AREG对绵羊卵丘细胞葡萄糖代谢的影响

为研究双调蛋白（AREG）对绵羊卵丘细胞（Cumulus cells,CCs）葡萄糖代谢的影响,采集来源于中腔卵泡和小腔卵泡的卵丘-卵母细胞复合体（COCs）,利用荧光定量PCR检测中、小腔卵泡卵丘细胞葡萄糖代谢相关基因表达;添加AREG进行卵丘细胞的体外培养和卵母细胞的体外成熟（IVM）,检测卵丘细胞的增殖,测定培养液或细胞中的葡萄糖、乳酸、NADPH和ATP含量。结果表明：1）来源于小腔卵泡的卵丘细胞G6PDH、PFKL和PFKM的mRNA表达量以及细胞增殖能力显著低于中腔卵泡卵丘细胞（P<0.05）;2）较低浓度（10ng/mL）的AREG显著提高了中腔卵泡卵丘细胞的增殖能力（P<0.05）而对小腔卵泡卵丘细胞无影响（P>0.05）,在生长分化因子9(GDF9)和骨形态发生蛋白15(BMP15)的协同作用下,10ng/mL AREG可以显著提高两者的增殖能力（P<0.05）且两者间差异不显著（P>0.05）;3）在GDF9和BMP15的协同作用下,AREG可显著提高不同直径腔卵泡卵丘细胞培养液中的葡萄糖消耗、乳酸生成、NADPH生成以及细胞中的ATP...     

日粮添加蛋氨酸对幼兔生长发育、免疫性能及血液生化指标的影响

选择100只断奶新西兰兔,随机分成5组,在日粮中分别添加不同水平的蛋氨酸（0、0．2％、0．4％、0．6％、0．8％）,研究其对生长性能、氮代谢、免疫性能及血液生化指标的影响。结果表明：日粮添加蛋氨酸能显著提高日增重和饲料转化率（P〈0．05）,添加蛋氨酸0．4％时,日增重和料重比达最佳水平．分别为30．8g、3．29：1;蛋氨酸水平对氮代谢的影响差异不显著（P〉0．05）;蛋氨酸对胸腺指数的影响差异显著（P〈0．05）,当添加量为0．4％时,胸腺指数达到最大,但是对肝脏指数和脾脏指数无显著影响（P〉0．05）。对免疫球蛋白A、G、M含量也无显著影响（P〉0．05）;蛋氨酸对血清中总蛋白、白蛋白和血清尿素氮的含量无显著影响（P〉0．05）．     

抗牛布鲁氏菌独特型抗体杂交瘤细胞（Ｆ９）的生长和代谢

用RPMI1640培养基对抗牛布鲁氏菌独特型抗体杂交瘤细胞株(F9)进行培养，并对其生长代谢进行测定。该细胞不论接种1.1×10     

家禽脂类代谢调控机理的研究进展

利用营养和分子生物学手段来调控家禽脂类代谢 ,有赖于对其机理的深入了解。本文详细讨论了脂蛋白转运体系在家禽脂类代谢中的作用。通过营养因素和激素水平调控脂蛋白的合成、组装、转运和降解可影响机体脂肪沉积及蛋黄的形成。低密度脂蛋白LDL -受体基因家族在家禽脂蛋白代谢的调控过程中发挥着至关重要的作用     

生物类黄酮的生理功能及其应用研究进展

本文综述了类黄酮对动物脂质代谢与抗氧化作用的调节及其与动物内分泌、机体免疫的关系 ,并介绍了类黄酮在动物生产上的应用     

过氧化物酶体增殖剂受体与脂质代谢调控

综述了过氧化物酶体增殖剂受体在动物体内的分布、结构与转录调节机制及其对脂肪细胞分化、脂质代谢的调控。     

胆碱降低动物脂肪沉积的营养作用机理

胆碱是动物生长发育所必需的维生素。饲料中添加胆碱可显著降低动物脂肪沉积。基于胆碱代谢和蛋氨酸代谢,本文从胆碱氧化、蛋氨酸再甲基化、脂肪酸氧化三者之间的联系探讨了胆碱降低动物脂肪沉积的营养作用机理。     

Energy metabolism and apoptotic effect of microwave radiation on rat myocardial cells

AIM: To investigate the effect of microwave radiation at different intensities on the rat myocardium and its possible mechanism.METHODS: The rats were radiated by the intensity of 500, 1 000, 1 500 and 2 000 W/m² with 2 450 MHz microwave for 6 min. The heart tissue was collected 6 h after microwave radiation. ATP and mitochondria complex Ⅳ and Ⅴ were measured. The changes of the tissue structures were observed under transmission electron microscope. The apoptosis of the myocardial cells was detected by a cell analyzer. The protein level of cleaved caspase-3 was determined by Western blotting.RESULTS: The concentration of ATP and activity of mitochondria complex Ⅳand Ⅴ signi-ficantly decreased compared with control group in the cardiac tissues. The decreased number, morphological abnormalities such as dissolved cavitation, matrix and obvious tumefaction of mitochondria were observed under transmission electron microscope. The microwave radiation induced the apoptosis of myocardial cells in the rats. The cell apoptotic rate and the protein level of cleaved caspase-3 increased with increasing intensity of microwave radiation(P<0.05).CONCLUSION: Microwave radiation has obvious injury effect on the rat heart, which can cause cardiac energy metabolism dysregulation and cardiac myocyte apoptosis.     

Effect of Lysine Supplement on the Digestion and Metabolism of the Rumen in Small-tail Han Sheep

5 male Small-tail Han sheep with the body weight of 45 kg at 1.5 years old,fitted with permanent rumen fistula,to study of the effect of the supplement of the untreated commercial lysine on the rumen microbiocoenosis,rumen metabolism and whole body metabolism by 5×5 Latin square design (5 groups:control,control with low urea (equal nitrogen),low lysine,control with high urea (equal nitrogen),high lysine).The amounts of lysine-HCl supplement were 0,4.0 and 8.0 g/kg diet (as DM basis) in each group respectively,with two urea supplement control groups of equal amount of nitrogen.The results showed that by supplement of 4.0 and 8.0 g/kg of lysine-HCl diet (as DM basis) respectively,the voluntary intake of dry matter of sheep were increased by 5.5%(P>0.05) and 11.8%(P<0.05) respectively.The total amount of rumen bacteria were increased by 18.9%(P<0.01) and 23.9%(P<0.01) respectively,as the amount of rumen Coccus was increased by 21.2%(P<0.01) and 30.1%(P<0.01) respectively.The amount of rumen big Bacillus was decreased by 16.7%(P<0.05) and 33.3%(P<0.01) respectively.The total VFA of was increased by 9.6%(P<0.05) and 12.2%(P<0.01) respectively,as the butyric acid was increased by 12.8%(P>0.05) and 20.2%(P<0.01) respectively.The apparent digestibility of organic matter was increased by 6.4%(P>0.05) and 10.0%(P<0.05) respectively,the apparent digestibility of crude protein was increased by 7.0%(P>0.05) and 18.3%(P<0.01) respectively;And nitrogen retention was increased by 46.4%(P>0.05) and 110.7%(P<0.01) respectively.However,there was no effect of lysine supplement on the amounts of the rumen protozoa and fungi,and the concentration of rumen ammonia nitrogen.It was concluded that the supplement of untreated commercial lysine to adult sheep would increase the voluntary intake,digestibility,nitrogen retention the rumen microbiocoenosis and the total amount of rumen bacteria,especially the rumen Coccus of adult sheep.The effect of lysine supplement could not be replaced by urea supplement completely.