Traumatic myocardial laceration as a result of suspected cranial migration of a sewing needle from the stomach of a dog

This report describes a myocardial transdiaphragmatic foreign body as a consequence of a suspected cranial migration of a sewing needle from the stomach of a dog. Surgical removal of myocardial transdiaphragmatic foreign bodies may be associated with significant haemorrhage that requires immediate surgical action, so direct visualisation of the retrieval of a myocardial foreign body is mandatory. A combination of caudal midline sternotomy and cranial coeliotomy approach with diaphragmatic split allowed good visualisation and management of the haemorrhage associated with the foreign body removal in this case.     

高粱(Sorghum vulgare Pers.)营养器官中的硅质细胞与硅质体

本文着重讨论高粱硅质细胞的形态、排列方式和分布数量。幼苗三叶期的幼叶硅质细胞为葫芦形,排列成1—2行。成长植株的中、高位叶硅质细胞成马鞍形,通常与栓质细胞成对,与长细胞相间排列成多种图形。硅质细胞分布数量:低位叶少,中、高位叶增多;叶下表皮多于上表皮;尤以贴叶脉下表皮、主脉下表皮、大平行侧脉下表皮多于其它部     

Chronic Copper Poisoning in Sheep: Structural Changes in Erythrocytes and Organs

Four cases of experimental copper poisoning in sheep were examined. Light microscopical and ultrastructural alterations of erythrocytes were observed a few hours before a significant haemolysis was evident. Heinz body formation in otherwise unchanged red cells was the first morphological alteration observed. The Heinz bodies were predominantly membrane-attached. During the haemolytic crisis severe erythrocytic distortion, structural membrane alteration and Heinz body-containing ghost cells were observed. Erythrophagocytosis was mainly located to the RE cells of the spleen. Intrafollicular necroses were found in all histological sections from the spleen. Pathological changes in liver and kidney were comparable to those of earlier reports, comprising hepatocellular and renal tubular necrosis.     

油棕甾醇合成关键酶基因DWF5的克隆及表达分析

DWARF5（DWF5）基因,编码7-脱氢胆固醇还原酶（7-dehydrocholesterol reductase）,是甾醇合成的关键酶,通过调控甾醇的合成参与植物油体（oil bodies,OBS）的形成。为了探究DWF5基因对油棕果肉中油体发育的影响,以‘热油4号’为材料,提取油棕果肉的RNA,以反转录的cDNA为模板进行基因的全长克隆,获得2个EgDWF5基因并分别命名为EgDWF5-1和EgDWF5-2。运用生物信息学的方法对其理化性质、结构特点和亲缘关系等进行分析预测,利用实时荧光定量PCR对其在油棕的根、茎、叶、花及花后不同时期的果实进行表达水平分析。结果表明：EgDWF5-1和EgDWF5-2基因编码的氨基酸数量分别为434个和374个,相对分子质量为49.71 kDa和42.68 kDa,等电点为8.61和8.69,其蛋白不稳定指数为38.60和38.13,脂肪族指数为98.80和96.68,总平均亲水性为0.347和0.313,均具有疏水性。EgDWF5与水稻和玉米亲缘关系最近,与其他物种关系较远。qRT-PCR分析表明,EgDWF5基因在油棕的各个器官均有表达,其中EgDWF5-1基因在果肉中高表达,显著高于根、叶、花等部位,其表达趋势为花后12~18周升高,至20周时达到最高后表达量逐渐降低,这与油棕果肉中油体的发育趋势基本一致。EgDWF5-2基因在根和茎中高表达而在果肉中表达量低,其果肉中表达趋势为花后12~18周逐渐降低。因此,推测EgDWF5-1参与调控油棕的油体发育,EgDWF5-2未参与该调控而可能在油棕的生长发育中发挥功能。本研究为进一步探索EgDWF5基因调控油棕中油体的形成机制奠定基础。     

Properties of poorly digestible fraction of protein bodies of cooked milled rice

Pepsin-treated protein bodies and human fecal protein particles from IR480-5-9 cooked milled rice (Oryza sativa L.) consisted mainly of giutelin-type polypeptides with a mean molecular weight (MW) of 16 000. They had similar MW, amino acid pattern, and isoelectric focusing characteristics as the 70% 2-propanol—0.6% -mercaptoethanol soluble fraction of rice glutelin. The lipids of the pepsin-treated protein bodies had an identical lipid fraction ratio and fatty acid composition as the lipids of whole protein bodies.     

