内蒙古贺兰山大型真菌资源现状及开发建议

文章根据2012年真菌科学考察采集到的标本,根据传统分类学方法,对内蒙古贺兰山真菌资源状况进行了鉴定.研究结果显示:研究区共有真菌262种,隶属于7目27科79属,真菌多样性丰富;其中食用菌占全部真菌种类的一半以上,药用菌和毒菌资源分别占24.81%和15.27%.最后对开发真菌资源给出了建议.     

Short-Term Effect of Nitrogen Intensification on Aggregate Size Distribution, Microbial Biomass and Enzyme Activities in a Semi-Arid Soil Under Different Crop Types

There is a lack of quantitative assessments available on the effect of agricultural intensification on soil aggregate distribution and microbial properties. Here, we investigated how short-term nitrogen(N) intensification induced changes in aggregate size distribution and microbial properties in a soil of a hot moist semi-arid region(Bangalore, India). We hypothesised that N intensification would increase the accumulation of macroaggregates 2 mm and soil microbial biomass and activity, and that the specific crop plant sowed would influence the level of this increase. In November 2016, surface(0–10 cm) and subsurface(10–20 cm) soil samples were taken from three N fertilisation treatments, low N(50 kg N ha~(-1)), medium N(75 and 100 kg N ha~(-1) for finger millet and maize, respectively),and high N(100 and 150 kg N ha~(-1) for finger millet and maize, respectively). Distribution of water-stable aggregate concentrations,carbon(C) and N dynamics within aggregate size class, and soil microbial biomass and activity were evaluated. The high-N treatment significantly increased the concentration of large macroaggregates in the subsurface soil of the maize crop treatment, presumably due to an increased C input from root growth. Different N fertilisation levels did not significantly affect C and N concentrations in different aggregate size classes or the bulk soil. High-N applications significantly increased dehydrogenase activity in both the surface soil and the subsurface soil and urease activity in the surface soil, likely because of increased accumulation of enzymes stabilised by soil colloids in dry soils. Dehydrogenase activity was significantly affected by the type of crop, but urease activity not. Overall, our results showed that high N application rates alter large macroaggregates and enzyme activities in surface and subsurface soils through an increased aboveground and corresponding belowground biomass input in the maize crop.     

Effect of a novel solution for organ preservation on equine large colon in an isolated pulsatile perfusion system

REASONS FOR PERFORMING STUDY: Several therapeutic agents have been tested in models of ischaemia and reperfusion injury (IRI) in equine jejunum, with mixed results. This study was based on the use of an organ perfusion solution (OPS) designed to protect human allografts from IRI. HYPOTHESIS: A modified OPS can preserve the integrity of equine large colon during 12 h of isolated pulsatile perfusion, in the absence of oxygen and blood. METHODS: Segments of large colon were removed from anaesthetised horses, the contents removed and the mucosa rinsed with 0.9% saline. Experimental segments were perfused for 12 h with one litre modified OPS (n = 7) delivered by pulsatile flow through an extracorporeal circuit. Control segments (n = 4) were perfused on the same circuit with one litre of autologous blood. Vascular resistance, flow and pressure were measured serially, and aliquots of OPS and blood drawn hourly for routine biochemical analyses. Mucosal biopsies of the experimental and control segments were taken at 0, 6 and 12 h and in vivo mucosal tissue at 0 h for baseline comparison. All biopsies underwent histomorphometric analysis and immunohistochemical assessment of calprotectin activity. RESULTS: All colon segments were machine perfused without technical complications. Vascular and biochemical indices remained constant over 12 h in the OPS group, and were constant over 6 h in the control group, but deteriorated later. Mucosal integrity, expression of cyclooxygenases-1 and -2, and expression of mucosal calprotectin were unchanged in the OPS group compared with the baseline tissues, and mucosal integrity was superior to the control tissues. CONCLUSIONS: A modified OPS designed to target specific pathways of damage from IRI can preserve colonic mucosal integrity for 12 h in the absence of blood and oxygen.     

大穗型籼稻不育系红广A的选育

红广Ａ是以珍汕９７Ａ为母本,用自选的红广为父本杂交,采用连续回交方法转育而成的大穗型籼稻不育系。其不育性稳定,柱头发达、外露率高,异交结实率高,易于繁殖制种;农艺性状好,配组杂种优势强。     

Determination of Fatty Acid Composition and Phospholipid Molecular Species of Large Yellow Croaker (Pseudosciaena crocea) Roe from China

The aim of this study is to investigate the composition of the fatty acids and phospholipids derived from large yellow croaker (Pseudosciaena crocea) roe. The composition of the total lipids and the molecular species of phospholipids were determined by gas chromatography–mass spectroscopy (GC–MS) and high performance liquid chromatography–evaporative light scattering detector (HPLC–ELSD), respectively. The results showed that large yellow croaker roe had high levels of the total lipid (19.6% ± 1.32%, w/w) and phospholipid (61.2% ± 1.22% of the total lipid). The phospholipid was rich in docosahexaenoic acid (31.0% ± 0.19% of the total phospholipids), and the major phospholipid molecular species was phosphatidylcholine (PC, 61.06% ± 0.02% of the total phospholipids, w/w). It was concluded that large yellow croaker roe is expected to be a good resource of phospholipids with a high content of PC.     

