果树栽植机光电定株距装置的设计与试验

针对果树栽植过程中株距难以控制的问题,考虑到现有的果树栽植机定株距存在结构复杂、制造成本高及受土壤滑移率影响较大等问题,设计了一种光电定株距装置,可以避免土壤滑移率对株距的影响,保证栽植果树株距的一致性,且可实现株距连续可调,满足不同果树栽植时对株距选择的要求。针对试验中出现的部分株距较设计值有所偏差的问题,分析了原因并提出了优化方案。试验结果表明:果树栽植机光电定株距装置栽植合格率92.5%,平均栽植深度合格率为92.9%,在1.7m栽植株距下,株距变异系数为5.9%,满足现代标准果园机械化生产要求。     

Chromosomal regions controlling anther culturability in rice (Oryza sativa L.)

Diallel analysis has revealed that anther culturability in rice (Oryza sativa L.) is a quantitative trait controlled by the nuclear genome. Mapping of anther culturability is important to increase the efficiency for green plant regeneration from microspores. In the previous study, we detected distorted segregation of RFLP markers in rice populations derived from the anther culture of an F₁ hybrid between a japonica cultivar ‘Nipponbare’ and an indica cultivar ‘Milyang 23’. To clarify the association between chromosomal regions showing distorted segregation and anther culturability, the anther culturability of doubled haploid lines derived from the same cross combination was examined, and the association between alleles of the RFLP markers, which exhibiting the most distorted segregation on chromosomes 1, 3, 7, 10 and 11, and the anther culturability was evaluated. One region on chromosome 1 was found to control callus formation from microspores, and one region on chromosome 10 appeared to control the ratio of green to albino regenerated plants. In both regions, the Nipponbare allele had positive effects. Three regions on chromosomes 3, 7 and 11, however, showed no significant effect on anther culturability. This revised version was published online in August 2006 with corrections to the Cover Date.     

乙醛脱氢酶基因（ALDH）转化番茄的研究

乙醛脱氢酶基因（ALDH）为目的基因,构建了pBI-ALDH 植物表达载体,番茄品系03HN31子叶为外植体,经发根农杆菌（Agrobacterium rhizogens）介导采用共培养法转化番茄。通过分子生物学技术（PCR、Southern杂交、RT-PCR）和抗逆相关生理指标测定（相对电导率及丙二醛含量）相结合的方法对转化番茄植株进行检测,研究结果表明：乙醛脱氢酶基因（ ALDH ）导入并整合到番茄的基因组中,在转录水平上可以稳定表达;在干旱、高盐和低温胁迫条件下,转基因番茄植株和对照株的相对电导率和丙二醛含量有差异,证明转化番茄植株的质膜受损程度有所降低,抗氧化胁迫性能有所提高,转化率为10.78%。     

江苏省天福国家湿地公园鸟类栖息地植物群落特征对鸻鹬类水鸟的影响研究

目的 研究栖息地植物群落特征的季度变化规律,探讨其对鸻鹬类水鸟群落的影响机制,为鸟类栖息地管理提供科学依据。 方法 2019/07—2020/07期间,在天福国家湿地公园鸟类栖息地,利用样方法和样线法分别获取植物群落特征数据和鸻鹬类水鸟群落数据,通过转换变量,构建回归模型。通过主成分分析和聚类分析对鸻鹬类水鸟进行鸟类集团划分。 结果 统计分析发现：（1）天福国家湿地公园共记录到草本植物30科72属77种,鸻鹬类水鸟18种230只;（2）植物群落高度和植被覆盖度的比值与鸻鹬类水鸟个体总数和鸟类个体总数的比值呈显著正相关;（3）不同性状的鸻鹬类对植物群落特征的季度变化响应程度有差异,通过主成分分析和聚类分析将鸻鹬类水鸟划分成4个鸟类集团,其中集团4即中翼长、中嘴裂长、中跗跖长的鸻鹬类水鸟受植物群落特征的季度变化影响大于其他鸻鹬类鸟类集团。（4）目前天福国家湿地公园保护管理中心未进行区分不同植物群落而采取有差别的管理措施,属于无区别化干扰。 结论 天福国家湿地公园鸟类栖息地植物群落特征对鸻鹬类水鸟群落特征有显著影响,且对不同鸻鹬类鸟类集团的影响机制具有差异性;研究区无区别化的栖息地管理措施须结合植被群落演替规律及不同性状鸻鹬类水鸟的生境需求而精准改进,建议针对不同鸻鹬类鸟类集团的差异性生境需求,精准管理不同的植物群落特征,同时增加不同类别的栖息地管理措施。     

