早熟玉米杂交组合主要农艺性状与产量的灰色关联度分析

应用灰色关联度分析法,对19个玉米杂交组合性状进行分析.结果表明,各性状与产量的关联度大小依次为:株高>生育期>倒折率>穗行>行粒>秃尖>含水量>百粒重>穗位高>倒伏率>穗长>出籽率>穗粗.在育种工作中应该注重对株高、生育期和倒折率的选择,同时要结合不同性状间相互关系的综合表现进行育种,使玉米产量有较大提升.     

玉米苗枯病抗性鉴定方法的改进

采用玉米种子培养液对串珠镰刀菌分生孢子萌发抑制率和串珠镰刀菌毒素对玉米胚根、胚芽生长抑制率作为玉米苗枯病抗病鉴定指标,并与21个杂交玉米种苗枯病田间发病率进行相关分析来验证其准确性.结果表明,串珠镰刀菌毒素对玉米胚根、胚芽生长抑制率与玉米苗枯病田间发病率相关性较高,相关系数分别为0.925、0.919.该两种方法结果准确,可以作为玉米苗枯病抗性鉴定指标.     

不同浓度玉米生长调节剂对玉米氮代谢的影响

采用田间小区试验,研究了不同浓度新型玉米生长调节剂对玉米氮代谢的影响．结果表明,不同浓度玉米生长调节剂对灌浆期玉米功能叶、茎秆中氮素的转运,功能叶硝酸还原酶与蛋白水解酶活性,功能叶和籽粒的谷氨酰胺合成酶与转化酶活性均有明显的促进作用,显著增加玉米产量、籽粒蛋白质含量及籽粒蛋白质产量．在调节剂的3个浓度中,以PGRCl较合理．PGRCl处理玉米籽粒的蛋白氮含量比对照提高0．83g／kg,籽粒产量比对照增加13．18％,籽粒蛋白质产量比对照增加19．59％．     

山东地区夏玉米的高产种植技术

随着我国市场经济的不断发展和农业供给侧的不断改革,玉米价格呈现出大幅度下降趋势, 玉米种植面积也急剧下降。为了提高玉米的产量和效益,科学推广和应用玉米高产种植技术是当务 之急,同时也是玉米产业快速发展的必然趋势。基于以上情况,本文从精细播种、苗期管理、玉米穗期 管理、花粒期管理五个方面入手,研究了夏玉米高产种植技术。希望通过这次研究,为相关种植人员 提供有效的参考。     

Screening of antagonistic Trichoderma strains and their application for controlling stalk rot in maize

Maize is one of the major crops in China, but maize stalk rot occurs nationwide and has become one of the major challenges in maize production in China. In order to find an environment-friendly and feasible technology to control this disease, a Trichoderma-based biocontrol agent was selected. Forty-eight strains with various inhibition activities to Fusarium graminearum, and Fusarium verticillioides were tested. A group of Trichoderma strains(DLY31, SG3403, DLY1303 and GDFS1009) were found to provide an inhibition rate to pathogen growth in vitro of over 70%. These strains also prevented pathogen infection over 65% and promoted the maize seedling growth for the main root in vivo by over 50%. Due to its advantage in antifungal activity against pathogens and promotion activity to maize, Trichoderma asperellum GDSF1009 was selected as the most promising strain of the biocontrol agent in the Trichoderma spectrum. Pot experiments showed that the Trichoderma agent at 2–3 g/pot could achieve the best control of seedling stalk rot and promotion of maize seedling growth. In the field experiments, 8–10 g/hole was able to achieve over 65% control to stalk rot, and yield increased by 2–11%. In the case of natural morbidity, the control efficiency ranged from 27.23 to 48.84%, and the rate of yield increase reached 11.70%, with a dosage of Trichoderma granules at 75 kg ha~(-1). Based on these results, we concluded that the Trichoderma agent is a promising biocontrol approach to stalk rot in maize.     

不同发育阶段干旱胁迫对玉米株高、果穗性状及产量的影响

摘要：为研究同一品种不同发育阶段干旱胁迫对玉米植株高度、果穗性状以及产量的影响程度,设置全生育期供水充足、大田自然干旱、拔节期控水、抽雄期控水、拔节期控水-复水、抽雄期控水-复水6种处理的对比试验,用对比分析的方法比较不同处理下玉米的株高、果穗性状以及产量之间的差异。结果表明：干旱胁迫使玉米植株矮小,果穗长减小7.88%~54.55%,果穗粗减小14.58%~52.08%,秃尖长度增大66.67%~258.33%,百粒重减小0.99%~41.36%,单株产量减少15.03%~94.41%,最终导致减产23.57%~90.03%。不同发育阶段干旱胁迫对产量的影响差异显著,全生育期干旱最为严重,几乎无产量,拔节期次之,抽雄期干旱胁迫影响最小,复水后由于部分补偿了前期干旱胁迫所减少的生物量,果穗性状、百粒重均有好转,减产幅度减轻。     

