Multifocal cutaneous histiocytic sarcoma in a young dog and review of histiocytic cell immunophenotyping

A 9‐month‐old male Great Dane had progressive generalized nodular dermatopathy for several months. There were > 100 raised, alopecic, firm, painful nodules throughout the skin. Aspirates from several lesions yielded moderate numbers of irregularly round or polygonal to spindle‐shaped cells with mild to moderate anisocytosis and few inflammatory cells, and the cytologic interpretation was proliferation of mesenchymal or histiocytic cells. On histopathologic examination, nodules were composed of densely packed sheets of round to spindle‐shaped cells with mild anisokaryosis and low mitotic activity. Multifocal histiocytic sarcoma with a spindle‐cell pattern was diagnosed based on morphologic features and intense expression of CD18. Additional immunophenotypic analysis on frozen sections of tissue confirmed the diagnosis of histiocytic sarcoma; expression of CD18, CD45, CD1a, CD11b, and CD11c, limited expression of Thy‐1 (CD90) and CD80, and lack of expression of CD4, CD11d, and CD86 indicated that the cells were likely interstitial dendritic cells; a review of reactive and neoplastic dendritic cells is provided. Based on staging, internal organs were not affected. Sequential treatment with lomustine and doxorubicin failed to prevent progression of the cutaneous lesions, and the dog died 3 months after initial diagnosis. At necropsy, a focus of neoplastic cells was present in one lymph node, but except for skin other organs were not involved. The clinical presentation of histiocytic sarcoma may be unusual, and neoplastic cells may lack overt features of malignancy on cytologic and histopathologic examination. In some instances, immunophenotyping is required to differentiate histiocytic sarcoma from other histiocytic disorders.     

斯氏艾美耳球虫ITS1-5.8S rRNA-ITS2序列的克隆及PCR检测方法的建立

通过对多种鸡球虫和松鼠球虫18SrRNA和28SrRNA进行序列比对分析,在18SrRNA 3′端和28SrRNA 5′端保守区设计艾美耳属通用引物,以斯氏艾美耳球虫洛阳分离株LY卵囊基因组DNA为模板首次成功克隆到斯氏艾美耳球虫完整的ITS1-5.8SrRNA-ITS2序列,其大小为1 178bp,其中ITS1序列长度为423bp,5.8SrRNA为155bp,ITS2为600bp,斯氏艾美耳球虫LY株ITS1/2序列高度变异,与鸡球虫、啮齿动物球虫的序列相似性低于60%。然后在斯氏艾美耳球虫ITS1/2序列超变区设计种特异引物,建立了灵敏、特异的PCR检测方法。本研究结果将为兔球虫强致病种的临床诊断和揭示兔球虫种群遗传特征提供有效的分子工具。     

Effect of Estradiol-17β on protein synthesis and degradation rates in fused bovine satellite cell cultures

Although androgenic and estrogenic steroids are widely used to enhance muscle growth and increase feed efficiency in feedlot cattle, their mechanism of action is not well understood. Further, in vivo studies indicate that estradiol (E2) affects muscle protein synthesis and/or degradation, but in vitro results are inconsistent. We have examined the effects of E2 treatment on protein synthesis and degradation rates in fused bovine satellite cell (BSC) cultures. Additionally, to learn more about the mechanisms involved in E2-enhanced muscle growth, we have examined the effects of compounds that interfere with binding of E2 or insulin-like growth factor (IGF)-1 to their respective receptors on E2-induced alterations in protein synthesis and degradation rates in BSC cultures. Treatment of fused BSC cultures with E2 results in a concentration-dependent increase (P < 0.05) in protein synthesis rate and a decrease (P < 0.05) in protein degradation rate. The pure estrogen antagonist ICI 182 780 suppresses (P < 0.05) E2-induced alterations in protein synthesis and degradation in fused BSC cultures. The G-protein coupled receptor (GPR)-30 agonist G1 does not affect either synthesis or degradation rate, which establishes that GPR30 does not play a role in E2-induced alterations in protein synthesis or degradation. JB1, a competitive inhibitor of IGF-1 binding to the Type 1 insulin-like growth factor receptor (IGFR-1), suppresses (P < 0.05) E2-induced alterations in protein synthesis and degradation. In summary, our data show that E2 treatment directly alters both protein synthesis and degradation rates in fused BSC cultures via mechanisms involving both the classical estrogen receptor (ER) and IGFR-1.     

