美味牛肝菌中油脂提取工艺的研究

采用超声波辅助法提取美味牛肝菌中的油脂，在单因素（提取时间、液料比和温度）实验考察的基础上，以油脂提取率为指标，通过响应面法对美味牛肝菌油脂的提取工艺进行优化。实验结果表明：响应面回归模型显著，拟合性好，可用于预测油脂提取率；优化后的油脂提取工艺为：液料比34.2 mL/g，温度36℃，提取时间4.5 h，此条件下油脂提取率为3.54826 %，与模型预测值相符，表明优化的油脂提取工艺合理。     

温莪术油提取方法优化研究

[目的]为克服现有提取技术存在的提取率低、提取时间长等不足,优化得到温莪术中莪术油的高效提取方法。[方法]以莪术油的提取率为指标,考察有机溶剂、超声时间、料液比、提取时间等因素对其提取率的影响。[结果]采用超声波前处理-微波法,莪术油的提取率可达4.71%,均高于超临界CO2萃取和水蒸汽蒸馏法的4.47%和2.53%。[结论]该试验建立的超声法前处理-微波法提取莪术油方法,可为莪术油的工业化生产及后续质量控制等相关研究提供技术支持。     

火灾环境下邻近罐壁热辐射分布实验

油罐火灾蔓延的主要因素是油罐热辐射,在着火罐火焰热辐射作用下,邻近罐极易被引燃,造成整个罐区大面积起火。为了研究邻近罐罐壁热辐射分布规律,建立了油罐燃烧对邻近罐热辐射影响的小尺寸实验装置,开展了火灾环境下邻近罐壁热辐射分布的实验研究。结果表明:在火灾环境下,邻近罐正对着火罐方向的罐壁受辐射作用最大,热辐射从罐顶到罐底逐渐降低、从中心向两边呈轴对称降低;随着L/D(L为相邻两个油罐的距离,D为油罐的直径)增大,邻近罐热辐射下降。实验数据为火灾环境下邻近罐的热辐射研究提供了参考,对防止邻近罐被引燃,做好油罐区火灾爆炸事故的预防具有重大意义。     

美国油气管道基本失效概率评估方法及启示

为充分利用油气管道历史失效数据,减少失效概率评估过程中的主观性,提出油气管道基本失效概率的概念及评估方法。对美国管道及危险物品安全管理局数据库的油气管道里程数据、事故数据及失效因素数据进行全面分析,采用基于事故统计的方法评估油气管道基本失效概率。结果表明:美国危险液体管道、输气集气管道、配气管道发生一般事故的基本失效概率分别为1.29次/(10^3 km·a)、2.17次/(10^4 km·a)及4.08次/(10^5 km·a),发生较大事故的基本失效概率分别为4.58次/(10^4 km·a)、1.41次/(10^4 km·a)及2.38次/(10^5 km·a),发生重大事故的基本失效概率分别为9.09次/(10^6 km·a)、9.79次/(10^6 km·a)及1.11次/(10^5 km·a)。美国基本失效概率可作为油气管道失效概率评估的基准线,也可作为风险可接受标准的依据,但不能直接应用于中国管道风险评价,需对其进行修正,建立适用于中国管道的失效数据库。     

黑豆—油菜与大豆—油菜轮作模式土壤镉修复能力比较

通过设置黑豆-油菜、大豆-油菜的轮作模式在湖南醴陵进行大田试验,结果发现黑豆表现出较强的镉富集能力,其根、茎、叶及果壳中镉含量分别为2.14、2.63、4.10及1.34 mg/kg,富集系数均在1以上。油菜各个部位镉含量较高的分别为叶与茎,达到了7.13和4.73 mg/kg。黑豆各部位镉转运系数均高于大豆,各油料作物提取的重金属主要集中在茎与叶。大豆叶的镉富集系数为2.06,茎的镉富集系数为0.86,明显低于黑豆同部位的富集能力。每公顷土地经过一轮黑豆-油菜、大豆-油菜轮作分别能够从土壤中提取73.52 g和51.68 g的镉,对土壤重金属镉的修复效率约为3%。     

