春小麦蛋白质、脂肪、淀粉含量间的相关与回归分析

本文通过对20个春小麦品种(或品系)蛋白质、脂肪、淀粉百分含量间的相关、回归、协方差分析,表明蛋白质百分含量与淀粉百分含量间为极显著的负相关;脂肪百分含量与淀粉百分含量间为极显著的负相关;蛋白质百分含量与脂肪百分含量间为极显著的正相关。并建立了淀粉、脂肪、蛋白质百分含量间的回归方程。在同一试验条件下,各品种(或品系)蛋白质、脂肪、淀粉百分含量变异系数的大小,在一定程度上反映出各品种在品质性状上的纯合程度。变异系数愈小,表明纯合程度愈高;变异系数愈大,表明纯合程度愈低。因此,在那些变异系数较大的品种(或品系)中,可选出表型性状与原品种(或品系)基本相似,而在品质性状上却有较大提高的优质新品系。     

Decreased transport of orally administered protein into the blood circulation of developing juveniles of Japanese eel Anguilla japonica

ABSTRACT: To clarify the quantitative changes in the transport of orally intubated protein into the blood circulation as macromolecules in development, immunoglobulin Y (IgY) extracted from chicken eggs was administered orally to juvenile Japanese eel, Anguilla japonica . For the first experiment, which was performed before the commencement of artificial feeding, the oral delivery of 2.0 μg/0.1 g bodyweight of IgY resulted in a rapid increase in plasma IgY to a maximum of 2.30 μg/mL. However, the transport of IgY into the blood decreased significantly in the experiments that followed, which were performed after 12, 25 and 42 days. During this period, bodyweight increased approximately by a factor of eight, and rapid growth of the stomach was observed histologically. Possible contributions for the development of the alimentary canal to the diminishment of intestinal protein assimilation are discussed.     

不同饲养水平下蛋白质与氨基酸在阉牛小肠内消化率的研究

选择6头体重500～600kg ,安装有瘤胃、十二指肠前端和回肠末端瘘管的阉牛 ,分3期饲喂以玉米、棉籽饼和氨化稻草为基础原料配合的3种不同饲养水平的日粮 ,测定十二指肠、回肠和粪便中的粗蛋白质和各种必需氨基酸流量 ,计算粗蛋白质、真蛋白、总必需氨基酸、总非必需氨基酸和各种必需氨基酸在小肠内的表观消化率。结果显示 :随着饲养水平的下降 ,粗蛋白质、真蛋白、总必需氨基酸、总非必需氨基酸和亮氨酸、蛋氨酸、色氨酸、缬氨酸、胱氨酸的小肠表观消化率逐渐降低(P<0.05)。在每种饲养水平下 ,真蛋白的小肠表观消化率均大于粗蛋白质 (P<0.05) ;各种必需氨基酸的小肠表观消化率均有差异 ,精氨酸和亮氨酸的小肠消化率通常比真蛋白高 ,色氨酸和苏氨酸的小肠消化率比真蛋白低 ,但各种必需氨基酸的小肠表观消化率并没有一致的大小顺序。与日粮相比 ,十二指肠中的必需氨基酸组成发生了较大的变化 ;与回肠流量相比 ,粪便中的必需氨基酸排泄量也发生了较大的变化。     

