Long-term impact of fertilization on activity and composition of bacterial communities and metabolic guilds in agricultural soil

To explore long-term impact of organic and inorganic fertilizers on microbial communities, we targeted both the total bacterial community and the autotrophic ammonia oxidizing bacteria (AOB) in soil from six treatments at an experimental field site established in 1956: cattle manure, sewage sludge, Ca(NO₃)₂, (NH₄)₂SO₄, unfertilized and unfertilized without crops. All plots, except the bare fallows, were cropped with maize. Effects on activity were assessed by measuring the basal respiration and substrate induced respiration (SIR) rates, and the potential activity of the AOB. To determine the bacterial community composition, 16S rRNA genes were used to fingerprint total soil communities by terminal restriction fragment length polymorphism analysis and AOB communities by denaturing gradient gel electrophoresis. The fertilization regimes had clear effects on both activity and composition of the soil communities. Basal respiration and r, which was kinetically derived as the exponentially growing fraction of the SIR-response, correlated well with the soil organic C content (r=0.93 and 0.66, respectively). Soil pH ranged from 3.97 to 6.26 in the treatments and was found to be an important factor influencing all microbial activities. pH correlated negatively with the ratio between basal respiration and SIR (r=0.90), indicating a decreased efficiency of heterotrophic microorganisms to convert organic carbon into microbial biomass in the most acid soils with pH 3.97 and 4.68 ((NH₄)₂SO₄ and sewage sludge fertilized plots, respectively). The lowest SIR and ammonia oxidation rates were also found in these treatments. In addition, these treatments exhibited individually different community fingerprints, showing that pH affected the composition of AOB and total bacterial communities. The manure fertilized plots harbored the most diverse AOB community and the pattern was linked to a high potential ammonia oxidation activity. Thus, the AOB community composition appeared to be more strongly linked to the activity than the total bacterial communities were, likely explained by physiological differences in the populations present.     

炭化生活污水污泥对酸化红壤的改良效果

从南京市、马鞍山市和丹阳市的3个污水处理厂采集污泥,在500℃下热解制备炭化污泥,测定了污泥和炭化污泥的性质和重金属含量,研究了污泥和炭化污泥对酸性红壤改良效果,并探讨了炭化污泥中重金属的环境风险。结果表明,污泥和炭化污泥中含有一定量的碱,添加炭化污泥可提高红壤的p H和交换性钙、镁和钾含量,降低土壤交换性铝和交换性H+含量。但污泥中大量铵态氮的硝化作用释放质子,抵消了污泥对红壤酸度的改良作用。添加污泥和炭化污泥提高了土壤有机碳、有效磷和速效钾的含量,提高了土壤肥力。污泥炭化后重金属含量有所增加,但大部分重金属的有效态含量下降,说明热解过程可以降低部分有毒重金属的活性。与对照相比,添加炭化污泥会增加土壤中部分重金属有效态含量,特别是有效态锌含量显著增加,因此炭化污泥农业利用存在一定的环境风险。建议将炭化污泥用于酸化的森林红壤的改良。     

N2O emissions from different cropping systems and from aerated,nitrifying and denitrifying tanks of a municipal waste water treatment plant

