新城疫快速诊断试纸条的研制及初步应用

以红色胶体金标记鸡新城疫(ND)抗体，兔抗鸡ND抗体和兔抗鸡二抗包被于硝酸纤维素膜上，研制出快速诊断鸡新城疫的免疫层析测试条。将试纸条插入样品中，利用滤膜的层析作用，使加在膜一端的样品，向另一端移动，在移动过程中抗原与抗体发生反应，产生肉眼可见的红色条带。该法具有操作简便、快速准确、特异性强、重复性好，无需任何仪器设备等特点，对于鸡场中快速诊断及防治本病具有重大意义。     

俄罗斯沙棘在砂金过采区植被恢复中的应用试验

黑河地区砂金过采区水土流失、污染严重,利用俄罗斯沙棘进行植被恢复,有针对性地提出了当地治理环境、种植俄罗斯沙棘技术,可在同类地区试验推广.     

Establishment and Application of Colloidal Gold Immune Chromatography Test Method for Detection of Canine Parvovirus Hemagglutination Inhibition Antibody Titer

The study was aimed to use colloidal gold immune chromatography technology to establish a rapid method for detection of canine serum canine parvovirus (CPV) hemagglutination inhibition (HI) titer and CPV vaccine immunization effect assessment.Double antibody sandwich method and monoclonal antibodies of anti-CPV hemagglutination antigen were used to prepare CPV antigen test strip.Canine serum with different proportion respectively was mixed with quantitative CPV antigen for full reaction,then dropped the mixture into the CPV colloidal gold test strip,so according to the highest serum dilution ratios when the test strip line T (line T) vanishes,it was to judge CPV antibodies in serum of the HI titer.This method had been used to detect 86 canine serum samples,at the same time,analyzing and comparing it with traditional hemagglutination inhibition test method.The results showed that the CPV antigen detection test strip was successfully prepared,and the reaction conditions and results of the test strip for detecting the titer of CPV-HI in canine serum were determined.The results indicated that when detecting CPV antigen after the dilution of different ratios of canine serum,the highest serum dilution ratios when the strip line T vanished and the HI titer had positive correlation.The highest dilution ratios of canine serum multiplied by 4 was the HI titer.The results of two methods had 90.7% consistency.This experiment established the colloidal gold immune chromatography test strip for the detection of CPV-HI titers method initially.This CPV-HI detection provided a simple and fast test method for the effect evaluation of CPV vaccine immune.     

泥沙沉积与围垦对洞庭湖生态系统服务功能的影响

研究泥沙沉积和围垦对洞庭湖生态系统部分服务功能的影响结果表明,泥沙、围垦、湿地三者既互为条件,又互为因果关系,这种因果循环关系在湖泊生态系统中产生了泥沙淤积湿地浮涨钉螺孽生、害鼠繁殖、土地沙化,泥沙沉积湿地围垦生物资源量减少,泥沙沉积加围垦调蓄功能减弱洪涝频繁、水体污染、航道阻塞等一系列连锁反应,且很大程度上削弱或损害了湖泊服务功能,进而降低了部分服务功能的价值,即水产和饲草资源损失价值32 .1亿元,蓄水与供水功能损失价值1.5 3亿元,调蓄洪水与生物多样性损失价值4 1.92亿元,生态环境与航运功能损失价值74 .76亿元     

金属阳离子夹心三核金咪唑基化合物的电子结构和光物理性质

采用混合密度泛函方法,对一系列包含金属离子(Cr2+、W2+、Fe2+、Ru2+、Cd2+、Cu+、Ag+、Au+、In+、Tl+、Li+、Na+)的咪唑基三核金夹心化合物基态分子结构进行了优化;并采用含时密度泛函方法对这些夹心化合物的垂直激发态进行研究,描述了与发光过程相关的前线分子轨道特征.计算结果表明:夹心金属离...     

农村合作金融的国际比较分析以及对中国的启示

农村合作金融是农村金融系统的重要组成部分，可以成为农村经济发展的重要助推器。尽管在发达国家，商业银行的发展已经非常的成熟，但合作金融组织在这些国家尤其是农村仍然有其存在的必要性和合理性。本文以美国、德国、法国和日本作为研究对象，采用规范研究和实证研究相结合，理论分析与实际案例相结合的研究方法，综合分析这些发达国家农村合作金融组织的组织架构和运作特点，进行由点到面、由局部到全局的逻辑推理，最终提出中国农村信用社的改革路径，即“官退民进”；多元化经营战略；建立存款保险制度和完善相关法律法规等。     

