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101.
AIM: To investigate the effect of paricalcitol (P) on renal tubulointerstitial fibrosis and the underlying mechanisms in diabetic nephropathy (DN).METHODS: DN rat model was induced by a single intraperitoneal injection of streptozotocin after fasting. The animals were randomly divided into 2 groups:the DN rats in paricalcitol-intervened group (group P) were injected intraperitoneally with paricalcitol dissolved in propylene glycol after the day when the model was induced successfully at a dose of 0.4 μg/kg (3 times a week); the DN rats in DN group (group D) were given isopyknic propylene glycol. Normal control group (group C) was also set up. The samples of blood, urine and renal tissue were collected after intervention of paricalcitol for 12 weeks. The biochemical indexes were measured. The renal tissues were used for pathologic observation and determining the expression of transforming growth factor-β1 (TGF-β1), Wnt-4, β-catenin and Klotho by immunohistochemistry and Western blotting. In addition, the correlation among the above indexes was analyzed.RESULTS: (1) Scr, BUN and 24 h urine protein increased significantly in group D compared with group C, while decreased in group P compared with group D (P<0.05). (2) The area of renal tubulointerstitial fibrosis increased in group D compared with group C, while decreased in group P compared with group D (P<0.05). (3) The expression of Klotho decreased, while the expression of TGF-β1, Wnt-4 and β-catenin increased in group D compared with group C (P<0.05). Compared with group D, the expression of Klotho increased, while the expression of TGF-β1, Wnt-4 and β-catenin decreased in group P (P<0.05). (4) The expression of Klotho was negatively correlated with the fibrosis area, TGF-β1, Wnt-4 and β-catenin (P<0.05).CONCLUSION: Paricalcitol inhibits renal tubulointerstitial fibrosis in DN by promoting the expression of renal Klotho, and inhibiting Wnt/β-catenin signaling pathway activation and TGF-β1 synthesis. 相似文献
102.
为明确在覆膜滴灌条件下玉米品系(种)主要农艺性状与产量的关系,应用DPS软件对15个玉米杂交品系(种)的主要农艺性状和产量性状进行相关性、灰色关联度等分析,结果表明:籽粒重、穗重、产量的变异系数较大;玉米产量与生育期、籽粒水分含量、穗重、籽粒重呈显著正相关关系;聚类分析可将参试的15个品系(种)划分为3类,第Ⅲ类表现良好,值得进行推广种植。灰色关联度分析表明,玉米籽粒重、穗重、生育期、籽粒水分含量等性状是影响玉米产量的主导因素,在玉米高产育种中,应以其为主攻目标,提高玉米后代的有效选择,加速高产育种进程。 相似文献
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The assay was aimed to explore the biological characteristics of bone morphogenetic protein 4 (BMP4) of sheep,NCBI,DNAMAN DNAStar,TMHMM Server v.2.0,PsortⅡ,SignalP various bioinformatical softwares were used to speculate the physical and chemical properties,hydrophobic property,phosphorylation site,conservative structure domain,protein secondary structure of BMP4 protein.Also,the three-dimensional structure was forecasted with the SWISS-MODEL Workspace software.The results indicated that the BMP4 of sheep had high homologies with the BMP4 of various species.The encoded protein was a hydrophilic protein which was unstable.There was no transmembrane regions and it was likely to be located in the nucleus.What was more,there was signal peptide and eighteen phosphorylation sites.Through the forecast of functional domains,the protein had two functional domains,including the transforming growth factor-beta (TGF-beta) superfamily and TGF-beta propeptide superfamily.The result was consistent with the function of BMP4 gene family,it also demonstrated that BMP4 was a growth factor and it had the function of signal transduction.The amino acid homology between the predicted 3D structure of protein and template 3bmp.1.A was 88.29%.The bioinformatics analysis of BMP4 gene could provide reference for the further study in practice. 相似文献
106.
