棉铃虫围食膜中Bt抗性相关蛋白的分离与鉴定

棉铃虫是世界性的重要农业害虫。围食膜作为昆虫抵御病原微生物入侵及有害物质的第一道天然保护性屏障,其上可能存在与Bt抗性相关的受体蛋白。本研究以Bt Cry1Ac抗性和敏感品系的棉铃虫围食膜为对象,采用NuPAGE电泳技术、配体杂交、质谱鉴定和生物信息学分析等技术,测定了围食膜蛋白含量,鉴定了蛋白质的组成及与Bt Cry1Ac毒素的结合能力。结果表明敏感品系围食膜中蛋白含量为22.19%,抗性品系围食膜中蛋白含量为26.99%。抗、感品系棉铃虫围食膜上存在与Cry1Ac毒素结合的6个差异蛋白,推测其中棉铃虫羧酸酯酶蛋白和血影蛋白是2个有意义的抗性相关蛋白,2个新蛋白可能参与Bt抗性。研究证明棉铃虫围食膜上存在Bt结合蛋白且与抗性相关,为进一步明确Bt抗性机制、制定合理的Bt抗性治理策略提供了理论依据。     

豆制品加工中黄浆水无害化处理技术研究

对豆制品中黄浆水进行超滤处理的工艺参数进行研究。用外加压管式膜组件对黄浆水进行了超滤试验,分析了液体分别在不同的温度、压力、流速、pH值等因素下对渗透流量和截留率的影响。通过正交试验,得到了处理黄浆水废液的最佳工况。实验结果表明,超滤处理黄浆水的最佳工况是:在压力0.15MPa下,用泵的最大流量对刚排放的黄浆水(温度55℃左右)进行超滤处理,可回收全部脂肪、75%以上的蛋白质和70%以上的总糖。回收后的浓缩物(或干燥物)可以作为饲料或经纯化后食用。     

Bubbleless Aerated Membrane Bioreactor for Ramie Degumming Wastewater Treatment

Through introducing the principle of bubbleless aerated membrane bioreactor, the traditional membrane bioreactor was improved. It could be obtained that the removal efficiency of COD and NH3 - N in bubbleless aerated membrane bioreactor increased 4% and 2% than that in traditional membrane bioreactor, respectively. Adopting bubbleless aeration as reflushing air which not only increased DO in the MBR, but also advanced the oxygen utilization, and the energy consumption decreased as well.     

Deformation of deep foundation pits due to excavation and dewatering considering the change of void ratio and permeability coefficient with current stress of soil

基于修正剑桥模型理论,推导了孔隙比e随土体当前应力变化的方程,同时比选了4组经典的描述渗透系数k随孔隙比变化的方程,选择了其中一组最佳的估算公式,编写ABAQUS用户子程序VOIDRI和USDFLD,以实现孔隙比和渗透系数随土体当前应力的变化。在此基础上,研究深基坑降水开挖所致的坑内外土体的变形、围护结构的变形及弯矩,得到以下结论：当考虑孔隙比随土体当前应力变化时,坑外地表沉降量、墙体的水平位移、地下连续墙的弯矩、坑底隆起量均大于孔隙比为定值时的情况;当考虑渗透系数随土体当前应力变化时,坑外地表沉降量、墙体的水平位移、地下连续墙的弯矩均小于渗透系数为定值时的情况,但同时考虑渗透系数和孔隙比变化情况时,其对坑底的隆起量的影响可以忽略不计。     

Mathematical Model of Methane Transport in Coal Seam

This paper studies the effects of ground stress and ground electrical field onmethane transpont in coal seam. A gas seepage equation is set up based on the principles of seepagetheory,which takes the ground stress and ground electrical field into considerationz     

水分胁迫对冬小麦旗叶细胞质膜及叶肉细胞超微结构的影响

随着水分胁迫程度的加剧，冬小麦叶的膜质过氧化物丙二醛的含量升高，膜脂 酸不饱和度降低，叶肉细胞内叶绿体、线粒体的形态、结构异常，甚至裂解，质膜内陷，胞液泡化，胞壁层次减少。表明土壤水分胁迫损害小麦旗叶细胞质膜及叶肉细胞的超微结构，加速衰老进程。     

