球虫感染后兔圆小囊病理组织学变化

为研究圆小囊在家兔球虫感染后的病理变化,试验收集自然感染病例的圆小囊,按HE染色石蜡切片方法制作病理组织切片,观察自然感染球虫后家兔圆小囊的组织病理学变化,并与正常圆小囊组织进行比较。结果表明,球虫感染病例的圆小囊集合淋巴小结生发中心反应活跃,粘膜杯状细胞和固有层散在淋巴细胞明显增多,淋巴小结周边及中心多见吞噬细胞成团聚集,或形成大的多核巨细胞,而集合淋巴小结结构基本完整.该研究结果说明圆小囊的局部免疫机制可能在机体抗球虫免疫中起着重要作用。     

伊普吕肉兔血浆生长激素(GH)水平及其影响因素研究

选用4月龄伊普吕肉兔12只,雌雄各半,分别于白天(13:00)、夜晚(0:00)、饥饿24h、热应激(38℃,15min)状态下经耳缘静脉采血,以放射免疫分析法检测生长激素(GH)水平.试验结果显示,该品种兔血浆GH水平白天((0.80±0.08)μg/L)高于夜晚((0.73±0.05)μg/L);应激((0.93±...     

吡喹酮脂质体对兔肝功能的影响

将吡喹酮脂质体按 4 0mg/kg经耳静脉注入试验兔 ,于给药前及给药后 2 4 ,4 8,72h测定肝功能指标 .所得数据经方差分析表明 ,给药前后GOT ,GPT ,麝香草酚浊度、硫酸锌浊度、黄疸指数、总蛋白、清蛋白含量及清球比例等指标无显著性差异 (p >0 .0 5 ) ,说明吡喹酮脂质体对兔肝功能无明显影响     

肉兔部分血浆蛋白多态性与生产性能关系的研究

以 8个品种 (杂交组合 )的肉兔为试材 ,利用 PAGE对血浆运铁蛋白 (Tf)、铜蓝蛋白 (Cp)、酯酶 -1 (Es-1 )和淀粉酶(Amy) 4个位点的蛋白多态性进行测定 ,并与其生产性能进行比较。结果表明 :Cp、Es-1、Amy 3个位点具有蛋白多态性。蛋白多态性与生产性能有密切的关系 ,Cp的 3种基因型对 1 0 0日龄体重、日增重均呈 AA相似文献   

家兔血清LDH同工酶电泳分析

利用不连续梯度聚丙烯酰胺圆盘凝胶电泳，对８个家兔品种血清的ＬＤＨ同工酶进行电泳分析表明，家兔血清中的５种ＬＤＨ同工酶酶谱的区带分布具有种的特异性；以Ｂ亚基占优势，Ａ，Ｂ亚基的比率可反映家兔的亲缘关系，并据此对家兔品种组合杂种优势进行了估测。     

齐卡生长兔日粮适宜能量,蛋白水平的 研究

试验用齐卡新西兰（Ｎ）和德国合成白兔（Ｚ）断奶兔各１８４和１５２只及两种能量（ＤＥ１２兆焦／公斤，１０兆焦／公斤），蛋白平（１８％，１５％），考察了不同日粮营养水平组合，品系与日粮的互作和能量，蛋白互作对齐卡生长兔的影响。结果表明：齐卡兔不同品系的生长速度不同齐卡新丁日增重极显著高于德国合成白兔（２６．６：２１．３克，Ｐ＜０．０１）。上试初始重对日增重无明显影响。不同粮养水平的日粮，日增重亦高。日     

饲粮中胆碱添加水平对3～5月龄獭兔肝脏脂肪代谢的影响

本试验旨在研究饲粮中胆碱添加水平对3~5月龄獭兔肝脏脂肪代谢的影响。选用200只体重相近的3月龄獭兔,随机分成5组(每组40个重复,每个重复1只),分别饲喂在基础饲粮基础上添加0(对照)、200、400、600和800 mg/kg的50%氯化胆碱(折算为胆碱的添加水平后分别为0、87、174、261和348 mg/kg)的试验饲粮,试验饲粮实测胆碱水平分别为812、902、993、1 086和1 170 mg/kg。预试期7 d,正试期53 d。结果表明:饲粮中胆碱添加水平显著影响肝脏中单不饱和脂肪酸和多不饱和脂肪酸的含量(P0.05)。与对照组相比,饲粮中添加87、174、261和348 mg/kg的胆碱显著增加血清中类胰岛素样生长因子1(IGF-1)和瘦素(LEP)的含量(P0.05),饲粮中添加174和261 mg/kg的胆碱显著增加血清中生长激素(GH)的含量(P0.05),饲粮中添加261和348 mg/kg的胆碱显著增加血清中脂蛋白酯酶(LPL)的活性和极低密度脂蛋白(VLDL)的含量(P0.05),饲粮中添加261 mg/kg的胆碱显著提高肝脏中肉碱棕榈酸转移酶1(CPT1)m RNA的表达水平(P0.05)。综上所述,饲粮中添加261 mg/kg的胆碱(饲粮实测胆碱水平为1 086 mg/kg)改变了獭兔肝脏中脂肪酸组成,促进了肝脏中脂肪酸氧化关键基因CPT1的表达,同时加强了肝脏中甘油三酯(TG)的输出过程。     

Growth performance,carcass quality,biochemical and haematological traits and immune response of growing rabbits as affected by different growth promoters