西北地区8种耐旱植物营养成分在秦川肉牛瘤胃的降解情况

本试验采集了西北地区8种耐旱植物(柠条、油蒿、桑叶、苦马豆、千屈菜、香豆子、元宝枫叶、无芒雀麦),选用4头体重相近的秦川牛肉用新品系瘤胃瘘管公牛进行试验,探究8种植物干物质(DM)、粗蛋白(CP)、中性洗涤纤维(NDF)、酸性洗涤纤维(ADF)的瘤胃降解特性。结果：常规营养成分：CP含量从小到大依次为无芒雀麦、千屈菜、油蒿、元宝枫叶、柠条、香豆子、桑叶、苦马豆(P<0.05);元宝枫叶与油蒿NDF、 ADF含量相近(P>0.05),其他植物均存在显著差异(P<0.05)。瘤胃降解特性：桑叶与苦马豆的DM降解效果显著高于其他6种植物(P<0.05),无芒雀麦48 h的 DM降解率仅29.56%,显著低于其他植物(P<0.05),油蒿、千屈菜、元宝枫叶48 h时DM降解情况差异不显著(P>0.05);CP的ED值从小到大依次为无芒雀麦、元宝枫叶、千屈菜、油蒿、柠条、香豆子、桑叶、苦马豆(P<0.05);NDF的ED值从小到大依次为无芒雀麦、元宝枫叶、油蒿、千屈菜、柠条、香豆子、苦马豆、桑叶(P<0.05),ADF的ED值从小到大依次为无芒雀麦、元宝枫叶、油蒿、千屈菜、苦马豆、香豆子、柠条、桑叶,其中柠条、苦马豆、香豆子和油蒿、元宝枫叶差异不显著(P>0.05)。     

MDCC—MSB1培养基上清液中DNA活性的定性研究

将 Ｍ Ｄ Ｃ Ｃ Ｍ Ｓ Ｂ１ ４８ 小时培养物 １０００ｒ／ｍ ｉｎ 离心的上清液分别用 Ｒ Ｎ Ａ 酶、 Ｄ Ｎ Ａ 酶和蛋白酶 Ｋ 处理后,进行体外试验。结果表明,只有 Ｄ Ｎ Ａ 酶处理后的上清液失去了体外抑制 Ｍ Ｄ Ｖ“８１４”增殖的作用。将该上清液 １００００ｒ／ｍ ｉｎ 离心所得的沉淀分别用上述酶处理后进行体内试验。结果表明,只有 Ｄ Ｎ Ａ 酶处理的样品失去了体内促进 Ｍ Ｄ Ｖ 京１ 株致瘤的作用。同时,电泳分析结果证明,该上清液中确实存在 Ｄ Ｎ Ａ。     

Leukocyte differentiation antigens in canine cutaneous and oral plasmacytomas

Seventeen cutaneous and oral tumours with light microscopic features of plasmacytomas from 16 dogs were studied. Clinically, most neoplasms were benign, although three recurred after excision and three were locally invasive. Tumours most often arose on the pinnae, digits, gingiva, and inguinal regions near areas of chronic inflammation and exhibited variable degrees of plasmacytic differentiation microscopically. Diagnosis of plasmacytoma was confirmed in paraffin-embedded tissues with a panel of leukocyte differentiation antigen markers that included cross-reactive antibodies for Mb-1 (CD79a), CD3, and vimentin and canine-specific antibodies for CD45RA and CD18. Immunoreactivity for Mb-1 and CD45RA, including staining of multinucleate cells and cells with karyomegaly, confirmed a B-cell origin of neoplasms, while staining for CD3 and CD18 revealed an extensive network of infiltrative T-cells and dendritic cells in tumours suggestive of a directed immune response. These findings (i) demonstrate the value of using a panel of antibodies for leukocyte antigens to differentiate plasmacytomas from other cutaneous and oral round cell tumours, and (ii) suggest that immune recognition and responsiveness within tumours may play a role in the behaviour of plasmacytomas in dogs by affecting tumour cell growth and differentiation.     

