基于分组注意力模块的实时农作物病害叶片语义分割模型

针对传统农作物病害识别方法准确率低、鲁棒性差等问题,该研究首先提出一种基于分组激活策略的分组注意力模块,利用高阶特征指导加强低阶特征,通过分组计算组内加强系数,减少不同组之间的抑制作用,加强特征表达能力。对比试验表明,分组注意力模块特征强化效果优于传统注意力模块。基于分组注意力模块,该研究提出一种实时高效农作物病害叶片语义分割模型,该模型融合了编码-解码语义分割模型和多流语义分割模型的优点。采用ResNet18模型作为特征提取网络对农作物病害叶片的语义分割像素精度达到93.9%,平均交并比达到78.6%。在单张NVIDIA GTX1080Ti显卡的硬件环境下,输入分辨率为900×600像素的图片,该模型运行速度达到每秒130.1帧,满足实时农作物病害叶片语义分割需求,为现代农业病害识别、自动施肥和精准灌溉等应用提供参考。     

结构域Tt APuX21基因的分离与克隆研究

以热产硫化氢高温厌氧杆茵茵液为模板,根据Gene Bank中登记的编码结构域Tt APuX21基因序列设计1对特异引物,利用多聚酶链反应技术,扩增出目的基因Tt apux21,并克隆到表达栽体pET21a( )中.经茵液PCR筛选和DNA测序鉴定.表达栽体pET21a( )中插入有序列正确的Tt apux21基因,重组质粒命名为pEX21.     

Inhibition of miR397 by STTM technology to increase sweetpotato resistance to SPVD

As a critical food crop, sweetpotato(Ipomoea batatas(L.) Lam.) is widely planted all over the world, but it is deeply affected by Sweetpotato Virus Disease(SPVD). The present study utilized short tandem target mimic(STTM) technology to effectively up-regulate the expression of laccase(Ib LACs) by successfully inhibiting the expression of mi R397. The upstream genes in the lignin synthesis pathway were widely up-regulated by feedback regulation, including phenylalanine ammonialyase(PAL), 4-coumar...     

葡萄miR159及其靶基因VvGAMYB在花发育过程中的作用分析

以‘魏可’葡萄(Vitis vinifera L.‘Wink’)为试材,克隆得到一个葡萄赤霉素信号途径中的MYB转录因子基因Vv GAMYB。该基因开放阅读框(ORF)长1 680 bp,编码559个氨基酸,该蛋白序列含有GAMYB基因家族保守的R2R3 DNA结合域以及Box1、Box2、Box3保守域。洋葱表皮细胞的瞬时表达结果显示,VvGAMYB定位于细胞核中。VvGAMYB和其下游基因Vv LFY在葡萄花发育过程中的表达都呈现先上升后下降的趋势,而赤霉素处理促进了VvGAMYB和VvLFY的表达,从而使开花提前。VvGAMYB是miR159的靶基因,其作用受到miR159的调控。miR159和VvGAMYB的表达负相关,赤霉素处理下调miR159表达从而也降低了对VvGAMYB的裂解作用。由上述结果可以看出,VvGAMYB通过赤霉素开花途径参与葡萄的开花过程。在这一过程中,赤霉素抑制miR159裂解VvGAMYB的作用,从而上调VvLFY的基因表达,参与了调控葡萄开花过程。     

MiR‐222 inhibits apoptosis in porcine follicular granulosa cells by targeting the THBS1 gene

Apoptosis of granulosa cells affects follicular atresia and reproduction and is regulated by miRNAs and the expression of certain genes. For the present study, we investigated the regulatory relationship between microRNA‐222 (miR‐222) and THBS1 in porcine follicular granulosa cells (pGCs) and its effects on apoptosis to provide empirical data for developing methods to improve pig fecundity. Results revealed that miR‐222 promotes the proliferation of pGCs. MiRNA mimics and luciferase reporter assays revealed that miR‐222 functions as an anti‐apoptotic factor in pGCs. MiR‐222 mimics in pGCs result in the upregulation of the anti‐apoptotic BCL‐2 gene, down‐regulation of the proapoptotic caspase‐3 gene, and inhibition of apoptosis. MiR‐222 inhibitors reduced BCL‐2 and had no significant effect on caspase‐3. MiR‐222 mimics promoted estrogen levels. Inhibition of THBS1 inhibited pGC apoptosis. Transfection of THBS1‐siRNA reduced the proapoptotic BAX gene. MiR‐222 can directly target the 3′‐untranslated region of the THBS1 gene. MiR‐222 mimics suppressed THBS1 mRNA and proteins, but these were upregulated by the miR‐222 inhibitor. Transfection of THBS1‐siRNA resulted in the inhibition of the miR‐222 inhibitor, which suggests that miR‐222 inhibits pGC apoptosis by targeting THBS1. These findings suggest that miR‐222 and THBS1 play important roles in follicular atresia, ovarian development, and female reproduction.     

基于单片机的多功能定时打铃钟设计

为了解决部分学校传统打铃钟操作不便,静态电流大,电路复杂,可靠性低,成本高的问题,文章中设计的系统采用单片机AT89C51和移位寄存器74LS164为核心,通过驱动电路实现打铃的可显示自动走时的多功能定时打铃钟。文章详细介绍了系统总体方案及部分硬件设计方案。     

新农村建设住宅结构体系

新农村建设过程中,住宅结构体系的研究开发至关重要。该研究对当前农村常见多层住宅结构体系进行了分析和比较,重点介绍了保温模块剪力墙住宅体系所具备的优势,指出了保温模块剪力墙住宅体系是符合多层住宅结构体系发展方向的住宅结构体系。     

红外线计数器的设计与制作

用一体化红外线传感器TX05D及十进制计数/7段译码器二合一集成电路CD4033等器件,制作了性能优良的红外线计数器。该红外线计数器的特点是:抗干扰能力强,工作稳定可靠;计数范围为1～999999个数,高亮度数字显示,可用于工农业一些设备或流水线上对产量或流量进行统计计数。     

智能低压无功补偿技术在低压电网中的应用

本文通过对无功补偿技术的回顾，结合现阶段无功补偿设备的具体情况，集中阐述了智能低压无功补偿设备在低压配电网中的应用。