Selecting the hologenome to breed for an improved feed efficiency in pigs—A novel selection index

Most traits in animal breeding, including feed efficiency traits in pigs, are affected by many genes with small effect and have a moderately high heritability between 0.1 and 0.5, which enables efficient selection. Since the microbiota composition in the gastrointestinal tract is also partly heritable and was shown to have a substantial effect on feed efficiency, the host genes affect the phenotype not only directly by altering metabolic pathways, but also indirectly by changing the microbiota composition. The effect of the microbiota composition on the breeding value of an animal is the conditional expectation of its breeding value, given the vector with microbiota frequencies, that is The breeding value of an animal can therefore be decomposed into a heritable contribution that arises from an altered microbiota composition and a heritable contribution that arises from altered metabolic pathways within the animal, so Instead of selecting for breeding value , an index comprising the two components and with appropriate weights, that is , can be used. The present study shows how this breeding strategy can be applied in pig genomic selection breeding scheme for two feed efficiency traits and daily gain.     

环境管制政策的实施效果评价——以九龙江流域"限猪令"为例

环境管制一直是我国环境污染治理所采取的主要政策之一,对我国的环境保护起到了一定的积极作用,同时也存在一些弊端,致使其成效不甚显著甚至背离初始目标.在此情况下,如何评价环境管制政策绩效具有重要的理论和实际意义.本文以九龙江流域"限猪令"政策为例,选取政策目标实现度、主要环保指标实现、环保投入效益、公众参与及满意度四个方面...     

基于LAN的农业科学研究院的科研仪器共享共用信息平台设计与实现

为提高科研院所大型仪器的利用率、降低科研成本,以浙江省农业科学院大型仪器信息化管理为研发对象,研发了大型仪器管理系统,重点论述了该系统的设计思想、系统结构及主要功能。     

猪的福利与放牧养猪

通过对动物福利的概况阐述,分析了我国猪饲养环境中存在的动物福利问题以及放牧养猪对猪只福利的影响,提出了发展放牧养猪的思路。     

猪C1QTNF3基因可变剪接体的鉴定及介导miR-101调控成脂分化的研究

旨在研究猪C1QTNF3基因可变剪接体的特性及miR-101通过C1QTNF3基因促进猪脂肪SV细胞成脂分化的机制。本研究采集3头30日龄健康马身猪仔公猪心、肝、脾、肺、肾、胃、股二头肌、腰大肌、皮下脂肪和背部脂肪组织样品,首先利用RT-PCR技术和生物信息学方法对C1QTNF3基因的不同转录本进行扩增和生物学特性分析,采用qRT-PCR技术检测C1QTNF3基因不同转录本在猪组织中的表达变化。随后,利用生物信息学预测发现,C1QTNF3上游的调控因子是miR-101,采用双荧光素酶报告试验验证miR-101对C1QTNF3的调控作用。最后,利用油红O染色和qRT-PCR技术检测过表达miR-101对猪脂肪SV细胞成脂分化的影响。基因克隆得到猪C1QTNF3存在两个转录本C1QTNF3和C1QTNF3-1,其中C1QTNF3-1为新鉴定的转录本;测序分析显示,与C1QTNF3相比,C1QTNF3-1的第1外显子区域缺失了219个碱基,少编码73个氨基酸。进化树分析显示,猪C1QTNF3和C1QTNF3-1蛋白序列与人和马来亚穿山甲等物种的亲缘关系较近。C1QTNF3和C1QTNF3-1在猪各组织中均有表达,且在各组织中C1QTNF3-1的表达量均极显著高于C1QTNF3（P<0.01）。过表达miR-101极显著下调C1QTNF3 3'UTR区域的荧光素酶活性（P<0.01）和C1QTNF3 mRNA的表达（P<0.01）,同时增加了脂肪细胞成脂分化关键基因PPARγ、C/EBPβ、SREBP-1c和FABP4的mRNA表达量（P<0.01）。本试验成功克隆了猪C1QTNF3基因的两个可变剪接体,其中C1QTNF3-1在猪不同组织中均高表达,推测该转录本为基因发挥功能的主要亚型。并深入研究了C1QTNF3基因的上游调控因子,揭示了miR-101靶向C1QTNF3促进成脂分化的机制,丰富了C1QTNF3的生物学作用和调控网络。     

