In vitro binding of germanium to proteins of rice shoots

The possibility of in vitro binding between proteins of rice shoots and germanium (Ge) was investigated. The proteins in mixtures of aqueous extracts of rice shoots and radioactive germanium (⁶⁸GeO₂) were fractionated. The binding of radioactivity to the proteins was observed even after 5 successive fractionation steps from the original mixtures. At the final fractionation step using polyacrylamide gel electrophoresis, a constant proportionality between protein concentration and associated radioactivity was found in most samples although not all. These results indicate that the binding of ⁸⁸Ge to proteins is not due to the simple adsorption by proteins.     

A new method for measuring microbial biomass nitrogen in soils: Direct extraction after toluene treatment

Abstract

Phenolic compounds occurring naturally are reducing agents which react with hydrous Fe oxide and Mn oxides (Lehmann et al. 1987). Diphenols such as hydroquinone, resorcinol, and catechol were oxidatively darkened by the presence of hydrous Fe oxide though the degree of darkening was much less pronounced than that by Mn oxides (Shindo and Huang 1984). Okazaki et al. (1976) suggested that polyphenols are one of the materials responsible for the dissolution of Mn in a paddy soil under reducing conditions.     

Asymmetric warming effects on N dynamics and productivity in rice (Oryza sativa L.)

Climate warming exhibits strong diurnal variations, with higher warming rates being observed at nighttime, which significantly affects rice (Oryza sativa L.) growth and grain yield. The objective of this study was to determine the effects of asymmetric warming (all-day warming, AW; daytime warming from 07:00 to 19:00, DW; nighttime warming from 19:00 to 07:00, NW, and a control, CK) on rice nitrogen (N) dynamics and productivity. Two rice bucket warming experiments were performed in Nanjing in Jiangsu Province, China, using the free air temperature increase (FATI) technique. The daily mean temperatures in the rice canopy in the AW, DW and NW plots were 2.0, 1.1 and 1.3ºC higher than those in the rice canopy in the CK plots, respectively. The results indicated that the total N accumulation of rice was 8.27–40.53% higher in the warming treatment than in the control during the jointing, anthesis and maturity stages. However, there was no significant difference detected among the three warming treatments. The warming treatment substantially decreased N translocation efficiency, leading to the retention of more N in the plant stems during grain filling. The warming treatment also decreased the N harvest index, N utilization efficiency based on grain yield and N utilization efficiencies based on biomass in both growing seasons. The warming treatment significantly increased the aboveground biomass (9.26–16.18%) in the jointing stage but decreased it (2.75–9.63%) in the maturity stage. Although DW increased the carbon (C) gain by photosynthesis and NW increased the C loss by night respiration, the daytime higher-temperature treatment affected rice photosynthesis and reduced its photosynthetic rate and product. This effect may be one of the primary reasons for the insignificant difference in the aboveground biomass between the DW and NW treatments. In the AW, DW and NW plots, the grain yield was reduced by an average of 10.07, 5.05 and 7.89%, respectively, across both years. The effective panicles and grains per spike tended to decrease in the warmed plots, whereas irregular changes in the 1000-grain weight were observed. Our results suggest that under the anticipated climate warming, rice productivity would further decline in the Yangtze River Basin.     

灌溉方法对保护地土壤有机氮组分及剖面分布的影响

用Bremner有机氮分组法测定了连续7年采用不同灌溉方法灌溉的保护地土壤各剖面层次有机氮组分含量。结果表明,土壤全氮及有机氮各组分含量均随土层深度的增加而降低,但有机氮各组分占全氮的比例随土层深度增加的变化却无明显规律。用3种灌溉方法灌溉,不同土层间土壤有机氮各组分含量的差异主要存在于0～50 cm土层,50 cm以下差异很小;相同土层,土壤有机态氮含量均以酸解氮为主,且酸解氮中各组分绝对含量和相对含量的大小排列顺序均为,未知态氮〉氨态氮〉氨基酸态氮〉氨基糖态氮。在0～80 cm土层,土壤酸解氮占全氮的比例大多在58%～60%之间,只有渗灌处理0～10 cm,10～20 cm及沟灌处理0～10 cm土层酸解氮占全氮的比例较低,分别为34.21%,50.75%和48.02%;而非酸解氮占全氮的比例大多在32%～36%之间。3种灌溉方法相比较,除个别层次外,酸解氮中氨基酸态氮、氨基糖态氮及氨态氮占全氮的比例在各土层中滴灌和渗灌处理均高于沟灌处理,而酸解未知态氮和非酸解氮占全氮的比例则为沟灌处理高于滴灌和渗灌处理。     

传统稻鱼系统生产力提升对稻田水体环境的影响

如何在提升传统农业系统生产力的同时又不破坏其内涵、还对环境不产生负面影响,是一个受到研究者持续性关注的挑战性课题。本研究以具有1 200年历史的全球重要农业文化遗产稻鱼系统为例,研究稻鱼系统鱼的放养密度对生产力和环境的影响,探讨能够显著提升稻鱼系统生产力且又不破坏其传统性(产品优质、环境友好)的途径。研究表明,传统稻鱼系统(RF)(田鱼目标产量375 kg.hm 2)与水稻单作系统(RM)水体的化学需氧量(COD)、总氮(TN)、总磷(TP)及铵态氮(NH4+-N)含量均没有显著性差异。随着田鱼养殖密度提高(鱼产量由750 kg.hm 2提高到3 000 kg.hm 2)和饲料投入的相应增加,稻鱼系统的生产力和经济产出大幅度提高(稻鱼系统的净经济收入增加25.2%~101.4%),但稻鱼系统(RF)水体的COD值及TN、TP和NH4+-N含量均呈现增加的趋势,尤其是当鱼目标产量增至3 000 kg.hm 2时,水体TP含量和COD值显著提高,面源污染风险增加。分析还表明,鱼放养目标产量为2 250 kg.hm 2时,稻鱼系统经济效益最佳,此时净经济收入比传统稻鱼系统增加55.9%,并且不会对水体环境质量产生负面影响。     