Analysis and comparison on population dynamics betweenAdenophora lobophylla andAdenophora potaninii by leslie matrix

The population dynamic tendency ofAdenophora lobophylla Hong as an endangered species andAdenophora potaninii Korsh as widespread species, has been predicted by the Leslie matrix. And the comparison and analysis on the age structures between two species have been carried out in this paper. The results demonstrate theA. lobophylla populations which have the reasonable age structures perform slowly negative or positive increment at altitude 2300–3400 m. Especially. below altitude 2700 m. there are many populations performing seriously declining tendency. Contrary.A potaninii populations could adapt to environment perfectly at the corresponding condition without finding the population which performs the seriously declining tendency. The differences in developing tendency of population between the two species demonstrate thatA. lobophylla populations have the weaker ability to adapt to the external unfavorable conditions. The project was supported by Chinese Natural Science Foundation. (No. 3939150) The experiment carried out at the Botanical Department of University Federal Sao Carols in Brazil (Responsible Editor: Zhu Hong)     

Suppression subtractive hybridization library construction and identification of epidermal bladder cell related genes in the common ice plant,Mesembryanthemum crystallinum L.

Mesembryanthemum crystallinum L., a halophytic species, displays modified trichomes, epidermal bladder cells (EBC), on the surfaces of its aerial organs. EBCs serve to sequester excessive salt from underlying metabolically active tissues. To elucidate the molecular determinants governing EBC development in the common ice plant, we constructed a cDNA-based suppression subtractive hybridization library and identified genes differentially expressed between the wild-type and the EBC-less mutant. After hybridization, 38 clones were obtained. Among them, 24 clones had homology with plant genes of known functions, whose roles might not be directly related to EBC-morphology, while 14 clones were homologous to genes of unknown functions. After confirmation by northern blot analysis, 12 out of 14 clones of unknown functions were chosen for semi-quantitative RT-PCR analysis, and the results revealed that three clones designated as MW3, MW21, and MW31 preferentially expressed in the EBC-less mutant, whereas the other two designated as WM10 and WM28 preferentially expressed in the wild type. Among these genes, the expression of a putative jasmonate-induced gene, designated as WM28 was completely suppressed in the EBC-mutant. In addition, the deletion of C-box cis-acting element was found in the promoter region of WM28 in the EBC-less mutant. Overexpression of WM28 in Arabidopsis resulted in increased trichome number due to the upregulation of key trichome-related genes GLABRA1 (GL1), and GLABRA3 (GL3). These results demonstrate that WM28 can be an important factor responsible for EBC formation, and also suggest the similarity of developmental mechanism between trichome in Arabidopsis and EBC in common ice plant.     

植物生长调节剂对香梨采前落果、果实品质及产量的影响

以库尔勒香梨果树为试材,在香梨幼果膨大期喷施不同浓度生长植物调节剂,每隔15 d喷施1次,共喷施5次,收果时调查香梨的坐果率、果实品质及产量,并分析经济效益。结果表明:以NAA 10 mg、GA 10 mg及黄腐酸复合微量元素的处理效果较为明显,该处理降低了香梨采前落果率,提高了香梨一级果率,并改善了果实品质,增加了种植效益。     

种瓣及密度对大蒜产量的影响

采用二次正交通用旋转设计，进行了种辩大小与种植密度对大蒜产量影响的研究，建立了大蒜产量与种植密度及种瓣大小间的二元二次数学模型。通过模型解析得出，种植密度对产量的影响大于种瓣大小，但密度对单株产量的贡献为负值。通过计算机模拟试验，得出本试验条件下，获得３００００ｋｇ／ｈｍ２以上产量的种植密度为５２．９５～６３．４５万株／ｈｍ２、种瓣大小为４．９～７．０ｇ;;单株产量大于５０ｇ的种植密度为３４．０５～４．５５万株／ｈｍ２、种瓣大小４．９～７．０ｇ。     

植物杂种优势利用的一条新途径—细胞核+细胞质雄性不育(GCMS)

本文在综述了植物细胞核雄性不育和细胞质雄性不育优点和缺点基础上提出了植物杂种优势利用的一条新途径——细胞核十细胞质雄性不育,根据细胞核不育基因的显隐性、稳定性和细胞质雄性不育的稳定性不同,可以将该途径进一步分成显性细胞核+细胞质雄性不育、隐性细胞核+细胞质雄性不育、细胞核不育+生态型、细胞质雄性不育和细胞质不育+生态型细胞核雄性不育。     

放牧强度对草地植物组成及植物生物量的影响

简述了放牧对草地植物组成及植物生物量的影响 ,提出从保护草地植物多样性及获得较高草地生产量的角度出发 ,应使草地承受适当的放牧压力