番茄育苗播种机双腔排种滚筒的性能试验研究

针对现有气吸滚筒式排种器部件多、装配难、密封性差、无正压投种气室,以及难于实现正压投种清堵功能、直接造成排种器漏播率高的的技术问题,提出了利用3D快速成型技术打印剖分式负压吸种滚筒的新思路,并创新设计了双腔排种滚筒。以番茄种子为试验对象,以滚筒转速、滚筒负压和投种腔气室正压为试验因素,进行正交回归试验,并利用Design-Expert软件对试验数据进行方差分析和回归拟合,分析了影响排种性能指标的滚筒负压、滚筒转速和投种腔气室正压的显著性,确定了影响排种性能指标较优的结构参数、工作参数,即当投种腔气室正压为2.2k Pa、滚筒负压为2.4k Pa、滚筒转速为16r/min时,性能指标的最优值及为单粒率93.2%,重播率4.3%,漏播率2.5%,均满足精量排种的技术要求。     

日光温室正压湿帘冷风降温性能及冷负荷计算模型

负压湿帘风机降温被广泛应用于温室生产中,但存在降温均匀性差、限制温室长度及对温室密闭性要求高等不足。为克服负压湿帘风机降温的局限性,提高日光温室降温能力,该研究设计了日光温室正压湿帘冷风降温系统,其气流组织方式为湿冷空气从南屋面底部进入日光温室,热空气由顶开窗排出室外。在北京地区无作物的日光温室对系统夏季降温增湿效果及性能进行试验,试验结果表明:在典型夏季高温白天,正压湿帘冷风降温系统配合遮阳网可将日光温室试验区内平均气温控制在30.7～33.4℃,比采用自然通风配合遮阳网的对照区低5.4～11.1℃,比室外低2.4～5.4℃,降温效果良好;夜间系统对温室降温幅度减小。该系统可有效缓解低湿胁迫,日光温室试验区空气平均相对湿度为49.8%～62.3%,比对照区及室外分别高13.6%～21.2%和13.6%～24.6%。室内风速0.35～1 m/s,气流分布差异性较小。试验条件下,正压湿帘冷风降温系统的平均降温效率为91%,比传统的负压湿帘风机高10个百分点以上;实际平均耗水量为0.035～0.079 g/(m~2·s),且耗水量与室外空气水蒸气饱和压差(VPD,vapor pressure deficit)呈正相关(P0.01,r=0.64)。同时,研究构建了日光温室冷负荷计算模型及湿帘冷风降温设备合理选型方法,其中冷负荷模型是降温设备选型的基础,普遍适用于各种日光温室降温方法的研究。计算得到日光温室夏季降温冷负荷为299.1W/m~2,应安装的正压湿帘冷风降温系统最大比通风量为0.067 m/s。该研究为日光温室正压湿帘冷风降温方法的工程应用提供了技术参考,为日光温室安全越夏生产环境控制提供了理论基础。     

专用堆型对应的核主泵正反转流动数值模拟

为了研究核主泵在定转速工况下的正反转特性,采用相似换算法,基于SST k-ω 湍流模型与块结构化网格,对缩比系数为0.5 的核主泵模型泵进行数值模拟.定义流量从泵进口流向出口为“+”,反之为“-”.在正转工况下分别对-0.8Q_d到+2.0Q_d流量范围内的16个工况点进行计算、反转工况下对-1.4Q_d到+1.0Q_d流量范围内的14个工况点进行计算,得到其全特性曲线.计算结果表明:在相同流量工况下,核主泵正转时的扬程与转矩总是高于反转时的扬程与转矩,叶轮扬程与泵扬程存在不同的变化趋势;在正转工况下,在 -0.1Q_d到+0.4Q_d流量范围内,叶轮扬程曲线呈现反“N”型变化趋势;在反转工况下,在-0.4Q_d到+0.1Q_d流量范围内,叶轮扬程曲线呈一个明显的“V”型变化趋势;叶轮出口处产生二次流回流现象,这是正转小流量工况下叶轮扬程降低的主要原因,而叶轮与导叶之间过渡段区域内的环形高速带和叶轮流道内的大尺度涡是反转小流量工况下叶轮扬程降低的主要原因.     