乳牛口服过量维生素A对维生素D3及钙磷代谢的影响

从北京郊区某农场选取１０头健康成年黑白花乳牛，随机分为试验（５头，每周每头口服维生素Ａ１００万单位）和对照（５头，不作任何处理）两组。８周以后检测发现，试验组血清钙和无机磷水平呈下降趋势；尾椎骨皮质变薄，密度下降，呈现骨质吸收现象；血清碱性磷酸酶显著下降（Ｐ＜０．０５）；其活性代谢产物２５－羟维生素Ｄ３（２５－ＯＨ－Ｄ３）极显著下降（Ｐ＜０．０１）；１，２５－二羟维生素Ｄ３［１，２５－（ＯＨ）２－     

影响棕色脂肪组织产热活性因素的研究进展

全球性肥胖流行问题愈发严峻,而脂肪组织本身可能是解决这个问题的关键。人和哺乳动物体内存在两种脂肪组织,发挥着截然相反的作用。与白色脂肪组织存储机体过剩的能量不同,棕色脂肪组织中存在独特的解偶联蛋白,能将脂肪酸氧化磷酸化,释放热能,增加能量消耗。因此,通过激活棕色脂肪组织产热,加速体内储存的脂质氧化磷酸化,成为了一种新的预防和治疗肥胖的手段。论文阐述影响棕色脂肪产热活性的因素以及相关机制,旨在为肥胖的治疗提供新的思路和方向。     

成都麻羊乳上皮黏蛋白MUC1的遗传多态性研究

采用SDS-PAGE研究成都麻羊乳上皮黏蛋白MUC1的遗传多态性,并以金堂黑山羊、乐至黑山羊、自贡黑山羊、藏山羊作对照进行比较分析。结果表明:成都麻羊与对照山羊乳MUC1的多态性丰富,成都麻羊有7种基因型、5个等位基因优势基因为C、D、E,金堂黑山羊7种基因型、6个等位基因优势基因为B、C、D,乐至黑山羊6种基因型、6个等位基因优势基因为B、C、D,自贡黑山羊5种基因型、5个等位基因优势基因为A、C、D,藏山羊3种基因型、3个等位基因优势基因为C、D。乳MUC1的基因型、等位基因的分布品种间有差异;根据乳MUC1等位基因频率对供试山羊品种进行聚类分析,成都麻羊与金堂黑山羊的遗传距离最小(D=0.2507)先聚为一类,再依次与乐至黑山羊(D=0.2831)、自贡黑山羊(D=0.3721)、藏山羊(D=0.4752)聚为一大类,结果较好的反映了成都麻羊与对照山羊品种间的亲缘关系。     

２，４-二叔丁基苯酚对啤酒花幼苗生长与光合特性的影响

前期对不同植龄啤酒花根系分泌物及根际土壤的化学物质进行了分离鉴定,结果发现有酚类物质的存在且２,４-二叔丁基苯酚含量较高,为了进一步证实啤酒花是否存在自毒作用,采用田间小区栽培试验,研究了不同用量［０（ＣＫ,Ａ０）,７．５（Ａ１）,１５．０（Ａ２）和２２．５ｍｍｏｌ／ｍ２（Ａ３）］２,４-二叔丁基苯酚处理对啤酒花幼苗生长及光合特性的影响。结果表明,２,４-二叔丁基苯酚用量Ａ１ 和Ａ２ 处理,啤酒花生长好,地上部、地下部、总生物量及日均光合速率均比对照高,且达到显著差异（犘＜０．０５）;２,４-二叔丁基苯酚用量Ａ３ 处理,啤酒花地上部、地下部和总生物量及光合特性均较对照降低,差异达到显著水平（犘＜０．０５）。啤酒花幼苗叶片光合速率日变化呈双峰型,最高峰出现在上午９：００左右,次高峰出现在中午１５：００左右,午间有明显的“午休“现象,与对照（Ａ０）相比,Ａ１、Ａ２、Ａ３ 三个处理的次高峰出现的时间均有推迟,且Ａ１ 和Ａ２ 处理的幼苗午休”现象不太明显,气孔因素是导致其“午休”的主要原因。