细水雾大空间局域环境调节系统的设计及应用

提出了大空间局域环境喷雾降温、增湿、调湿系统的设计方法.针对细水雾扩散蒸发过程,建立了大空间局域环境调节的数学模型.以上海世博会特钢大舞台喷雾降温系统为例,对系统终端的耗水量以及降温空间内的湿度变化进行计算分析后发现,在半室外环境喷雾降温过程中,由于环境温度的降低,空气的饱和含水量下降,水蒸气含量增加,局部环境湿度增大.采用人工送风对环境湿度进行调节,配风量对比计算表明,增大配风量可以加快环境湿度趋于稳定.设定降温终端的工作频率后,相对湿度可控制在88%以下.计算分析模型忽略了人体散热、散湿等非关键因素.所提出的系统设计、优化和分析方法已经成功应用于奥运会场馆工程,对世博轴及场馆等室外和半室外环境喷雾降温系统的设计也具有重要的参考价值.     

Effect of thermal radiation on thermal flow field in inclined tunnel

The thermal radiation is the main factor affecting internal thermal flow field in a tunnel. This paper studies on two open ends of a channel inclined with angles of 10°, 20° and 30° by experiments and numerical simulations. The experimental data agree well with the predicted results by using Large Eddy Simulation (LES) to solve N-S equations driven by the buoyancy flow. And the influence of thermal radiation on thermal flow field of tunnel with inclination angles from 10° to 30° at same heat source is obtained by comparing the experiment results and numerical resolutions of coupled with radiation model and without radiation model. Thermal radiation makes temperature near the upper wall of tunnel drop, and the influence of thermal radiation on bottom end of tunnel declines with the inclination angle increasing. However, the inclination angle has a little effect on temperature near the ceiling of the top end of tunnel. Simultaneously, the higher temperature zone leaning to the top end due to the thermal radiation is obtained, and the temperature at the bottom of the upper part of the tunnel increases. The findings of the study are important references for theory research of thermal flow field in inclined tunnels.     

法律法规修订背景下的规模化猪场引种实务

非洲猪瘟传入我国以来,单纯考虑疫情肆虐现实而漠视引种需求的官僚做法,和单纯考虑引种需求而漠视疫情肆虐现实的盲动做法,斗争颇为激烈之际,《中华人民共和国动物防疫法》《动物检疫管理办法》《中华人民共和国畜牧法》陆续修订,并分别自2021年5月1日、2022年12月1日、2023年3月1日起施行。在此背景下的规模化猪场引种该咋办？根据疫情防控经验和历史上的引种教训,笔者在技术推广过程中曾经形成了一套兼顾供需双方动物防疫要求、保障种猪引进质量的方法。现依照法律法规的修订进行了修改完善。修改完善后的规模化猪场引种实务要求科学制定引种方案、提前备妥隔离条件、引种团队现场核验给力、三管齐下保种猪个体质量、无损运输的措施全面到位、落地后依法报告和隔离。     

秋浦花鳜多元养殖模式关键技术

秋浦花鳜是安徽地方水产优良养殖品种。基于当前秋浦花鳜主要的养殖模式,对相关技术要点进行总结,系统归纳了秋浦花鳜池塘主养、混养,网箱养殖,以及大水面增养殖等多元养殖模式及其关键技术。在不同的养殖模式下,秋浦花鳜均表现出优异的适应性,经济价值及社会价值,可进一步示范应用。     

胞质型磷脂酶A2基因克隆及其表达量随大黄鱼仔稚鱼生长发育的变化

利用同源克隆技术和cDNA末端快速扩增(RACE)技术克隆大黄鱼胞质型磷脂酶A2(cPLA2)基因的cDNA全长.此外,应用实时定量PCR法检测不同日龄大黄鱼仔稚鱼cPLA2基因的表达量.序列分析结果表明:cPLA2基因全长2 550 bp(GenBank登录号:KF006240.1),其中5'端非编码区176 bp,开放阅读框2 169 bp,3'端非编码区205 bp,共编码723个氨基酸.系统进化树分析结果表明:克隆所得大黄鱼cPLA2基因与其他鱼类的cPLA2基因亲缘关系较近,与哺乳动物的亲缘关系较远.定量检测结果表明:大黄鱼仔稚鱼cPLA2基因的表达量随日龄的增加为先显著升高(1、3、7日龄vs.15日龄,P＜0.05),在15日龄时达到最高值,随后显著下降(15日龄vs.19日龄,P＜0.05),之后趋于平稳.由此可知,大黄鱼从仔稚鱼到幼鱼早期的变形过程中,磷脂分解代谢的关键酶cPLA2基因的表达量呈现有规律的变化,这可能对机体保持体内磷脂的动态平衡、维护细胞膜的流动性具有重要的意义.大黄鱼仔稚鱼cPLA2基因表达量的变化趋势在一定程度上可以衡量大黄鱼消化系统的发育情况.