温室三七收获机有限元分析

根据三七种植新农艺的要求,设计了一款应用于温室种植模式的三七收获机。为提高其工作性能,利用ANSYS有限元软件进行有限元分析,获取在静载荷作用应力、变形的大小及分布情况。结果表明:应力集中发生在电机安装位置及车架纵梁连接处,最大变形发生在车尾处,机架强度符合要求。对机架进行模态分析,获得其固有频率并进行谐响应分析,分析可知:在频率15Hz时电机安装位置处可能发生共振,此时电机安装位置处应力和位移达到最大,最大应力为4.34MPa,最大位移为2.03mm,机架满足激励载荷下的强度要求。对挖掘铲进行静力学分析,得到挖掘阻力5 000N,挖掘铲最大变形量为1.334 4mm,最大应力为20.894MPa。本文为后续温室三七收获机的减振以及机架、挖掘铲优化提供了理论依据。     

Analysis of the IgG antibody response against Paramphistomidae trematoda in naturally infected cattle. Application to serological surveys

The IgG antibody response to Calicophoron daubneyi (Digenea: Paramphistomidae) excretory/secretory antigens was evaluated in naturally infected cattle from Lugo (Galicia, NW Spain) by using an ELISA procedure. Two studies were conducted, first a survey in 524 cattle separated into three groups according to age, G-1 (0–2 years old), G-2 (3–5 years old) and G-3 (>6 years old). In the second study, three groups of cattle were employed: G-I, naturally infected; G-T, naturally infected and treated with oxyclozanide plus levamisole (Nilzan Plus^®); G-C, cattle maintained in a farm where C. daubneyi has never diagnosed. Variations on egg-output and haematic parameters (erythrocytes, haematocrite, leukocytes and lymphocytes) were also analyzed.

The ELISA procedure showed that 61.2% of the cattle in the first study had been exposed to the trematode, but only 10.1% passed eggs in the feces. Age-association with egg-output was shown but not with the IgG values. In the second experiment, the administration of the anthelmintic reduced significantly the IgG kinetic levels and the C. daubneyi-egg-output was suppressed during 12 weeks in the G-T group. The values of red cells, haematocrite, leukocytes and lymphocytes increased significantly in the treated cattle 5 weeks after chemotherapy; however, new reduction after week 5 was recorded, as results of the challenge of these cattle.

This is the first investigation in which evaluation of the IgG humoral response against C. daubneyi in cattle has been carried out. We proved that a notable IgG response in naturally infected cattle is induced, and can be detected by using an ELISA procedure. The IgG antibodies did not increase after challenge infection. Our results proved an important percentage of cattle were exposed to this trematode in the area of study and suitable measures for preventing this relationship must be considered.     