覆膜方式对雨养饲用玉米生长、产量及水分利用效率的影响

为促进冀西北坝上高寒半干旱区旱地饲用玉米稳产、高产和提高水分利用效率,于2016—2017年在农业部张北农业资源与生态环境重点野外观测试验站,进行了露地平作(ck),双垄沟覆膜、微垄覆膜、土下覆膜处理对饲用玉米叶面积指数、干物质积累、土壤水分、产量和水分利用效率影响的试验研究。结果表明,在丰水年,双垄沟覆膜处理效果最好,与露地相比叶面积指数、干物质积累量、土壤蓄水量分别提高36.45%、70.24%、13.25%,产量和水分利用效率提高了67.24%和87.66%;在欠水年,土下覆膜处理效果最佳,较露地增产71.31%,水分利用效率提高81.69%。双垄沟覆膜是寒旱区提高饲用玉米产量和高效利用降水的有效技术,土下覆膜则更适用于土壤贮水丰富农田的高水效生产。     

Functional polymorphism among members of abscisic acid receptor family(ZmPYL) in maize

Pyrabactin resistance 1-like proteins(PYLs) are direct receptors of abscisic acid(ABA). For the redundant and polymorphic functions, some members of the PYL family interact with components of other signaling pathways. Here, 253 positive colonies from a maize cDNA library were screened as interacting proteins with the members of ZmPYL family. After sequencing and function annotation, 17 of 28 interaction combinations were verified by yeast two-hybrid(Y2 H). The germination potential, taproot length and proline content of a quartet mutant of Arabidopsis PYL genes were significantly deceased comparing to the wild type(WT) under alkaline stress(pH 8.5) and 100 μmol L–1 methyl jasmonate(MeJA) induction. The malondialdehyde(MDA) content was significantly increased. After germinating in darkness, the characteristics of dark morphogenesis of the quartet mutant seedlings were more obvious than those of the WT. The differential expression of the related genes of photomorphogenesis in the mutant was much more than that in the WT. Three light and two JA responsive cis-affecting elements were identified during the promoter sequences of the AtPYL1 and AtPYL2 genes. These results suggested that functional polymorphism has evolved among the members of ZmPYL family. In response to developmental and environmental stimuli, they not only function as direct ABA receptors but also interact with components of other signaling pathways mediated JA, brassinosteroid(BR), auxin, etc., and even directly regulate downstream stress-related proteins. These signaling pathways can interact at various crosstalk points and different levels of gene expression within a sophisticated network.     

Effect of three insect-resistant maizes expressing Cry1Ie,Cry1Ab/Cry2Aj and Cry1Ab on the growth and development of armyworm Mythimna separata(Walker)

Three transgenic maize events(IE09 S034, Shuangkang 12–5 and C0030.3.5) produced Cry1 Ie, Cry1 Ab/Cry2 Aj and G10-EPSPS, Cry1 Ab and EPSPS, respectively, all of which target the Asian corn borer. The oriental armyworm Mythimna separata(Walker) is the secondary target. In this study, the effects of the three Bt maizes on the development and survival of armyworm were studied. The results showed that IE09 S034 had insecticidal activity against 1 st instar larvae, and the survival rate of armyworm fed with Bt maize for 10 days was 46.2%, significantly lower than that of the control. The larvae at 3 rd–6 th instar were more tolerant of the Bt toxin than the early instar larvae. However, Shuangkang 12-5 had good insecticidal activity against 1 st–5 th instar larvae. The mortality was nearly 100% when the larvae were fed with Shuangkang 12-5 before 3 rd instar, and the toxin had quick-acting efficacy. This event significantly inhibited the development of armyworm; that is, the larval duration of the 3 rd and 4 th instar larvae fed with Shuangkang 12-5 was prolonged by 4.5 and 3.0 days, respectively. The pupal weight and egg number were also significantly lower than those of the control. For C0030.3.5, it could control 1 st–5 th instar larvae effectively. The mortality rates were all over 50% if 1 st–3 rd larvae were fed with this event. The pupal weight of 4 th–6 th instar larvae fed with Bt maize were only 53.9, 56.8 and 54.6%, respectively, compared to that of the control. The number of eggs laid was significantly less than the control. The results indicate that all three transgenic maize events exhibit the potential to provide effective control of early instar larvae of armyworm, which can be commercialized in future to control lepidoptera pests such as Asian corn borer and armyworm.     

Effects of post-silking water deficit on the leaf photosynthesis and senescence of waxy maize

Waxy maize is widely cultivated under rainfed conditions and frequently suffers water shortage during the late growth stage. In this study, a pot trial was conducted to examine the effects of post-silking drought on leaf photosynthesis and senescence and its influence on grain yield. Two waxy maize hybrids, Suyunuo 5(SYN5) and Yunuo 7(YN7), were grown under the control and drought(soil moisture content was 70–80% and 50–60%, respectively) conditions after silking in 2016 and 2017. The decrease in yield was 11.1 and 15.4% for YN7 and SYN5, respectively, owing to the decreased grain weight and number. Post-silking dry matter accumulation was reduced by 27.2% in YN7 and 26.3% in SYN5. The contribution rate of pre-silking photoassimilates transferred to grain yield was increased by 15.6% in YN7 and 10.2% in SYN5, respectively. Post-silking drought increased the malondialdehyde content, but decreased the contents of water, soluble protein, chlorophyll, and carotenoid in the leaves. The weakened activities of enzymes involved in photosynthesis(ribulose-1,5-bisphosphate carboxylase and phosphoenolpyruvate carboxylase) and antioxidant system(catalase, superoxide dismutase and peroxidase) reduced the photosynthetic rate(P_n) and accelerated leaf senescence. The correlation results indicated that reduced Pn and catalase activity and increased malondialdehyde content under drought conditions induced the decrease of post-silking photoassimilates deposition, ultimately resulted in the grain yield loss.