悬浮培养在口蹄疫疫苗中的应用

通过生物反应器中进行BHK21细胞悬浮培养并逐级放大,分别接种口蹄疫OJMS/2000株与Asia 1/JSL株,纯化灭活后制备50批疫苗,结果均符合《口蹄疫O型、亚洲I型二价疫苗（OJMS株＋JSL株）制造及检验规程》（以下称规程）所规定的各项标准,病毒146S抗原含量比转瓶培养提高10倍以上、疫苗的不良反应得到进一步改善。     

塔落岩黄芪、细枝岩黄芪及其变异型的生理特性研究

本文研究了毛乌素沙漠地区塔落岩黄芪和细枝岩黄芪及其变异型的生理学特性。结果表明,塔落岩黄芪和细枝岩黄芪各与其变异型之间在生理特性方面存在着极其显著的差异。     

科尔沁草地的畜草平衡与草业发展途径的研究

根据实地调查和历年的统计资料，系统分析研究了科尔沁草地多年来草业发展速度落后于牲畜增长速度、畜草供需严重失衡的现状。结果表明，草地退化４５．９％，超载率达３６．１％。该地区发展草业的自然和社会经济条件优越，但必须采取以下措施才能达到畜草平衡的目的：加强合理利用和改良天然草地；发展人工饲草料基地，迅速增加种植畜牧业比例；大力发展秸秆及农副产品饲草料加工业     

副结核分枝杆菌DNA探针的制备与应用

以溶菌酶、ＳＤＳ、高氯酸钠等处理提取副结核分析杆菌Ｃ２株染色体ＤＮＡ，经限制性内切酶Ｐｓｔ Ｉ消化后，以质粒ｐＢｌｕｅｓｃｒｉｐｔ ＳＫ为载体，通过Ｔ４ＤＮＡ连接酶连接，转入Ｅ。ｃｏｌｉ ＤＨ５ａ受体菌中，构建了副结核菌Ｃ２株的ＤＮＡ基因文库。应用反向杂交试验，从基因文库中筛选出４个重组克隆：ＰＴＰ１２、ＰＴＰ１９、ＰＴＰ３８。对这４个重组克隆进行酶切、电泳分析，结果表明４个插入片段长度分别为：４     

柔毛野豌豆根瘤菌接种效果试验研究

对柔毛野豌豆进行了根瘤菌接种,使柔毛野豌豆的产草量提高了６０％－６９％,土壤多固氮３０ｋｇ／ｈｍ＾２,改良了土壤,增加了绿肥效果,为促进生态农业和生态畜牧业的有机结合开辟了新途径。     

基于GIS的澳洲坚果在云南适宜种植区划分研究

为促进云南省澳洲坚果产业持续发展,利用GIS技术将7个气候要素和DEM网格图叠加分析,划分澳洲坚果在云南种植的最适宜、次适宜和不适宜区。结果表明,最适宜区面积57 939 km²,占云南省国土面积的14.7%;次适宜区面积63 977 km²,占云南省国土面积的16.2%。澳洲坚果可种植区域为文山州、红河州、普洱市、德宏州、临沧市、保山市、西双版纳州及玉溪南部地区,主要为云南热带、亚热带地区的河谷地带、低海拔山地区域。     

猪霉麦中毒和霉麦无害化处理的研究

对1983年嫩江地区某地猪喂霉麦引起的中毒进行了系统研究。通过霉菌菌相、菌量分析,明确了霉麦的优势菌为黄曲霉菌;通过流行病学调查、优势菌的产毒试验、毒素的化学确证试验、当日鸭雏的生物学确证试验以及猪的回归试验等,证明黄曲霉毒素B_1(AFT B_1)是造成猪短期内大批死亡的原因。同时,进行了霉麦无害化处理试验,初步证明用0.02％次氯酸钠处理具有杀菌、除毒效果可靠等特点。     

伊克昭盟牧草和饲料作物应用增产菌技术的试验

199年伊克昭盟引入了牧草和饲料作物增产菌这一技术,经过7年的试验,示范及推广,至今全盟该增产菌应用面积已达4．04万hm^2,本文通过7年来在两种不同生境下,对12种牧草及作物的不同物修期采用不同方法施用增产菌,以鲜草产量和种子产量为衡量标准来探讨增产菌对不同牧草及作物的增产效果,实验结果表明：增产菌技术的应用,促进了植物的生长,使植株长高,叶面积增大,加强了光合作用,在提高鲜草产量的同时也增加了籽实的产量,具有较强的推广价值,是一项在农牧区适用的增产技术。     

湟中马血清酯酶多态性的研究

采用聚丙烯酰胺凝胶电泳法对青海省湟中县９１匹马血清酯酶的多态性进行了研究。结果发现：①湟中马的血清酯酶受ＥＳ＾Ｆ，ＥＳ＾Ｉ，ＥＳ＾Ｓ和ＥＳ＾Ｏ４个等位基因的控制，其基因频率分别为０．４４５０，０．５３３０，０．０１１０和０．０１１０；②在被检湟中马的ＥＳ位点上共发现ＥＳ Ｆ，ＥＳ ＦＩ，ＥＳ Ｉ，ＥＳ ＩＳ和ＥＳ Ｏ五种表型，以ＥＳ ＦＩ为优势表型（４７．２５％）；③ＥＳ等位基因频率无性别差异；④     

Effects of vegetable oil supplementation on feed intake,rumen fermentation,growth performance,and carcass characteristic of growing swamp buffaloes

Fifteen, one year old swamp buffalo males with average liveweight of 200.5 ± 9.5 kg were randomly assigned according to a completely randomized design to receive three dietary treatments of supplemental vegetable oils in concentrate contained 140, 750, and 16 g of crude protein, total digestible nutrient, and ether extract, respectively (T1 = unsupplemented, T2 = supplemental coconut oil and sunflower oil in ratio 50:50 at 6% of concentrate, and T3 = supplemental sunflower oil at 6% of concentrate) during a 5-month feeding trial. Urea-treated rice straw was offered ad libitum and concentrate was supplemented at 150 g/kg BW. The results revealed that supplementation of vegetable oils decreased dry matter intake in sunflower oil supplemented group. Combination of coconut oil and sunflower oil supplementation resulted in similar average daily gain as those found in control and in sunflower oil supplemented groups. Rumen microorganism population, ammonia–nitrogen, blood urea–nitrogen and total volatile fatty acid concentrations were decreased as vegetable oils were supplemented. Oil supplementation slightly reduced growth rate, hot carcass percentage, loin eye area particularly on sunflower oil supplemented group. Based on these findings, vegetable oil resulted in decreased performance of swamp buffaloes although the combination of coconut oil and sunflower oil could reduce negative effect of unsaturated oil supplementation. However, further analyses of conjugated fatty acids (CLA) in meat could offer useful information in determining pronounced effects of vegetable oil supplementation.