Epidemiology of beet necrotic yellow vein virus in sugar beet at different initial inoculum levels in the presence or absence of irrigation

A field experiment was set up in 1988 to study the development of rhizomania disease of sugar beet at different inoculum levels of beet necrotic yellow vein virus (BNYVV) in soil. Five, tenfold different, inoculum levels were created by addition of the approximate amounts of 0, 0.5, 5, 50 and 500 kg infested soil per ha (the latter corresponding to 0.01% v/v calculated to the tillage layer). A drip irrigation treatment was applied to study the influence of soil moisture on disease. Susceptible sugar beet, cv. Regina, was grown for three consecutive years.In the first year, root symptoms were not observed, but BNYVV-infected plants were detected by ELISA in low numbers at all inoculum levels at harvest. After late drilling in 1989, high numbers of infected plants, up to 90–100% in plots with the highest inoculum level, were detected already in June. Root symptoms were also observed from June onwards. In both these years disease incidence increased in time and was significantly influenced by the initial inoculum level. In the third year, the whole field was heavily diseased, and only for the non-irrigated plots incidence differed for different initial inoculum levels. The expression of symptoms by BNYVV-infected plants was influenced by initial inoculum level, thus by the amount and timing of primary infection.Root weight at harvest was not affected, but sugar content decreased with increasing inoculum level already in 1988, leading to a reduction in sugar yield of 10% at the highest inoculum level. In 1989, both root weight and sugar content decreased progressively with increasing inoculum level, resulting in sugar yield reductions of 11–66% (down to approximately 3000 kg ha^–1) for low to high inoculum levels compared to the control. As the control plots became contaminated, all yields were low in 1990, still showing a decrease with increasing inoculum level in the non-irrigated plots, but an overall mean sugar yield of 3323 kg ha^–1 for the irrigated ones.Sodium and -amino nitrogen content of the root, additional quality parameters determining extractability of sucrose, showed an increase and decrease, respectively, with increasing initial inoculum level already in the first year. The relative differences in contents compared to those from the control were largest for Na content. A significant negative correlation was found between Na (mmol kg^–1 root) and sugar content (% of fresh weight); linear for 1988, exponential for 1989 and 1990.In spring 1989, the infestation of individual plots was assessed using a quantitative bioassay estimating most probable numbers (MPNs) of infective units of BNYVV per 100 g dry soil. The relationship between the MPns determined and root weight, sugar content and sugar yield at harvest could be described by Gompertz curves. The increase in disease incidence with increasing MPN in 1989 was adequately fitted with a logistic equation.     

Sensitivity,specificity, and predictive values of reagent test strip estimations of blood urea nitrogen

Azostix-reagent-tests(R) strips (Ames, Miles, Inc., Diagnostic Division, Elkhart, IN) were used to measure blood urea nitrogen values in blood samples from 125 dogs and cats at the North Carolina State University, College of Veterinary Medicine. Results of the tests were compared with standard serum urea nitrogen results. Sensitivity, specificity, and negative predictive values were high (86.4, 90.3, and 96.5%, respectively). Positive predictive value was low, 65.5% of the dogs and cats with elevated blood urea nitrogen values were correctly classified as abnormal The test performs well when the prevalence of abnormal values is near 50%.     

低氧胁迫下黄瓜幼苗根系多胺代谢的变化

 利用外源Spd、PAO 抑制剂研究了黄瓜幼苗根系在低氧水培时的生长状况以及内源PAs、H₂O₂ 含量和PAO活性的动态变化。结果表明, 外源PAs对低氧胁迫下黄瓜幼苗根系的生长有促进作用;低氧处理后3种PAs含量都明显上升, 外源Spd和PAO抑制剂增加了根系中的PAs含量, 降低了根系中H₂O₂ 含量, 减缓了低氧伤害; PAs为低氧胁迫的应激产物, PAO为适应酶类, 其活性随PAs含量的变化而发生变化。     

Effects of Acclimate™ on the frequency and duration of aggressive sequence and growth performance in co-mingled, weaned pigs

Co-mingling weaned pigs often results in aggressive behavior which can result in injury and reduced performance. This experiment examined the effect of Acclimate™, a product reported to reduce equine aggression, on weaned pig aggression and growth performance. Weanling pigs (n=214, avg. wt.=5.93 kg) were blocked by weight, sex and litter (n=25) and randomly assigned to either Acclimate™ treatment or a control group. Acclimate™ treated pigs were marked on the nose, face and neck. Pigs were individually identified and videotaped to determine frequency of aggressive sequence (FAS) and duration of aggression (DOA) on an individual pig basis. Pig weights and feed consumption were used to determine feed intake, total weight gain (TWG), average daily gain (ADG) and feed to gain ratio (F/G). Differences between litter, sex and treatment for FAS, DOA, F/G and ADG were tested using GLM, SAS. Neither Acclimate™ treatment or sex had a significant effect on FAS, TWG or F/G. However, there was a trend towards reduced duration and intensity of aggression in Acclimate™ treated pigs (p=0.1). There was a significant effect of litter (p<0.01) and TWG (p<0.001) on FAS at both 1 and 3 weeks. Acclimate™ treatment of weaned pigs does not decrease FAS, but may decrease the intensity and duration of aggression without negatively effecting growth.     

