全文获取类型
收费全文 | 1468篇 |
免费 | 73篇 |
国内免费 | 73篇 |
专业分类
林业 | 95篇 |
农学 | 112篇 |
基础科学 | 9篇 |
263篇 | |
综合类 | 639篇 |
农作物 | 90篇 |
水产渔业 | 63篇 |
畜牧兽医 | 174篇 |
园艺 | 69篇 |
植物保护 | 100篇 |
出版年
2024年 | 4篇 |
2023年 | 11篇 |
2022年 | 18篇 |
2021年 | 34篇 |
2020年 | 23篇 |
2019年 | 35篇 |
2018年 | 19篇 |
2017年 | 45篇 |
2016年 | 56篇 |
2015年 | 41篇 |
2014年 | 42篇 |
2013年 | 120篇 |
2012年 | 106篇 |
2011年 | 106篇 |
2010年 | 82篇 |
2009年 | 82篇 |
2008年 | 69篇 |
2007年 | 63篇 |
2006年 | 67篇 |
2005年 | 67篇 |
2004年 | 47篇 |
2003年 | 40篇 |
2002年 | 35篇 |
2001年 | 35篇 |
2000年 | 35篇 |
1999年 | 33篇 |
1998年 | 37篇 |
1997年 | 28篇 |
1996年 | 32篇 |
1995年 | 15篇 |
1994年 | 18篇 |
1993年 | 27篇 |
1992年 | 22篇 |
1991年 | 18篇 |
1990年 | 28篇 |
1989年 | 19篇 |
1988年 | 11篇 |
1987年 | 9篇 |
1986年 | 8篇 |
1985年 | 3篇 |
1984年 | 2篇 |
1983年 | 2篇 |
1982年 | 3篇 |
1981年 | 1篇 |
1980年 | 1篇 |
1979年 | 4篇 |
1978年 | 4篇 |
1977年 | 5篇 |
1973年 | 1篇 |
1955年 | 1篇 |
排序方式: 共有1614条查询结果,搜索用时 15 毫秒
1.
采用激光诱导击穿光谱(LIBS)技术并结合在锌基板上液固转换的样品处理方法,对标准水溶液样品中Fe、Mn进行检测,并根据标准曲线法建立Fe、Mn的定量模型;再利用所建立的LIBS定量模型,对洞庭湖区地下水实际水样中Fe、Mn含量进行检测,并与电感耦合等离子体质谱法(ICP–MS)的检测结果进行比较。结果表明:得到Fe、Mn的LIBS定标曲线的决定系数均大于0.98,相对标准偏差均为4.3%,留一交叉验证均方根误差分别为0.024、0.007 mg/L,Fe、Mn的检出限分别为0.028、0.008 mg/L;采用LIBS对洞庭湖地下水实际水样中Fe、Mn含量检测的结果与ICP–MS的检测结果基本一致,平均相对误差小于10%。 相似文献
2.
杨盘二孢激发子的分离及稳定性研究 总被引:1,自引:0,他引:1
从杨盘二孢的培养滤液和菌丝中获得2种激发子粗提物,分别测定其糖和蛋白质的含量,发现滤液激发子粗提物(CFE)糖和蛋白质的含量分别为41.07和40mg/mL,菌丝激发子粗提物(CME)糖和蛋白质含量分别为48.07和55mg/mL。滤液激发子粗提物对温度不敏感而对碱性条件敏感;菌丝激发子粗提物对酸碱不敏感而对温度敏感。用Sephadex G-100柱层析的方法初步纯化2种激发子,并且菌丝激发子粗提物过柱后得到2个活性组分J14和J25;滤液激发子粗提物过柱后获得2个活性组分L9和L16。将4个组分进行烟草叶片过敏反应和I-895杨酶活性的诱导,结果表明,菌丝激发子强活性物质集中在J14组分;而滤液激发子强活性物质集中在L9组分。 相似文献
3.
《Soil Science and Plant Nutrition》2013,59(1):26-31
Abstract The present study was undertaken to identify the effects of manganese (Mn) on the activity of peroxidase (PO) and the amount of ascorbic acid (AsA) and lignin, as well as the cell viability of suspension-cultured tea cells (Camellia sinensis L. cv. Yabukita). Cells were grown in B5 medium (containing 0.06 mmol L?1 Mn) and cultured for 24 h in medium with a Mn concentration of 0.9 mmol L?1 as an excess treatment. No significant difference was observed between cellular growth and the viability of the cultured tea cells after treatment with excess Mn compared with cells grown under control conditions, although the content of Mn in cells in the excess Mn treatment was 12-fold higher than that of the control cells. The amount of total AsA was also not affected by the Mn treatment. The activity of ionically wall-bound peroxidase (IPO) increased in the presence of excess Mn, unlike the content of lignin. Conversely, the activities of soluble PO and covalently wall-bound PO decreased with excess Mn. These findings suggested that IPO might contribute to Mn tolerance in tea cells. However, its role in the mechanism(s) of Mn tolerance has not been elucidated. 相似文献
4.
