拼音首字母排检法的研究与改进

在研究现有拼音首字母排检法的基础上,提出针对多音字、模糊音问题的多重对照表、多字段检索码改进方法,推进拼音首字母排检法的实用进程。     

北京鸭脑桥内3个神经核的形态学研究

采用火棉胶包埋,Nissl（克紫）、Luxol固蓝——克紫、Weil氏染色方法,对9例成年北京鸭外展神经核、外展神经副核和面神经核的形态、位置和细胞构筑进行了研究。计算和测量其中5例北京鸭三核团的长度和核团内细胞脑体的直径。用统计学方法处理,结果如下:外展神经核长约1.16±0.17mm;外展神经副核长约0.80±0.06mm;面神经核长约0.96±0.07mm。外展神经核由中、小细胞构成,中型细胞占74.4％,小细胞占25.6％;外展神经副核由中、小细胞构成,中型细胞占26.8％,小细胞占73.2％;面神经核由大、中、小细胞构成,大细胞占15.6％,中型细胞占80.4％,小细胞占4.0％。结合禽类和哺乳类的有关文献,对北京鸭脑的上述核团的形态、位置和亚核划分与其它禽类和哺乳动物的相应核团进行了比较和讨论。     

低氧和高氧对大菱鲆幼鱼红细胞核异常及氧化抗氧化平衡的影响

研究了极低氧(0.2、1.0、2.0 mg/L)、低氧(3.0、5.0 mg/L)和高氧(11.0、14.0 mg/L)处理28 d期间,大菱鲆幼鱼外周血红细胞核异常以及血清超氧化物岐化酶(SOD)、过氧化氢酶(CAT)活力和肝脏丙二醛(MDA)含量等氧化抗氧化平衡中参数的变化。结果显示,极低氧条件下,0.2、1.0和2.0 mg/L组的实验鱼分别在处理2 h、1 d和2 d内全部死亡,其余各组则没有出现死亡现象。溶解氧(DO)对大菱鲆核异常包括微核及总核异常率的影响表现出明显的时间和剂量累积效应,到28 d时才在5.0、14.0 mg/L组和对照组中(正常充气,约7.0 mg/L)观察到微核现象。恢复正常充氧14 d后,高氧组和对照组中仍有微核现象出现。2 h时极低氧组SOD活力较高。其他各处理时间时,SOD活力在各组之间差异显著。各组CAT活力在2 h时未发生明显变化,在7、28和42 d时差异显著(P<0.05)。极低氧组大菱鲆肝脏MDA含量较低,低氧组和高氧组MDA含量均随着处理时间的延长逐渐降低。恢复后低氧组MDA含量显著升高(P<0.05)。研究还发现,对照组大菱鲆也会受到过氧化的胁迫作用,而5....     

重复超排技术在纯种和牛核心群建立中的应用

为了快速扩繁纯种和牛核心群,以最大限度发挥其繁殖潜力。试验以南北方3个典型地区的纯种和牛为试验对象,采用CIDR+FSH+PG方法进行超数排卵,探索合理的重复超排次数及此技术在不同地域环境下的应用状况。高强度重复超排试验表明：初次超排获得的可用胚胎数最理想,随着超排次数的增加,可用胚胎数量有所降低,但是差异不显著(P＞0.05);地域间不同超排间隔的超排数据比较,不同地区超排效果差异不显著(7.38±4.27)、(7.88±5.95)、(7.45±5.24)(P>0.05)。在良好的饲养管理前提下,高强度重复超排以1个月为超排间隔,超排3~4次为宜;纯种和牛的适应能力较强,超排间隔在1-2个月,超排3次的常规超排方案可以应用于南北方地区和牛核心群的建立。     

鲁西黄牛开放和闭锁核心群育种方案的对比分析

鲁西黄牛是中国优良的地方品种,为了更有效的制定适宜的育种方案,加快鲁西黄牛肉用品系的选育,本研究在鲁西黄牛青年公牛体系和核心群育种方案的基础上,分别设定开放和闭锁核心群育种方案,采用确定性模型评估了开放和闭锁核心群育种体系的育种效果。结果表明,开放与闭锁核心群育种方案相比,综合遗传进展提高了0.80％;育种产出量提高了9.39％;育种效益提高了10.64％;世代间隔延长了0.90％,开放核心群体系优于闭锁核心群体系。这充分说明,在鲁西牛核心群育种方案中,开放核心群育种体系优于闭锁核心群育种体系。     

40%丙环唑·多菌灵悬浮剂防治油菜菌核病应用试验

田间药效试验表明40%丙环唑.多菌灵悬浮剂对油菜菌核病有良好的防治效果,适宜用量70～90mL/667m2,防治效果达80%以上。     

香菇完全亲和双-单杂交及其先导核的验证

选择香菇的野生株 （ Ｑ） 与栽培株苏香两种孢子单核体 （ Ａ１ Ｂ１, Ａ２ Ｂ２） 进行完全亲和双单杂交, 以拮抗试验辅以液体出菇试验初步鉴定双单杂交后代, 并以 Ｒ Ａ Ｐ Ｄ技术 （随机扩增的多态性 Ｄ Ｎ Ａ 技术） 对杂交后代进行检测。每个杂交组合只得到一个杂交后代, 说明在完全亲和双单杂交供体中存在先导核。运用原生质体单核化与交配型鉴定技术对杂交后代中来自供体的先导核进行了追踪研究。结果表明, 受体交配型不同时, 其先导核也不同。在 （ Ａ３ Ｂ３＋ Ａ４ Ｂ４） × Ａ１ Ｂ１双单杂交中先导核为 Ａ３ Ｂ３ 核, 在 （ Ａ３ Ｂ３＋ Ａ４ Ｂ４） × Ａ２ Ｂ２ 双单杂交中先导核为 Ａ４ Ｂ４ 核。     