敌草快和硫辛酸对育肥猪肠道结构及消化功能的影响

本文旨在研究敌草快和硫辛酸(LA)对育肥猪肠道结构及消化功能的影响,以探究LA对敌草快引起的应激是否有缓解作用。选取24头(70.64±3.61)kg的健康大白阉公猪,按照2×2因子设计,随机分为对照组、LA组、敌草快组和LA+敌草快组,每个组设6个重复,每个重复1头猪。试验期29 d。LA添加剂量为800 mg/kg饲粮,饲喂贯穿试验全程,而一次性腹腔滴注敌草快于试验第15天进行,无腹腔滴注敌草快试验猪滴注等量的生理盐水。于试验第29天对所有试验猪前腔静脉采血后屠宰取样,运用试剂盒检测血浆和肠道氧化损伤标志物的含量以及空肠食糜消化酶的活性,苏木精-伊红(HE)染色后运用图像分析软件观察肠道形态结构并测定绒毛高度、隐窝深度,并计算绒毛高度/隐窝深度(V/C),荧光定量PCR法检测闭合蛋白(occludin)和紧密结合蛋白(ZO-1)的相对表达量。结果表明:1)敌草快极显著升高了试验猪血浆和肠道丙二醛(MDA)、蛋白质羰基(PCO)和8-羟基鸟苷(8-OHd G)的含量(P0.01),极显著降低了十二指肠、空肠及回肠绒毛高度、隐窝深度(P0.01),极显著增大了十二指肠和空肠V/C(P0.01),显著增大了回肠V/C(P0.05),极显著降低了空肠occludin和ZO-1 m RNA相对表达量(P0.01),极显著降低了空肠食糜中淀粉酶、胰蛋白酶以及脂肪酶的活性(P0.01)。2)饲粮中添加LA对试验猪血浆和肠道MDA、PCO和8-OHd G含量的影响均不显著(P0.05),对十二指肠、空肠和回肠的绒毛高度、隐窝深度以及V/C的影响均不显著(P0.05),对空肠occludin和ZO-1 m RNA相对表达量的影响也不显著(P0.05),对空肠食糜中淀粉酶、胰蛋白酶以及脂肪酶活性的影响差异也均不显著(P0.05)。3)在应激状态下,饲粮中添加LA使猪血浆8-OHd G含量显著降低(P0.05),十二指肠绒毛高度、空肠绒毛高度以及回肠隐窝深度显著升高(P0.05),空肠V/C显著降低(P0.05),回肠V/C极显著降低(P0.01),空肠occludin和ZO-1 m RNA相对表达量显著升高(P0.05),空肠食糜淀粉酶和胰蛋白酶的活性显著提升(P0.05)。由此可见,敌草快引起了育肥猪强烈的氧化应激,导致了肠道结构的严重损伤并减弱了肠道的消化功能;在敌草快引起的应激状态下,饲粮中添加800 mg/kg LA能够减缓肠道结构的损伤并能一定程度地提高肠道的消化功能。     

花岗岩黄红壤杉木幼林施肥连年生长效应

１９９１￣１９９５年试验结果表明，在江西分宜花岗岩残积物发育的黄红壤上，杉木幼林施Ｎ、Ｐ、Ｋ及配合处理，使树高、胸径、蓄积总生长量指标分别达到５．６７￣６．５９ｍ、８．３５￣９．２８ｃｍ、６７．９２２￣９６．４１ｍ＾３／ｈｍ＾２，较对照增长５．６％￣２０．２％、７．４％￣１７．５％、２１．６％￣５６．２％。在各种施肥处理中，以５０ｋｇ／ｈｍ＾２Ｐ２Ｏ５的效果最佳，其连年生长量效应显著持续到第５年；     

Pretreatment of Rice Straw by Hydrogen Peroxide for Enhanced Methane Yield

A pretreatment process for hydrogen peroxide (H2O2) was optimized to enhance the biodegradation performance of rice straw and increase biogas yield. A determination experiment was conducted under predicted optimal conditions. Optimization was implemented using response surface methodology. The effects of biodegradation and the interactive effects of pretreatment time (PT), H2O2 concentration (HC), and substrate to inoculum ratio (S/I) on methane yield were investigated. The lignin, cellulose, and hemicellulose of rice straw were significantly degraded with increasing HC. The optimal conditions for the use of pretreated rice straw in anaerobic digestion were a 6.18-d PT, 2.68% HC (w/w total solid), and 1.08 S/I; these conditions result in a methane yield of 288 mL g-1 volatile solids (VS). A determination coefficient of 95.2% was obtained, indicating that the model used to predict the anabolic digestion process has a favorable fit with the experimental parameters. The determination experiment resulted in a methane yield of 290 mL g-1 VS, 88.0% higher than that of untreated rice straw. Thus, H2O2 pretreatment of rice straw can be used to improve methane yields during biogas production.     

遮荫条件下9个紫花苜蓿品种苗期形态及生长响应

采用遮荫试验,研究了光对9个苜蓿品种苗期形态特征及生长动态的影响。结果表明,不同遮荫处理下苜蓿品种间株高变化有较大的差异,游客表现出最弱的耐阴性;随光照减弱,各品种根颈分枝数、主根粗、二级侧根数、根瘤数、叶片厚度和叶片数均显著下降,表现出较强光环境下更强的形态可塑性;各品种主根长无显著变化,总叶面积动态中新疆大叶、苜蓿王和德宝变化不显著,其他品种显著下降。各品种生物量随遮荫强度增加而显著下降,弱光下各品种生物量对根系的分配明显下降,对茎和叶的分配增加,叶面积比随光强减弱明显增大,相对生长速率随光强减弱显著下降。不同品种同一器官对光环境变化的反应程度不一致,反映了苜蓿各品种耐阴机制的差异。     

