ITS2序列作为青风藤DNA条形码的研究

采用DNA试剂盒法提取采自不同地区的青风藤及其类似植物的基因组DNA,设计通用ITS2引物进行PCR扩增和测序,运用生物信息学软件对序列进行分析,从而对青风藤及其类似植物进行分子鉴定。结果表明:产地为陕西、贵州、湖北的青风藤样品序列长度分别为380~437、429~436、434~438 bp,GC含量在54.2%~55.7%,平均GC含量约为55%。青风藤及其类似植物的ITS2序列存在较大差异,且平均K2P遗传距离明显大于青风藤的种内平均K2P遗传距离。这说明内转录间隔区2(ITS2)作为DNA条形码可准确地鉴别青风藤与其他类似植物,为中药材的鉴别提供了新途径。     

枣果病原真菌的分离鉴定及特性分析

对云南省蒙自市6个主要枣树栽培品种储藏期的腐烂果实表面的病原真菌进行分离和鉴定,得到25株优势病原真菌,分别扩增得到核糖体DNA转录间隔区(Internal transcribed spacer,ITS)序列,通过对ITS的生物信息学和系统发育分析,并结合菌落形态特征,鉴定病原真菌分别为链格孢属(Alternaria sp.)、曲霉属(Aspergillus sp.)、葡萄座腔菌属(Neofusicoccum sp.)和脉纹孢菌属(Neurospora sp.)。     

用于池塘养殖尾水处理的串联式旋流器水动力特性及分离效率

为了将多级旋流分离技术应用于池塘养殖尾水处理,并解决旋流器的水力停留时间短、分离效率低等问题,根据池塘养殖南美白对虾的尾水特点,结合工业标准150旋流分离器的结构参数,设计了一种串联式旋流分离器。该研究基于欧拉-拉格朗日方法,结合雷诺应力湍流模型与离散相模型,建立了串联式旋流分离器的三维两相内流场数值计算模型。在对比试验数据验证计算方法有效性和流场网格独立性的基础上,分析了进水口流量、锥角、颗粒粒径和密度等参数对旋流器的流场特征和分离效率的影响机制,并提出了多级“旋流+过滤”组合式池塘养殖尾水固液分离技术。研究表明：随着进水口流量在一定范围内增加,两级旋流器内流场的切向速度、轴向速度、分流比、压力降均增大,涡流强度增强,对于粒径为75μm颗粒物的分离效率明显提高,而对于粒径为5μm颗粒物的分离效率变化不显著;随着旋流器的锥角减小,水流阻力、压力降和分流比均降低,有助于增加颗粒物在旋流区的停留时间,从而提高其分离效率;随着颗粒物的密度和粒径增大,旋流器的分离效率提高,对粒径为75μm沉淀物的分离效率可达80%,对同粒径悬浮物的分离效率约为60%,但对于粒径为5μm的2类颗粒物而言,分离效...     

4个番荔枝品种比较试验

研究了4个番荔枝品种的植物学特性及果实的外观性状和内在品质性状,结果表明:AP番荔枝植株生长健壮,果皮表面平滑、鳞目连成一片,果肉风味香甜、微酸可口,果实维生素C、可溶性固形物、果糖、葡萄糖和蔗糖含量均较高,且种子少、可食率高,是一个值得大面积推广的优良番荔枝品种.     

沙湖水体富营养化评价及防治对策

本文采用营养状态指数法对沙湖营养状况进行综合评价。评价结果表明,沙湖富营养化日趋严重,已经由2002年的轻度富营养发展到2005年的中度富营养,磷和有机污染物是沙湖富营养化限制因子。对富营养化成因进行分析,结果表明外源污染和内源释放是造成沙湖富营养化的主要原因,黄河补水和地表径流是主要的外污染源,最后根据分析结果提出沙湖富营养化的防治对策。     