5.
6.
(Jpn. J. Soil Sci.Plant Nutr., 77, 257–263, 2006)
The symptom of blighted leaves was observed for the melon plants grown on an isolated soil bed after steam sterilization (SS) in a greenhouse in Kochi Prefecture, Japan. To clarify the causes of this symptom, soil and plant analyses were conducted in the first experiment. Manganese concentrations in the leaves with the symptom were higher than 1,000 mg kg−1and exchangeable Mn in the soil was higher than 30 mg kg−1, which indicated the observed symptom was due to Mn excess. In the second experiment, formation of Mn oxide-dissolving substances after SS was examined for several organic amendments, because large amounts of palm chips had been supplied to the soil before SS in the first experiment. As a treatment similar to SS, palm chips, bark compost, and cattle feces manure were autoclaved. The water extracts were collected before and after the autoclave treatment and the Mn oxide-dissolving capacities of the extracts were evaluated. The Mn oxide-dissolving capacity of the water extracts after SS was remarkably higher than before SS, and the value was highest in palm chips, and followed by bark compost, and cattle feces manure. Fractionation and HPLC analysis of the water extracts after SS revealed that the main substance behind Mn oxide reduction in palm tips was arabinose and that in bark manure was malic acid. Further, Mn oxide-dissolving capacities of the water extracts were almost completely explained by the amounts of arabinose or malic acid found in these materials. From these results, it was considered that reducing substances such as arabinose were released by SS from the palm chips which had been applied in large quantity before SS and these reducing substances dissolved the soil Mn oxides and increased the amounts of available Mn, which led to the occurrence of Mn toxicity to the plants. 相似文献
The symptom of blighted leaves was observed for the melon plants grown on an isolated soil bed after steam sterilization (SS) in a greenhouse in Kochi Prefecture, Japan. To clarify the causes of this symptom, soil and plant analyses were conducted in the first experiment. Manganese concentrations in the leaves with the symptom were higher than 1,000 mg kg−1and exchangeable Mn in the soil was higher than 30 mg kg−1, which indicated the observed symptom was due to Mn excess. In the second experiment, formation of Mn oxide-dissolving substances after SS was examined for several organic amendments, because large amounts of palm chips had been supplied to the soil before SS in the first experiment. As a treatment similar to SS, palm chips, bark compost, and cattle feces manure were autoclaved. The water extracts were collected before and after the autoclave treatment and the Mn oxide-dissolving capacities of the extracts were evaluated. The Mn oxide-dissolving capacity of the water extracts after SS was remarkably higher than before SS, and the value was highest in palm chips, and followed by bark compost, and cattle feces manure. Fractionation and HPLC analysis of the water extracts after SS revealed that the main substance behind Mn oxide reduction in palm tips was arabinose and that in bark manure was malic acid. Further, Mn oxide-dissolving capacities of the water extracts were almost completely explained by the amounts of arabinose or malic acid found in these materials. From these results, it was considered that reducing substances such as arabinose were released by SS from the palm chips which had been applied in large quantity before SS and these reducing substances dissolved the soil Mn oxides and increased the amounts of available Mn, which led to the occurrence of Mn toxicity to the plants. 相似文献
7.
8.
《Communications in Soil Science and Plant Analysis》2012,43(4):331-339
Abstract A field experiment was conducted on an alkaline calcareous soil of Agronomy farm of the College of Agriculture, University of Udaipur to study the effect of sulphur on crop yield, leaf elemental composition, chlorophyll synthesis and activities of haematin enzymes. Application of elemental sulphur 21 days before planting with and without 0.1 per cent foliar sprays of H2SO4. increased crop yield by 137 to 197 per cent. Application of all these treatnents had no effect on nitrogen, phosphorus and potassium content of leaves but sulphur content was signficantly increased. Iron content of green leaves was decreased significantly 4 to 5 times together with an increase in the activity of catalase and peroxidase enzymes. Chlorosis in these soils appears to be on account of a reduced physiological availability of iron after absorption. 相似文献
9.
10.
烟草野火病菌(Pseudomonas syringae pv. tabaci)对烟草细胞内5种防御酶系统的影响 总被引:11,自引:0,他引:11
定期测定了感病品种红花大金元接种烟草野火病菌后叶片内5种酶活性的动态变化,研究结果表明:烟草接种病菌后,SOD活性先上升,后在8d下降,低于对照;POD活性接种后在1d略低于对照,后上升较快,10d达到高峰,此后一直高于对照;PPO活性在接种后1d低于对照15.8%,但此后上升,16d达到高峰,18d下降低于对照;CAT活性变化与POD相似,接种1d低于对照,但此后一直高于对照,并于6d达到高峰,10d虽有所下降,但接着升高;PAL活性与CAT、POD变化相似,接种后1d活性低于对照28.3%,其后上升,10d达到高峰,是对照的2.11倍,并维持较高的水平。 相似文献