饲粮中添加抗菌肽对肉鸭增重及血清尿素氮、总蛋白水平的影响

240只1日龄肉鸭随机成分4组,每组设3个重复,对照组用基础日粮,试验组在基础日粮上每千克分别添加1、2、3mL抗菌肽制剂。结果显示:1)2、3mL/kg添加量组增重效果显著(P<0.05),3mL/kg添加量组最佳;净肉率升高,达到显著水平(P<0.05),其中主要是胸肌率上升;腹脂率降低但差异不显著(P>0.05);料重比无显著变化(P>0.05)。2)血清总蛋白浓度差异不显著(P>0.05),但尿素氮浓度下降,2、3mL/kg添加量组达显著水平(P<0.05),3mL/kg添加量组最低。     

水稻旱作对其生长量和经济产量的影响

1998～1999年，研究了水稻旱作条件下，不同生育时期受水分胁迫后对作物生长量的影响。结果表明：不同指标对水分胁迫时期的敏感性不一，植株高度对抽穗期水分胁迫最敏感；单株绿叶面积和单株地上部干重对幼穗分化后期水分胁迫最敏感；根系干重和根冠比对幼穗分化前期水分胁迫最敏感；幼穗分化后期水分胁迫对经济产量影响程度最大，无效分蘖期水分胁迫对产量影响程度最小。     

Variation in the development of secondary dormancy in oilseed rape genotypes under conditions of stress

Summary A substantial amount of seed is left in the fields before and during harvest of oilseed rape. Although this crop exhibits little or no primary dormancy, the absence of certain environmental cues that promote germination of imbibed seeds induces secondary dormancy. The work reported investigated the extent to which environmental stress conditions, including osmotic stress, low oxygen stress and anaerobiosis, induce secondary dormancy in oilseed rape, and examined the variation in development of secondary dormancy between and within genotypes. Osmotic stress was most effective in inducing dormancy. Anaerobic treatment produced very few dormant seeds, as did an atmosphere low in oxygen and high in nitrogen. The development of secondary dormancy under osmotic stress varied considerably between and within genotypes. Dormancy ranged from almost zero to about 60% for winter genotypes and about 85% for spring types. Within genotypes, variations occurred between seed lots and years of harvest. Temperature variations affected the percentage of dormant seeds. More dormant seeds were likely to be produced with incubation under water stress at 20 °C than at 12 °C. In winter genotypes, fewer dormant seeds were produced when incubation temperature and germination test temperatures differed. Thus, incubating at 20 °C and 12 °C, followed by germination tests at 20 °C and 12 °C, respectively, produced most dormant seeds. Also, in the winter genotypes, the potential development of secondary dormancy was positively correlated with the pattern and speed of germination of untreated seeds.     

碳酸氢根和铵态氮共同对菜豆生长及养分吸收的影响

 以菜豆为材料进行水培试验, 探讨了高浓度NH₄⁺-N与HCO₃^-共存介质对菜豆生长发育及其营养吸收的影响, 结果表明: ①以HCO₃^--N 为主要氮源时, 菜豆生长状况最好; 以NH₄⁺-N为主要氮源且无HCO₃^-存在时, 培养前期菜豆根系就发生受害现象, 叶片出现萎蔫; 但加入HCO₃^-后, 随着营养液中HCO3- 浓度从0 升高到13. 0 mmol/L , 菜豆长势逐渐改善, 表明HCO₃^-与NH4+ 以较合适的比例共存于介质中可明显减轻二者非共存对菜豆生长的抑制作用。② 同一处理, 菜豆整株对主要营养元素吸收量的次序为: N > K> P > Ca >Mg > Fe >Mn , 以NH4+2N 为主要氮源时, 随HCO₃^-浓度升高, 菜豆对N、P、Ca、Mn 的吸收量逐渐增大。③以NO_3^--N 为主要氮源, P、Fe、Mn 元素在根部出现累积, 而以NH₄⁺-N为主要氮源时, 随HCO₃^-浓度增加, N、P、Ca、Mn 在根部亦有累积现象。     

NaCl胁迫下枸杞愈伤组织活性氧产生与质膜H+—ATPase活性的关系

以枸杞愈伤组织为材料,研究了盐胁迫对活性氧伤害和质膜H -ATPase活性的影响。结果表明,NaCl浓度为100mmol/L时超氧阴离子(O2-·)和过氧化氢(H2O2)含量上升,质膜H -ATPase活性先升后降;质膜相对透性与丙二醛(MDA)含量呈显著正相关;超氧阴离子(O2-·)和过氧化氢(H2O2)含量与质膜H -ATPase活性在重度胁迫下呈显著负相关,说明盐胁迫下活性氧积累可能是加速伤害质膜功能的主要原因之一。     

盐胁迫对果树光合生理的影响

结合我们相关的研究结果综述了盐胁迫对果树光合作用的影响。总体来说盐胁迫导致果树净光合速率下降,且这种作用受树种、品种、砧木以及盐的种类、浓度和处理时期的影响;盐胁迫还使叶片干物质积累下降并影响碳水化合物的组成。盐逆境所产生的渗透胁迫和离子毒害而引起的气孔性限制和非气孔性限制则是果树叶片净光合速率下降的原因。此外,盐胁迫使果树叶片的叶绿素荧光效率下降。文章最后还提了出今后研究果树盐胁迫与光合作用的关系应与实际的生产环境相结合。