利用航空成像光谱数据研究土壤供氮量及变量施肥对冬小麦长势影响

以推扫式成像光谱仪PHI(Pushbroom Hyperspectral Imager)获取的冬小麦拔节期、灌浆初期及乳熟期的航空影像数据为基础，提取反映冬小麦长势的光谱特征值，结合地面调查数据，分析了研究区冬小麦的长势情况；对不同时相光谱特征值与土壤基础供氮量、土壤总供氮量以及变量施肥量进行统计分析，分析结果显示：土壤基础供氮量、土壤总供氮量的空间分布差异对冬小麦长势有明显的影响，其中，土壤基础供氮量是影响冬小麦长势的重要因素，它对冬小麦的长势影响贯穿冬小麦的整个生育期；此外，该文还通过变量施肥前后作物     

Legume cover cropping effects on early growth and soil nitrogen supply in eucalypt plantations in south-western India

Growth and soil N supply in young Eucalyptus tereticornis stands at two sites in Kerala, India, were examined in response to cover cropping with three legume species (Pueraria phaseoloides, Stylosanthes hamata, and Mucuna bracteata). The effects of legume residues on soil N supply were investigated in a long-term (392 day) laboratory incubation using leaching micro-lysimeters. Residues from the eucalypt and legume species had different rates of net N release during the laboratory incubation. Net N release was significantly related to residue N concentration (R² =0.94), the C:N ratio (R² =0.91), the lignin:N ratio (R² =0.83), and the (lignin + soluble polyphenol):N ratio (R² =0.95). Nitrogen release rates declined in the order Mucuna > Pueraria > Eucalyptus > Stylosanthes. There was no net N release from Stylosanthes residues during the 392-day laboratory incubation, whereas Mucuna and Pueraria released N throughout the incubation period. Net N release from mixtures of legume and eucalypt residues was not additive in the early phase of the incubation, probably because eucalypt residues initially immobilized a portion of the legume-derived N in addition to the soil-derived N. Legume establishment had no significant effect on tree growth at one site (Kayampoovam), but resulted in depressed tree growth at the lower rainfall site (Punnala) at 18 months. There were no significant treatment effects on growth at Punnala after that time. Cover cropping with legumes during the early phase of forest plantation growth may be a useful mechanism to enhance soil N supply and optimize the synchrony between N supply and tree N uptake. Although these effects did not translate into improved plantation growth in the 3 years of this study, improved soil organic matter and N fertility may help ensure sustainable productivity over several rotations in the future. This study showed that the effect of legumes on N dynamics varies markedly with legume species. This, together with other factors (e.g. competition with trees, N fixation capacity), will be important in selecting suitable species for cover cropping in forest plantations.     

我国农田氮肥施用现状、问题及趋势

氮素在作物产量和品质形成中起着关键作用。本文综述了什么是合理施氮,包括施氮量、施氮方法和时期,也包括与有机肥和秸秆还田措施的配合等。指出我国农田氮肥施用的主要问题是施肥过程和施肥后的严重损失。依据农户调查所获得的田块尺度施氮量,与田间试验合理施氮量对比分析表明,过量施氮田块占总调查田块的大约33%。依据区域尺度单位播种面积平均施氮量,与作物平均推荐施氮量对比分析表明,全国过量施氮面积占播种面积20%、合理面积占70%、不足面积占10%。总体而言,过量施氮现象还相当普遍,特别是在蔬菜和果树等经济作物上。本文提出了一种估算国家尺度氮肥需求量的方法,可估算出全国合理需氮量范围,称之为氮肥需求量估算法。用三种不同方法估算的我国1980~2010年间的氮肥需求量与实际氮肥使用量比较表明,如仍然依照现在的粗放施肥习惯,应该为现在的实际氮肥使用量,5年平均为N 27.9×106t左右,正好处于合理需氮量范围的中线。在改善施肥技术基础上,我国2006~2010年间5年氮肥平均使用量应该在N 19.6×106t左右;用五种方法预测的我国未来氮肥需求量表明,如果改善施肥技术,我国2020、2030、2050年合理氮肥需求量分别为N 21.0×106t、21.7×106t、23.1×106t;如施肥技术得不到实质性改善,依然粗放施氮,则氮肥需求量应处于合理使用量范围的中线,分别为N 30.4×106t、31.4×106t、33.4×106t。进一步分析了我国粮食产量和氮肥施用量与美国和西欧的差异,我国农田有机肥和碳投入对增加土壤有机碳氮库的重要性。     

新型分层生物滴滤池去除污水中氮磷的性能研究

生物滴滤池是农村生活污水处理的主要技术之一,但其存在氮、磷去除能力有限,稳定性不高等缺点。为提高新型分层生物滴滤池的氮磷去除效率,探索最佳工艺条件,本文采用新型分层生物滴滤池为试验装置,考察了滤料种类、水力负荷、回流比等对装置去除污水中氮磷性能的影响。结果表明,当滤料为炉渣、水力负荷为4 m3·m-2·d-1、回流比为2∶1时滤池去除氮磷的效果最好,对NH4+-N、TN、TP、COD的平均去除率分别可达到87.08%、57.37%、66.04%、80.78%;采用较高的回流比是滴滤池提高脱氮效果的一条有效途径。