食品微生物检测中PCR技术的创新应用与研究

PCR技术在食品检测的实际应用中表现出速度快、简便、高效等特点,为食品检测技术的发展提供了有力的技术支持,成为调查食品源疾病暴发及鉴定相应病原菌的有效工具。因此,我们应当加大科研力度,组建由各种层次专业人员构成的技术队伍,从而进一步完善食品检测科研管理体系。     

Development and validation of a tertiary simulation model for predicting the growth of the food microorganisms under dynamic and static temperature conditions

The use of predictive modeling software may markedly contribute to the better understanding of the microbial behavior in foods. In this paper, the development and validation of a tertiary model, which provides predictions of microbial growth in foods under dynamic or static temperature conditions, is presented. In particular, the UGPM (Unified Growth Prediction Model) software applies the Baranyi and Roberts (1994) primary model, coupled to a secondary temperature model, in order to simulate growth of a given microorganism during storage of a specific food or food category. The software, intended to be used by both expert and non-expert users, may be a valuable decision support tool for the food industry, by assisting in the management of foods based on their actual shelf-life and microbial safety, thereby limiting the deterministic “best-by” practice for the determination of shelf-life. The latter is commonly based solely on empirical observations and has high uncertainty. This in turn, may result in the rejection of large quantities of unspoiled or safe foods, or even in the distribution of spoiled and unsafe foods, due to ignorance of the effect of temperature abuse on the microbial spoilage and safety of products.     

Three level direct torque predictive controlbased on fast virtual vector modulation

A novel scheme based on voltage prediction and fast virtual vector modulation algorithm for direct torque control of a permanent magnet synchronous motor fed by diode clamped multi level inverter is proposed.The system is based on diode clamped three level inverter,which is widely applied to middle high voltage speed regulation.Combined with space vector modulation,the reference voltage vector for direct torque control is obtained by voltage predictive control algorithm,then zero vector and two adjacent virtual vectors are used to synthesize reference voltage vector based on non orthogonal 60° coordinate.The neutral point potential is controlled by using the neutral point current characteristics of small vectors,the desired basic voltages and their dwelling time can be easily obtained and fast space vector modulation is achieved.Simulation results verify the feasibility of the proposed control scheme,that dv/dt of output voltage and neutral point potential of three level inverter are restrained effectively,and high dynamic and steady performances are obtained.     

Reference values of respiratory and peripheral muscle function in rats

Skeletal muscle dysfunction is a common systemic manifestation in several prevalent diseases. Predictive values are useful tools for the diagnosis and prognosis of diseases. In experimental animals, no reference values of muscle function evaluation have been so far reported. The objective was to obtain predictive values of maximal inspiratory pressure (MIP) and grip strength measurements in healthy rats. In 70 healthy rats, MIP and grip strength were measured in vivo weekly for five consecutive weeks using non‐invasive methodologies. Three ranges of rat body weights (250–299, 300–349 and 350–399 g) and lengths (37.0–41.0, 41.1–42.0 and 42.1–44.0 cm) were established. MIP and grip strength measurements falling within the ranges of weight 350–399 and 300–349 g and length 42.1–44.0 cm were significantly greater than values falling within 250–299 g and 37.0–41.0 cm ranges respectively. Specific weight‐ and length‐percentile distributions for MIP and grip strength measurements were calculated. As significant direct correlations were observed between rat weights and lengths and either MIP or grip strength measurements, regression equations relating all these variables were also determined. Skeletal muscle dysfunction is frequently associated with highly prevalent conditions. The significant predictive equations described for both MIP and grip strength measurements will enable scientists to better estimate the respiratory and peripheral muscle dysfunctions of laboratory animals, especially when conducting follow‐up and/or intervention investigations.     

Enhancement of Nodulation and Yield of Chickpea by Co-inoculation of Indigenous Mesorhizobium spp. and Plant Growth–Promoting Rhizobacteria in Eastern Uttar Pradesh

We study the effect of plant growth–promoting rhizobacteria (PGPR) along with Mesorhizobium sp. BHURC02 on nodulation, plant growth, yield, and nutrient content of chickpea (Cicer arietinum L.) under field conditions. A similar study has been conducted for nodulation and plant growth of chickpea in pot experiment under glasshouse conditions. The treatment combination of Mesorhizobium sp. BHURC02 and Pseudomonas fluorescens BHUPSB06 statistically significantly increased nodule number plant^–1, dry weight of nodule plant^–1, and root and shoot dry weights plant^–1 over the control under a glasshouse experiment. The maximum significant increase in nodule number, dry matter, and nutrient content were recorded in co-inoculation of Mesorhizobium sp. BHURC02 and P. fluorescens BHUPSB06 followed by co-inoculation of Mesorhizobium sp., Azotobacter chroococcum, and Bacillus megatrium BHUPSB14 over uninoculated control in a 2-year field study. Hence, co-inoculation of Mesorhizobium sp. and P. fluorescens may be effective indigenous PGPR for chickpea production.     

信息技术与食品微生物学的整合研究与实践

本文在总结多次开设农业和食品微生物学教学实践尝试，探讨在新的教育思想与教学理论的指导下，应用信息技术优势与农业和食品微生物学课程有效整合的新型教学模式、旨在提高教学效果的有益探索。     

食品微生物实验教学改革研究

从优化教学内容、更新教学手段、改革考核方式三个方面对广东海洋大学食品微生物学实验教学改革进行探究,使得该课程更加适合广东海洋大学食品学院教学目标,提高了学生的动手与创新能力及课程教学质量.     

Nonlinear Predictive Control of Small Sag cable with Axial Displacement

An approach for the control of a small sag cable is presented.The approach is based on a nonlinear predictive control scheme that determines the required axial displacement input so that the predicted response match the desired Results.It is accomplished by minimizing the norm squared local errors between the predicted and desired values.Finally simulation results that use the new control strategy to demonstrate the effective are show.     

民族学生《兽医微生物学》综合性实验教学模式改革探索与实践

以培养应用型人才为目标,结合民族学生的特点,改革综合性实验教学模式,激发民族学生对兽医微生物学实验兴趣,培养民族学生独立思考、动手能力。