多氯联苯对剑尾鱼Na^＋／K^＋—ATPase活性的影响

采用毒性实验的方法，定量地研究了多氯联苯暴露对剑尾鱼肝脏、卵巢及鳃组织中Na^ ／K^ —ATPase活性的影响。结果表明：不同组织其Na^ ／K —ATPase活性的高低存在明显差异。多氯联苯对剑尾鱼肝脏及卵巢的Na^ ／K^ —ATPase活性有抑制作用但不显著；对鳃的Na^ ／K^ —ATPase活性则显示有显著的抑制作用，并随暴露浓度和时间的增加而提高。3种组织中鳃Na^ ／K^ —ATPase对多氯联苯显得更为敏感。     

Influence of Enriched Live Prey and Other Artificial Diets on RNA and DNA Concentration in the Ovary of Tiger Prawn,Penaeus monodon

Abstract

Separate batches of tiger prawn, Penaeus monodon, were fed three different diets, and the effect of these nutritional treatments on RNA and DNA concentrations in the ovary was examined. The given diets did not produce any significant difference in the concentrations of the nucleic acids. DNA remained remarkably stable. RNA/DNA ratio varied from 2.18 to 3.62 and largely followed the pattern similar to that of RNA. Differences in the RNA/DNA ratio did not suggest actual quantitative change in DNA per cell. Cause and effect relations need to be differentiated by analysis of nucleic acid contents at cell level for further insights into the possible link between the dynamics of change in the nucleic acids and broodstock nourishment.     

山羊卵巢无腔卵泡的分离及其形态特征

采用胶原蛋白酶和ＤＮＡ酶结合机械处理分离１～６０日龄山羊卵巢无腔卵泡,每只卵巢平均回收具有３～４层颗粒细胞的可利用无腔卵泡１８．１４枚。在所分离的卵泡中,原始卵泡和初级卵泡占多数,次级卵泡占一小部分。从大于１０日龄山羊卵巢分离的大无腔卵泡的回收率高。     

出雏至性成熟绍鸭卵巢FSH-R与LH-R mRNA表达的变化

用反转录多聚酶链式反应 (RT PCR)方法检测绍鸭出雏至 90日龄卵巢卵泡刺激素受体 (FSH R)和黄体生成素受体 (LH R)mRNA含量的变化。结果表明 ,卵巢FSH RmRNA水平在 30和 6 0日龄较高 ,且 30日龄时极显著高于 1和 90日龄 (P <0 0 1) ,与该阶段较高的卵巢相对生长速度相对应 ;而卵巢LH RmRNA水平随日龄逐渐升高 ,30、 6 0和 90日龄极显著高于 1日龄 (P <0 0 1) ,90日龄显著高于 30和 6 0日龄 (P <0 0 5 )。提示 :卵巢在早期发育阶段 ,尤其是快速增重期 ,对FSH的反应性较高 ;而接近性成熟时 ,可能由于卵泡发育的关系而对LH的反应性升高。     

牦牛NGF基因克隆及其在生殖器官中的表达定位

旨在探究NGF基因的生物学特性及其在母牦牛生殖器官中的表达特性.本研究收集黄体期母牦牛的心、肝、脾、肺、肾以及胎牛期、卵泡期、黄体期、妊娠期母牦牛的卵巢、子宫和输卵管(n=3),利用RT-PCR克隆牦牛NGF基因,并对其序列进行生物信息学分析,利用RT-qPCR技术分析NGF基因的组织表达特性,利用免疫组化技术(IHC...     

绵羊发情周期卵巢微血管密度的观察

采用S-P免疫组织化学法通过CD34抗体标记血管内皮细胞,测定绵羊发情周期的0、5、9、12、15d的卵巢内原始卵泡、初级卵泡、次级卵泡、近成熟卵泡、卵巢间质微血管的分布,并分析微血管密度（MVD）的变化规律,探讨绵羊发情周期不同时期各级卵泡微血管生成状态及卵泡微血管生成与卵泡发育的关系。结果表明,绵羊的原始卵泡周围无独立血管网,而初级卵泡的卵泡膜附近开始出现微血管,其MVD值为3.60±0.89,次级卵泡周围微血管密度值显著增高（P〈0.05）,MVD值为6.80±0.84。大窦腔卵泡0、5、9、12、15dMVD分别为15.80±0.84、23.00±2.30、22.40±2.41、21.20±2.28、34.80±2.39。0～5dMVD值显著升高（P〈0.05）,而5、9、12dMVD值差异均不显著（P〉0.05）,12～15dMVD值又明显升高（P〈0.05）。卵巢间质MVD值在各个发情周期各个时期差异均不显著（P〉0.05）。说明绵羊在发情周期内卵泡的血管新生是从初级卵泡开始的,并且卵巢的血管新生主要发生在卵泡上,而卵巢间质无血管新生现象。     

