贯叶连翘无菌苗茎尖诱导变异体研究

以研究贯叶连翘离体诱变及筛选变异体为目的,用贯叶连翘无菌苗茎尖为供试材料,研究了平阳霉素处理浓度、时间、外源添加物对供试茎尖存活率以及激素配比对茎尖不定芽诱导率的影响。结果表明：平阳霉素80mg/L的20min处理可作为适宜诱变处理,添加椰汁有利于茎尖存活;6-BA0.5mg/L、NAA0.01mg/L的激素组合有利于诱导正常型不定芽和获得较高的诱导率。通过研究,建立了贯叶连翘变异体诱导技术程序,获得了金丝桃素含量提高的变异体材料。     

贯叶连翘茎段和叶片的离体培养及植株再生研究

以贯叶连翘的叶片和茎段作为外植体,培养在含有不同种类及浓度激素的MS基本培养基上,进行愈伤组织、丛生芽、根的诱导实验,并进行试管苗的移栽。结果表明,2,4-D有利于贯叶连翘愈伤组织的形成;加有6-BA 0.5 mg/L的MS培养基最适合丛生芽的形成;诱导生根较好的培养基是MS+IBA 0.5 mg/L +NAA 0.5 mg/L和MS+IBA 1.0 mg/L。     

贯叶连翘无菌苗茎尖诱导变异体研究

以研究贯叶连翘离体诱变及筛选变异体为目的,用贯叶连翘无菌苗茎尖为供试材料,研究了平阳霉素处理浓度、时间、外源添加物对供试茎尖存活率以及激素配比对茎尖不定芽诱导率的影响。结果表明:平阳霉素80mg/L的20min处理可作为适宜诱变处理,添加椰汁有利于茎尖存活;6-BA0.5mg/L、NAA0.01mg/L的激素组合有利于诱导正常型不定芽和获得较高的诱导率。通过研究,建立了贯叶连翘变异体诱导技术程序,获得了金丝桃素含量提高的变异体材料。     

贯叶金丝桃离体培养及植株再生体系的建立

[目的]研究贯叶金丝桃的组培快繁技术,以提高其繁殖率和生根率,并建立一套高效的离体培养再生体系。[方法]以贯叶金丝桃幼芽为外植体,对适宜的丛生芽繁殖和生根培养基进行了筛选。[结果]适宜的繁殖培养基为MS+6-BA 1.0 mg/L+IAA 0.1 mg/L;适宜的生根培养基为1/2 MS+IAA 0.5 mg/L+ABT 0.2 mg/L。[结论]研究结果为贯叶金丝桃的种质保存和良种选育工作奠定了基础。     

贯叶金丝桃过氧化氢酶基因的生物信息学分析

利用生物信息学软件分析了贯叶金丝桃过氧化氢酶基因编码蛋白的氨基酸组成、理论等电点、结构域、特征位点、二级结构等蛋白质性质。结果表明：贯叶金丝桃过氧化氢酶基因编码蛋白分子式为：C2580H3880N718O728S13,属于稳定蛋白;二级结构以无规则卷曲为主。     

Changes of weed infestation under long-term effect of different soil pH levels and amount of phosphorus:potassium

ABSTRACT

The aim of the present study was to investigate changes of weed species density as a result of long-term (1976–2005) exposure to different soil pH levels, and P₂O₅:K₂O amount in soil created by initial and subsequent periodical liming, and fertilization. As a result of liming during the period 1976–2005, average soil pH levels at the start of the sixth crop rotation (2001–2005) ranged from 4.1 (unlimed) to 6.6. And in each pH plot were four sub-plots with a different amount of mobile phosphorus:potassium. In acid soil (pH 4.1), in spring oilseed rape and in spring barley crops, the dominant weed Spergula arvensis density decreased significantly at a pH of 5.1 or higher. With increasing amount of P₂O₅:K₂O in soil, the abundance of S. arvensis in spring rape and spring barley crops was reduced at all soil pH levels. At pH 5.1, Chenopodium album and Tripleurospermum perforatum were prominent. Elytrigia repens tended to decrease with increased alkalinity and nutrient amount in soil. With reduction of soil acidity from pH 4.1 to 6.6, the total weed infestation consistently declined in all crops. A low amount of P₂O₅:K₂O in soil caused the decline in the abundance of S. arvensis, E. repens and increase in C. album and T. perforatum.     

贯叶金丝桃ISSR反应体系的建立与优化

以药用植物贯叶金丝桃为研究对象,对其ISSR—PCR反应体系中模板DNA用量、Mg2＋浓度、dNTP浓度、Taq酶含量及退火温度等因素进行了梯度试验,得到最佳ISSR—PCR反应体系,并初步筛选出13条条带清晰、多态性高、重复性好的ISSR引物,为贯叶金丝桃遗传多样性及种质资源鉴定等方面的研究奠定基础。     

南京地区贯叶金丝桃的引种试验

2000年至2007年,对分布于中国西南4省13个样点的贯叶金丝桃(Hypericum perforatum)引种至南京后的生长发育规律进行了比较研究.观察其播种出苗情况和茎、叶、花等器官的植物学性状表现.对其形态学及内含物成分的比较分析结果表明,异地栽培后,贯叶金丝桃仍保持原有的生物学特征.同时对贯叶金丝桃在南京地区的不同栽培方式、管理技术等进行了初步探讨.     

RFLP analysis of Hypericum perforatum L. somaclones and their progenies

Three adjacent EcoRI fragments of the rDNA unit from Lycopersicon esculentum, eleven anonymous genomic and two anonymous cDNA clones from Brassica napus and three restriction endonucleases: BamHI, EcoRI and EcoRV were used for RFLP analysis of the genome of Hypericum perforatum L. A polymorphic band identified with EcoRI and two rDNA probes in five somaclones originated from the same genotype was detected in all progenies of two somaclones indicating the inheritance of the molecular changes. rDNA unit heterogeneity represented by two types of RFLP pattern revealed among somaclones and seed-derived control plants using EcoRV and two rDNA probes may indicate an alloploid origin of Hypericum perforatum. The occurrence of the identical RFLP patterns in some R₀ somaclones and seed-derived plants and their progenies may be related to the apomictic mode of reproduction which is assumed to be prevalent in Hypericum perforatum. The differences in RFLP patterns of progenies when compared with the maternal plants (1 out of 10 progenies of one control plant and 1 out of 8 progenies of one somaclone) may indicate that some progenies have originated through sexual recombination. This revised version was published online in July 2006 with corrections to the Cover Date.     

贯叶金丝桃余渣栽培猴头菌和金针菇的研究

试验表明,贯叶金丝桃余渣适合栽培猴头菌（Ｈｅｒｉｃｉｕｍ ｅｒｉｎａｃｅｕｓ）和金针菇（Ｆｌａｍｍｕｌｉｎａ ｖｅｌｕｔｉｐｅｓ）。用贯叶金丝桃余渣栽培的猴头菌具有较高的超氧化物歧化酶（ＳＯＤ）活力。