环斑花叶病侵染对番木瓜叶片呼吸作用和叶绿体膜脂的影响

环斑花叶病侵染的番木瓜叶片暗呼吸速率为1.85μmol·O_2·dm~(-2)·min~(-1),较健叶增高63.7％,但光下依赖CO_2的氧释放速率较健叶低33.6％。表明环斑花叶病侵染叶片引起暗呼吸增高和抑制光下依赖CO_2的氧释放。在低光强(低于0.2m mol光量子·m~(-2)·S~(-1))病叶的氧释放速率(RO,μmol·O_2·dm~(-2)·min~(-1))为RO=-0.07+2.38PFD,而健叶的相应关系为RO=1.08+2.43PFO。表明氧释放速率与光强关系曲线的最初直线斜率,健叶的较病叶的高;氧释放速率随光强的增高而上升得较快。环斑花叶病侵染亦引起叶绿体膜脂半乳糖甘油脂和酰脂相对含量降低。病原的浸染可能引起叶绿体膜脂部分降解。     

乳状液膜分离氨基酸的研究进展

综述了乳状液膜技术的基本原理及其在分离提取氨基酸方面的研究进展状况。     

嗜水气单胞菌表层蛋白及外膜蛋白的初步分析

用甘氨酸缓冲液处理对鱼类致病的嗜水气单胞菌代表菌株 Ah J-1菌体细胞,可使该菌表层蛋白(S层)成片脱落。电镜观察发现,S 层是由四边形的蛋白亚单位呈晶格样排列而成。十二烷基磺酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)分析表明,该蛋白的分子量约50k;免疫印迹显示,它是 Ah J-1的主要表面抗原。另将 Ah J-1用 Triton X-100选择性抽提,经 SDS-PAGE,在考马斯亮蓝染色图谱中显示其外膜蛋白(OMP)为3条主要蛋白带,分子量分别为34,46和58k.     

镉对小鼠肝线粒体膜流动性及呼吸活性的影响

采用１，６－二苯基－１，３，５－己三乙烯（ＤＰＨ）荧光偏振法研究了镉在小鼠体内对肝线粒体膜流动性的影响，同时观察了膜结合酶活性和线粒体呼吸功能的变化。结果表明，镉能显著增加小鼠肝线粒体膜的荧光偏振度（Ｐ）及微粘度（η），并导致线粒体呼吸控制比（ＲＣＲ）和氧化磷酸化效率（ＡＤＰ／Ｏ）显著下降。膜结合Ｋ＋、Ｎａ＋－ＡＴＰ酶、Ｃａ２＋－ＡＴＰ酶、Ｍｇ２＋－ＡＴＰ酶和琥珀酸脱氢酶（ＳＤＨ）的活性均受到明显抑制。实验结果提示硒能有效地保护镉所诱导的这些损伤变化     

鸡沙门氏菌外膜蛋白亚单位疫苗的研究——Ⅱ油乳剂型(OE)亚单位疫苗的研究

用十二烷酰肌氨酸法提取鸡沙门氏菌外膜蛋白，作为抗原，制备油乳剂型（Oil Emulsion OE)亚单位疫苗。该亚单位疫苗为油包水（W/O）型，稳定性良好，安全可靠。对3周龄雏鸡用该疫苗肌注接种，6周龄攻毒，结果表明，接种800ugOMP/只油乳剂型亚单位疫苗的雏鸡对鸡白痢沙门氏菌或鼠伤寒沙门氏菌的攻击具有显著的免疫保护力。     

炼油厂氨氮废水的乳状液膜法处理研究

采用正交试验设计,首次利用混合表面活性剂,研究了乳状液膜法对炼油厂氨氮液的处理效果及其影响因素。实验结果表明:以22 %硫酸为内相,4 %Span-80+2 %T152+煤油+11 %的液体石蜡为液膜组成,pH=11的废水为外相的处理体系,处理效果可达到99.8 %,并在实际应用中取得了满意的效果。     

应用激素提高母牛受配率的试验报告

几年来，我们应用三合激素、氯前列烯醇及氯前列稀醇配合孕马血清处理１５６头乏情母牛，结果３组的催经分别为８４．８％、７９．５％、８６．９％，同时配种后第一期受胎率分别为４．４％、３２．２％和４８．４％。试验表明第三组的催情率和受胎率都比较好，是提高母牛受配率的有效方法之一。     

苹果链格孢菌侵染对感病苹果品种叶细胞超微结构的影响

 将Alternaria mali分生孢子接种在苹果元帅品种叶片的下表皮,保湿培养3~24h,观察细胞超微结构,接种后3h,表皮及叶肉细胞的质膜发生内陷,出现囊泡,暗染色颗粒及管状结构,叶绿体膜结构解体,电子致密物质沉积,基粒排列混乱松散,外膜破裂等。接种后24h,叶片表面出现病斑,细胞质浓缩,细胞壁出现颗粒物,细胞变形,病健组织交界处,叶绿体基浓缩,出现脂质球,同时还观察到,距离病斑2mm处,许多叶肉细胞中出现密度均匀、形状不规则的物质,小叶脉木质部中出现电子致密物质沉积。     

A morphologic study of intravitreal membranes associated with intraocular hemorrhage in the dog

Abstract We aimed to characterize intravitreal membranes in dogs and determine, if possible, associated predisposing conditions. Five globes in which intravitreal membranes were identified were evaluated. These originated from four Labrador Retrievers or Labrador-cross dogs and a Springer Spaniel. The ages of the dogs ranged from 4 to 11 years. Standard histology and immunohistochemical procedures for factor VIII-related antigen, smooth muscle actin (SMA), vimentin and glial fibrillary acidic protein (GFAP) were performed. Intravitreal membranes varied from loosely to highly organized. The extent of organization corresponded with increasing immunoreactivity for vimentin and GFAP, indicating their predominantly glial origin. They were never immunopositive for smooth muscle actin, nor were they vascularized. In all cases, they were associated with intravitreal hemorrhage. Additional common findings included epiretinal membranes, retinal neovascularization, preiridal fibrovascular membranes and glaucoma. Intravitreal membranes may be a sequelae of intravitreal hemorrhage. This in turn, may arise from new vessels associated with epiretinal or preiridial membranes, or hemorrhage associated with optic disc cupping or retinal neovascularization. All of these phenomena may accompany glaucoma.     

Amniotic membrane transplantation for the reconstruction of the ocular surface in three cases

This paper describes the use of amniotic membrane in the reconstructive surgical repair of generalized keratomalacia, ankyloblepharon, and after fibrous histiocytoma removal in two dogs and a cat. Case 1 was an 11-year-old female Yorkshire terrier with severe bullous keratomalacia in the left eye (OS). A frozen canine amniotic membrane graft and a third eyelid flap were applied. At day 80 postoperatively, only a mild scar and corneal vascularization were present. Case 2 was a 4-year-old female Siamese cat with symblepharon of both eyes after rhinotracheitis. Resection of the conjunctiva and frozen canine amniotic membrane transplant were performed. One month later, there was a little corneal scarring and corneal vascularization. Case 3 was a 6-year-old female terrier with a scleral and corneal mass at the 11-12 o'clock position (OS). Resection of the mass and amniotic membrane transplantation were performed. The mass was a fibrous histiocytoma localized to the sclera and cornea. This eye healed with mild conjunctivalization and no pigmentation. Amniotic membrane transplantation can be used as a method of reconstruction of the ocular surface with good repair of the cornea and minimal scarring in small animals.