Intra-abdominal mass aspirate from an alopecic dog

An 11-year-old female Yorkshire terrier was presented to the University of Minnesota Veterinary Medical Center for evaluation of a palpable intra-abdominal mass and alopecia. Abdominal ultrasonography revealed a large, complex, cavitary mass in the left caudal region of the abdomen. A fine needle aspirate of the mass was collected. A population of markedly pleomorphic, large, round to polygonal cells were found singly and in small noncohesive aggregates. The cells contained scant, clear to blue-gray cytoplasm, large, round to oval nuclei, and distinctly stippled to reticular chromatin. Cytologic findings were consistent with a tumor of ovarian origin, with a primary differential diagnosis of germ cell tumor. Hormonal analysis of serum revealed a marked increase in 14-OH-progesterone concentration (2.71 ng/mL, reference interval 0.05-0.69 ng/mL). Ovariohysterectomy was performed, and the mass was found to be in the area of the left ovary. Histologic evaluation of the reproductive tract confirmed a diagnosis of left ovarian dysgerminoma. Based on immunohistochemical stains, the tumor was negative for c-kit (CD117c) and single cells were positive for neuron-specific enolase. A right ovarian cyst and squamous metaplasia of the right uterine horn also were diagnosed. The cyst was presumed to be the source of 14-OH-progesterone, which likely resulted in the squamous metaplasia and dermatopathy. Three months after surgery, the progesterone concentration had returned to normal and the alopecia had nearly resolved. Dysgerminomas in dogs are reported rarely, but have a distinctive, recognizable, cytologic appearance and should be included in the differential diagnosis of an intra-abdominal mass in a reproductively intact female dog.     

平衡阴阳法对多囊卵巢综合征雌雄激素的调节

本文依据月经周期节律演变和阴阳消长变化的规律,在中医基础理论的指导下,用阴阳失衡来阐释多囊卵巢综合征（polycystic ovary syndrome,PCOS）女性周期节律失序导致高雄、低雌激素血症内分泌失调的机制;并遵循《内经》“谨察阴阳所在而调之,以平为期”的宗旨,说明“阴平阳秘”可使PCOS患者达到阴阳平衡及升雌降雄,最终恢复月经周期自然节律的目的。     

东海三疣梭子蟹成熟群体生殖特征和条件状况

为了探求东海三疣梭子蟹成熟群体的发育生殖机制,采用生态学、资源生物学等相关研究方法,通过对三疣梭子蟹性腺、肝胰腺及条件指数等指标的研究,初步建立起雄性三疣梭子蟹成熟阶段发育分期的特征变化,反映了目前东海三疣梭子蟹成熟群体的生殖特征和条件状况。三疣梭子蟹生殖群体由1龄蟹为主,规格偏小。头胸甲宽主要分布在100~160 mm之间,体重为50~200 g。头胸甲宽与长呈线性相关,而头胸甲宽、长与体重则呈幂函数关系。成熟阶段输精管发育程度和头胸甲宽正显著相关。根据三疣梭子蟹输精管的粗糙程度、管径及管重大小,本文首次提出把处于成熟阶段的雄蟹分为5个阶段。输精管重增长为幂函数方式,输精管指数则随发育线性增长。雌蟹卵巢发育规律和头胸甲宽之间关系不甚明显,而和季节相关。研究表明,9月份三疣梭子蟹交配后卵巢迅速发育,增长明显,10~12月份生殖群体卵巢普遍处于Ⅱ~Ⅳ期。三疣梭子蟹肝胰腺重量随体重和甲壳的增长而增加,而肝体指数相反。雌蟹卵巢Ⅱ期阶段梭子蟹肝胰腺重量最高,平均重量为18.21±5.68 g,随后逐渐降低,说明肝胰腺能量发生了转移;相比于雄蟹,雌蟹储能更多。雌蟹卵巢重量随肝胰腺重量变化呈对数关系增长,而雄蟹输精管重随肝胰腺重呈线性。三疣梭子蟹生殖群体在发育变化过程中两个条件指数基本处于一个平衡阶段,雌雄间差异变化不大。