一种新型钌联吡啶衍生物的合成

提出了基于离子对作用合成新型四苯硼酸化钌联吡啶电致化学发光试剂的方法,并通过紫外、荧光、核磁等谱学手段对其进行了表征。     

乳酸菌奶粉保护剂的研究

利用保护剂对乳酸菌的保护作用,通过梯度实验和正交试验选出保护剂的最佳添加量和最佳复配组合。使奶粉中的乳酸菌具有较高的活菌存活率。试验表明保护剂最佳复配组合为:20g/L脱脂奶粉,5g/L葡萄糖,5mL/L甘油。从而生产出来的乳酸菌奶粉经冲调后,其活菌率达到86.80%,酸度为102°T。     

对抗生素替代品的研究

本文简要地论述了近些年来抗生素替代品的研究情况,主要包括：益生茵、化学益生素、酸化剂、微生物培养物、鸡卵黄免疫球蛋白和中草药添加剂。     

海水养殖废水中氨氮测定方法的影响因素及改进研究

采用酒石酸钾钠溶液-氢氧化钠溶液作为屏蔽剂排除海水中钙、镁离子对测定的干扰,将纳氏试剂比色法用于对海水养殖废水中氨氮的测定,最佳测定条件为200 g/L的氢氧化钠溶液用量2.5 mL,纳氏试剂1.5 mL,显色时间25 min.该方法对盐度为10～32的海水可以直接测定,氨氮质量浓度在0.01～0.04 mg/L符合比尔定律,检出限为0.01 mg/L,加标回收率在91%～108%.     

An arabinogalactan-protein from whole grain of Avena sativa L. belongs to the wattle-blossom type of arabinogalactan-proteins

An arabinogalactan-protein (AGP) from whole grain of oat (Avena sativa L.) has been isolated for the first time by double precipitation with β-glucosyl Yariv reagent and characterized with regard to its polysaccharide and protein part. The large carbohydrate moiety is rich in galactose (63.0% w/w) and arabinose (32.8% w/w) and free of uronic acids. Linkage analysis of AGP and its products obtained by partial acid hydrolysis as well as enzymatic degradation with specific recombinant enzymes revealed that the carbohydrate moiety consists of a 1,3-Galp backbone and is linked in position 6 to short 1,6-Galp chains, terminating in Araf. In the protein part, high contents of alanine, hydroxyproline and serine could be found which are typical for AGPs. The molecular mass of AGP was determined to be 83 kDa. The carbohydrate moieties, released by alkaline degradation of the protein part, had a size of about 7 kDa. Consequently, the overall structure of the AGP from oat could be assigned to be consistent with the wattle-blossom model of AGPs.     

欧美杨溃疡病防治的初步研究

为探索防治欧美杨溃疡病的方法,采用平板稀释法,从欧美杨溃疡病病株的根际土壤中分离出86株细菌菌株。经平板对峙培养,得到5株对欧美杨溃疡病原菌(Lonsdalea quercina)有拮抗作用的菌株。室内离体枝条试验和田间杨树防治试验结果表明,以菌株P2和P4的防效较好,通过浇根处理,室外防效分别达到51.8%和48.6%。经鉴定菌株P2和P4分别为枯草芽孢杆菌Bacillus subtilis和委内瑞拉链霉菌Streptomyces venezuelae。用中生菌素、农用链霉素、新植霉素、多抗霉素等4种抗生素类药剂和45%代森铵水剂、80%乙蒜素乳油杀菌剂在室内做抑菌试验,中生菌素、新植霉素对病原菌无明显的抑制效果,其他4种有一定的抑制作用。其中农用链霉素100倍稀释液的防效较好,经浇根处理,室外防效达38.2%。试验为欧美杨溃疡病的防治提出了初步的方法。     

显色剂加入方式及结果计算方法对土壤磷酸酶测定结果的影响

为探讨有色显色剂的加液方式及加入量对显色后溶液吸收值的影响、标准曲线的绘制及结果计算方法对土壤磷酸酶测定值的影响,取广东橡胶园0～20 cm土样为材料,以磷酸苯二钠为基质,酶解土壤释放出酚,与显色剂氯代二溴对苯醌亚胺反应,以标准曲线法求出样品测试液的酚浓度,比较以不同加液方式加入不同量的显色剂对溶液吸收值的影响,同时比较用直线或一元二次曲线等两种标准曲线回归方程对样品测试液酚浓度的影响。结果表明：以胶头滴管滴加或以移液枪用多次重复使用过的吸嘴加入显色剂,显色后溶液吸收值变异系数达3.33%～6.44%,而以活塞式定量加液器加入,其变异系数在1.28%以下,且随着加入量增大变异系数降低至0.51%,重现性好;当配制的标准曲线浓度范围较宽且相应吸收值较高(接近0.9),以曲线法求得的标准曲线回归方程决定系数(R2＝0.999 8)大于直线回归的(R2＝0.999 7),且曲线法求出的土壤无基质对照及试剂空白酚浓度比直线法更有实际意义。     

昭通烤烟加酶发酵处理的效果分析

对昭通烤烟加酶发酵处理和对照样品的致香物质、常规化学成分和评吸比较进行分析,分析结果表明,①加酶发酵对烤烟常规化学成分影响不明显。②处理样品的叶绿素和类胡萝卜素降解物明显高于对照。③加酶发酵促进了烟叶残存叶绿素向新植二烯等多种香气物质转化并积累,促进了类胡萝卜素向巨豆三烯酮等多种香气物质转化积累,有利于降低烟叶青杂气,有利于提高烟叶的香气量和香气质。本试验结果还表明,烤烟加酶发酵具有专性转化的工业价值,但仍需进一步的工业验证。     

兔眼蓝莓嫩枝扦插繁殖技术

研究不同基质、不同时期、不同生根剂浓度处理对4个兔眼蓝莓品种嫩枝扦插生根的影响,结果表明：4个品种嫩枝扦插综合生根质量依次为46．43、37．38、20．23、16．55;扦插时间以6月初生根率最高;嫩枝扦插以V红土：V草炭土：V珍珠岩=2：1：1的混合基质生根最好;不同生根剂浓度处理中,质量分数为100×10^-6的ABT1号生根粉有利于灿烂、粉蓝和杰兔插条生根,质量分数为50×10^-6的ABT1号生根粉有利于园蓝生根。     

Development and characterization of stable cell lines constitutively expressing the porcine reproductive and respiratory syndrome virus nucleocapsid protein

Despite global efforts to control porcine reproductive and respiratory syndrome virus (PRRSV) infection, the virus continues to cause economic problems in the swine industry worldwide. In this study, we attempted to generate and characterize a panel of stable BHK cell lines that constitutively express the nucleocapsid (N) protein of type 1 or type 2 PRRSV. The established BHK cell lines were found to react well with N-specific antibodies as well as the hyperimmune serum of pigs raised against each genotype of PRRSV. Taken together, the data implicate a potential usefulness for the newly generated stable cell lines as a diagnostic reagent for PRRSV serology.