白菜×白芥属间杂种子房培养技术研究

不同植物激素浓度与组合，接种时杂种子房发育天数及子房不同花器附属物对白菜×白芥属间杂种离体子房发育的影响进行了试验研究。结果表明，激素对白菜×白芥杂种种子的形成并非必要条件，然而在Ｗｈｉｔｅ培养基添加适宜的生长素，对杂种子房的发育、杂种种子的产生有明显的促进作用，其中以１．５～２．０ｍｇ／ＬＩＡＡ最为明显。细胞分裂素对杂种种子的产生有抑制作用，浓度愈高，抑制作用愈大；授粉后６～８天的杂种子房培养效果最好；子房花器附属物对杂种种子产生并无明显作用，但携带萼片的子房培养可明显促进子房的生长发育。     

牛卵巢黄体类型与卵泡生长发育关系的组织学研究

本试验通过对牛卵巢不同黄体类型和各级卵泡生长发育状况的组织学观察 ,探讨了不同黄体类型对各级卵泡生长发育的影响 试验结果表明 :1)不同类型黄体之间 ,黄体功能的强弱不同 黄体功能由强至弱依次为圆锥状型 (Ⅰ型 )、火山口状型 (Ⅱ型 )、蘑菇状型 (Ⅲ型 )、扁平片状型 (Ⅳ型 )、表面无黄体型 (Ⅴ型 ) 2 )黄体类型对生长发育过程中各级卵泡的数量有一定的影响 Ⅴ型腔前卵泡总数和有腔卵泡总数与Ⅰ、Ⅱ、Ⅲ、Ⅳ型的腔前卵泡总数和有腔卵泡总数之间差异极显著 (p <0 0 1) ,而Ⅰ、Ⅱ型之间差异不显著 Ⅲ、Ⅳ之间差异显著 ,Ⅰ、Ⅱ型与Ⅲ、Ⅳ型之间差异显著 (p <0 0 5) 3)黄体类型对各级卵泡平均直径均无显著影响     

葛根水煎液对未性成熟小白鼠子宫和卵巢组织形态结构的影响

选取21日龄的未性成熟雌小白鼠14只,体重为19.13±2.23 g,各窝随机分为对照组和实验组.给实验组灌胃葛根水煎液0.25 ml,相当于每毫升含生药0.5 g;对照组灌胃蒸馏水0.25 ml ,其他条件均一致.每组每天灌药或蒸馏水一次,连续16 d,停药后切断颈动脉将小白鼠处死,剖腹取出子宫、卵巢并称重.结果证明,葛根水煎液对小白鼠的体重没有明显的增重作用,但对小白鼠子宫、卵巢有一定的增重作用.在组织形态学上,葛根水煎液对小白鼠的子宫腺体数量和卵巢的卵泡数量有明显增多作用.结果表明,葛根能促进未性成熟小白鼠的子宫、卵巢的发育.     

皖西白鹅不同时期卵巢肥大细胞的比较研究

应用组织化学甲苯胺蓝染液染色皖西白鹅不同时期卵巢的肥大细胞(MC)显微镜观察测量后,对数据进行统计和方差分析。结果表明:皖西白鹅产蛋期与就巢期MC的形态、大小及数量差异极显著(P<0.01),产蛋前与就巢期差异显著(P<0.05),而产蛋期与产蛋前差异不显著(P>0.05)。说明同种皖西白鹅的卵巢MC,在不同时期存在着差异性。     

九个粳稻品种未传粉子房的培养

9个粳稻品种未传粉子房的培养试验表明,品种间愈伤组织的诱导率、分化率均存在差异。早粳优于晚梗。抽穗期早的品种具有较高的成苗率。外源激素对诱导分化具有十分重要的作用。MCPA(2-甲基-4-氯苯氧乙酸)优于2.4-D。     

Serum Profiles of Luteinizing Hormone,Oestradiol and Cholesterol and Ovarian Functions in Layer Poultry Birds (Gallus domesticus) Fed Diets Containing Furazolidone

