Effect of wilting,silage additive,PEG treatment and tannin content on the distribution of N between different fractions after ensiling of three different sainfoin (Onobrychis viciifolia) varieties

Sainfoin (Onobrychis viciifolia) is a tanniniferous, leguminous plant that has potentially beneficial effects on protein utilization in ruminants. As ensiling causes protein breakdown and elevated levels of buffer soluble N (BSN), we studied the distribution of N before and after ensiling sainfoin. Three varieties of sainfoin were either direct‐cut and frozen directly or wilted and frozen before later ensiling in mini‐silos with and without acidification with Promyr (PM; an acidifying commercial mixture of propionic and formic acid) and with or without polyethylene glycol (PEG). Extractable tannins (ET) and protein‐bound tannins (PBT) were measured with an HCl/butanol method in an attempt to correlate tannin levels to N fractions. The sainfoin silages showed good ensiling characteristics and had relatively high concentrations of undegraded protein. The effect of wilting on BSN levels (g/kg N) was dependent on sainfoin variety (P < 0·001). PEG increased and PM decreased the level of BSN in the silages (P < 0·001). PM treatment also produced less non‐protein N and ammonia‐N (P < 0·05) as compared with no additive. Addition of PEG to the silage increased the BSN‐proportion 1·8‐ and 2·6‐fold for both DM stages. A strong tannin‐protein binding effect is, therefore, confirmed in sainfoin. However, correlations between tannin levels (ET and PBT) and BSN were poor in the (non‐PEG) silages, indicating either that the HCl/butanol method is unsuitable for measuring tannin in silages or that qualitative attributes of tannins are more relevant than quantitative. The HCl/butanol method seems therefore not to be useful to predict degradation of protein in sainfoin silages.     

Nutrient‐Release Rates of Controlled‐Release Fertilizers in Forest Soil

Abstract

Nutrient‐release rates of controlled‐release fertilizer (CRF) with four different labeled release periods were evaluated. Samples (30 g) sealed with nylon mesh were buried at a clearcut forest site (Price soil series) in western Oregon, USA, in February 2000 and excavated every 7 weeks for 14 months to determine residual weight and composition. Cool, dry soil conditions apparently prolonged nutrient release beyond labeled rates; the fertilizer with the shortest release period (3–4 months) released approximately 72% of the fertilizer (by weight), whereas that with the longest release period (8–9 months) released 48%. Release varied among individual nutrients [nitrate (NO₃)>ammonia (NH₃)>potassium (K)>sulfur (S)>magnesium (Mg)>phosphorus (P)]. Minimal changes in micronutrient [iron (Fe), manganese (Mn), zinc (Zn), and molybdenum (Mo)] contents were attributed to the formation of insoluble compounds with P. Variable release among individual nutrients demonstrates a limitation toward delivering a full range of nutrients and suggests that further refinement of CRF technology is needed to optimize nutrient availability under realistic field conditions.     

Estimation of litchi (Litchi chinensis Sonn.) leaf nitrogen content at different growth stages using canopy reflectance spectra

The estimation of crop nitrogen status in fresh vegetation leaf using field spectroscopy is challenging due to the weak responses on leaf/canopy reflectance and the overlapping with the absorption features of other compounds. Although the spectral indices were proposed in the literature to predict leaf nitrogen content (LNC), the performance of selected spectral indices to estimate the LNC is often inconsistent. Moreover, the models for nitrogen content estimation changed with the growth stage. The goal of this study was to evaluate the performance of published indices, ratio of data difference index (RDDI) and ratio of data index (RDI) developed by band iterative-optimization algorithm in LNC estimation. The correlation analysis, linear regression and cross validation were used to analyze the relationship between spectral data and LNC and construct the best performed estimation model. The study was conducted by the data of five growing seasons of litchi from the orchards in Guangdong Province of China. Results showed that the relationship between chlorophyll (Chl) related spectral indices and LNC varied with the growth stage. Even in flower bud morphological differentiation stage and autumn shoot maturation stage, there were not significant correlations between the proposed spectral indices and LNC. Besides it is difficult to estimate the LNC by the general model across the growth stages due to the integrated effects of cultivar, biochemical, canopy structure, etc. The band iterative-optimization algorithm can improve the sensitivity of spectral data to LNC to some extent. The optimal RDDI performed better than other indices for the synthetic dataset and the dataset in each growth stage. And the sensitive bands selected in the optimal indices at each growth stage are not consistent, which are not only related to the Chl absorption but also other biochemical components, such as starch, lignin, cellulose, protein, etc. In general, the LNC can be estimated by the optimized CR-based RDDI indices in autumn shoot maturation stage, flower spike stage, fruit maturation stage, and flowering stage with the R² > 0.50 and RMSE < 0.14. Although there were the significant relationship between RDIs and RDDIs in flower bud morphological differentiation stage, the highest R²s of the model developed by RDDIs and RDIs were less than 0.50 in cross validation. This study indicated that the applicability of canopy reflectance to estimate litchi LNC was closely related to the growth stage of litchi. Growth stage-specific models will be preferred for estimating litchi LNC estimation.     

