不同温度下TMV侵染枯斑三生烟的LncRNA差异表达

【目的】 筛选不同温度下烟草花叶病毒（Tobacco mosaic virus,TMV）侵染后枯斑三生烟（Nicotiana tabacum var. Samsun NN）差异表达的长链非编码RNA（long non-coding RNA,lncRNA）,研究lncRNA在枯斑三生烟抗性反应中的作用。【方法】 N基因的温度敏感性使枯斑三生烟在25℃时具备对TMV的抗性、在31℃抗性丧失,在这两个温度条件下对枯斑三生烟接种TMV和磷酸盐缓冲盐水（phosphate buffered saline,PBS）,48 h后提取系统叶总RNA,构建链特异性文库后进行深度测序。对测序结果进行过滤后利用HTSeq将有效数据与近缘品种TN90（N. tabacum var. TN90）基因组比对,筛选得到lncRNA后利用FPKM法估计lncRNA的表达水平。通过edgeR筛选差异表达lncRNA（differentially expressed lncRNA,DElncRNA）,并利用qRT-PCR技术对这一结果进行验证。通过共定位及共表达分析预测DElncRNA的靶基因,通过参考基因组注释、GO和KEGG富集分析研究靶基因的功能。【结果】 4个处理共12个样本经lncRNA-seq各测得约8 000万条clean reads,共获得4 737条已知lncRNA、40 169条新lncRNA。其中64个lncRNA在不同温度条件下TMV侵染后存在差异表达,qRT-PCR测定结果显示这些lncRNA的测序正确率在80%左右,表明本研究所得测序数据具备较高的可信度。对DElncRNA进行靶基因预测,发现一些基因同时被25℃下调和31℃上调的DElncRNA靶向。靶基因注释功能丰富,主要参与植物抗病、激素和代谢等生理过程。部分可能与激素通路相关的lncRNA,在25℃下TMV侵染时呈现下调趋势,而在31℃下TMV侵染则呈现上调趋势。GO富集分析显示靶基因主要参与构成膜、囊泡等组分,具备钙、钾离子通道抑制剂活性等分子功能,使相应离子得以转运引发随后的反应,同时也参与发病、抗原加工和呈现、细胞分裂素代谢等生理过程。KEGG分析发现靶基因显著富集在植物激素信号转导通路,25℃下调和31℃上调的DElncRNA靶基因同时富集在激素信号传导、ABC运输蛋白、苯丙烷类生物合成等通路。【结论】 不同温度（25℃和31℃）条件下TMV侵染枯斑三生烟后,长链非编码RNA差异表达,DElncRNA通过作用于激素信号传导、物质转运等过程参与寄主系统获得性抗性反应。研究结果可为揭示植物系统获得性抗性中lncRNA的调控功能以及新型抗病毒技术开发提供依据。     

遵义县白菜不同配方施肥效果初探

为指导农户生产,进行了6个不同施肥量比较试验.结果表明：白菜施肥量为清粪水1500kg/667m2,尿素20kg/667m2,普钙50kg/667m2,氯化钾13kg/667m2,单产高,经济效益好,可在遵义县推广.     

施肥对猕猴桃鲜果产量及品质的影响

试验比较5个不同施肥水平对坡地猕猴桃果园鲜果产量及其内在品质的影响.结果表明,随着施肥水平的提高,猕猴桃鲜果的产量也明显提高,配方施肥的增产幅度更为明显.在低施肥水平的条件下,增加施肥对提高猕猴桃鲜果品质有正面的作用;高施肥水平对鲜果品质在总体上没有不良的影响.同时,对施用有机肥和配方施肥的作用进行讨论.     

新型肥料与沼液替代在农业生产中的应用

随着现代农业的发展和农业生产技术的进步，通过探索新型肥料研发与推广、种养循环有机替代、农业生产水肥耦合运筹等新技术新模式，在保障作物稳产增产前提下,实现了减少农业生产投入、降低生产成本、提高生态效益的目标。本文介绍了新型肥料的研发推广和沼液替代化肥研究进展，归纳了新型肥料与沼液替代化肥技术效果，为优化施肥技术的应用提供参考。     

森林类型对大气中PM2.5值的影响

从2014年9月开始到2015年8月,在长沙市郊湖南省林业科学院杜家冲试验林场选择不同森林类型林分,对其大气中PM2.5持续监测1年。结果表明:该区大气中PM2.5值最高的为阔叶混交林,其次为2个空旷地,最低的为樟树林;大气中PM2.5值在不同林分间差异不显著,在不同时间段间差异显著。     