Nitrous oxide emissions, nitrate, water-soluble carbon and biological O₂ demand (BOD₅) were quantified in different cropping systems fertilized with varying amounts of nitrogen (clayey loam, October 1991 to May 1992), in an aerated tank (March 1993 to March 1994), and in the nitrification-denitrification unit (March to July 1994) of a municipal waste water treatment plant. In addition, the N₂O present in the soil body at different depths was determined (February to July 1994). N₂O was emitted by all cropping systems (mean releases 0.13–0.35 mg N₂O m^-2 h^-1), and all the units of the domestic waste water treatment plant (aerated tank 0–6.2 mg N₂O m^-2 h^-1, nitrification tank 0–204,3 mg N₂O m^-2, h^-1, denitrifying unit 0–2.2 mg N₂O m^-2 h^-1). During the N₂O-sampling periods estimated amounts of 0.9, 1.5, 2.4 and 1.4 kg N₂O–N ha^-1, respectively, were released by the cropping systems. The aerated, nitrifying and denitrifying tanks of the municipal waste water treatment plant released mean amounts of 9.1, 71.6 and 1.8 g N₂O–N m^-2, respectively, during the sampling periods.The N₂O emission were significantly positively correlated with nitrate concentrations in the field plots which received no N fertilizer and with the nitrogen content of the aerated sludge tank that received almost exclusively N in the form of NH ₄ ⁺ . Available carbon, in contrast, was significantly negatively correlated with the N₂O emitted in the soil fertilized with 80 kg N ha^-1 year. The significant negative correlation between the emitted N₂O and the carbon to nitrate ratio indicates that the lower the carbon to nitrate ratio the higher the amount of N₂O released. Increasing N₂O emissions seem to occur at electron donorto-acceptor ratios (C_H2_O or BOD₅-to-nitrate ratios) below 50 in the cropping systems and below 1200–1400 in the waste water treatment plant. The trapped N₂O in the soil body down to a depth of 90 cm demonstrates that agricultural production systems seem to contain a considerable pool of N₂O which may be reduced to N₂ on its way to the atmosphere, which may be transported to other environments or which may be released at sometime in the future.Dedicated to Professor J.C.G. Ottow on the occasion of his 60th birthday     

Effects of sewage sludge amendment on humic acids and microbiological properties of a semiarid Mediterranean soil

The residual effects of adding 40 t ha^–1 sewage sludge (SL) to a degraded soil cropped with barley were investigated after 9 and 36 months in a field experiment under semiarid conditions. The principal soil properties were apparently still affected by SL amendment 9 months after application but the effects disappeared after 36 months. With respect to control soil humic acids (HAs), the SL-HA was characterized by higher contents of S- and N-containing groups, smaller contents of acidic groups, a prevalent aliphaticity, extended molecular heterogeneity, and smaller degrees of aromatic polycondensation and humification. Amendment with SL caused an increase in N, H, S and aliphaticity contents and a decrease in C/N ratios and O and acidic functional group contents in soil HAs isolated 9 months after SL application. These effects tended to decrease after 36 months, most probably because the slightly humified SL-HA was mineralised over time through extended microbial oxidation, while only the most recalcitrant components such as S-containing and aromatic structures were partially accumulated by incorporation into soil HA. Microbial biomass, basal respiration, metabolic quotient and enzymatic activities increased in soil 9 months after SL application, possibly because of increased soil microbial metabolism and enhanced mineralisation processes. After 36 months these properties returned to values similar to those of the unamended soil, presumably due to the loss of energy sources.     

城市污水处理厂污泥处理处置及利用浅析

污泥的处理处置与利用是当今面临的重要问题.文章分析了我国污泥处理的现状,介绍了污泥的组成、性质,讨论了污泥处理处置的各种方法及利用方式,最终得出了污泥的材料化利用是污泥处置的理想途径的结论.     

UASB处理养猪场废水条件下进水浓度对污泥颗粒化的影响

UASB能够在很高负荷下稳定运行的重要原因在于反应器内形成的厌氧颗粒污泥。该文研究了利用UASB处理猪场废水时进水COD浓度对污泥颗粒化的影响，结果发现：颗粒直径随进水浓度的上升而增高，受传质过程中所能进入颗粒内部的营养量所控制，因此要根据COD去除率的情况适时提高进水浓度，及时地随微生物数量的增加补充营养，促进污泥颗粒化；也可以避免因浓度变化太快引起细菌生长过快，污泥结构松散，沉降性能下降，COD去除率和产气率降低。     

Initial results from long-term field studies at three sites on the effects of heavy metal-amended liquid sludges on soil microbial activity

