长期有机无机肥配施对土壤水稳性团聚体酶活性的影响

The activities of invertase, protease, urease, acid phosphomonoesterase, dehydrogenase, and catalase in different fractions of waterstable aggregates (WSA) were examined in long-term (26 years) fertilised soils. The long-term application of organic manure (OM) with chemical fertiliser (CF) significantly increased macroaggregate and decreased microaggregate percentages, enhanced the mean weight diameter, and significantly increased soil total carbon (TC) and total nitrogen (TN) contents of WSA in different size fractions. Combined fertilisation with OM and CF also increased invertase, protease, urease, acid phosphomonoesterase, dehydrogenase, and catalase activities of WSA in different size fractions. Enzyme activities were higher in macroaggregates than in microaggregates. The distribution of enzyme activities generally followed the distribution of TC and TN in WSA. The geometric mean of the enzyme activities in different WSA of OM-treated soils was significantly higher than that in soils treated with 100% CF or no fertiliser. The results indicated that the long-term combined application of OM with CF increased the aggregate stability and enzyme activity of different WSA sizes, and consequently, improved soil physical structure and increased soil microbial activity.     

《植物组织培养》课程教学内容和方法改革的探索与实践

介绍了《植物组织培养》课程教学内容和方法。首先根据课程教学目标和高职教育人才培养要求确定改革的基本思路;根据职业岗位要求,结合毕业生反馈的信息精心组织教学内容,以器官培养为主线讲述基本概念、基本原理和基本技术;在教学过程中,注重将理论与实践相结合,讲授、操作与讨论相结合,单项操作与综合训练相结合,操作训练与实践考核相结合,启发学生思维,引导学生进行创新;充分利用多媒体课件教学。     

基于网页的SBE法研究广州城市公园木本花卉景观

通过对广州市公园木本花卉景观的调查和拍摄相关植物景观照片,采用研究自然风景质量评价的心理物理学方法中的评分法——SBE法(Scenic Beauty Estimation Procedures),让公众通过网络的方式,以网页为媒介对木本花卉景观进行评价;通过对评价数据的标准化处理和SAS系统的数据分析,寻找木本花卉景观的最佳配置方式,为该类植物景观的营建提供科学的依据,并探索一条快速、简便而有效的景观评价模式。     

煤矿监控系统备用电源的可靠性预计

首先介绍了可靠性的概念特征、可靠性模型的建立和可靠性预计方法。根据CJB／Z299B-98标准中的可靠性预计方法,对煤炭安全生产监控系统的备用电源进行了可靠性预计,得到了在高温150℃电应力系数小于0．3时的平均无故障时间。     

山东沿海褶牡蛎与太平洋牡蛎等位基因酶的遗传变异

采用水平淀粉凝胶电泳技术研究了山东沿海莱州 (LZ)、俚岛 (LD)和青岛 (QD)三地褶牡蛎自然群体以及荣成的太平洋牡蛎 (TP)养殖群体的等位基因酶遗传变异。进行测试的 9种酶中共检测到 2 0个位点。LZ与QD群体的多态位点比例为 6 0 .0 % ,其余两群体均为 5 5 .0 %。LZ、LD、TP和QD各位点平均有效等位基因数分别为 1.42 2± 0 .16 5、1.2 38± 0 .0 85、1.2 0 7± 0 .0 86和 1.32 6± 0 .119。平均杂合度观察值分别为 0 .2 16± 0 .0 5 0、0 .16 5± 0 .0 36、0 .139± 0 .0 34和 0 .195± 0 .0 42。结果表明 ,LZ和QD群体的遗传变异水平高于LD和TP群体。同时 ,各群体中普遍存在杂合子缺失现象。太平洋牡蛎群体与其它三个褶牡蛎群体的遗传距离很小 ,甚至小于莱州和青岛的两个褶牡蛎群体间的遗传距离 0 .0 5 0 0 ,尤其是与俚岛褶牡蛎群体遗传相似度最大 ,遗传距离小于其它各群体之间的距离 ,仅为 0 .0 0 99。     