油菜新品种宁杂1818氮肥运筹试验

以油菜(Brassica campestris L.)新品种宁杂1818为试验材料,以秦优7号为对照,研究不同氮肥运筹条件下,宁杂1818的植株长势和产量变化,旨在为深入了解宁杂1818的生长特性,建立宁杂1818高产、高效栽培技术体系提供理论依据。     

Do combined applications of crop residues and inorganic fertilizer lower emission of N2O from soil?

Emissions of N₂O were measured following addition of ¹⁵N‐labelled residues of tropical plant species [Vigna unguiculata (cowpea), Mucuna pruriens and Leucaena leucocephala] to a Ferric Luvisol from Ghana at a rate of 100 mg N/kg soil under controlled environment conditions. Residues were also applied in different ratio combinations with inorganic N fertilizer, at a total rate of 100 mg N/kg soil. N₂O emissions were increased after addition of residues, and further increased with combined (ratio) applications of residues and inorganic N fertilizer. However, ¹⁵N‐N₂O production was low and short‐lived in all treatments, suggesting that most of the measured N₂O‐N was derived from the applied fertilizer or native soil mineral N pools. There was no consistent trend in magnitude of emissions with increasing proportion of inorganic fertilizer in the application. The positive interactive effect between residue‐ and fertilizer‐N sources was most pronounced in the 25:75 Leucaena:fertilizer and cowpea:fertilizer treatments where 1082 and 1130 mg N₂O‐N/g residue were emitted over 30 days. N₂O (log_e) emission from all residue amended treatments was positively correlated with the residue C:N ratio, and negatively correlated with residue polyphenol content, polyphenol:N ratio and (lignin + polyphenol):N ratio, indicating the role of residue chemical composition in regulating emissions even when combined with inorganic fertilizer. The positive interactive effect in our treatments suggests that it is unlikely that combined applications of residues and inorganic fertilizer can lower N₂O emissions unless the residue is of very low quality promoting strong immobilisation of soil mineral N.     

有机无机生物活性肥料施用技术对蔬菜作物生长及产量的影响

田间试验结果表明:施用有机无机生物活性肥料能明显提高蔬菜作物长势,提高甘蓝抗根腐和烂心病能力。在等氮条件下,与常规对照相比产量基本持平,差异并不显著;但每666.7m2可节约成本24元左右,幅度达30,7%。椐统计分析,甘蓝最佳施肥置以100kg/666,7m2为宜,此用量可比常规对照增产9.3%,每666.7m2增加收入295元。在施用总量不变的条件下,减少有机无机生物活性肥料基肥比例,甘蓝则有碱产趋势。     

Dietary fiber in a low-protein diet during gestation affects nitrogen excretion in primiparous gilts,with possible influences from the gut microbiota

