人参果皂苷口服液质量标准研究

通过对人参果皂苷口服液质量进行研究，建立人参果皂苷口服液质量标准。利用薄层检查对其鉴定。采用分光光度法及滴定法对人参果皂苷口服液中人参皂苷含量和木糖醇、山梨酸含量进行测定。结果表明人参果皂苷口服液质量检验方法简便、准确，可用于快速检验人参果皂苷口服液的质量。     

散发性结直肠癌遗传标识物研究进展

散发性结直肠癌（sporadic colorectal cancer,sCRC）是一类没有明显家族遗传倾向,由遗传因素和环境因素共同作用的消化道恶性肿瘤。近年来,分子技术的发展使发现复杂疾病潜在的生物标识物成为可能。基于全基因组关联研究（genome-wideassociationstudy,GWAS）以及高通量测序技术的应用,已经从全基因组范围内确定了大量肿瘤易感基因以及其后天突变标识物。本文对近年来散发性结直肠癌（sCRC）遗传标识物最新研究进展作一综述,并对其未来研究方向加以展望。     

CYP1A1基因MspⅠ多态性与卵巢癌易感性关系的研究

目的探讨张家口地区卵巢癌易感性与CYP1A1基因MspⅠ位点多态性的关系。方法研究采用PCR-RFLP技术,将张家口地区34例卵巢癌患者作为实验组,45例健康女性人群作为对照组,分析CYP1A1基因3’端限制性内切酶MspⅠ位点基因的基因多态性。结果卵巢癌组MspⅠ基因型分布为：基因型TT占21．8％;基因型TC占52．7％;基因型CC占25．5％;等位基因T、C分别为48．2％、51．8％。健康人群组MspⅠ基因型分布为,基因型TT占42．2％;基因型TC占46．7％;基因型CC占11．1％;等位基因T、C分别为65．6％、34．4％。结论该地区健康人群组和卵巢癌组CYP1A1基因MspⅠ位点均呈多态性分布,两者之间差异有显著性（P〈0．05）,提示卵巢癌发病率可能与CYP1A1基因型有关。     

平鲉微杯虫的扫描电镜观察

扫描电镜观察平鲉微杯虫（Microcotyle sebastisci Yamaguti，1958）显示：该虫中型个体，具口吸盘1对，口吸盘之间可见口孔，具横隔。口孔正下方为一圆形生殖孔，卵纺锤形具双极丝。后吸器鸡冠状，上面对称排列着两行无柄微杯型吸铗，吸铗形状、大小相近。体表具横向皱褶，背面体表具乳头状乳突，腹面体表密披杆状纤毛。[中国水产科学，2006，13（4）：511—516]     

miR-142-3p在MCF-7细胞中靶向调节AKT pathway活性机理

为探究miR-142-3p在MCF-7细胞中作用机理,采用RNA免疫共沉淀技术和双荧光素酶报告基因技术筛选及验证miR-142-3p作用靶基因;蛋白质免疫印迹技术验证靶基因及其介导的PI3K-AKT-mTOR信号通路蛋白表达量及通路活性;应用实时荧光定量PCR验证靶基因PTEN对miR-142-3p调节关系。结果表明,miR-142-3p靶向调节AKT和PTEN表达;miR-142-3p过表达组中PI3K-AKT-mTOR通路活性显著降低;miR-142-3p抑制组中PI3K-AKT-mTOR通路活性显著上升;抑制PTEN表达显著提高miR-142-3p表达量。因此miR-142-3p靶向调节AKT和PTEN表达继而抑制PI3K-AKT-mTOR信号通路活性,PTEN与miR-142-3p存在调节关系。     

Effect of anaesthesia on cell-mediated immunity in dogs undergoing mastectomy for mammary cancer

