木荷1代育种群体遗传多样性分析

木荷为我国亚热带地区主要的珍贵优质阔叶用材树种和生态防护树种.利用筛选的14对多态性强的SSR引物,对木荷1代育种群体中来自15个产地133个亲本进行遗传多样性分析,为其优异种质资源保存、杂交亲本选配及新种质创制提供科学依据.结果表明:14对引物共扩增86个位点,每对引物检测到的等位基因数(Na)变异范围为2～11个,平均等位基因数(Na)为6.14个,平均有效等位基因数(Ne)为3.23个,平均观察杂合度(Ho)为0.572 0,平均Shannon信息指数(I)和平均Nei's基因多样性指数(Nei)分别为1.224 7和0.599 0,说明木荷1代育种群体具有丰富的遗传多样性,其中,福建建瓯产地遗传多样性最高,浙江遂昌产地遗传多样性最低.木荷1代育种群体中成对亲本间遗传距离为0.023 3～1.633 8,平均为0.6067.不同产地遗传多样性与纬度呈显著的负相关关系(r=-0.5162,p=0.048 9).通过UPGMA聚类,可将133个育种亲本分成3个类群,其中,类群3又分为4个亚类群.木荷亲本选配时,应充分考虑优树的产地来源.     

大豆SSR指纹图谱身份证的研究进展与展望

随着新育成大豆品种数量的不断增加以及分子生物学技术的发展,指纹图谱身份证的构建逐渐成为当今品种鉴定的重要技术手段。文中综述了SSR指纹图谱身份证技术在大豆品种鉴定方面的应用进展;综合分析了当前SSR指纹图谱身份证构建技术存在的问题,并提出相应的改进方向,以期为科学快速准确地鉴定大豆品种,评价种质资源和解决品种产权纠纷提供参考依据。     

从保守主义的角色演变看中国近代文化的发展进路

在中国近代文化转型的过程中,保守主义在各个时期都充当了传统守护者的角色,只是随着历史的发展,它所扮演的角色也不断有所变换。这是因为他们所要保守和所能保守的东西变得越来越少,同时在精神上却又越来越被放大,有时甚至显得很玄妙。在洋务运动时期,保守主义者几乎排斥一切西方文化,要保守住整个的中国传统不变。到了维新变法时期,"师夷长技"已不成问题,保守主义者乃要求师夷当止于工艺,中国传统政教制度不可变。到了排满革命高潮时期,否定传统政教制度又几乎不成问题,保守主义者乃呼吁要珍惜和保存"国粹",而"国粹"到底是什么东     

Looks can deceive: Molecular identity of an intraerythrocytic apicomplexan parasite in Australian gliders

Two yellow-bellied gliders (Petaurus australis) had an intraerythrocytic parasite closely related to the cyst-forming coccidia (Apicomplexa: Sarcocystidae). The parasitaemia persisted for 3 months or more but was observed to clear within 3 years in captivity. The parasite appears not to significantly debilitate its infected host. Traditionally, using morphological identification, the intraerythrocytic parasite would have been classified within the Hepatozoon species typically found in red blood cells. However, molecular diagnostic techniques targeting the parasite's SSU rDNA and LSU rDNA demonstrated the unusual identity of this blood parasite and disputed its identity as a haemogregarine parasite of the genus Hepatozoon. The sequence was compared with available sequences from diverse mammalian and non-mammalian blood parasites (malaria, piroplasms, hemosporidia and sarcosporidia). The intraerythrocytic blood parasite was found to be most closely related to the cyst-forming coccidia including Besnoitia spp., Cystoisospora spp., Hammondia spp., Hyaloklossia lieberkuehni, Neospora caninum, Sarcocystis spp. and Toxoplasma gondii. The life cycle of this intraerythrocytic parasite remains unknown. The presented DNA identification demonstrates its suitability for an improved identification of blood parasites.     