陕西省咸阳市涝池污染特征和水质现状

[目的] 针对涝池近年来出现的水体富营养化现状,明确涝池污染特征和水质现状,为涝池水体净化研究和水环境保护提供科学依据。[方法] 选取陕西省咸阳市14个具有代表性的涝池,从涝池的面积、深度、蓄水状况、主体功能等方面出发,测定其水体PH,DO,TN,NH₄⁺-N,NO₃^--N,TP,PO₄^3--P的浓度,应用标准指数法和综合污染指数法综合分析涝池水质状况。[结果] ①调查区域的涝池水体污染与涝池管护缺失导致的外源污染严重和乡村居民农业生产活动造成的农业面源污染有关。②大部分涝池水质处于严重污染状态,无法满足农业用水及一般景观的用水标准,其中,磷类营养盐是水体污染的主要因子。[结论] 涝池水体污染具有农村生活污水的典型特点,应根据水体污染特点有针对性地选择适宜乡村环境的治理方式。     

半夏类原球茎的诱导及其植株再生研究

研究不同外植体和植物激素对半夏类原球茎诱导的影响,并在此基础上建立半夏高频再生体系。以贵州大方‘圆珠’半夏为材料,进行了半夏类原球茎的诱导、分化和驯化移栽技术的试验研究。块茎诱导类原球茎的适宜培养基为MS+6-BA 1.0 mg/L+NAA 0.3 mg/L,诱导率和增殖倍数分别为86.67%和17.40。叶片和叶柄则采用MS+6-BA 1.0 mg/L+NAA 0.1 mg/L配比的培养基效果较好,类原球茎诱导率均可达50%以上,最大增殖倍数分别为12.80和8.6。炼苗移栽时,选用直径为0.5 cm、根数为9.4个/株、根长为3.71 cm 左右的组培苗,移栽成活率高且植株生长良好。组培苗移栽基质为园土和珍珠岩体积比1：1最好。在适宜的植物激素配比下,可从合适的半夏外植体上诱导出类原球茎,建立半夏高频再生体系,为半夏的种质资源保存、基因工程育种和药用次生代谢产物的生产等奠定基础。     

Isolation and characterization of oil bodies from Oryza sativa bran and studies of their physical properties

In this paper, we demonstrate a novel and environmentally-conscious approach to the isolation of oil bodies (OBs) from the bran arising from the milling of Oryza sativa (Basmati rice). We have used several physical techniques to determine the effect of the steps of the process on the OBs, and describe an isolation process that is scalable to an industrial level. The physical techniques [microscopy, particle size determination (diameter 1.9–5.8 μm), ζ-potential (−40 mV at pH 8.0, 0 mV at pH 4.0, 17 mV at pH 2.0), and relative turbidity measurements (pH 3.0–5.0 unstable, pH 6.0–8.0 stable)] and chemical analyses (lipid 83.7%, protein 11.5% dry basis) also give us an insight into the physical properties of OBs in general. This understanding has implications for the use of OBs in food manufacturing, and on the isolation of OBs from a variety of cereal crops.     

农杆菌介导的DnMADS2基因转化金钗石斛初步研究

为建立稳定有效的金钗石斛转化体系,进一步研究花发育相关基因的功能,为石斛兰分子育种提供基础,本研究以金钗石斛类原球茎（PLBs）为转化受体,通过农杆菌介导的方法将DnMADS2基因转入金钗石斛。结果表明：用20 mg/L潮霉素筛选4个月后获得抗性PLBs,对其进行HPT基因和DnMADS2基因的PCR检测表明,目的基因已成功整合到PLBs中。获得的转化PLBs在抗性培养基上分化为不定芽后,再经生根壮苗培养获得转化植株。对转化植株进行Southern检测,结果表明,DnMADS2基因已整合到5株金钗石斛转化植株基因组中。据此建立的稳定高效的金钗石斛转化体系和获得的转基因植株,将为DnMADS2基因功能的进一步研究和金钗石斛转基因育种奠定基础。     

水稻秸秆中硅体的光催化活性研究

采用干燥-灼烧-煅烧工艺从水稻秸秆中制备硅体,并对硅体进行表征和光催化活性研究。结果表明,制备的硅体由无定形的微米、纳米二氧化硅颗粒组成,并具有光驱类脱氢酶活性和光催化降解甲基紫的能力。在模拟的光源下,在人工模拟的光源下,硅体催化红四氮唑(TTC)迅速转变成不溶于水的三苯甲簪(TPF),硅体的光驱类脱氢酶活力值达到2622μg TPF ·g-1 ·min-1;光催化降解甲基紫染料的结果表明硅体对甲基紫的去除率2 h内达到94.4%。秸秆中硅体的光催化特性为生物质资源的开发利用提供新途径,同时也对自然界中生物材料结构与功能的研究提供了新的思路。