ObjectiveTo investigate if anaesthesia for canine cancer mastectomy further influences host cell-mediated immunity (CMI) promoting cancer progression.Study designA randomized, controlled, blinded clinical study.AnimalsA total of 20 bitches with malignant mammary tumours of clinical stage II or III undergoing the same type of mastectomy (regional mastectomy).MethodsDogs were randomly allocated to one of two anaesthetic groups (10 per group). The anaesthetic protocol of group A used minimally immunosuppressive drugs (tramadol, robenacoxib, propofol), whereas that of group B (control) used more immunosuppressive drugs (morphine, fentanyl, thiopental, isoflurane). For each animal, measurements of white blood cells (WBCs), neutrophils and lymphocytes, and flow cytometric assessment of T cells (CD3⁺), helper T cells (CD4⁺), cytotoxic T cells (CD8⁺) and CD5^low+ T cells were performed prior to anaesthesia (day 0) and on days 3 and 10 postsurgery. Data were analysed using a General Linear Model for repeated measures and presented as mean ± standard deviation, p ≤ 0.05.ResultsIn all animals, on day 3, WBCs and neutrophils were significantly increased (p < 0.0005), while flow cytometry revealed significantly decreased relative percentages of T cells (CD3⁺) (p = 0.003) and their subpopulations CD4⁺ (p = 0.006), CD8⁺ (p = 0.029) and CD5^low+ (p = 0.031). Specifically, on day 3, the cytotoxic T cells (CD8⁺) were significantly decreased (p = 0.05) only in group B, whereas the CD4⁺ (p = 0.006) and CD5^low+ (p = 0.008) T cells in group A. The only significant difference between groups was found preoperatively in the CD4⁺/CD8⁺ ratio, which was higher in group A (p = 0.006).Conclusions and clinical relevanceIn dogs with mammary cancer undergoing regional mastectomy, a significant decrease in components of CMI was observed on day 3 postsurgery in both anaesthetic groups. Some indication, however, for better preserved cellular immunity by less immunosuppressive anaesthetic/analgesic drugs was detected, rendering their use advisable.     

Phase I lead‐in and subsequent randomized trial assessing safety and modulation of regulatory T cell numbers following a maximally tolerated dose doxorubicin and metronomic dose cyclophosphamide combination chemotherapy protocol in tumour‐bearing dogs

Maximally tolerated dose (MTD) and metronomic dose chemotherapeutic approaches alter the immune system and the angiogenic process in different yet potentially complementary ways. A combination of MTD doxorubicin (MTD‐DOX) and metronomic cyclophosphamide (mCTX) protocol was evaluated for safety and effect on circulating regulatory T (Treg) cells. We found that mCTX can be safely administered with MTD‐DOX in tumour‐bearing dogs. Both combination DOX/mCTX and single‐agent DOX resulted in significant depletions of circulating lymphocytes throughout the chemotherapy cycle without apparent selectivity for Tregs. The indiscriminant lymphocyte depletions were similar between dogs randomized to receive DOX and dogs randomized to receive DOX/mCTX, suggesting this effect is because of DOX alone. These findings may have implications as to the therapeutic benefit (or lack thereof) of concurrent combination MTD and metronomic protocols. Future investigations are required to determine the effects and indeed the efficacy of concurrent versus sequential applications of MTD and metronomic chemotherapy protocols.     

Research Progress of Matrine and Oxymatirne in the Anti-tumor Mechanism

The Matrine and Oxymatirne are two kinds of alkaloid, and have many kinds of pharmacological functions. In recent years, their anti-tumor mechanism has been paid more attention. This article is a summarization of the research progress of Matrine and Oxymatirne in the anti-tumor mechanism. Now people have found out that the Matrine and Oxymatirne can restrain the multiplication of tumor cells via restrainning the synthesizatinon of DNA and the enzyme activity and effectting the normal cycle, and they also can restrain the transfer of the tumour via controlling the expression of the genes, and induce the death of the tumour cell and induce the tumour cell to differentiate into the common cell via controlling the expression of the genes and effecting the telomerase activity.     

奥唑嗪盐酸口服液的药理学实验研究

目的通过对奥唑嗪（AZQ）口服液初步的药理学实验观察,确定其对支气管哮喘的治疗作用．方法采用药理学实验方法,将200只小鼠随机分成4组,每组50只,各组分别设置中药奥唑嗪大剂量组（200mg／kg）、中剂量组（100mg／kg）、小剂量组（50ms／kg）3个剂量组、阳性对照组（抗炎为醋酸可的松;止咳为蛇胆川贝液;祛痰为氯化铵;平喘为氨茶碱）及生理盐水对照组,分别对其进行抗炎、止咳、祛痰、平喘等药效学观察．结果奥唑嗪口服液100mg／kg和50mg／kg剂量组对抗炎有一定作用;50mg／kg剂量组对止咳有显著作用;70mg／kg和100mg／kg剂量组对祛痰有显著疗效;100mg／kg和50mg／kg剂量组对平喘有明显作用．结论奥唑嗪口服液对抗炎、止咳、祛痰、平喘有一定疗效,可以开发研制为二类新药．