6株金针菇菌株遗传多样性的ISSR、RAPD和SRAP综合分析

为了更加快速准确的分析金针菇种质资源的遗传多样性,利用ISSR、RAPD和SRAP分子标记技术对6株金针菇菌株进行综合遗传多样性分析,并构建UPMGA亲缘关系树状图。结果表明,6株金针菇菌株间的遗传相似系数范围为0.49~0.85,在相似系数为0.59时6个菌株可分为3组,黄色菌株‘三明1号’、‘F951’、‘杂19’和白色菌株8801聚成一组,白色菌株8903和白色菌株‘金21’各成一组。该试验结果表明,3类分子标记综合分析结果相比单一分子标记分析结果能够更真实全面地体现出供试菌株之间的亲缘关系,因此该方法将有利于金针菇种质资源的评价和鉴定研究。     

运用广义线性混合模型分析随机区组重复测量的试验资料

重复测量试验对同一受试对象进行多次测量,各时间点数据间存在自相关性,进行方差分析和均值比较时需要进行特殊处理。虽然此方法在农业等研究领域运用十分广泛,但目前有效地相关统计方法鲜见。为了建立操作简单、实用性强、结果可靠的统计分析方法,本研究采用SAS的广义线性混合模型(Generalized Linear Mixed Models,GLIMMIX),以随机区组重复测量试验资料为例,说明了协方差结构筛选、方差分析和均值比较的具体方法。结果表明,用传统的裂区设计、多变量统计等方法会造成资料信息浪费,统计功效降低,缺区无法处理等问题,甚至会导致错误的结论。GLIMMIX能很好地处理自相关问题,功能强大,结果可靠,使用简单,允许缺区,是进行重复测量试验资料方差分析和均值比较的理想方法。目前在国内将其运用到农学类试验数据的统计分析的相关报道鲜见,该文在本领域具有很强的实用性和创新性。     

The effect of aqueous extract of gross and commercial yerba mate (Ilex paraguariensis) on intra-abdominal and epididymal fat and glucose levels in male Wistar rats

This study analyzed the plasma lipid profile, glucose levels and fat deposits in male rats treated with aqueous extract of gross yerba mate, commercial yerba mate or water. Yerba mate treatment did not change body weight gain and lipid profile. The consumption of gross yerba mate significantly increased blood glucose (6.6 mmol/L) as compared to the water (4.8 mmol/L) and commercial group (5.2 mmol/L) and decreased epididymal and intra-abdominal deposits (10.1 mg/g and 23.7 mg/g of weight) as compared to the water (15.4 mg/g and 36.9 mg/g of weight) and commercial group (12.5 mg/g and 28 mg/g of weight). The results suggest that gross yerba mate reduces fat more efficiently but produces a greater increase in blood glucose when compared to commercial yerba mate and water groups.     

Variations in carbon and nitrogen ratios and their effects on seed germination in Cupressus sempervirens populations

This study investigated the variations in the carbon (C), nitrogen (N), and C/N ratios in the seeds of the Mediterranean Cypress (Cupressus sempervirens L.), a typical forest tree of the Mediterranean Region. The data were gathered from populations distributed across Turkey. We also evaluated the relationship between these variations and germination parameters such as the germination percentage (GP) and mean germination time (MGT). All of the evaluated characteristics demonstrated statistically significant variations among the populations. The GPs were generally low and varied between 14% in Datça and 51% in the Köprülü Kanyon. The MGTs ranged from 14.9 in the Köprülü Kanyon to 18.9 in Marmaris. In addition, the C ratios varied between 46% in Marmaris and 52% in the Köprülü Kanyon, while the N ratios ranged from 1.1% in Beycik and Göksu to 1.8% in the Köprülü Kanyon. Furthermore, we observed a positive correlation between the GP and the C and N contents (R² = 0.51 and R² = 0.49, respectively) but found a negative correlation between the MGT and the C and N contents (R² = 0.56 and R² = 0.32, respectively). Moreover, with regard to the combined C and N seed ratios of the regression models, R² = 0.95 for the GP and R² = 0.87 for the MGT, and the populations with higher quantities of C and N in their seeds had shorter MGTs and higher GPs.     

Tentative Research About the Macroscopic Regulating to Blast

The paper analyzes the difference of the pathogenic varieties and the relationship of the variety composition,allording to the historic material to the varieties of the main hybid fece varieties in Southeast Sichuan during 1992 to 1998.It's the first time to advance to use the camparison on rate of pathogenic isolates between norm and refrieving to define the susceptible extent of the varity.It's an scientific base to adjust the structures of the vartieties,to reach the objective of the macroscopic regulating to rice blast.     

