Histological Study on the Polyovular Folliclular Development of Chinese Prematural Lubei White Goat

The present study was conducted to investigate the histological characteristics of Polyovular follicles in Chinese Lubei white female goats with age 2-5 months. The results suggested that the healthy antral follicles with twin-oocyte could be observed. There were many multi-oocyte phenomena in primordial and primary follicles with a proportion of nearly 75% in primordial follicles. Primordial follicle was in the outer of cortex in ovaries and most of them remained active. The nest of multi-nuclear primordial follicle was developing into primordial follicle with 1 to 3 nuclei.     

热激对甜瓜未授粉子房培养胚珠膨大期内源激素的影响

为探究热激处理对甜瓜未授粉子房培养中胚珠膨大发育与内源激素的影响,以期为揭示胚珠膨大和单倍体培养的机理研究奠定基础。以热激和对照培养不同天数的甜瓜未受粉子房为试材,对胚珠膨大率进行动态监测,并采用UPLC-ESI-MS/MS法测定顺式玉米素核糖核苷(CZR)、赤霉素3(GA3)、反式玉米素(ZT)、吲哚-3-乙酸(IAA)及脱落酸(ABA)含量,并分析了这5种内源激素与胚珠膨大发育的关系。结果表明,与对照25 ℃室温培养相比,32 ℃热激处理4 d有利于胚珠的膨大及内源激素的积累,且在胚珠膨大期5种内源激素的变化趋势一致,均为递增。各内源激素间的比例关系处于动态变化中,且在整个膨大期,热激处理下IAA/(ZT+CZR)、IAA/ABA和IAA/GA3均较对照25 ℃室温培养高;胚珠膨大率与内源激素IAA呈极显著正相关,相关系数达0.818,与ZT、CZR和ABA均呈显著正相关,与(ZT+CZR)/GA3呈显著负相关。     

豫医无毛小鼠卵巢观察

为了探讨无毛基因的突变对小鼠卵巢的影响,采用病理组织学和流式细胞术研究豫医无毛小鼠卵巢的形态学以及卵巢颗粒细胞周期和凋亡的变化。结果显示6月龄无毛小鼠卵巢颗粒细胞Ap峰和G0／G1期显著高于2月龄无毛小鼠和2、6月龄昆明小鼠。该研究表明,无毛基因的突变诱发了小鼠卵巢颗粒细胞的凋亡,雌性突变小鼠怀孕困难。     

Streptococcus suis type 2 infections in pigs in the Netherlands (part two)

Summary

Since 1983 some pig breeding and fattening farms in the Netherlands have been faced with a considerable mortality in pigs due to Streptococcus suis type 2 infections. The most predominant clinical feature of S. suis type 2 infection is meningitis, although sudden deaths often occur. It was noted that some affected farms had imported breeding stock from the United Kingdom.

Tonsils of slaughter pigs were collected from herds with and without a history of S. suis type 2 infections. Bacteriological examination was done by using an elective‐selective medium. No significant difference was found in carrier rates of S. suis type 2 between clinically healthy and affected herds (38% vs. 45%).

A cohort study was carried out by regular bacteriological examination of tonsil biopsies on a farm with a high incidence of streptococcal meningitis. Twenty‐seven percent of the pigs were carriers of S. suis type 2 at nine weeks of age. Possible methods for disease control are discussed.     

玉米赤霉烯酮中毒大鼠卵巢组织Caspase-3和Caspase-8的表达

10周龄SD大鼠单剂量腹腔注射5 mg/kg玉米赤霉烯酮玉米油溶液,分别于3、6、12、24、48、72、96 h时剖杀取卵巢组织,检测不同时间卵巢组织Caspase-3和Caspase-8的表达.病理组织学观察发现,卵巢组织出现不同程度损伤,卵泡颗粒细胞发生凋亡.免疫组织化学超敏PV法结果表明,试验组与对照组卵巢组织中均有Caspase-3和Caspase-8的表达,并且随着时间的推进呈现动态变化.Caspase-3和Caspase-8试验组表达量均高于对照组.试验组卵巢组织中Caspase-3在3～96 h时表达量均比较高,与对照组相比较差异显著(P＜0.05),且3、6、12、24、48 h的表达量均高于72 h和96 h的表达量.6、12、72、96 h卵巢组织中Caspase-8表达量与对照组相比较差异显著(P＜0.05),3、24、48 h时与对照组相比差异不显著(P＞0.05),且3、6、12、24、48 h的表达量均高于72 h和96 h的表达量.结果表明,大鼠玉米赤霉烯酮中毒可引起卵巢组织的病变及颗粒细胞凋亡,且Caspase-3和Caspase-8在玉米赤霉烯酮中毒大鼠卵巢颗粒细胞凋亡中起着重要作用,进一步证明本试验中死亡受体途径的激活可能参与凋亡的发生.Caspase-8表达量在个别时间段与对照组差异不显著还有待于进一步深入研究.     

Bilateral ovarian adenocarcinoma in a mare causing haemoperitoneum and colic

Abstract

CASE HISTORY: A 13-year-old Thoroughbred mare was presented with a history of mild colic over 3 days. This colic had acutely exacerbated and was unresponsive to analgesic treatment, and was referred to Massey University Veterinary Teaching Hospital.