13～18周龄笼养蛋鸭适宜蛋白质和蛋氨酸水平的研究

用 32 4羽满 1 2周龄的金定鸭 ,通过饲养试验、代谢试验、屠宰试验和对试鸭产蛋期 (1 9～ 2 8周龄 )产蛋性能的观察 ,研究了在等能条件下(ME :1 1 51MJ/kg) ,不同蛋白质水平 (CP :1 3 %、 1 5 %、 1 7% )和蛋氨酸水平 (Met/CP :0 0 1 7、 0 0 2 2、 0 0 2 7)组成的 9种饲粮对 1 3～ 1 8周龄笼养蛋鸭的生长性能、养分利用、体成分沉积和产蛋期产蛋性能的影响。结果表明 :该阶段饲粮粗蛋白、蛋氨酸水平对体增重影响不显著 (P >0 0 5) ;体增重与蛋氨酸 (% )呈一定的正相关 (r=0 4574) ;CP为 1 3%、Met/CP为 0 0 2 7组采食量一直较稳定、较高 ,且饲料效率又好。代谢试验 :不同粗蛋白、蛋氨酸水平饲粮对试鸭氮存留率和能量的利用率影响显著(P <0 0 5或P<0 0 1 ) ,且它们随饲粮CP水平的升高而降低 ,随蛋氨酸水平升高而升高 ;磷的利用率也分别以CP为 1 3%的水平和Met/CP为 0 0 2 7的水平极显著地高于其他水平对磷利用率 (P <0 0 1 )。屠宰试验 :当Met/CP为 0 0 2 7时 ,体氮沉积量和体磷沉积量最佳。产蛋性能 :开产日龄和高峰日龄与 1 3～ 1 8周龄CP食入量呈弱的负相关 (r=- 0 1 83 3和r=- 0 0 984) ,与蛋氨酸食入量呈正相关 (r =0 5780和r =0 435 0 )。饲粮粗蛋白、蛋氨酸水平对 1 9～ 2 8周龄     

柠檬酸废液深层培养双孢蘑菇菌丝蛋白的研究

柠檬酸工业废液中含有一定有效物质,如糖类、蛋白质、矿物质等,可供微生物利用。本文介绍了利用废液深层培养双孢蘑菇菌丝蛋白的可能性及条件。并用1吨发酵堆进行生产试验,探讨了生产工艺条件,分析了发酵液中底物、产物的动态变化。双孢蘑菇菌丝蛋白培养温度25℃,底物 pH6,发酵周期6天,菌球直径1～3mm,菌球数量4.8×10~3个/ml,菌丝温重达底物量的29.3%。在上述条件下,发酵液中菌球直经、菌丝数量、发酵液粘度受生物剪切作用的影响,粘度大,剪切作用大,菌球直径小,菌球数量则多。本文为新型单细胞蛋白的开发,促进物质的多层次利用,消除环境污染,提供了一个新途径。     

Impact of pig slurry on soil properties, water salinization, nitrate leaching and crop yield in a four-year experiment in Central Spain

Abstract. The repeated application of pig slurry to agricultural soils may result in an accumulation of salts and a risk of aquifer pollution due to nitrate leaching and salinization. Under Mediterranean conditions, a field experiment on a sandy loam soil (Typic Xerofluvent) was performed with maize (Zea mays) in 1998, 1999 and 2001 to study the effects of applying optimal (P1) and excessive rates (P3) of pig slurry on soil salinization, nitrate leaching and groundwater pollution. The rate of pig slurry was established considering the optimal N rate for maize in this soil (170, 162 and 176 kg N ha^?1 for 1998, 1999 and 2001, respectively). Pig slurry treatments were compared to an optimal N rate supplied as urea (U) and a control treatment without N fertilizer (P0). The composition of the slurries showed great variability between years. Mean NO₃^? leaching losses from 1998 to 2001 were 329, 215, 173 and 78 kg N ha^?1 for P3, P1, U and P0 treatments, respectively. The amount of total dissolved salts (TDS) added to the soil in slurry application between 1998 and 2001 was 2019 kg TDS ha^?1 for the P1 treatment and 6058 kg TDS ha^?1 for the P3 treatment. As a consequence, the electrical conductivity (EC) of the slurry‐treated soils was greater than that of the control soil. The EC correlated significantly with the sodium concentration of the soil solution. Over the entire experimental period, 2653, 2202 and 2110 kg Na ha^?1 entered the aquifer from the P3, P1 and P0 treatments, respectively. The P3 treatment did not significantly increase grain production in 1999 and 2001 compared with that achieved with the optimal N rate treatment (P1). This behaviour shows the importance of establishing application guidelines for pig slurry that will reduce the risk of soil and groundwater pollution.     