This study was carried out to assess the serum profiles of luteinizing hormone (LH), oestradiol, cholesterol and ovarian functions in layer poultry birds (Rhode Island Red: Gallus domesticus) fed a diet containing various concentrations of furazolidone (FZ). A total of 40 birds were randomly assigned to receive FZ 0, 200, 400 or 800 mg/kg feed (ppm) daily during the pre-laying age, i.e. 13-18 weeks (for 5 weeks). Blood samples were collected at weekly intervals. Concentrations of LH and oestradiol in serum were estimated at alternate weeks using radioimmunoassays. Serum cholesterol levels were analysed by an enzymatic calorimetric method. Furazolidone administration was terminated at the 18th week of age. The birds were sacrificed at 22nd week of age and ovarian tissues were processed for morphometric studies. Serum LH, oestradiol and cholesterol levels were affected by age (p < 0.001) and FZ dose (p < 0.001). Serum LH and oestradiol levels were lower (p < 0.05) in birds receiving FZ 800 mg/kg feed daily compared with the controls, whereas serum cholesterol profiles were lower (p < 0.05) in all FZ-administered groups than in the control group. The mean weight of ovaries having no yolky follicles observed in the group receiving FZ 400 or 800 mg/kg feed per day was reduced (p < 0.05) compared with the control group. Dosing FZ at 800 mg/kg feed per day reduced (p < 0.05) the mean volume of ovaries having no yolky follicles compared with the control group. In birds receiving FZ 800 mg/kg feed per day, the mean length of the oviduct was reduced (p < 0.05) as compared with the control group. Morphometric studies revealed that the mean number of oocytes with diameter in the range 401-800 microm decreased (p < 0.05) in birds fed FZ 400 or 800 mg/kg feed per day. Initial egg production was affected by age (p < 0.001) and dose (p < 0.001) of FZ. The mean number of eggs laid by different groups revealed that egg production was reduced (p < 0.05) in birds receiving FZ 800 mg/kg feed per day as compared with the controls. The present data suggest that FZ causes suppression in serum profiles of LH, oestradiol, cholesterol and ovarian functions in Rhode Island Red layer poultry birds. Therefore, great care must be taken with use of FZ in layer poultry birds (Gallius domesticus) with regard to dosage and duration of administration.     

卵泡抑制素与GFP融合基因疫苗免疫对大鼠卵巢和生殖激素的影响

将50只大鼠随机分为5组,分别注射0.2（T1）、0.6（T2）、1g/L（T3）pEGISI,100μg空载体pEGFP-N1（C1）和100μL生理盐水（C2）,以探讨在没有使用免疫佐剂的情况下,抑制素与GFP融合基因疫苗pEGISI免疫对大鼠卵巢和生殖激素的影响。研究结果,加强免疫能提高抗体P/N值,T3组在加强免疫期与对照组差异显著（P〈0.05）;加强免疫第2周时T3组抗体阳性鼠比例达40%,与T1和T2组差异均显著（P〈0.05）。T3组卵巢大小和卵泡数均显著高于对照组（P〈0.05）,但抗体阳性鼠大卵泡数与阴性鼠差异不显著（P〉0.05）。各剂量组促卵泡素和雌二醇均高于对照组,且在加强免疫第2周时T3组与对照组差异显著（P〈0.05）;各剂量组孕酮含量与对照组差异均不显著（P〉0.05）。结果表明,在没有使用免疫佐剂的前提下,抑制素pEGISI基因免疫可产生抗体,促进卵巢发育和FSH分泌。本试验条件下100μg是最佳免疫剂量。     

6月龄和成年牦牛卵巢及卵泡发育状况研究

为了研究6月龄牦牛和成年牦牛卵巢及表面卵泡发育状况,试验比较了6月龄和成年牦牛卵巢长度、宽度、厚度、重量、卵泡数量以及卵母细胞体外成熟培养效果。结果表明:成年牦牛卵巢长度(2.29±0.43)cm、宽度(1.91±1.31)cm和厚度(1.60±1.90)cm均显著大于6月龄牦牛[(1.65±0.30)cm、(1.14±0.25)cm、(0.79±0.26)cm](P<0.05),成年牦牛卵巢体积(6.92±7.00)cm3和重量(3.19±1.58)g极显著大于6月龄牦牛体积(1.63±0.93)cm3和重量(0.87±0.44)g(P<0.01)。6月龄牦牛Ⅰ级卵泡数(14.47±8.74)枚和平均总卵泡数(15.17±8.87)枚极显著高于成年牦牛Ⅰ级卵泡数(7.97±3.72)枚和平均总卵泡数(8.98±3.87)枚(P<0.01),Ⅱ级卵泡数差异不显著(P>0.05),成年牦牛平均每头含(0.02±0.15)枚Ⅲ级卵泡,而6月龄牦牛无Ⅲ级卵泡。成年牦牛有黄体卵巢重量显著大于无黄体卵巢重量(P<0.05),有黄体卵巢含(0.06±0.24)枚Ⅲ级卵泡,而无黄体卵巢不含Ⅲ级卵泡。6月龄和成年牦牛A、B级卵母细胞体外培养成熟率分别为(81.39±3.53)%、(80.44±4.50)%,差异不显著(P>0.05),而6月龄牦牛卵巢的平均卵母细胞数和平均A、B级卵母细胞数均显著高于成年牦牛(P<0.05)。     

一例犬卵巢纤维肉瘤的病理学诊断

某警犬基地一繁育种犬于2011年3月18日死亡,根据发病及死亡经过、尸体剖检和病理学诊断,结果为卵巢纤维肉瘤并发肺转移,同时对病因进行分析。     

Histological Study on the Polyovular Folliclular Development of Chinese Prematural Lubei White Goat

The present study was conducted to investigate the histological characteristics of Polyovular follicles in Chinese Lubei white female goats with age 2-5 months. The results suggested that the healthy antral follicles with twin-oocyte could be observed. There were many multi-oocyte phenomena in primordial and primary follicles with a proportion of nearly 75% in primordial follicles. Primordial follicle was in the outer of cortex in ovaries and most of them remained active. The nest of multi-nuclear primordial follicle was developing into primordial follicle with 1 to 3 nuclei.