The present investigation was conducted to assess the ameliorative effects of foliar‐applied trehalose on growth, photosynthetic attributes, water relation parameters and oxidative defence mechanism in two maize cultivars under field water deficit conditions. Various components of the experiment comprised two maize cultivars (EV‐1098 and Agaiti‐2002), two water‐stress levels (irrigation after 2 weeks and irrigation after 3 weeks during the entire period of growth), and two levels of trehalose (0 and 30 mm ) and four replicates of each treatment. Water stress significantly reduced the plant biomass production, photosynthetic attributes and water relation parameters in both maize cultivars. In contrast, water stress considerably increased the leaf malondialdehyde (MDA) contents, the activities of antioxidant enzymes such as peroxidase (POD), superoxide dismutase (SOD) and catalase (CAT), and the levels of non‐enzymatic compounds such as ascorbic acid and tocopherols. In contrast, water stress caused a marked reduction in leaf phenolic contents. Foliar‐applied trehalose significantly increased plant biomass production, and improved some key photosynthetic attributes and plant–water relation parameters. The ameliorative effect of exogenously applied trehalose was also observed on the activities of some key antioxidant enzymes (POD and CAT) and non‐enzymatic compounds (tocopherols and phenolics). Overall, exogenously applied trehalose considerably improved drought tolerance of maize plants by up‐regulating photosynthetic and water relation attributes as well as antioxidant defence mechanism. 相似文献
107.
为了优化以鲜食甜糯玉米为主要原料制备甜糯玉米酒的原料配方。试验运用模糊数学原理,将甜糯玉米酒感官品质数学抽象量化,以量化后的感官品质为指标,通过混料试验设计,建立原料配方与感官品质之间的回归模型。结果表明:该模型的拟合程度好(R=0.984 4,P0.001),可用于预测和分析甜糯玉米酒的感官品质。经模型优化可得,感官品质评价为"好"的甜糯玉米酒原料配方为鲜食糯玉米(66%)、鲜食甜玉米(17%)和糯米(17%)。本研究酿造的甜糯玉米酒的酒精度仅为4.75%,远低于一般低度酒,符合现代人的饮酒需求。 相似文献
108.
Can additional N fertiliser ameliorate the elevated CO2‐induced depression in grain and tissue N concentrations of wheat on a high soil N background?
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M. Tausz R. M. Norton S. Tausz‐Posch M. Löw S. Seneweera G. O'Leary R. Armstrong G. J. Fitzgerald 《Journal of Agronomy and Crop Science》2017,203(6):574-583
Elevated CO2 stimulates crop yields but leads to lower tissue and grain nitrogen concentrations [N], raising concerns about grain quality in cereals. To test whether N fertiliser application above optimum growth requirements can alleviate the decline in tissue [N], wheat was grown in a Free Air CO2 Enrichment facility in a low‐rainfall cropping system on high soil N. Crops were grown with and without addition of 50–60 kg N/ha in 12 growing environments created by supplemental irrigation and two sowing dates over 3 years. Elevated CO2 increased yield and biomass (on average by 25%) and decreased biomass [N] (3%–9%) and grain [N] (5%). Nitrogen uptake was greater (20%) in crops grown under elevated CO2. Additional N supply had no effect on yield and biomass, confirming high soil N. Small increases in [N] with N addition were insufficient to offset declines in grain [N] under elevated CO2. Instead, N application increased the [N] in straw and decreased N harvest index. The results suggest that conventional addition of N does not mitigate grain [N] depression under elevated CO2, and lend support to hypotheses that link decreases in crop [N] with biochemical limitations rather than N supply. 相似文献
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为探讨苦瓜抗白粉病的生理生化机制,以对白粉病不同抗性的4个苦瓜品系为材料,研究苗期感染白粉病菌后,苦瓜叶片生理生化指标的变化。结果表明:接种后,叶绿素和可溶性蛋白质量分数均呈先上升后降低的趋势;可溶性糖质量分数呈下降-上升-下降-上升的趋势;抗病品系的可溶性糖、叶绿素质量分数和过氧化物酶(POD)、多酚氧化酶(PPO)活性均高于或显著高于感病品系和高感品系;抗病品系的抗坏血酸(AsA)质量分数的上升和下降的幅度均小于感病品系和高感品系;接种后10~20d,抗坏血酸过氧化物酶(APX)活性表现为抗病品系感病品系高感品系。叶绿素、可溶性糖质量分数和POD、PPO活性与病情指数呈显著或极显著负相关。综上说明,白粉病菌侵染苦瓜后,抗病品系可通过保持较高的叶绿素质量分数,增加可溶性糖、可溶性蛋白、AsA质量分数及增强POD、PPO、APX活性来提高抗病性。叶绿素、可溶性糖质量分数和POD、PPO活性均可作为苦瓜对白粉病抗性早期鉴定的指标。 相似文献