隔膜计量泵的改良设计与汽蚀性能的提高

针对应用于石油化工装置上的进口隔膜计量泵容易产生的汽蚀问题，对隔膜泵复合隔膜、柱塞密封副、液压平衡与补偿阀结构进行了改良设计，采用较大承载能力的机座、独特阶梯式活塞、加大直径的活塞、PTFE＋NBR复合隔膜，改良放气安全阀及油浸没式补偿系统等方法，提高了隔膜计量泵的汽蚀性能；通过对改造的隔膜计量泵与原有的计量泵进行的汽蚀试验对比，给出了隔膜计量泵性能与现场使用的最佳匹配工况。改良的结构设计和新型材料的选用大大提高了计量泵的汽蚀性能。     

不同降解阶段填埋垃圾体的气体渗透特性研究

通过对不同降解阶段填埋垃圾体渗透系数的测定，研究了在不同压实密度、不同含水率条件下垃圾体渗透特性的变化情况，实验表明：1.对不同降解阶段的填埋垃圾体，新鲜垃圾及混和垃圾的渗透系数接近，腐熟垃圾明显高于前两者；随压实密度增加，其气体渗透系数均呈指数规律递减；2.随着垃圾体含水率的增加，腐熟与新鲜垃圾的渗透系数均呈线性规律递减，含水率对渗透系数的影响要远小于压实密度的影响；3.新垃圾、混合垃圾、腐熟垃圾均具有较显著的各向异性特征，在典型压实密度下，水平方向的气体渗透系数Kh为垂直方向渗透系数Kv的3.5-27.3倍。     

基于WS和实际耗水量的膜下滴灌

通过田间试验，建立了膜下滴灌棉花CWSI和水汽饱和差VPD的定量关系，确定了棉花各生育阶段CWSI下基线的特定表达形式。对膜下滴灌条件下棉花生长发育、光合动态与根系分布规律以及不同水分处理的耗水量、产量与品质指标进行了观测。对不同水分处理棉花的CWSI进行了定期观测，得到了棉花CWSI与棉花耗水量的关系。     

Concentration-Dependent Mobility of Chlorfenvinphos in Isolated Plant Cuticles

The mobility of chlorfenvinphos in isolated pear (Pyrus communis cv. Bartlett) leaf cuticular membranes (CM) was studied as a function of concentration of chlorfenvinphos sorbed in the cuticle. Mobilities of chlorfenvinphos increased approximately 9-fold when the amount sorbed increased from 1 to 100 μg cm⁻² pear leaf cuticle. From the amounts per area, average volume fractions of chlorfenvinphos in the cuticle were calculated ranging from 2×10⁻³ to 5·1×10⁻². The increase in mobilities was steepest at the lower and levelled off at higher volume fractions. This correlation could be described for the whole range of volume fractions investigated by an equation which assumes homogeneously dispersed chlorfenvinphos. Temperature dependence of mobilities was studied at 17, 25 and 35°C and chlorfenvinphos volume fractions of 5·5×10⁻³ and 0·12, respectively. Arrhenius graphs were linear for both volume fractions, showing that cuticles did not undergo a phase transition due to the high amount of sorbed chlorfenvinphos. However, at a volume fraction of 0·12, the activation energy of diffusion, E_D, was significantly lower (83·6 kJ mol⁻¹) than at 5·5×10⁻³ (135 kJ mol⁻¹). We interpret these findings as evidence for a plasticising effect on cuticular waxes by chlorfenvinphos. So far, such an effect had been demonstrated only for certain adjuvants (ethoxylated alcohols) but not for active ingredients. Chlorfenvinphos not only increased its own mobility in pear leaf cuticles, but also that of 2,4-D in Citrus leaf cuticles. This would be expected if plasticising of waxes was the sole mechanism responsible for increased mobilities. From these data we predict that permeabilities of cuticles to chlorfenvinphos are not constant. Depending on temperature as well as types and amounts of adjuvants, rates of foliar penetration of chlorfenvinphos can be higher if its concentration in the spray liquid is increased.