鸡胚及禽类细胞系在生物医药领域的应用及研究进展

鸡胚因发育过程清楚，长久以来作为基础和应用科学研究领域重要的实验模型，尤其在鸡胚发育早期绒毛尿囊膜阶段，因其血管丰富，是天然的免疫缺陷宿主，是病理学、药理学和肿瘤学等研究领域的理想实验模型。作者简述了发育早期的鸡胚组织结构，并介绍了鸡胚绒毛尿囊膜在肿瘤研究、血管生成、器官移植、烧伤等疾病机理研究中的应用，以及在鸡胚病理模型基础上进行的抗肿瘤药物筛选的应用。重点介绍了鸡胚及禽类细胞系在病毒繁殖和疫苗生产、治疗性蛋白和单抗生产方面的应用研究进展。多种人源病毒、禽源病毒、支原体等可在鸡胚及禽类细胞上增殖，并用于疫苗生产。作者对常用的禽类纤维原细胞和多能干细胞的发展和特点进行了阐述，并总结了商业化的禽类细胞系来源以及部分易感病毒。鸡胚表达系统能够在目的蛋白特定位点产生人源化糖基，减少目的蛋白对人的过敏反应，且禽蛋廉价易得，可作为生产人用单克隆抗体和治疗性蛋白的合适供体。作者介绍了鸡胚及禽类细胞系在生物医药领域应用的最新进展，并对鸡胚作为动物模型在未来的应用进行了展望。     

Genetic analysis and QTL mapping of growth period traits and plant height traits in soybean recombinant inbred lines from Dongnong 47 × PI 317334-B

High and stable yield is the main goal of soybean genetic improvement. In this study, association analysis was used to detect the quantitative trait loci (QTL) for the plant height, and soybean growth period using 182 SSR markers in the RIL population of 136 F_4:8 lines, which developed from a cross between photoperiod-insensitive cultivar ‘Dongnong 47’ and photoperiod-sensitive variety PI317334–B. The results showed that 33 QTLs related to soybean growth period and plant height traits were detected by compound interval mapping, and were located on 12 linkage groups including N, C1, C2, J, D1a, B2, E, G, A2, O, L, I, with the contribution rate of 7.85–33.84%. These QTL loci and linkage markers related to soybean photoperiod sensitivity, would be helpful to identify key genes that control soybean photoperiod sensitivity, and provide an important basis for the breeding of new photoperiod-insensitive soybean varieties based on molecular design breeding.     

In vitro susceptibility of Brachyspira hyodysenteriae to organic acids and essential oil components

The antibacterial potential of organic acids and essential oil components against Brachyspira hyodysenteriae, the causative pathogen of swine dysentery, was evaluated. Minimum inhibitory concentrations (MIC) of 15 compounds were determined at pH 7.2 and pH 6.0, using a broth microdilution assay. In addition, possible synergism was determined. MIC values for the three tested strains were similar. For organic acids, MIC values at pH 6.0 were lower than at pH 7.2. B. hyodysenteriae was most sensitive to cinnamaldehyde and lauric acid, with MIC values <1.5 mM. Most antibacterial effects of binary combinations were additive, however, for thymol and carvacrol, synergism could be observed. In vitro results demonstrate the antibacterial action of certain essential oil components and organic acids against B. hyodysenteriae.     

新型国产白油佐剂与进口Marcol-52白油佐剂配制疫苗的比较试验

The Newcastle disease inactivated vaccines were prepared with oil adjuvants respectively from domestic and import.The experiments were done in order to compare the new domestic oil and Marcol-52 according to a series of testing indexes. The results showed that the quality of the new domestic oil was better than Marcol-52, except the absorptive quality.