In a long‐term study of the effects on soil fertility and microbial activity of heavy metals contained in sewage sludges, metal‐amended liquid sludges each with elevated Zn, Cu or Cd concentrations were applied over a 3‐year period (1995–1997) to three sites in England. The experiments were sited adjacent to experimental plots receiving metal‐rich sludge cakes enabling comparisons to be made between the effects of heavy metal additions in metal‐amended liquid sludges and sludge cakes. The liquid sludge additions were regarded as ‘worst case’ treatments in terms of likely metal availability, akin to a long‐term situation following sewage sludge additions where organic matter levels had declined and stabilised. The aim was to establish individual Zn (50–425 mg kg^?1), Cu (15–195 mg kg^?1) and Cd (0.3–4.0 mg kg^?1) metal dose–response treatments at each site, but with significantly smaller levels of organic matter addition than the corresponding sludge cake experiments. There were no differences (P > 0.05) in soil respiration rates, biomass carbon concentrations or most probable numbers of clover Rhizobium between the treatments at any of the sites at the end of the liquid sludge application programme. Soil heavy metal extractability differed between the metal‐amended liquid sludge and metal‐rich sludge cake treatments; Zn and Cd extractabilities were higher from the liquid sludge additions, whereas Cu extractability was higher from the sludge cake application. These differences in metal extractability in the treated soil samples reflected the contrasting NH₄NO₃ extractable metal contents of the metal‐amended liquid sludges and sludge cakes that were originally applied.     

塞内加尔达喀尔地区沙丘土壤中有机添加物的荧光表征及去向

The application of organic amendments on soils poor in organic matter (OM) can improve long-term soil fertility, but may also enhance the mineralization of native soil organic matter. Three organic amendments, compost, sewage sludge and horse manure used by urban market gardeners in Dakar, Senegal were analyzed for their OM maturity. Their fate was evaluated in a 45-d agronomic trial in a sandy Arenosol with lettuce. In each case, water-extractable organic matter (WEOM) and humic-like substances (HLS) were isolated from raw amendments and amended soils, and characterized using ultraviolet-visible (UV/Vis) spectroscopy. Results highlighted the general more aromatic character of HLS and WEOM fractions extracted from compost compared to the other two amendments. When applied to soils, however, these differences were not clearly observed. The aromaticity and humification degree of the labile fraction (WEOM) increased with depth in the first 30 cm for all amendments. This indicated the high lixiviation rates that fresh OM underwent in the studied sandy soil. Finally, a statistical analysis of the results was able to discriminate between surface and deeper horizons and between amended- and non-amended soil samples. Spectroscopic indices showed indeed strong increase/decrease with depth linked with the mineralization/humification processes that the fresh OM from amendments underwent during the 45 d of the agronomic trial. This study highlights the potential of spectroscopic techniques to study agricultural amendment organic matter fractions and their fate in soils.     

Cumulative Effect of Organic and Inorganic Sources of Nutrients on Yield,Nutrients Uptake and Economics by Rice–Wheat Cropping System in Indo-Gangetic Plains of India

ABSTRACT

Field experiments were conducted for 2 years in sandy loam soil, to study the direct effect of organic manures i.e. sewage sludge (SS), vermicompost (VC) and sesbania (SB) and chemical fertilizers on rice (Oryza sativa) and their residual effect on wheat (Triticum aestivum) grown in sequence in winter (Rabi) and summer (Kharif) season during 2015–2016 to 2016–2017 at Varanasi, Uttar Pradesh. Residual effect of organic sources of nutrients as SS, VC and SB were monitored up to fourth crop (II wheat) in sequence applied in conjunction with 75% recommended dose of fertilize (RDF). Among the nutrient sources, the maximum grain yield in I rice (4.89 t ha^?1), II rice (4.95 t ha^?1), was recorded in treatment T₃ (100% RDF with S, Zn, B) whereas in I wheat (4.68 t ha^?1) and II wheat (4.59 t ha^?1), it was recorded in T₄ (customized fertilizer). The maximum straw yield during all four crops was recorded in T₃ (100% RDF with S, Zn, B) in rice and T₄ (customized fertilizer) in wheat crop, which showed 25, 32, 23 and 28% increase over 100% RDF (T₂). Application of 100% RDF along with S, Zn, B and customized fertilizer increased the total uptake of N, P, K, S and B and also in net returns and B:C ratio followed by organic treatments.