Effect of Nitrogen Rate and Stubble Height on Dry Matter Yield,Crude Protein Content and Crude Protein Yield of a Sorghum–Sudangrass Hybrid[Sorghum bicolor (L.) Moench × Sorghum sudanense (Piper) Stapf.] in the Three‐Cutting System

In this study, the effects of nitrogen (N) rate (60, 120, 180 and 240 kg N ha^?1 applied in three equal dressings at seeding and after the first and second cuttings) and stubble height (7, 14 and 21 cm) on the dry matter (DM) yield, crude protein (CP) content, and CP yield of a sorghum–sudangrass hybrid [Sorghum bicolor (L.) Moench × Sorghum sudanense (Piper) Stapf., cv. Pioneer 988] in the three‐cut system was investigated. The N rate had no significant effect in the first and third cuttings, but in the second cutting DM yields increased significantly with increase in N rate. The highest yield of 9.1 t ha^?1 was obtained with 80 kg N ha^?1 for the average of 2 years at the second cutting, but no significant difference was found among the 40, 60 and 80 kg N ha^?1 rates. CP content and yield were not significantly affected by N rate at the first and third cuttings, but CP content and yield were significantly affected by application of N at the second cutting. Stubble height had a significant effect on CP content at the third cutting. However, it had no significant effect on CP content at the first and second cuttings. Stubble height had a significant effect on the CP yield at the first cutting, but no significant effect on CP yield at the second and third cuttings.     

猪肺炎支原体膜蛋白的电泳分析及免疫印迹检测

以猪肺炎支原体Z株为试验材料,采用SDS-PAGE和Western印迹对该菌膜蛋白进行了分析。研究表明:猪肺炎支原体经A_(26)液体培养基培养,15000 r/min离心收集菌体细胞,低渗超声法破膜获得膜制剂后,应用12.5％的凝胶对膜蛋白进行SDS-PAGE分离,在电泳图谱上呈现出36条蛋白带,相对分子质量范围为1.18×10~4～9.12×10~4。同时以膜制剂作为抗原,分4次免疫试验兔,心脏采血,提取抗血清,并分离纯化得到IgG,然后进行Western印迹法检测。其结果发现在SDS-PAGE分离得到的36种膜蛋白(或多肽)中,有6种蛋白具有免疫原性。     

Quantitative capillary reversed passive latex agglutination test for C-reactive protein (CRP) in the dog

A capillary reversed passive latex agglutination test (capillary RPLA) was developed which allows quantification of serum C-reactive protein (CRP) within approximately 15 min. The logarithmic regression line (calibration curve) obtained after measuring each CRP concentration three times in twofold dilutions of a standard canine serum containing 222 g/ml of CRP was y=6.394+0.030x (r=0.995). Capillary RPLA permitted quantification of CRP in the range 6.9–222 g/ml. The coefficients of variation ranged from 10.28% to 12.40%. The recovery rates (percentage recovery) of CRP by capillary RPLA were within the range 87% to 106%. On measuring the CRP concentrations in sera from 78 dogs by capillary RPLA, single radial immunodiffusion (SRID) and enzyme-linked immunosorbent assay (ELISA), close correlations were demonstrated between SRID and capillary RPLA (y=7.250+1.109x, r=0.978), between SRID and ELISA (y=3.042+1.059x, r=0.967), and between capillary RPLA and ELISA (y=1.778+0.929x, r=0.962). Capillary RPLA may be considered useful as a routine biochemical technique for measurement of serum CRP concentration in the dog.Abbreviations CRP C-reactive protein - ELISA enzyme-linked immunosorbent assay - RPLA reversed passive latex agglutination test - SRID single radial immunodiffusion     

The rates of maize growth and lignin biosynthesis change after root-applied chalcone

The effects of root-applied chalcone at 0.15 mmol L⁻¹ on the growth and lignin biosynthesis in maize were investigated. The contents of 4-coumarate: CoA ligase (4CL, EC 6.2.1.12) substrates in maize shoots were increased more rapidly in the samples with chalcone application than in the control and the increase occurred at ≤ 3 h after the application (HAA). The lignin content was reduced by chalcone at ≤ 6 HAA. The shoot growth was suppressed by chalcone at ≤ 9 HAA. Consequently, the results suggest that chalcone suppressed maize growth by inhibiting monolignol biosynthesis.