血液透析患者动静脉内瘘血栓形成的治疗方法探讨

目的总结血液透析患者动静脉内瘘后血栓形成的治疗经验。方法 156例动静脉内瘘后血栓形成患者,早期采用溶栓治疗,而晚期采用手术治疗。结果 47例采用溶栓治疗,成功率为53.2%,其中6h内、6h后溶栓成功率分别为85.7%、26.9%(P<0.01)。131例(含溶栓失败22例)手术治疗者中,成功率为84.7%,其中静脉-动脉端侧吻合、取栓加吻合口扩张的成功率分别为94.2%、48.1%(P<0.01)。结论动静脉内瘘后血栓形成患者在阻塞6h内采用溶栓治疗,而阻塞超过72h者首选静脉-动脉端侧吻合手术。     

多目标随机结盟对策的区间模糊ZS -值

内部控制和企业价值之间存在着密切的联系，内部控制是否有效及效率高低影响着企业的价值。内部控制质量高的企业有动机通过自愿性信息披露向市场传递企业价值较高的信号，由于信号传递作用，企业更容易获得较低成本的商业信用和银行信用借款，提升了企业价值，而内部控制质量低的企业没有动机进行内部控制信息披露，从而不得不付出较高的债务融资成本，降低了企业价值。     

小微企业内部会计控制问题与对策

在经济全球化的影响下，小微企业已成为我国社会经济的主力军，在各个行业都得到了不同程度的发展。同时，内部会计控制已经成为企业内部控制的子系统，在企业中占据着重要的角色，有利于推动企业的经营管理，实现长远发展。文章分析我国目前小微企业内部会计控制存在的问题，在此基础上对存在的问题进行归因分析，并提出了相应的解决办法。     

Application of a putative fatty-acid binding protein to discriminate serologically the two European quarantine root-knot nematodes, Meloidogyne chitwoodi and M. fallax, from other Meloidogyne species

Two major proteins, Mcf-A67 and Mcf-B66, were identified by mini two-dimensional polyacrylamide gel electrophoresis in order to distinguish the two European quarantine root-knot nematodes, Meloidogyne chitwoodi and M. fallax, from eight other species. These quarantine proteinic markers have been microsequenced after enzymatic digestion. The internal amino acid sequences exhibit similarities to members of a family of low molecular weight intracellular lipid-binding proteins. Moreover, to explore a simple, rapid, and inexpensive way to identify the two quarantine nematodes, dot blot hybridizations were performed using an antiserum (A67) produced from the longest amino-acid sequence of the protein Mcf-A67. Although several proteins stained on the M. chitwoodi and M. fallax western blot membranes, the two nematodes were easily distinguished from other root-knot nematodes, on dot blot assays with soluble proteins extracted from a single female. Because of its specificity and sensitivity, the use of the A67 antiserum to improve the diagnosis of the two European quarantine root-knot nematodes is discussed.     

Ribosomal DNA Sequence Comparisons of Colletotrichum Graminicola from Turfgrasses and other Hosts

The 5.8S ribosomal gene and the flanking internal transcribed spacers (ITS) 1 and 2 from Colletotrichum graminicola isolates causing anthracnose disease of Agrostis palustris and Poa species were sequenced. Although bootstrap support was not high, two major groups were observed with both UPGMA and parsimony algorithms, one containing isolates from A. palustris and another with isolates from Poa spp. The ITS sequences were also compared with those of isolates of C. graminicola and C. sublineolum from Sorghum spp., Zea mays and Rottboellia cochinchinesis as well as other Colletotrichum species. Except for one isolate from P. annua in Texas, the ITS1 and ITS2 sequences of turfgrass isolates always grouped separately from C. graminicola or C. sublineolum from non-turfgrass hosts with high bootstrap support. ITS sequences of the turfgrass isolates were more similar to those of other species of Colletotrichum, such as C. coccodes and C. dematium, than they were to C. graminicola isolates from other hosts. Turfgrass isolates have ITS sequences which are not identical to those of isolates from Zea mays and Sorghum species demonstrating diversity among fungi conventionally classified as C. graminicola.