大鲵虹彩病毒流行株的分离及其主衣壳蛋白编码基因序列比较分析

大鲵虹彩病毒(giant salamander iridovirus, GSIV)是近年中国大陆新发现的引起人工养殖大鲵(Andrias davidianus)大规模死亡的病毒病原。为了揭示大鲵虹彩病毒流行株的基因型差异, 本研究对2010–2012年采集自全国不同大鲵养殖区域的患虹彩病毒病的大鲵样本进行了分子检测、病毒分离培养以及病毒主衣壳蛋白(major capsid protein, MCP)基因测序与比对分析。结果显示, 采自陕西、湖北、湖南、浙江、江西、福建等省的10个样本检测为阳性, 通过细胞培养获得10株病毒流行株。对该10株流行株MCP基因的测序与比对分析发现, 核苷酸序列相似性达到99.7%~100%, 其推测的氨基酸序列无明显差异, 证实中国大鲵虹彩病毒流行株属同一基因型。系统进化树分析结果表明, 所选大鲵虹彩病毒与蛙病毒分别聚为一枝, 但其亲缘关系较近。本研究结果旨为大鲵虹彩病毒病的疫苗研制及其免疫防控技术研究奠定基础。

     

我校非测绘专业测量学课程教学改革

对我校测量学教学改革进行研究与探讨,指出非测绘专业测量学教学中存在的问题,并有针对性地提出了测量学教学改革的对策。     

高职营销类专业多途径项目化教学的实践探索

高职院校营销类专业学生培养,应将多途径项目化教学与社会服务实践相融合,综合考虑目标、过程、应用、评估等方面,并与社会服务实践进行融合,最终达成社会服务能力建设、高职院校人才培养目标。     

Antigen-nonspecific macrophage factors modulating the antibody response in vitro

Antibody response to an antigen involves the co-operation between three types of cells: macrophages, T cells and B cells. The cognate interactions between these cells play a fundamental role in the expression of a specific antibody response, but the last is modulated by antigen-nonspecific soluble factors produced either by macrophages or by T cells. Macrophages elaborate a spectrum of molecules modulating the function of lymphoid cells; among them are IL1 and prostaglandins of the E series, which are respectively enhancer and inhibitor of the antibody response in vitro. These molecules alter T cell and B cell activities through different mechanisms involving activation or inhibition of IL2 production, or alteration of cells surface antigens. However, the cellular events following the fixation of soluble factor on its receptors are not known.     

关于落实南方集体林区重点产材县木材收购政策的思考

以浙江省２个重点产材县（市）遂昌县和临安市为基点，分析了南方集体林区重点产材县木材林收购政策难以落实到位的原因，认为主要是林业收益分配不合理，配套的林政管理措施没有跟上，木材公司经营机掉不完善。提出改革现行木材收购体制，调整林业税费，加强林政管理，转换木材公司经营机制等措施，使木材收购政策得以顺利实现和尽快落实到位。     

师范生免费教育政策下的英语专业学生英语学习动机类型

采用问卷调查并结合半结构式访谈，探讨在师范生免费教育政策的条件下英语专业学生的动机倾向性，尝试归纳出动机类型，旨在为师范生免费教育相关政策的推广以及高等师范免费教育的培养模式提供一定的借鉴。此次调查的受试学生为402名西南大学外国语学院2007年9月入学的首批英语专业免费师范生。用SPSS13．0对问卷调查后所得数据进行了因子分析，归纳出八种动机类型：个人发展动机，兴趣动机、社会责任动机、文化融合动机、家庭责任动机、政策动机、专业观念动机、证书动机。调查的结果反映出师范生免费教育政策给他们的学习动机带来了直接或间接的影响。     

数控椭圆专用铣床设计理论研究

以滑块椭圆机构原理设计数控椭圆专用铣床,该机构不需计算长短轴和焦距,用步进电机作为动力源可提高椭圆的加工精度,实现大批量自动化生产。     

基于职业岗位分析的课程体系开发探索——以环境监测与治理技术专业为例

针对职业教育理论与实践脱离的问题,以环境监测与治理技术专业为例,围绕该专业面向的职业岗位的要求,在职业岗位分析的基础上,构建出由＂职业基础课程＋职业岗位课程＋职业拓展课程＂组成的课程体系,突出了职业岗位要求在课程框架中的主线地位。     

Breeding for resistance to coffee berry disease in Coffea arabica L. II. Inheritance of the resistance

Summary The inheritance of resistance to coffee berry disease (CBD) has been studied by applying a preselection test to F₂ progenies of a half diallel cross between 11 coffee varieties with different degrees of resistance and to sets of parental, F₁, F₂, B₁₁ and B₁₂ generations of crosses between resistant and susceptible varieties. True resistance to CBD appears to be controlled by major genes on three different loci. The highly resistant variety Rume Sudan carries the dominant R- and the recessive K-genes. The non-allelic interaction between these two genes is of a duplicate nature. The R-locus has multiple alleles with R ₁R₁alleles present in Rume Sudan and the somewhat less effective R ₂R₂alleles in a variety like Pretoria, which also has the K-gene. The moderately resistant variety K7 carries only the recessive K-gene. The arabica-like variety Hibrido de Timor (a natural interspecific arabica x robusta hybrid) carries one gene for CBD resistance on the T-locus with intermediate gene action. It probably inherited this gene from its robusta parent. There is circumstantial evidence that the resistance to CBD is of a stable nature, but it is advisable to accumulate in one genotype as many resistance genes as possible by combining in the breeding programme the resistance of Rume Sudan with that of Hibrido de Timor.     

Major genes for resistance to beet necrotic yellow vein virus (BNYVV) in Beta vulgaris

Summary Inheritance of resistance to beet necrotic yellow vein virus (BNYVV) was studied in segregating F2 and backcross families obtained from crosses between resistant plants of the sugar beet selection Holly-1-4 or the wild beet accession Beta vulgaris subsp. maritima WB42 and susceptible parents. Greenhouse tests were carried out, in which seedlings were grown in a mixture of sand and infested soil. Virus concentrations of BNYVV in the rootlets were estimated by ELISA. To discriminate resistant and susceptible plants, mixtures of normal distributions were fitted to log₁₀ virus concentrations, estimated for segregating F1, F2 and BC populations of both accessions. The hypothesis that Holly-1-4 contained one single dominant major gene was accepted. For WB42, results fitted with the hypotheses that resistance was based on either one (or more) dominant major gene(s) showing distorted segregation, or two complementary dominant genes, which are both required for resistance. Resistance from WB42 appeared to be more effective against BNYVV than resistance from Holly-1-4.This research was carried out as part of a PhD study at the Graduate School Experimental Plant Sciences (EPS), Department of Virology, Wageningen, The Netherlands