中兽医药技术是猪只康养最佳供给体系

在我国养猪业面临产品安全和环境污染两大瓶颈的情势下,如何使所生产的猪肉食用卫生,所排粪污环境安全,纯天然、无污染、广效低毒、绿色环保的中兽医药学为破解这两大瓶颈提供了技术支撑。充分发挥我国传统的中兽医药技术的特色和优势,构建以中兽医药为主体的猪只康养技术供给体系,旨在集生产管理"适其天性"、所喂药料"无污染"、治疗药物"纯天然"、预防措施治"未病"、治疗方法"和谐调节"和四季保健"药饲同源"于一体的技术供给体系,从源头上破解这两大难题,从而实现猪只康养,环境安全,猪肉安康,凸显出中兽医药是猪只康养的最佳供给体系。     

超额储备后备母猪的运作模式——非洲猪瘟背景下集团化养猪企业的盈利之道

集团猪场代表行业最高技术、管理及现代化水平,迅速发展。特别是2018年非洲猪瘟疫情在我国暴发后,在高风险、高回报的行业背景下,集团化养猪企业凭借资金、人才、技术、硬件设施等优势,采取超额储备种猪的运作模式,进而迅速扩张。超额储备的本质是利用资金充足、场区数量、生物安全等优势,通过统一使用长白或大白公猪配种,育肥场用作种猪场等方式超额储备后备母猪。个别场区清场后可以迅速引种补栏,多余后备母猪、妊娠母猪正常出售回收资金。这种模式可有效降低风险、保证足够盈利,同时在非洲猪瘟疫情的背景下有效整合农业资源、利用商业资金反哺养猪业,指引养猪行业走向。     

生猪规模养殖场疫病防控面临挑战与对策

近年生猪养殖规模扩大的同时,很多疾病常并发感染混合感染,表现出复杂的临床症状,再加上免疫抑制性疾病普遍存在,给生猪养殖产业造成巨大影响。针对这种情况需要深入探讨规模化生猪养殖场疫病防控所面临的多种挑战,并从科学选址、完善防疫设施、构建综合性管理制度、加强生物安全屏障建设等多方面入手,及时化解疫病风险,将疫情控制在萌芽阶段,保证生猪养殖产业健康发展。     

小型猪近交系新品种的培育与开发利用

小型猪近交系是中国农业科学院北京畜牧兽医研究所以2头五指山猪(Sus scrofa)为系祖,历时15年,采用“近亲交配”、提高营养水平、同期发情以及笼架等饲养技术,逐步克服后代畸形率高、弱仔率高、成活率低等3大阶段性难题,育成国际上首个近交系猪.本文介绍小型猪近交系的培育过程、近交系研究鉴定、新遗传资源种质特性,以及开发利用.该研究成果填补了国内外近交系猪的研究空白,为大型哺乳近交系动物培育理论和应用研究提供了新的视角.     

Expression Analysis of THBS3 Gene in Different Tissues and Skeletal Muscles at Different Periods from Landrace and Tongcheng Pigs

To study the expression pattern of THBS3 gene in different tissues and during skeletal muscle development, the THBS3 gene expression in different tissues and skeletal muscles during prenatal periods (33, 45, 65, 70 and 90 d) and postnatal periods (0, 9, 30, 60, 120 and 160 d) from Landrace and Tongcheng pigs were detected by Real-time quantification PCR.The results showed that THBS3 gene widely expressed in all tissues examined, exhibiting similar spatial expression patterns with expression peaks in lung in both pig breeds except in stomach and intestine.Moreover, although THBS3 gene showed a significant higher expression level in gestation than after birth in Landrace and Tongcheng pigs (P<0.05), it exhibited different expression patterns between Landrace and Tongcheng pigs, the expression peak was detected at gestation day 45 in Landrace pig, while was detected at gestation day 65 in Tongcheng pig.The results suggested that THBS3 gene involved in skeletal muscle growth and development in pigs, as well as the regulation of asynchronization of skeletal muscle development in different pig breeds.