两用核不育系的育性稳定性研究

本试验利用人工气候厢和不同海拔高度研究了１１个两用核不育系不育性的稳定性，结果表明，Ｔ５Ｓ－４，２５５８－１Ｓ，７０７４３Ｓ和Ｍ９０１Ｓ对低温表现钝感现象，且良性转化乇底，可望在水稻育中利用。     

补阳还五汤联合BMSCs移植对脊髓损伤气虚血瘀证红核神经元的影响

目的 探讨补阳还五汤（Buyang Huanwu Decoction,BYHWD）联合骨髓间充质干细胞（bone marrow mesenchymal stem cells,BMSCs）移植对保护脊髓损伤（sipnal cord injury,SCI）气虚血瘀证红核神经元是否能产生协同增效作用。方法 将30只雄性SD大鼠随机分为5组：正常组、气虚血瘀组、BYHWD组、BMSCs组、BYHWD+BMSCs组,每组6只。采用游泳力竭法联合红核脊髓束（rubrospinal tract,RST）横断手术制备气虚血瘀证大鼠模型,并予以相应干预。通过斜板实验观察运动功能恢复情况,尼氏染色观察红核神经元胞体形态及数量,在RST横断点上方注射荧光金（FG）进行神经束路示踪,观察红核神经元胞体形态及数量。结果 术后14 d、28 d,BYHWD+BMSCs组斜板实验评分高于气虚血瘀组、BYHWD组及BMSCs组（P<0.05或P<0.01）。尼氏染色显示与正常组相比,气虚血瘀组尼氏体体积及数量减少,胞浆染色较浅;BYHWD组、BYHWD+BMSCs组的情况优于气虚血瘀组,尤其是BYHWD+BMSCs组。FG逆行示踪显示气虚血瘀组胞体荧光强度减弱、形态不规则、边缘模糊不清,且红核正常神经元数目较正常组明显减少（P<0.01）,BYHWD组、BMSCs组、BYHWD+BMSCs组较模型组增加（P<0.01）,特别是BYHWD+BMSCs组（P<0.01）。结论 BYHWD联合BMSCs移植对保护SCI气虚血瘀证逆行性溃变的红核神经元能产生协同增效作用。     

Ginsenoside Rg1 promotes growth and extracellular matrix synthesis in degenerative human lumbar nucleus pulposus cells by inhibiting Wnt/β-catenin pathway

AIM: To explore the role of ginsenoside Rg1 in the growth of degenerative human lumbar nucleus pulposus cells (HNPCs). METHODS: Cultured HNPCs were subjected to oxygen-glucose deprivation (OGD) to mimic the micro-environment of degenerative HNPCs. The morphological changes of the cells in control group and OGD group were observed under optical microscope. The cells were treated with ginsenoside Rg1 at concentrations of 25, 50 and 100 μmol/L. The expression of collagen Ⅱ and aggrecan at mRNA and protein levels was determined by real-time PCR and Western blot analysis. The cell viability was measured by CCK-8 assay. The mRNA level of Ki67 was detected by real-time PCR. The apoptosis was analyzed by flow cytometry. The activity of caspase-3 was measured by a caspase-3 kit. The expression of Wnt/β-catenin pathway-related proteins was determined by Western blot. Furthermore, the expression of Wnt/β-catenin pathway-related proteins, the cell viability and apoptosis, and the expression of extracellular matrix synthesis proteins were assessed after the cells were co-treated with LiCl and 100 μmol/L ginsenoside Rg1. RESULTS: Normal HNPCs attached on the cell culture plate faster, and were almost round with rich cytoplasm. However, the cell adherence was slower, and the cells were long fusiform with decreased cytoplasm after OGD treatment, indicating that the model of degenerative HNPCs was successfully established. Compared with normal HNPCs, the expression of collagen Ⅱ and aggrecan at mRNA and protein levels was decreased in OGD group (P<0.05), which was then increased after the cells were treated with ginsenoside Rg1 at 25, 50 and 100 μmol/L (P<0.05). Compared with normal HNPCs, the cell viability and Ki67 expression were decreased in OGD group (P<0.05), which were increased after treatment with ginsenoside Rg1 (P<0.05). Meanwhile, the apoptotic rate and caspase-3 activity were significantly increased in OGD-treated cells (P<0.05), which were decreased after treatment with ginsenoside Rg1 (P<0.05). In addition, the activation of Wnt/β-catenin pathway was also inhibited by ginsenoside Rg1 treatment at dose of 100 μmol/L (P<0.05). LiCl, a Wnt/β-catenin pathway agonist, obviously decreased the protective effects of ginenoside Rg1 on OGD-induced cells (P<0.05), indicating that the Wnt/β-catenin pathway was involved in the protective effects of ginenoside Rg1 on degenerative HNPCs. CONCLUSION: Ginsenoside Rg1 promotes growth and extracellular matrix synthesis of degenerative HNPCs through inhibiting Wnt/β-catenin pathway. This study will provide a new idea for prevention and treatment of degenerative HNPCs.