Experimental studies with foot-and-mouth disease virus type Asia-1, responsible for the 2005 epidemic in China

This study was carried out to investigate the biological characteristics of the foot-and-mouth disease (FMD) virus strain Asia-1 China/2005, which is responsible for the 2005 epidemic in China. The result showed that this strain is not host restricted, and could not only cause FMD in cattle and sheep but also in pigs by either inoculation or direct contact.     

天然植物制剂“开口健”对小鼠淋巴细胞功能及营养生长因子的影响

目的：探讨口服＂开口健＂对小鼠淋巴细胞功能以及营养生长因子的影响。方法：36只ICR小鼠随机分为4组,即生理盐水组＂,开口健＂低、中、高剂量组。给小鼠连续灌胃10 d后,用MTT法检测淋巴细胞刺激指数,RT-PCR方法检测ConA刺激后淋巴细胞产生的IL-4、IL-10、IL-12和INF-γmRNA水平,ELISA方法检测小鼠血清胰岛素样生长因子（IGF-1）和生长激素释放肽（Ghrelin）水平。结果：口服＂开口健＂小鼠脾脏淋巴细胞SI显著高于对照组（P〈0.01）,并随＂开口健＂剂量的增加而升高;口服＂开口健＂小鼠脾脏淋巴细胞产生IL-4、IL-10、IL-12和IFN-γmRNA水平显著高于对照组（P〈0.05）。口服＂开口健＂显著提高了小鼠血清的IGF-1和Ghrelin水平（P〈0.05）,且与＂开口健＂剂量呈正相关性。结论：口服＂开口健＂可增强小鼠淋巴细胞功能并提高血清营养生长因子水平。     

线虫fat-1基因密码子优化设计

本研究旨在优化线虫fat-1基因,使其在牛肌肉组织中高效表达,为进一步生产fat-1转基因肉牛提供目的基因。试验在不改变fat-1基因编码氨基酸序列的基础上,参考了11个牛肌肉蛋白相关基因的密码子使用频率,对fat-1基因进行了密码子优化。结果发现,共改变了65个碱基,涉及65个密码子、7个氨基酸,约占fat-1基因总碱基数的5.38%,G+C含量从45.08%上升到50.04%,且分布均匀利于基因的表达。结构预测结果显示,该优化后的基因能够在牛肌肉中高效表达。     

Porcine CD18 mediates Actinobacillus pleuropneumoniae ApxIII species-specific toxicity

Actinobacillus pleuropneumoniae, the causative agent of porcine pleuropneumonia, produces Apx toxins that are recognized as major virulence factors. Recently, we showed that ApxIIIA-cytotoxic activity specifically targets Sus scrofa leukocytes. Since both LtxA from Aggregatibacter actinomycetemcomitans (aggressive periodontitis in humans) and LktA from Mannheimia haemolytica (pneumonia in ruminants) share this characteristic, respectively towards human and ruminant leukocytes, and because both use the CD18 subunit to interact with their respective LFA-1, we hypothesized that ApxIIIA was likely to bind porcine CD18 to exercise its deleterious effects on pig leukocytes. A β ₂−integrin-deficient ApxIIIA-resistant human erythroleukemic cell line was transfected either with homologous or heterologous CD11a/CD18 heterodimers using a set of plasmids coding for human (ApxIIIA-resistant), bovine (-resistant) and porcine (-susceptible) CD11a and CD18 subunits. Cell preparations that switched from ApxIIIA-resistance to -susceptibility were then sought to identify the LFA-1 subunit involved. The results showed that the ApxIIIA-resistant recipient cell line was rendered susceptible only if the CD18 partner within the LFA-1 heterodimer was that of the pig. It is concluded that porcine CD18 is necessary to mediate A. pleuropneumoniae ApxIIIA toxin-induced leukolysis.     

