,2.5,5,10 g/(m2·a),以N计,下同）模拟氮沉降,采用人工模拟降雨,探究土壤有效氮升高对白羊草群落季节变化及坡面流水动力特征的影响。结果表明,一定程度的施氮可有效增加白羊草盖度,促进藻结皮的生长,但会抑制苔藓结皮的发育。施氮使Darcy-Weisbach阻力系数和曼宁糙率系数分别降低了68.6%～71.5%和44.7%～47.4%;平均流速加快了32.0%～44.0%,径流深减小了25.1%～28.7%,同时,使径流剪切力和水流功率显著增加,过水断面单位能减少。随着季节变化,9月白羊草群落坡面Darcy-Weisbach阻力系数和曼宁糙率系数分别较6-8月显著增加了220.2%～496.9%和79.5%～139.4%,平均流速减缓了23.5%～29.7%,径流深增加了36.4%～66.9%,径流剪切力和水流功率均显著降低,过水断面单位能增加。降雨强度对坡面流特征也存在显著影响。随着降雨强度的增大,坡面流阻力减小,进而导致流速、径流深及水流功率增加。草地具有良好的调控坡面流的作用,然而大气氮沉降的增加可能会加剧草地群落坡面土壤侵蚀。     

上海地区甜菜夜蛾的抗药性及防治

通过浸渍法测定上海地区甜菜夜蛾(Spodoptera exigua)对有机磷、拟除虫菊酯类、昆虫生长调节剂类(IGRs)以及一些新型药剂的抗性,结果表明:甜菜夜蛾对乙酰甲胺磷和氰戊菊酯的抗性分别为2 731.2和619.5倍的超高抗水平。昆虫生长调节剂如抑太保、米满等药剂对甜菜夜蛾抗性表现较好,是防治甜菜夜蛾的理想药剂,但近几年的敏感性有所下降,应注意抗药性抗性的产生和加剧。田间药效与室内毒力测定结果显示趋势一致。除尽、安打、氟铃脲、甲胺基阿维菌素苯甲酸盐和病毒制剂等是目前防治甜菜夜蛾的良好药剂。