Chitosan nanoparticles enhance developmental competence of in vitro-matured porcine oocytes

Oxidative stress is inevitable as it is derived from the handling, culturing, inherent metabolic activities and medium supplementation of embryos. This study was performed to investigate the protective effect of chitosan nanoparticles (CNPs) on oxidative damage in porcine oocytes. For this purpose, cumulus–oocyte complexes (COCs) derived from porcine slaughterhouse ovaries were exposed to different concentrations of CNPs (0, 10, 25 and 50 µg/ml) during in vitro maturation (IVM). Oocytes treated with 25 µg/ml CNPs showed significantly higher levels of GSH, along with a significant reduction in ROS levels compared to control, CNPs10 and CNPs50 groups. In parthenogenetic embryo production, the maturation rate was significantly higher in the CNPs25 group than that in the control and all other treated groups. In addition, when compared to the CNPs50 and control groups, CNPs25-treated oocytes showed significantly higher cleavage and blastocyst development rates. The highest concentration of CNPs reduced the total cell number and ratio of ICM: TE cells in parthenogenetic embryos, suggesting that there is a threshold where benefits are lost if exceeded. In cloned embryos, the CNPs25 group, as compared to all other treated groups, showed significantly higher maturation and cleavage rates. Furthermore, the blastocyst development rate in the CNPs25-treated group was significantly higher than that in the CNPs50-treated group, as was the total cell number. Moreover, we found that cloned embryos derived from the CNPs25-treated group showed significantly higher expression levels of Pou5f1, Dppa2, and Ndp52il genes, compared with those of the control and other treated groups. Our results demonstrated that 25 µg/ml CNPs treatment during IVM improves the developmental competence of porcine oocytes by reducing oxidative stress.     

粘弹性地基中桩动力反应分析

分析了在桩头作用和谐和荷载时有限长桩与无限长桩的动力反应，并进行了对比，分析表明，在一般情况下，当桩长径比ｌ／ｄ≥１０时将有限长桩作为无限长桩来分析，所得内力与位移最大值误差差在工程允许范围内。     

Exogenous Application of Abscisic Acid Improves Cold Tolerance in Chickpea (Cicer arietinum L.)

Chickpea suffers cold stress (<10 °C) damage especially during reproductive phase resulting in the abortion of flowers and pods, poor pod set, and reduction in seed yield and seed quality. One of the ways in modifying cold tolerance involves exogenous treatment of the plants with chemicals having established role in cold tolerance. In the present study, the chickpea plants growing under optimum temperature conditions (28/12 °C, as average maximum and minimum temperature) were subjected to cold conditions of the field (10–12/2–4 °C; day/night as average maximum and minimum temperature) at the bud stage. Prior to exposure, these plants were treated exogenously with 10 μm abscisic acid (ABA) and thereafter again after 1 week of exposure. The stress injury measured in terms of increase in electrolyte leakage, decrease in 2,3,5-triphenyl tetrazolium chloride reduction %, relative leaf water content and chlorophyll content was observed to be significantly mitigated in ABA-applied plants. A greater pollen viability, pollen germination, flower retention and pod set were noticed in ABA-treated plants compared with stressed plants. The seed yield showed considerable improvement in the plants treated with ABA relative to the stressed plants that was attributed to the increase in seed weight, greater number of single seeded pods and reduction in number of infertile pods. The oxidative damage measured as thiobarbituric acid-reactive substances was lesser in ABA-treated plants that was associated with greater activities of superoxide dismutase, catalase, ascorbate peroxidase, ascorbic acid, glutathione and proline in these plants. It was concluded that cold stress effects were partly overcome by ABA treatment because of the improvement in water status of the leaves as well as the reduction in oxidative damage.     

生态自然保护区农民脱贫问题探析——湘西州“退人还山”脱贫模式调研分析

“退人还山”是在科学发展观指导下，一个来源于湘西州扶贫开发实践、服务于民族贫困山区农民新农村建设的理论，是国家易地扶贫战略的新发展。“退人还山”遵循了山区自然法则和经济规律，它是贫困山区农民改变贫困命运的根本出路，是国家生态建设的治本之策。