Long‐term survival of Acidovorax citrulli in citron melon (Citrullus lanatus var. citroides) seeds

Long‐term survival of Acidovorax citrulli in citron melon (Citrullus lanatus var. citroides) seeds was investigated. Citron melon seed lots infected with A. citrulli were generated in the field by inoculating either the pistils (stigma) or pericarps (ovary wall) of the female blossoms. Seventeen A. citrulli isolates from 14 different haplotypes belonging to two different groups (group I and II) were used for inoculation. After confirming that 100% of seed lots were infected, they were stored at 4°C and 50% RH for 7 years. After storage, the viability of A. citrulli cells from individual lots was determined by plating macerated seeds on semiselective medium as well as growing seeds for 14 days and scoring for bacterial fruit blotch symptoms. The type of A. citrulli isolate (group I or group II) used did not significantly influence bacterial survival. However, A. citrulli survival was significantly greater in seed lots generated via pistil inoculation (52·9 and 29·4%) than via pericarp inoculation (23·5 and 17·6%). Repetitive extragenic palindrome (rep)‐PCR on A. citrulli isolated from citron melon seed lots after storage displayed similar fingerprinting patterns to those of the reference strains originally used for blossom inoculation, indicating that cross‐contamination did not occur. The results indicate that A. citrulli may survive/overwinter in citron melon seeds for at least 7 years and bacterial survival in seed was influenced more by method of blossom inoculation than by the type of bacterial isolate.     

国内外果蔬生物保鲜方法的研究现状与展望

概述果蔬生物保鲜方法的种类、原理和技术,同时对现阶段生物保鲜技术在果蔬贮藏保鲜中的应用进行综述,最后对生物保鲜技术存在的问题和未来研究方向进行展望.     

几种果树当年实生苗生长发育规律的研究

研究了山杏、毛樱桃、黄海棠、山梨4种果树当年实生苗的生长发育规律。结果表明,根系对苗木的生长有很大影响,尤其侧根的生长情况至关重要。7月份是此4种苗木在哈尔滨地区生长的最关键时期。地上部与地下部的生长具有既对立又统一的关系。     

果蔬采后高O2处理研究进展

综述了高O2处理对果蔬采后呼吸强度、乙烯释放量、色泽、品质、褐变及病原微生物的影响,分析了高O2处理在果蔬保鲜中的可行性与应用前景.     

桑椹与叶片蛋白质双向电泳样品制备方法的比较试验

为建立适合于桑树蛋白质组研究的双向电泳技术体系,以桑品种"大10"的桑椹与叶片为材料,探讨了桑树蛋白质不同提取方法对凝胶电泳效果的影响。结果表明,改良酚提取法与常规的SDS提取法、尿素/硫脲提取法相比,电泳图谱清晰,分离效果良好,能检测到更多的蛋白质组分,适用于桑椹与叶片蛋白质IPG-IEF/SDS-PAGE双向电泳及SDS-PAGE电泳的样品制备。     

农艺农机融合的设施番茄栽培参数优化研究

为了提高设施番茄栽培关键环节机械化水平,从农艺农机融合角度出发,在保证番茄产量和品质的基础上,亟需研究提出宜机械化操作的农艺标准.本试验以设施番茄为研究对象,以现有栽培密度和栽培畦规格为对照,通过春、秋两茬栽培试验,探究了设施番茄宜机化栽培的栽培畦参数及适宜密度.结果表明:在每667 m2栽培株数不变的条件下,改1.4...     

联合气调包装贮藏对樱桃保鲜效果的影响

采用冷藏条件下联合自发气调包装(CMP)方式贮藏樱桃进行了调查研究,结果表明,贮藏10天樱桃的呼吸强度为4.1 mgCO2/kg·h左右,腐烂率基本控制在10%以内.与纯冷藏相比,联合气调包装保鲜方式可以有效地降低采后樱桃的呼吸强度与腐烂率,有效地保持了采后樱桃的硬度,提高其耐贮性,经济效益显著.     

果蔬热物性的研究与比较

利用测量热物性的实验装置与计算模型,对多种果蔬进行实验数据的研究比较,找出较为准确的方法,通过研究发现式(2)(6)和式(17)较好,并为今后果蔬热物性的研究提供理论基础.