ECMWF细网格对浙江沿海10 m风预报性能评估

为更好地开展浙江沿海海上大风的预报服务,本研究对2012年1月1日—2014年12月31日的ECMWF细网格10 m风场产品在浙江沿海的预报性能进行评估,并将其插值到沿海站点和观测数据进行对比分析。结果表明:冷空气影响下,浙江沿海的平均误差为负值,绝对误差分布和变化趋势与平均误差基本一致;台风影响下,24 h预报时效的平均误差为正值,随着预报时效的增加,平均误差逐渐转为负值,鱼山渔场、温台渔场及舟外渔场的绝对误差较其他区域大。另外,预报值和观测数据间的相关系数随预报时效的增加而减小,两者之间的相关系数平均值随海拔高度的增加而减小;ECMWF细网格对岱山和龙山村的预报偏大,对浪岗的预报偏小,预报偏差的离散度随预报时效增加而增大。     

水科学中应用数理统计方法应注意

对回归方程误差的影响因素进行了分析，导出了回归方程的均方误差与其影响因素之间的关系式。分析表明，复相关系数(或相关系数)一定时，倚变量的变差系数越大，回归方程的均方误差就越大；回归分析时，应对回归方程的误差进行分析。对于样本均值估计总体均值，给出了基于误差限制的样本容量的计算式。研究表明，应考虑样本变差系数对估计精度的影响。     

Simulation of transpiration, drainage, N uptake, nitrate leaching, and N uptake concentration in tomato grown in open substrate

Free-drainage or “open” substrate system used for vegetable production in greenhouses is associated with appreciable NO₃⁻ leaching losses and drainage volumes. Simulation models of crop N uptake, N leaching, water use and drainage of crops in these systems will be useful for crop and water resource management, and environmental assessment. This work (i) modified the TOMGRO model to simulate N uptake for tomato grown in greenhouses in SE Spain, (ii) modified the PrHo model to simulate transpiration of tomato grown in substrate and (iii) developed an aggregated model combining TOMGRO and PrHo to calculate N uptake concentrations and drainage NO₃⁻ concentration. The component models simulate NO₃⁻-N leached by subtracting simulated N uptake from measured applied N, and drainage by subtracting simulated transpiration from measured irrigation. Three tomato crops grown sequentially in free-draining rock wool in a plastic greenhouse were used for calibration and validation. Measured daily transpiration was determined by the water balance method from daily measurements of irrigation and drainage. Measured N uptake was determined by N balance, using data of volumes and of concentrations of NO₃⁻ and NH₄⁺ in applied nutrient solution and drainage. Accuracy of the two modified component models and aggregated model was assessed by comparing simulated to measured values using linear regression analysis, comparison of slope and intercept values of regression equations, and root mean squared error (RMSE) values. For the three crops, the modified TOMGRO provided accurate simulations of cumulative crop N uptake, (RMSE = 6.4, 1.9 and 2.6% of total N uptake) and NO₃⁻-N leached (RMSE = 11.0, 10.3, and 6.1% of total NO₃⁻-N leached). The modified PrHo provided accurate simulation of cumulative transpiration (RMSE = 4.3, 1.7 and 2.4% of total transpiration) and cumulative drainage (RMSE = 13.8, 6.9, 7.4% of total drainage). For the four cumulative parameters, slopes and intercepts of the linear regressions were mostly not statistically significant (P < 0.05) from one and zero, respectively, and coefficient of determination (r²) values were 0.96-0.98. Simulated values of total drainage volumes for the three crops were +21, +1 and −13% of measured total drainage volumes. The aggregated TOMGRO-PrHo model generally provided accurate simulation of crop N uptake concentration after 30-40 days of transplanting, with an average RMSE of approximately 2 mmol L⁻¹. Simulated values of average NO₃⁻ concentration in drainage, obtained with the aggregated model, were −7, +18 and +31% of measured values.     

水匮乏指数在区域水安全评价中的应用

针对水安全问题,以农垦红兴隆分局为例,采用水匮乏指数和样本均值-标准差分级法对红兴隆分局下属12个农场2006年的水安全状况进行了评价和分级,并根据分级结果提出了相应的对策。结果表明,八五三和红旗岭农场的WPI值处于安全状态,水安全状况最为乐观;饶河农场的WPI值处于较高安全状态,水安全状况较好;友谊、五九七、八五二、二九一和江川农场的WPI值处于中等安全状态,水安全保障体系有待于进一步加强;北兴农场的WPI值处于较低安全状态,水安全状况较差;而双鸭山、曙光和宝山农场的WPI值处于不安全状态,水安全状况最差。     

质量—数量性状的遗传分析 Ⅲ.受三倍体遗传控制的胚乳性状

将二倍体质量—数量性状的遗传分析方法推广应用于受三倍体遗传控制的胚乳性状。给出了加性—显性模型下有关胚乳世代的平均数和遗传方差的分量。以稻米胚乳的糊化温度（以碱扩散值表示,简记为ASV）为例演示了分析程序。结果表明被研究组合ASV的遗传涉及一个主基因和基干微基因。根据分离世代中胚乳ASV的株平均数和方差,可