Design and performance of recirculating aquaculture system for marine finfish broodstock development

Tropical and subtropical climatic conditions in India present an ideal and unique opportunity for being the leader in tropical marine finfish aquaculture. However, the problem persist due to non-availability of marine finfish seed for the culture. In response to this problem, broodstock development of different tropical marine finfishes for seed production was started. The present study was undertaken to design a recirculating aquaculture system (RAS) and studying their performance in managing the various water quality required for the marine finfish broodstock development and breeding. The design of RAS, developed in the present study, included a broodstock tank, egg collection chamber, electrical pump, rapid sand filter, venturi type protein skimmer and biological filter. Two RAS were designed, one was stocked with a demersal fish species, orange spotted grouper (Epinephelus coioides) and the other was stocked with a pelagic fish species, Indian pompano (Trachinotus mookalee) at the rate 1 and 0.5 kg/m³ with a sex ratio of 1:1 and 1:2 (female: male) respectively. Various physio-chemical parameters, viz, total ammonia nitrogen (TAN), nitrite, nitrate, pH, alkalinity, temperature, free carbon dioxide (CO₂) and dissolved oxygen (DO) of both tank water were analyzed to assess the performance of recirculating aquaculture system in maintaining the water quality. Gonadal development of the fishes was assessed and the spawning performance was recorded and finally, economic performance of the system was also evaluated. During the entire experimental period, mean monthly total ammonia nitrogen was less than 0.07 and 0.06 mg L⁻¹ and mean monthly nitrite was less than 0.02 and 0.01 mg L⁻¹ in orange spotted grouper and Indian pompano RAS tanks respectively. The pH (7.8–8.2), DO (>4 mg/L) and alkalinity (100–120 mg/L) were found to be in optimum range in both recirculating aquaculture systems. Carbon dioxide was found to be nil during the entire experimental period in both the systems. In fact these levels were comparable or less than that is reported as the permissible limits for broodstock development. Indian pompano and Orange spotted grouper matured and spawning was obtained with production of fertilized eggs round the year. Economic evaluation showed the price of 10,000 fertilized eggs of orange spotted grouper to be US $ 1.33. The design of RAS devised in the present study is efficient in controlling and maintaining optimum water quality for broodstock development of both demersal and pelagic finfishes. The fishes stocked in RAS attained final maturation and round the year spawning was obtained.     

Effect of the particle size on the ammonia removal rate and the bacterial community composition of bioflocs

The total ammonia nitrogen (TAN) removal efficiency and bacterial community composition of bioflocs with <50-μm particle size, > 50-μm particle size and un-sieved bioflocs were investigated in the current study. The initial ratio of dissolved organic carbon to TAN (DOC/TAN) in the three groups were about 14:1. No significant difference was found in the removal rate of TAN, average concentrations of TAN and nitrite nitrogen among the three groups (P > 0.05). The C/N (w/w) ratio of the > 50-μm bioflocs was significantly higher than those of the other groups. No significant differences were found in the crude protein content in the bioflocs among the three groups. The development of the bacterial community compositions of the bioflocs was analyzed by Illumina MiSeq sequencing analyses. Most OTUs were shared among the three groups at all the sampled time points. With the increase in the relative abundance of phylum Firmicutes, that of phylum Proteobacteria, Chorolexi, and Bacteroidetes decreased in all the three groups. The phylum Firmicutes and genus Bacillus were predominant in all the sampled time points. At the end of the experiment, genus Bacillus accounted for 81% in the < 50-μm group, 82% in the > 50-μm group, and 75% in the un-sieved group.     

EM菌在水产养殖中的应用概述

为了能改善水质、渔业增殖和修复生态环境,EM菌的应用得到了广泛的关注,成为近几年研究的热点。鉴于此,本研究系统梳理了EM菌的定义及来源,综述了国内外近几十年EM菌在水产养殖中的作用效果及其作用机理,阐述了影响EM菌作用效果的因素等;发现EM菌在调控微生物生态结构、降低水环境中有害物质、提高免疫、增重增产等方面有着显著的效果,但EM菌技术的基础理论研究还比较薄弱,且生产技术及应用过程等还存在着不足。因此今后应提高生产技术,建立和提高筛选系统,并开发EM菌在水产养殖研究和应用的新领域,为水产养殖创造更大的经济价值,并为可持续养殖提供可能。     

An investigation of links between metabolic rate and feed efficiency in European sea bass Dicentrarchus labrax

Feed efficiency (FE) is the amount of body weight gain for a given feed intake. Improving FE through selective breeding is key for sustainable finfish aquaculture but its evaluation at individual level is technically challenging. We therefore investigated whether individual routine metabolic rate (RMR) was a predictor of individual FE in the European sea bass Dicentrarchus labrax, a major species in European mariculture. The European sea bass has three genetically distinct populations across its geographical range, namely Atlantic (AT), West Mediterranean (WM), and East Mediterranean (EM). We compared FE and RMR of fish from these three populations at 18 or 24 °C. We held 200 fish (62 AT, 66 WM, and 72 EM) in individual aquaria and fed them from ad libitum down to fasting. FI was assessed for an ad libitum feeding rate and for a fixed restricted ration (1% of metabolic body weight·day⁻¹, with metabolic body weight = body weight^0.8). After being refed 12 wk in a common tank, individual RMR was measured over 36 h by intermittent flow respirometry. There was a significant effect of temperature whereby fish at 18 °C had greater mean FE (P < 0.05) and lower RMR (P < 0.001). There was also a significant effect of population, where AT fish had lower FE (P < 0.05) and greater RMR (P < 0.001) than WM and EM, at both temperatures. Despite these differences in temperature and population means, individual FE and RMR were not significantly correlated (P > 0.05). Therefore, although the results provide evidence of an association between metabolic rate and FE, RMR was not a predictor of individual FE, for reasons that require further investigation.     