过量表达OsNPR1基因稳定提高水稻对白叶枯病的抗性

在拟南芥中,NPR1是系统获得抗性SA信号传导途径中的一个重要的调节因子,在水稻中已克隆到与之同源的OsNPR1基因。构建OsNPR1基因水稻过量表达载体,并将其转化粳稻TP309得到转基因植株;通过自交纯合,得到17个纯合株系;对T3、T4代纯合株系进行PCR鉴定,证实转基因纯合株系中外源伪OsNPR1基因具有遗传稳定性;检测了T1、T2代转基因株系和T3代转基因纯合株系对水稻白叶枯病病原细菌Xanthomonas oryzae pv．oryzae的抗病性,结果表明,在T1、T2代中70％以上的株系对水稻白叶枯病的抗性显著提高,T3代中约67％的株系对水稻白叶枯病的抗性显著提高,说明这种抗病性的提高具有遗传稳定性。OsNPR1基因可作为选育水稻抗白叶枯病新种质的一个良好的候选基因。     

空气罐与超压泄压阀联合水锤防护特性

提出了一种空气罐与超压泄压阀联合设置的停泵水锤防护方案,并基于瞬变流计算的特征线法建立了空气罐、液控蝶阀、超压泄压阀等边界条件的数学模型,模拟了停泵工况下系统的压力变化过程.结合工程实例,对比分析了空气罐单独防护、空气罐与两阶段关闭泵后液控蝶阀联合防护以及空气罐与超压泄压阀联合防护对停泵水锤的影响.在空气罐体型一定时,对超压泄压阀的启闭规律进行了敏感性分析.计算结果表明,空气罐与两阶段关闭泵后液控蝶阀联合防护方案对输水系统正负水锤防护均不利;而空气罐与超压泄压阀联合防护方案对输水系统正负水锤均有较好的防护效果,与空气罐单独防护方案相比,泵后高压管段最高压应力由1.343 MPa降至1.087 MPa,在满足承压标准1.2 MPa的基础上安全裕度提高了9.4%,空气罐体型也由200 m³缩减至160 m³.超压泄压阀应在5 s内开启至全开度,且开启后持续时间应接近1个相长.     

Adaptive evolutionary expansion of the ribonuclease 6 in Rodentia

Ribonuclease 6 (RNase6 or RNase K6) is a protein that belongs to a superfamily thought to be the sole verte‐brate‐specific enzyme known for a wide range of physiological functions, including digestion, cytotoxicity, angiogenesis, male reproduction and host defense. In our study, 51 functional genes and 11 pseudogenes were identified from 27 Rodentia species. Intriguingly, in the 3 main lineages of rodents there were multiple RNase6s identified in all species of Ctenohystrica, whereas only a single RNase6 was observed in other Rodentia species examined except for 2 species in the mouse‐related clade. The evolutionary scenario of “birth (gene duplication) and death (gene deactivation)” and gene sorting have been demonstrated in Ctenohystrica. In addition, bursts of positive selection, diversification of isoelectric point and positive net charge have been identified in Ctenohystrica, especially at two key sites that are involved in antimicrobial function. Site Trp30 has undergone positive selection and Ile45 has changed into other residues in Group B and Group C of the Ctenohystrica. Our results demonstrated a complex and intriguing evolutionary pattern of rodent RNase6, and indicated that functional modification may have occurred, which establishes an important theoretical foundation for future functional assays in rodent RNase6.     