施肥方式对不同密度下玉米光合生理的影响

为进一步研究玉米品种登海605不同密度和施肥方式对光合生理的影响，挖掘其增产潜力，建立高产栽培模式。本试验设计5个密度处理（D1、D2、D3、D4、D5）、3种施肥方式处理（S1：农大缓释肥、S2：分次施肥、S3：一次性施肥），并对不同处理的光合指标、叶面积指数、光合有效辐射、透光率等指标进行测定，结果表明：不同的施肥方式上层、中层、下层光合指标变化规律表现一致，各层次表现为上层>中层>下层，S2>S1>S3，玉米植株上层无论哪种处理都能获得足够的光能和CO2，光合指标相差不大，适宜的密度和施肥方式将获得更多的光能、有效辐射，合成有机物，形成更多的干物质积累，从而提高产量。     

机械化深施肥技术及推广

机械化深施肥技术是作物施肥作业的技术创新,可提高作业效率、肥料利用率和作物产量.但机械化深施肥技术要达到普及推广,还须采取多种措施:加大推广示范和政策扶持力度,加强深施肥技术的农机与农艺配套研究,研究制定机械施肥作业技术标准;农机制造企业要提高产品性能和质量,提高设备使用的可靠性;同时,化肥生产企业也应参与研究,共同开发与深施技术配套的肥料.     

Design and Experiment of Slave Computer Control System for Applying Variable-rate Liquid Fertilizer

In order to increase the applying rate of liquid fertilizer and reduce environmental pollution, a slave computer control system for applying variable-rate liquid fertilizer was designed. The system used SMC as core processor and electrically controlled pressure regulator as execution component. The characteristic equation of the system was obtained by using classical control theory. Results indicated that the characteristic equation met the requirements of routh-criterion, which indicated the working process of the system was stable. Performance of the slave computer was verified via bench tests. Results demonstrated that there was no significant influence on the response from interclass error. The fertilization error was less than 0.9, and the fertilization accuracy was larger than 97%. The liquid fertilizer emitted by the fertilizing devices had no significant difference in uniformity, which met the demands of the slave computer control system for applying variable-rate liquid fertilizer.     

不同合成树脂工艺对改性材性能影响研究

为研究树脂对改性材性能的影响，采用2种不同工艺合成三聚氰胺-脲醛树脂（MUF），测试了树脂的相关性能。结果表明，不同合成工艺路线下制备的 MUF 树脂在固体含量、粘度、固化时间、游离甲醛含量间存在显著差异。最终树脂的分子结构类型相似性极高，但相同结构组分在不同树脂中所占比例各有差异。羟甲基基团在MUF2中所占比例高，而亚甲基桥键及醚键在MUF1中含量高。MUF1改性材的增重率（weight percent gain，WPG）值更大，但MUF2改性材的抗溶胀性（anti-swelling efficiency，ASE）和体积膨胀率（bulking rates，B）更高，MUF2改性材的尺寸稳定性更好。     

Response of the soil fungal community to multi-factor environmental changes in a temperate forest

Both environmental and climatic changes are known to influence soil microbial biomes in terrestrial ecosystems. However, there are limited data defining the interactive effects of multi-factor environmental disturbances, including N-deposition, precipitation, and air temperature, on soil fungal communities in temperate forests. A 3-year outdoor pot experiment was conducted to examine the temporal shifts of soil fungal communities in a temperate forest following N-addition, precipitation and air temperature changes. The shifts in the structure and composition of soil fungal communities were characterized by denaturing gradient gel electrophoresis and DNA sequencing. N-addition regimen induced significant alterations in the composition of soil fungal communities, and this effect was different at both higher and lower altitudes. The response of the soil fungal community to N-addition was much stronger in precipitation-reduced soils compared to soils experiencing enhanced precipitation. The combined treatment of N-addition and reduced precipitation caused more pronounced changes in the lower altitude versus those in the higher one. Certain fungal species in the subphylum Pezizomycotina and Saccharomycotina distinctively responded to N fertilization and soil water control at both altitudes. Redundancy discrimination analysis showed that changes in environmental factors and soil physicochemical properties explained 43.7% of the total variability in the soil fungal community at this forest ecosystem. Variations in the soil fungal community were significantly related to the altitude, soil temperature, total soil N content (TN) and pH value (P < 0.05). We present evidence for the interactive effects of N-addition, water manipulation and air temperature to reshape soil fungal communities in the temperate forest. Our data could provide new insights into predicting the response of soil micro-ecosystem to climatic changes.