山地丘陵区不同土地利用方式对空心村整治还田土壤团聚体特征的影响

以探明不同土地利用方式对土壤团聚体的影响为目的,为提高山地丘陵区空心村整治还田土壤稳定性及生产性能提供科学依据。在陕西澄县山地丘陵区空心村整治还田后,设置5种不同的土地利用方式,开展为期1年的种植试验,分别为玉米(C处理)、小麦(W处理)、蔬菜(V处理)、药材(M处理)及对照(未种:CK处理)。测定分析干筛法和湿筛法0—40cm土层土壤团聚体分布、平均质量直径(WMD)、几何平均直径(GMD)、团聚体破坏率(PAD)、不稳定团粒数(ELT)和分形维数(D)。结果表明:(1)各处理在0—40cm各土层土壤团聚体数量及大小均显著优于CK处理,各处理干筛下0—40cm土层0.25mm团聚体含量(DR0.25)和湿筛下该含量(WR0.25)随着土壤土层深度的增加呈现相反的趋势;(2)干筛法和湿筛法0—40cm土层各处理土壤平均重量直径(MWD)平均值和几何平均直径(GMD)平均值大小顺序均呈现W处理C处理M处理V处理CK处理,C处理有助于增加表层土壤大团聚体含量,W处理则有助于增加下层土壤大团聚体含量;(3)湿筛法分析表明,各处理土壤团聚体破坏率(PAD)和不稳定团粒指数(ELT)在0—40cm土层内均表现出近似"Z"字形趋势,各处理显著低于CK;(4)各处理的分形维数(D)在0—40cm土层平均值大小顺序为C处理W处理M处理V处理CK处理。土壤分形维数(D)与干筛法和湿筛法下0—40cm土层0.25mm团聚体含量之间存在良好的线性关系,分别为R2=0.74和R2=0.67。空心村整治还田后种植玉米和小麦有利于提高0—40cm土层大团聚体含量,增加土层稳定性,改善土壤结构。     

农田土壤碳氮及其与气象因子的关系

为揭示土壤碳氮受外界环境因子的影响,基于辽宁省51个农业气象站的代表性,分析了土壤有机碳和全氮的剖面分布及其与气象因子、地理因子的关系。结果表明,土壤表层（0~20 cm）有机碳和全氮含量总体高于土壤下层（20~40 cm）;不同土层土壤有机碳和全氮受年降水量、年平均气温的影响程度不同。土壤表层有机碳和全氮受年降水量、年平均气温的显著或极显著影响（P〈0.05,P〈0.01）;土壤下层有机碳和全氮均与年平均气温呈显著负相关（P〈0.01）,而与年降水量不成显著相关。经度与土壤表层有机碳和土壤下层全氮呈显著正相关（P〈0.05）,而纬度和海拔不影响土壤有机碳和全氮。不同土层有机碳、全氮与气象因子的回归方程为农田生态系统的碳收支评估及养分循环提供基础资料。     

Cultivation effects on soil texture and fertility in an arid desert region of northwestern China

In arid desert regions of northwestern China, reclamation and subsequent irrigated cultivation have become effective ways to prevent desertification, expand arable croplands, and develop sustainable agricultural production. Improvement in soil texture and fertility is crucial to high soil quality and stable crop yield. However, knowledge on the long-term effects of the conversion of desert lands into arable croplands is very limited. To address this problem, we conducted this study in an arid desert region of northwestern China to understand the changes in soil physical-chemical properties after 0, 2, 5, 10, 17, and 24 years of cultivation. Our results showed that silt and clay contents at the 17-year-old sites increased 17.5 and 152.3 folds, respectively, compared with that at the 0-year-old sites. The soil aggregate size fraction and its stability exhibited an exponential growth trend with increasing cultivation ages, but no significant change was found for the proportion of soil macroaggregates (>5.00 mm) during the 17 years of cultivation. The soil organic carbon (SOC) content at the 24-year-old sites was 6.86 g/kg and increased 8.8 folds compared with that at the 0-year-old sites. The total (or available) nitrogen, phosphorus, and potassium contents showed significant increasing trends and reached higher values after 17 (or 24) years of cultivation. Changes in soil physical-chemical properties successively experienced slow, rapid, and stable development stages, but some key properties (such as soil aggregate stability and SOC) were still too low to meet the sustainable agricultural production. The results of this long-term study indicated that reasonable agricultural management, such as expanding no-tillage land area, returning straw to the fields, applying organic fertilizer, reducing chemical fertilizer application, and carrying out soil testing for formula fertilization, is urgently needed in arid desert regions.     

海南水稻生育期的时空变化特征及对气候变暖的响应

基于海南6个农业气象观测站点的水稻生育期资料和1961~2014年历史气象数据,分析了海南不同地区水稻生育期的时空分布特征,以及气候变暖对海南水稻生育期的影响。结果表明,近54 a海南水稻生长季内平均气温呈上升趋势,早稻气候倾向率为0.21~0.3℃/10 a,以苗期最为明显,晚稻为0.18~0.24℃/10 a,以成熟期最为明显。不同地区水稻生育期差异较大,东部地区早于西部地区,早稻播种时间最大相差55 d,早稻收获和晚稻播种最大间隔42 d,降水和高温可能是造成这一差异的主要原因。随着气候变暖,海南大部分地区早稻生育期提前,晚稻生育期延迟,苗期和成熟期持续时间缩短,与该阶段平均气温呈负相关,但全生育期持续时间变异较大,部分站点略有延长。在未来气候变暖背景下,海南早稻播期可适当提前,晚稻播期可适当延迟,并选用耐高温和抗干旱品种,以减轻高温的不利影响。