The aim of the article was to study the effect of different growth promoters on growth performance, carcass quality, biochemical and haematological traits and immune response of growing rabbits. A total number of 105 male growing NZW rabbits during 35–91 days of age were randomly distributed among 7 groups fed the same basal diet and submitted to different dietary treatments: the first group was unsupplemented and used as control; the other groups were supplemented respectively with bee pollen and/or propolis at 200 mg/kg BW of each and inulin and/or mannanoligosaccharides (MOS) at 35 mg/kg BW of each. Body weight gain, daily feed intake, feed conversion ratio (FCR), biochemical and haematological blood parameters, carcass characteristics, histological studies of ileum and spleen and economical efficiency were measured. Bee pollen administered alone or with propolis significantly (p < 0.01) the body weight gain and improved FCR in respect of the control group. Inulin with MOS significantly improved just FCR than the control group. Bee pollen with propolis and MOS supplemented‐groups resulted in significantly higher (7.96 and 8.41% respectively) white blood cells compared to the control group. Plasma total cholesterol was significantly higher for the MOS group in respect of the control, bee pollen, inulin and inulin with MOS supplemented‐groups. Propolis resulted in significantly higher dressed carcass percentage than the control group and higher carcass index than only bee pollen with propolis supplemented‐group. Bee pollen, in particular if in combination with propolis, could be used as a supplement in the growing rabbits during days 35–91 of age with positive effects on growth rate and feed conversion ratio.     

Ultrasound‐assisted periconal ocular blockade in rabbits

ObjectiveThis study aimed to evaluate the benefit and specifically the feasibility of using ultrasound in ophthalmologic periconal block, and the occurrence of complications.Study designProspective experimental study.AnimalsTen healthy New Zealand White rabbits (6–8 months of age), weighing 2.0–3.5 kg.MethodsRabbits were anesthetized by intramuscular injection of acepromazine (1 mg kg⁻¹), ketamine (30 mg kg⁻¹) and xylazine (3 mg kg⁻¹). Ultrasound-assisted periconal block with lidocaine was performed on 18 eyes. Intraocular pressure was measured by applanation tonometry whereas corneal sensitivity was assessed using an esthesiometer, before and after each periconal anesthesia.ResultsIn all 18 eyes, it was possible to adequately visualize the needle shaft within the periconal space, as well as muscular cone, optic nerve and local anesthetic solution spread. Lidocaine 2% without epinephrine (0.79 ± 0.19 mL) was injected into the periconal space. There was no statistical difference between the intraocular pressure (mean ± SD) measured before (10.9 ± 2.9 mmHg) and after (11.9 ± 3.8 mmHg) the periconal anesthesia (p = 0.38). The effectiveness of the ultrasound-assisted technique was shown according to the values for corneal sensitivity, assessed before and after periconal anesthesia (p < 0.0001). Complications were not observed in this study.ConclusionsEye ultrasonography allowed visualization of all anatomic structures necessary to perform a periconal block, as well as the needle insertion and anesthetic spread in real time. Further studies are required to prove the real potential of ultrasound for reducing the incidence of complications associated with ophthalmic blocks, especially when anatomic disorders of the eye could potentially increase the risk.Clinical relevanceUltrasonography is a painless, noninvasive tool that may improve safety of ophthalmic regional blocks, potentially by reducing the prevalence of globe perforation or penetration of the optic nerve associated with the needle-based techniques.     

Effects of propofol on ultrasonic indicators of haemodynamic function in rabbits

OBJECTIVE: To evaluate the cardiovascular effects of intravenous propofol in rabbits. STUDY DESIGN: Randomized, prospective, experimental study. ANIMALS: Thirty-one female New Zealand White rabbits. METHODS: Rabbits were allocated to one of two groups [propofol (P) or conscious (C)]. In C (n = 16) vascular dimensions were measured using ultrasound of the left common carotid artery (ACC) and the abdominal aorta (AA). Group P (n = 15) received propofol 4.0-8.0 mg kg(-1) intravenously (IV). Anaesthesia was maintained with propofol at 1.2-1.3 mg kg(-1) minute(-1). Subsequently, three propofol injections (8 mg kg(-1)) were given. Before and for 10 minutes after each injection the following vascular and haemodynamic variables were recorded (a) at the ACC after the first injection; and (b) at the AA after the second injection: vessel diameter [D, (mm)], peak systolic, minimum diastolic, end-diastolic and average blood flow velocities [psBFV, mdBFV, edBFV, Vave (cm second(-1))], average volumetric flow [VFave (mL s(-1))], resistance index (RI) and pulsatility index (PI) mean arterial pressure (MAP), heart rate (HR), arterial oxygen saturation (SpO(2)) and end-tidal CO(2) (Pe'CO(2)). Echocardiography was performed after the third propofol bolus injection to investigate changes in cardiac parameters [fractional shortening, FS (%)]. RESULTS: Intravenous propofol injections caused a significant decrease in vessel diameter, volumetric flow and edBFV, and significant increases in psBFV, RI and PI. Baseline levels for vessel diameter and psBFV were restored 6-8 minutes after injection. Propofol injection decreased FS significantly by 7 minutes after injection while MAP and HR were significantly reduced for 4 minutes. CONCLUSION AND CLINICAL RELEVANCE: Injections of propofol (8 mg kg(-1)) produced an immediate, transient decrease in vascular diameters, a significant decrease in ventricular performance and an increase in peripheral vascular resistance (ACC and AA). Propofol should probably not be or only carefully used in rabbits with ventricular dysfunction.