浙江水产养殖环境中铬的形态分析和污染情况调查

本文对浙江省池塘养殖、滩涂养殖、浅海筏式养殖、工厂化养殖和网箱养殖等典型养殖模式 环境中铬的污染情况进行抽样调查,根据本单位开发方法对总铬和有效态铬中的两种价态铬进行测 定。结果表明,我省水产养殖环境中,水质中总铬和毒性较大的Cr6+含量较小,均在《生活饮用水卫 生标准》（0.05mg/L）限量标准以下,未发现超出渔业水质标准的情况。     

循环水养殖系统水净化装备技术研究

循环水养殖系统为当前水产养殖先进生产力的发展方向,其具养殖水环境高度可控、产品质量安全可靠、节水、节地等特点,符合国家转变经济增长方式,节能减排的战略需求。循环水养殖水净化装备是循环水养殖系统核心组成。其为循环水养殖生产中的水产养殖对象提供良好水生态环境,提高水产养殖品产量及质量,提升资源利用率。本文以循环水养殖系统水净化典型工艺为主线,对其主要环节包括悬浮物去除、溶解性有机物降解、杀菌消毒、增氧、调温等所涉及的装备技术进行探讨及总结,并提出发展建议,以期为精准、高效、节能循环水养殖系统构建,推进其进一步应用提供支持。     

基于大型渔业平台的深远海渔业发展现状与思考

基于渔业产业现状,分析国内外在大型渔业平台、深海网箱和养殖工船方面的发展现状,提出“游弋式”养殖工船及深远海渔业的基本概念。受限于投资成本、技术发展水平等客观因素,中国深远海养殖发展速度缓慢,但近年来取得了一定的发展成效。笔者针对现阶段渔业粗放型生产方式的现状,从国家战略、渔业改革和产业需求等角度分析深远海渔业发展的必要性,认为发展以养殖工船为代表的深远海大型渔业平台是实现渔业强国之路的有效途径之一,走出一条符合中国国情的深远海渔业平台的开发之路势在必行。     

中国水产养殖污染物排放总量控制框架构建

近10年来,中国水产养殖业快速发展,产量大幅增加,随着绿色养殖概念的提出和总量控制制度的不断完善,水产养殖污染物排放强度明显下降,水产养殖污染物排放总量控制工作已见成效.本研究分析了水产养殖环境污染的产生与排放情况;总结了中国近年来为实现水产养殖污染物排放总量控制目标,在优化养殖布局、控制养殖环境污染、发展健康养殖和开...     

基于Ecopath模型的海水池塘主要养殖模式比较分析

为了揭示台州温岭地区海水池塘不同养殖模式间的物质流动规律,以贝类单养和虾贝综合养殖2种模式的海水池塘为研究对象,基于EWE 6.5软件分别构建了14和15个功能组的Ecopath模型。结果显示,综合养殖模式第Ⅰ、Ⅱ营养级之间物质传输效率(52.1%)高于贝类单养模式(26.5%),同时其碎屑组的物质流通量占比(20.81%)也高于贝类单养模式(4.64%)。与贝类单养模式相比,综合养殖模式关键种从大型浮游动物转为凡纳滨对虾(Litopenaeus vannamei),混合营养效益分析显示虾功能组主要对浮游生物和碎屑产生影响,导致浮游动物生态营养学效率(Ecotrophic efficiency, EE)升高,浮游植物EE值降低,而两种模式中贝类功能组混合营养效益未发生明显改变。贝类单养和虾贝综合养殖模式的Finn’s循环指数分别为2.75%和13.21%,系统连接指数分别为0.256和0.250,杂食指数分别为0.049和0.065,这表明由于综合养殖模式具有更完善的食物网,整体结构要优于单养模式,但两种模式整体成熟度和稳定性均较低,存在较大提升空间,可引入新功能组,以进一步提高系统稳...     

基于轻量级神经网络MobileNetV3-Small的鲈鱼摄食状态分类

在集约化水产养殖过程中,饲料投喂是控制养殖成本,提高养殖效率的关键。室外环境复杂多变且难以控制,适用于此环境的移动设备计算能力较弱,通过识别鱼类摄食状态实现智能投喂仍存在困难。针对此种现象,该研究选取了轻量级神经网络MobileNetV3-Small对鲈鱼摄食状态进行分类。通过水上摄像机采集水面鲈鱼进食图像,根据鲈鱼进食规律选取每轮投喂后第80～110秒的图片建立数据集,经训练后的MobileNetV3-Small网络模型在测试集的准确率达到99.60%,召回率为99.40%,精准率为99.80%,F1分数为99.60%。通过与ResNet-18, ShuffleNetV2和MobileNetV3-Large深度学习模型相比,MobileNetV3-Small模型的计算量最小为582 M,平均分类速率最大为39.21帧/s。与传统机器学习模型KNN（K-Nearest Neighbors）、SVM（Support Vector Machine）、GBDT（Gradient Boosting Decision Tree）和Stacking相比,MobileNetV3-Small模型的综合准确率高出12.74、23.85、3.60和2.78个百分点。为进一步验证该模型有效性,在室外真实养殖环境进行投喂试验。结果显示,与人工投喂相比,基于该分类模型决策的鲈鱼投喂方式的饵料系数为1.42,质量增加率为5.56%。在室外真实养殖环境下,MobileNetV3-Small模型对鲈鱼摄食状态有较好的分类效果,基于该分类模型决策的鲈鱼投喂方式在一定程度上能够代替养殖人员进行决策,为室外集约化养殖环境下的高效智能投喂提供了参考。