Congenital orbital cysts of neural tissue in two dogs

A 3-month-old English Cocker Spaniel and a 6-month-old miniature poodle presented with clinical signs related to an abnormal right eye since birth. In both dogs, the right globe could not be identified and was replaced by a fluctuant intraorbital mass covered by a vascularized mucous membrane. Ultrasonography demonstrated that both masses were cystic structures markedly larger in size than the normal contralateral globes. In both cases, surgical excision revealed a multilobular cyst filling the whole orbital cavity. Histopathologic examination and immunostaining for glial fibrillary acid protein and S100 protein supported a diagnosis of neural cysts associated with ocular dysplasia. The definitive diagnosis was congenital cystic eye and microphthalmos with cyst for the Cocker Spaniel and miniature poodle, respectively. Karyotype was normal in both dogs. Congenital cystic eye and microphthalmos with cyst result from defects in early embryonic life that arise following formation of the optic vesicle and prior to closure of the optic fissure. To the authors' knowledge neither has been reported in the canine species. They should be considered in the differential diagnosis of orbital cysts in dogs.     

冀西北地区绵羊螨虫感染情况调查研究

为摸清冀西北地区绵羊螨虫的感染情况,选取冀西北地区坝上和坝下几个具有代表性的养殖场,通过临床症状、显微镜检查和统计学分析对绵羊感染螨虫的发病情况、不同年龄、不同季节感染情况进行试验研究。结果表明:冀西北地区绵羊螨虫阳性率44%,不同年龄、不同季节的绵羊螨虫阳性率均具有显著性差异(P<0.05)。     

Whole genome sequence analysis to detect signatures of positive selection for high fecundity in sheep

Ovulation rate and prolificacy are the most important reproductive traits that have major impact on the efficiency of lamb meat production. Here, we compared the whole genomes of the Romanov sheep, known as one of the high prolific breeds, and four other sheep breeds namely Assaf, Awassi, Cambridge and British du cher, to identify genetic mechanisms underlying prolificacy in sheep. Selection signature analysis revealed 637 and 477 protein‐coding genes under positive selection from F_ST and nucleotide diversity (Pi) statistics, respectively. Further analysis showed that several candidate genes including LEPR, PDGFRL and KLF5 genes are involved in sheep prolificacy. The identified candidate genes in the selected regions are novel and provide new insights into the genetic mechanisms underlying prolificacy in sheep and can be useful in sheep breeding programmes to develop improved breeds for high reproductive efficiency.     

黄土丘陵区植物间正相互作用对幼苗更新的影响

幼苗阶段是植物生活史中最脆弱与关键的阶段,在土壤侵蚀强烈的黄土坡面,现有优势物种能否作为保育物种发挥植物间正相互作用促进幼苗更新与存活还缺乏研究。以黄土丘陵区的3种典型植物群落(白羊草群落、狼牙刺群落、铁杆蒿群落)为研究对象,采用样方调查法研究了植物斑块和裸地斑块下幼苗的密度、物种组成、物种多样性以及与地上植物物种相似性的变化规律,对不同植物间的正相互作用的强度进行分析。结果表明:1)在研究区共记录到幼苗 52种,分属22科,主要由禾本科、豆科和菊科植物构成,且以多年生草本植物为主;2)不同斑块下幼苗的物种数量、密度、多样性和相似性指数大体表现为植物斑块>裸地斑块;同为植物斑块,不同植物群落幼苗物种数量是铁杆蒿群落>白羊草群落>狼牙刺群落,密度为铁杆蒿群落>狼牙刺群落>白羊草群落,多样性指数为铁杆蒿群落>白羊草群落>狼牙刺群落;而裸地斑块的幼苗密度与多样性指数均为铁杆蒿群落>狼牙刺群落>白羊草群落。3种群落两种斑块幼苗与地上植物的相似性系数均低于0.5,属于极不相似或中等不相似水平;3)植物斑块中的成年植株对其周围的幼苗存在积极的正相互作用,但在侵蚀干扰更加强烈,环境更加恶劣的阳坡白羊草群落、狼牙刺群落不同斑块间物种组成差异更大,植物改善微生境,促进更多类型幼苗更新的作用更加明显。总之,3种植物作为保育植物均能促进幼苗存活,但随着植株个体生长,可能存在物种间相互作用的转变,使得幼苗和地上植物的相似性不高。