CLINICAL FINDINGS: On examination the heart rate was 100 beats per minute, and mucous membranes were pale and tacky. A large mass was detected on transrectal palpation in the caudal abdomen to the left of midline. Explorative laparotomy revealed severe haemoperitoneum and several masses that were associated with the reproductive tract. The mare was then subject to euthanasia. On post-mortem examination, adjacent and attached to each ovary were soft, lobulated dark red masses up to 200 mm in diameter. Similar masses were present in the omentum and on the peritoneal surface of the diaphragm and the serosa of the spleen and liver. Histopathology revealed that the neoplastic component of the masses comprised proliferating cuboidal to columnar cells forming disorganised acini and cords separated by dense collagenous stroma. Immunohistochemistry showed the neoplastic cells were positive for cytokeratin AE1/AE3 and vimentin, but negative for cytokeratin 7 and inhibin α.

DIAGNOSIS: Bilateral ovarian adenocarcinoma with transcoelomic metastasis and terminal decompensation due to rupture of a neoplastic mass and consequent haemoperitoneum.

CLINICAL RELEVANCE: To the authors' knowledge, bilateral ovarian adenocarcinoma has not been previously reported in a horse. Ovarian adenocarcinoma should be considered when horses present with haemoperitoneum and colic. Further research is required on the immunohistochemical differentiation of adenocarcinoma of ovarian and intestinal origin in the horse.     

Serological evidence of bovine herpesvirus 2 in northern New Zealand

In the pathological collection of native fauna diseases held at the Taronga Zoo, Sydney, there are materials from about 300 wild, free range brush-tail possums from the Sydney area. Thirty-five of these have neurologic lesions.     

日粮能量水平对蛋鸡性成熟过程中卵巢重和E_2及P_4水平的影响

本文以研究日粮能量水平对蛋鸡性成熟过程中卵巢重、E2及P4水平的影响为目的。结果表明:在性成熟过程中,卵巢重和卵巢表面卵泡数随着年龄和能量水平的提高而增多,22周龄时,除低能Ⅱ组外,其他各组卵巢质量和优势卵泡(10mm以上)明显增多,与低能Ⅱ组差异达显著水平;血清E2和P4含量持续上升,22周龄时均达较高水平;高能组E2和P4水平显著高于低能组,到22周龄,对照组E2水平显著高于其他各组,高能组又显著高于低能Ⅱ组;其他4组P4水平显著高于低能Ⅱ组,说明过低的能量水平通过影响E2和P4水平的分泌进而影响卵巢卵泡的发育。     

Dynamics of apoptosis-related gene expression during follicular development in yak

The potential reproduction power of domestic animals is limited by a complicated follicular atresia process. P53, caspase-9 (Casp9), Bax, Bcl-2 and Fas play a crucial role in the ovarian mitochondrion-dependent apoptosis and death receptor pathway. In accordance with this study, the expression levels of Casp9, Bax, Bcl-2 and Fas were analysed in ovaries and oviducts of yak by immunohistochemistry (IHC). P53 and the above in ovarian granulosa cells (GCs) from atretic (3–6 mm) to healthy follicles (6–8 mm) and in oviducts were examined from the luteal phase to the follicular phase during the oestrous circle by Western blot (WB) and real-time PCR (RT-PCR). Results demonstrated that typical classic apoptotic factors Casp9, Bax, Bcl-2 and Fas were expressed in the cytoplasm and zonal pellucida of oocytes, primordial follicles, primary follicles, ovarian surface epithelium, ovarian GCs, granular lutein cells, surface epithelia in oviduct uterotubal junction and oviduct ampulla during the luteal phase. RT-PCR and WB revealed that P53 and Fas significantly increased in GCs of atretic follicles. P53 and Casp9 increased in oviduct epithelium during the luteal phase, but Fas was unchanged. A contrary tendency was noted in Bcl-2 and Bax expression. Overall, P53 and Fas play an essential role in inducing GC apoptosis, and Bax, Bcl-2, Casp9 and P53 are involved in oviduct epithelial regeneration in yak.     

Effect of genistein on IGF‐1 and IGFBP‐1 in young and aged female rat ovary

The objective of this study was to assess the effects of genistein (GEN) on expression of insulin‐like growth factor 1 (IGF‐1) and insulin‐like growth factor binding protein 1 (IGFBP‐1) in young and aged rat ovary. Forty young female Sprague Dawley (SD) rats (200 ± 20 g) and forty aged female SD rats (490 ± 20 g) were selected and according to weight, they were divided into the following five groups with eight animals in each: negative control group (NC), low‐dose group (L), middle‐dose group (M), high‐dose group (H) and positive control group (PC). GEN group received GEN of 15, 30, 60 mg/kg respectively. It lasted 30 days. Concentrations of serum hormones, IGF‐1 and IGFBP‐1 were determined by enzyme‐linked immunosorbent assay (ELISA). Gene and protein expressions of IGF‐1 and IGFBP‐1 were determined by real‐time PCR and Western blot respectively. Compared with NC, GEN significantly increased oestradiol‐17β(E₂) level in aged rat, reduced luteinizing hormone (LH) level in young and aged rat. Serum levels of IGFBP‐1 in young rats were significantly higher in GEN groups (p < 0.05). mRNA and protein expression levels of IGF‐1 and IGFBP‐1 were positively correlated with GEN dose. GEN could significantly reduce the ratio of IGF‐1/IGFBP‐1 of aged rats. Multivariate Cox regression analysis result showed IGF‐1 and IGFBP‐1 levels significantly correlated with GEN dose. We speculate that there is an association between the addition of GEN and expression of IGF‐1 and IGFBP‐1, and the relationship between them is different in young and aged rat.