不同天然草地开垦年限下土壤特性对CH4吸收的影响

通过培养实验,称取过2mm筛的风干土样50g于300mL的培养瓶中,调节含水量至25%(W/W)后密封,在(25±1)℃条件下恒温培养一周,探讨内蒙古锡盟太仆寺旗典型草原天然草地转变为农田后,在相同的水分温度条件下,不同开垦年限(5、10、50年)农田的土壤特性对CH₄吸收的影响。结果表明:天然草地转变为农田后降低了土壤吸收CH₄的能力,开垦年限对CH₄吸收有显著影响(F=20.998,P<0.001),CH₄的吸收量随开垦年限的增加而逐渐降低,4个样地土壤CH₄的吸收量按大小顺序为:天然草地>开垦5年的农田>开垦10年的农田>开垦50年的农田,以天然草地为对照,开垦5年、10年、50年后农田土壤CH₄的吸收量分别降低了17.1%、44.1%、50.1%;CH₄的累积吸收量随土壤有机碳、全氮含量和微生物生物量碳、氮的降低而降低,单因子相关分析表明,土壤有机碳含量、全氮含量、土壤微生物量碳、氮是影响土壤CH₄吸收的主要因子。     

Characteristics of 18F-FDG and 18F-FDOPA PET in an 8-year-old neutered male Yorkshire Terrier dog with glioma: long-term chemotherapy using hydroxyurea plus imatinib with prednisolone and immunoreactivity for PDGFR-β and LAT1

An 8-year-old neutered male Yorkshire Terrier dog presented with head pressing, vestibular ataxia, neck tenderness, and no oculocephalic reflex. A demarcated lesion in the pons was identified on MRI. The patient was tentatively diagnosed with a glioma and was treated with hydroxyurea plus imatinib and prednisolone. After 30 days of therapeutic treatment, the patient showed a clear improvement in neurological signs, which lasted for 1117 days. On day 569 after the initiation of treatment, ¹⁸F-fluorodeoxyglucose (FDG)-positron emission tomography (PET) was performed with no significant findings on visual analysis. The average and maximal standardized uptake values (SUVs) were 1.92 and 2.29, respectively. The tumor-to-normal-tissue (T/N) ratio was 0.97. The first evidence of clinical deterioration was noticed on day 1147. On day 1155, 3,4-dihydroxy-6-[¹⁸F]-fluoro-l-phenylalanine (¹⁸F-FDOPA)-PET was performed. High uptake of ¹⁸F-FDOPA was observed in the intracranial lesion. The mean and maximal SUVs of the tumor were 1.59 and 2.29, respectively. The T/N ratio was 2.22. The patient was euthanized on day 1155 and histopathologic evaluations confirmed glioma (astrocytoma). This case shows that chemotherapy with hydroxyurea plus imatinib may be considered in the treatment of canine glioma. Furthermore, this is the first case describing the application of ¹⁸F-FDG and ¹⁸F-FDOPA in a dog with glioma.     

人工感染猪流行性腹泻病毒仔猪外周血SmIg+B细胞及T细胞的动态观察

本实验对猪流行性腹泻病毒(PEDV)—J毒株细胞培养毒接种的6头仔猪和3头健康仔猪外周血SmIg~+B细胞及T淋巴细胞的数量作了检测。T细胞用总E玫瑰花(Et)及活化E玫瑰花(Ea)试验作检测,SmIg~+B细胞用荧光素标记的葡萄球菌A蛋白(FITC—SPA)菌体法鉴定。结果:接种后,SmIg~+B细胞百分率显著上升,于第23天时达峰值(29.19±3.45),明显高于对照猪(P<0.05),Et玫瑰花百分率虽略有上升,但与接种前相比无统计学差异(P>0.05),而Ea玫瑰花百分率则显著低于接种前水平(P<0.01)。以上结果表明,PEDV—J毒株细胞培养毒可刺激仔猪B细胞明显增多。由此认为,B淋巴细胞积极参与PED的免疫反应过程,体液免疫是PED猪的主要免疫反应。     

microRNA-146a通过靶向MAPK4和Myosin Va基因调控羊驼黑色素细胞增殖、迁移

旨在研究microRNA-146a(miR-146a)对羊驼黑色素细胞增殖和迁移的调控及其分子机制.本研究使用双荧光素酶试验验证MAPK4和Myosin Va是miR-146a的靶基因;利用荧光定量PCR和蛋白质免疫印迹试验检测在羊驼黑色素细胞中过表达miR-146a对相关下游基因表达的影响;利用CCK8和Transw...     

蓝狐与银狐褪黑激素受体1A基因的克隆及序列分析

根据其它物种褪黑激素受体（melatonin receptor, MTNR）1A基因核酸序列的同源性设计引物,PCR扩增蓝狐和银狐MTNR1A基因的546 bp DNA序列,GenBank登录号分别为EU170442和EU170443。蓝狐和银狐MTNR1A基因DNA序列同源性为99.1%,编码的氨基酸同源性为99.4%,与人、鼠及绵羊MTNR1A基因同源性为84%~86%,与鸡MTNR1A基因同源性为74%。