华东某铀矿区稻米中Cr元素含量及其空间分布特征研究

利用等离子体质谱仪测定了某铀矿区周边稻米中重金属元素Cr的含量,重点对铀矿区稻米中的Cr元素含量及其空间分布特征进行了研究.结果表明,铀矿区内67.80％的稻米样品Cr含量超过国家标准（GB 2762-2005）;已经对环境造成了危害.铀矿区内稻米中Cr含量变化范围是0.37 ～ 13.20 mg/kg,平均含量为2.08 mg/kg.道路沿线区域、尾矿堆积区、水冶厂和矿床开采区下游及周边区域内稻米中Cr元素含量明显高于上游地区或远离上述区域的其他地区.矿床开采产生的废水与废渣、水冶厂的废液渗漏、尾矿露天堆积以及矿石运输过程中的矿渣洒落等原因可能是导致Cr元素随地表径流发生迁移进入稻田,从而使矿区稻米中Cr的含量较高的重要因素.     

Temporal (1958–1993) and spatial patterns of land use changes in Haut-Saint-Laurent (Quebec, Canada) and their relation to landscape physical attributes

In the last few years, landscape researchers have sought to understand temporal and spatial patterns of landscape changes in order to develop comprehensive models of land cover dynamics. To do so, most studies have used similar methods to quantify structural patterns, usually by comparing various landscape structural indices through time. Whereas the necessity for complementary approaches which might provide insights into landscape dynamics at some finer scale relevant to local managers has been expressed, few studies have proposed alternative methodologies. Moreover, the important relationship between the physical constraints of the landscape and land use dynamics has been seldom emphasized. Here we propose a methodological outline which was applied to the study of a rural landscape of Southern Quebec, Canada, to detect spatial and temporal (1958 to 1993) patterns of land cover changes at field, patch and landscape level. We then relate these patterns to the underlying physical structure of landscape elements using GIS and canonical correspondence analyses. We use the different geomorphological deposit types as stable discriminant factors which may constrain land use.Canonical correspondence analyses showed relations of land use and land use changes to the physical attributes of the landscape elements, whereas spatial analyses revealed very dynamic patterns at finer spatial and temporal scales. They highlighted the fact that not only the physical attributes of the landscape elements but also their spatial configuration were important determinants of land use dynamics in this area. Thus more land use changes occurred at the boundary between geomorphological deposit types than in any other locations. This trend is apparent for specific small-size changes (e.g. forest to crop), but not for the large-size ones (e.g. abandoned land to forest). Although land use changes are triggered by socioeconomic forces in this area, these changes are nevertheless constrained by the underlying physical landscape structure. A thorough comprehension of historical changes will enhance our capability to predict future landscape dynamics and devise more effective landscape management strategies.     

表面活性剂TX-10 对溶液表面张力及水稻植株持液量的影响

以生物染料丽春红-G作指示剂,采用称重法和比色法测定了水稻叶片持液量和丽春红-G的回收率。结果表明:水稻叶片对清水的持留量为0.05 mg/cm2,表面活性剂TX-10能降低清水的表面张力,增加其在水稻叶片上的持留量;与用清水配制的丽春红-G溶液喷洒,丽春红-G在水稻植株上的沉积率(29.83%)相比,用TX-10 60mg/L溶液配制的丽春红-G溶液喷洒,丽春红-G在植株上的沉积率显著提高(120.32%)。     

基于纳米金合成法评估玉米幼苗耐旱性

为检测干旱胁迫前后玉米幼苗的抗氧化活性进而评估玉米幼苗耐旱性,选取‘郑单958’、‘浚单20’、‘登海662’和‘豫玉22’4个玉米品种,采用纳米金合成法对其抗氧化活性进行检测。以透射电镜TEM、X射线光电子能谱XPS表征纳米金,进行条件优化和方法学确证。同时与DPPH法、FRAP法和FC法3种检测抗氧化活性方法的结果进行对比。采用表型鉴定、SOD酶活性和MDA含量测定的方法对玉米幼苗的耐旱性进行检测,并对上述方法检测结果进行相关性分析与主成分分析。结果表明,纳米金合成法检测干旱胁迫前后4个品种玉米幼苗抗氧化活性由强到弱为‘郑单958’、‘浚单20’、‘登海662’、‘豫玉22’,与传统方法和耐旱性检测结果具有一致性。相关性分析和主成分分析结果显示,纳米金合成法与其他方法相关性良好,且在解释总变异中占有较高的表达量,表明用纳米金合成法评估玉米幼苗耐旱性具有良好可行性。