We investigated the effects of dietary fiber (DF) supplementation in normal or low crude protein (CP) diets on reproductive performance and nitrogen (N) utilization in primiparous gilts. In total, 77 Landrace × Yorkshire pregnant gilts were randomly allocated to four dietary treatments in a 2 × 2 factorial design. The groups comprised 1) equal intake of normal CP (12.82% and 0.61% total lysine), 2) low CP (LP) (10.53% and 0.61% total lysine), and 3) with or 4) without DF supplementation (cellulose, inulin, and pectin in a 34:10:1 ratio). A low-protein diet during gestation significantly reduced daily weight gain from days 91 to 110 of pregnancy (−162.5 g/d, P = 0.004). From N balance trials conducted at days 35 to 38, 65 to 68, and 95 to 98 of pregnancy, DF addition increased fecal N excretion at days 65 to 68 (+24.1%) and 95 to 98 (+13.8%) of pregnancy (P < 0.05) but reduced urinary N excretion (P < 0.05), resulting in greater N retention at each gestational stage. DF increased fecal microbial protein levels and excretion during gestation. An LP diet also reduced urinary N excretion at different gestational stages. An in vitro fermentation trial on culture media with nonprotein N urea and ammonium bicarbonate (NH₄HCO₃) as the only N sources revealed that microbiota derived from feces of gestating gilts fed the high DF diet exhibited a greater capacity to convert nonprotein N to microbial protein. Microbial fecal diversity, as measured by 16S rRNA sequencing, revealed significant changes from DF but not CP diets. Gilts fed an LP diet had a higher number of stillbirths (+0.83 per litter, P = 0.046) and a lower piglet birth weight (1.52 vs. 1.37 kg, P = 0.006), regardless of DF levels. Collectively, DF supplementation to gestation diets shifted N excretion from urine to feces in the form of microbial protein, suggesting that the microbiota had a putative role in controlling N utilization from DF. Additionally, a low-protein diet during gestation negatively affected the litter performance of gilts.     

Selenium supplementation improves nutrient intake and digestibility,and mitigates CH4 emissions from sheep grazed on the mixed pasture of alfalfa and tall fescue

Low selenium (Se) in soil and forage can adversely affect on the quality of animal-derived foods, and hence on human health. Lambs grazed on mixed pastures of alfalfa (Medicago sativa) and tall fescue (Festuca arundinacea) were supplemented with five levels of Se [0, 3, 6, 9 and 12 µg/kg body weight (BW)]. The intake of dry matter (DM) and organic matter (OM) varied with the level of Se supplementation, with a peak at 6 µg Se per kg BW (p ≤ 0.05). Gross energy (GE) intake, digestive energy (DE) intake and metabolic energy (ME) intake were higher at 6 µg Se per kg BW than at other Se levels (p < 0.01); in addition, methane energy (CH₄-E) output was lower at 6 µg Se per kg BW. Supplementation with Se significantly increased nitrogen (N) intake, faecal N and urine N, for which the peak values were 20.2 g N/, 5.62 g N/day and 7.92 g N/day, respectively, at 6 µg Se per kg BW. Se intake, blood Se, faecal Se, urine Se and retained Se were negatively correlated with forage crude protein (CP) content (p < 0.001) but were positively correlated with the content of neutral detergent fibre (NDF) (p < 0.001) and acid detergent fibre (ADF) (p < 0.001). Thus, we recommend the addition of 6 µg Se per kg BW to sheep grazed on pastures in regions with low soil Se.     

0~4周龄云岭红肉鸡赖氨酸适宜需要量的研究

试验通过分析饲粮不同赖氨酸水平对云岭红肉鸡生长性能、氮沉积率及血液生化指标的影响,确定云岭红肉鸡饲粮赖氨酸的适宜需要量。采用单因素试验设计,将180只健康状况良好、体重相近的1日龄云岭红肉鸡随机分为5个处理组,每组3个重复,每个重复12只鸡。5组试验鸡饲喂的饲粮赖氨酸水平分别为0.85%、0.95%、1.05%、1.15%、1.25%。结果表明:赖氨酸水平对试验鸡的期末体重、全期增重、平均日增重及料重比有显著影响(P<0.05),当赖氨酸水平为1.15%时期末体重最大739.21 g,全期增重最大701.66 g,平均日增重最大24.19 g,料重比最低1.68。不同赖氨酸水平对丝氨酸、甘氨酸和组氨酸的表观代谢率影响显著(P<0.05)。对血清总氨基酸和血清尿素氮影响显著(P<0.05)。研究表明,0~4周龄云岭红肉鸡适宜赖氨酸水平最适量为1.15%。     

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《家禽屠宰检疫规程》在规范家禽的屠宰检疫,加强家禽产品的检疫活动管理,保障动物源食品安全方面发挥了重要作用。本文概述了《家禽屠宰检疫规程》关于家禽屠宰场所定义、规程适用动物、检疫对象、检疫合格标准的判定等方面存在的问题,特别是针对家禽产品入库贮藏后才出厂如何出具检疫合格证明,提出可采取贮藏后继续调运或分销的检疫方式出具检疫证明这一解决方案。这提示在今后工作中扩大屠宰检疫的适用范围及检疫对象,进一步修订和完善屠宰检疫规程,提升屠宰检疫技术水平。