守望、妥协还是遗忘？——从白先勇的《安乐乡的一日》探析身份认同

短篇小说《安乐乡的一日》通过一个华人家庭在美国的生活表现了华人所负载的中国文化与美国文化之间的冲突和斗争。在美国的主流文化中,脱离中华母体文化的华裔已经被边缘化为“他者”。他们既无法得到移民国家（本文指美国）的认同,又由于时空的疏离而与祖国有着隔膜的尴尬状态。因而,对于身份的焦虑、对于文化身份的追问与认同渐渐地成为海外华文文学描写和深层挖掘的主题。笔者从小说中的人物形象分析入手来探析华裔的身份认同问题。     

差异与同一：生态世界观的两个基点

人与自然既有同一性又有差异性,因而人与自然的同一性与差异性构成了生态世界观的两个基点。人与自然同一性是二者和谐共处的基础,人与自然差异性是确立人之主体地位的基础。人与自然的相互规定性是生态世界观的前提条件,自然选择与社会选择的相互规定性是生态世界观的现实基础。     

美国华裔文学中女性身份的建构——以《女勇士》和《喜福会》为例

美国华裔文学发展至今已有数十年历史,其诞生和成长同美国社会息息相关。自上世 纪八十年代开始,在妇女解放运动深入的推动作用下,美国华裔文学中的女性文学异军突起并 受到了广泛关注。随着多元文化共同发展趋势的不断深入,越来越多的专家学者们开始关注美 国华裔文学的研究,在这一背景下,本文以《女勇士》以及《喜福会》两部文学作品为例,对美国华 裔女性文学进行简要探讨,解读其中的女性形象,希望能够为女性文学的进步、东西方文化冲突 的消弭以及多重文化视角写作的发展等提供些许参考。     

Identification and pathogenicity to rainbow trout, Oncorhynchus mykiss (Walbaum), of some aeromonads

Twelve strains of fish pathogenic aeromonads were identified by 16S rRNA sequencing as Aeromonas bestiarum , A. hydrophila , A. hydrophila subsp. dhakensis , A. salmonicida subsp. salmonicida , A. sobria biovar sobria and A. veronii biovar sobria. Following intramuscular injection, A. hydrophila subsp. dhakensis caused dark liquefying, raised furuncle-like lesions in rainbow trout within 48 h. Extracellular products of all cultures contained gelatinase and lecithinase, and most revealed lipase. Congo red absorption and siderophore production was recorded, but not so the suicide phenomenon or slime production. Sodium dodecyl sulphate polyacrylamide gel electrophoresis profile of the outer membrane proteins (OMP) revealed 10–25 bands, of which major bands were seen in the region of 32.5–47.5 and 62–83 kDa. Marked heterogenicity of the OMP and whole cell protein (WCP) profiles within and among the species was observed. Polypeptides of 83–173 kDa were detected in the WCP profile of the cultures, but they were not expressed in OMP fractions.     

新疆红胫戟纹蝗痘病毒包涵体蛋白基因序列分析

通过对红胫戟纹蝗痘病毒Dociostarus kraussi EPV(DkEPV)包涵体蛋白基因克隆、测序与同源性分析,结果显示,DkEPV包涵体蛋白基因包含2 922 bps的开放阅读框架, 编码109.4 kDa蛋白质.与鳞翅目及鞘翅目昆虫痘病毒包涵体蛋白氨基酸的同源性小于20;,而与其他直翅目昆虫痘病毒包涵体蛋白氨基酸的同源性均高于80;.DkEPV包涵体蛋白包含19个半胱氨酸位点,主要分布在C-末端.此包涵体蛋白基因启动子区域基因,富含A+T,并且具有典型的痘病毒晚期启动子信号TAAATG,直翅目昆虫痘病毒之间此序列保守.同时在此痘病毒包涵体基因的下游克隆了另一个不完整的基因序列,与血黑蝗痘病毒的MSV072基因同源,与包涵体蛋白基因比邻但方向相反.     

Effectiveness of SSR molecular markers in evaluating the phylogenetic relationships among eight Actinidia species

The aim of the present study is to evaluate a set of microsatellites and their effectiveness in determining the phylogenetic relationships among Actinidia species. A set of 14 genotypes belonging to eight Actinidia species were subjected to PCR using SSRs as molecular markers. The PCR products were analyzed on denaturing polyacrylamide gels. The discriminating ability of SSRs was based on the number of alleles and the indices DI, I and PIC. All SSR primer pairs were polymorphic and identified a total number of 61 alleles corresponding to an average of 7.8 alleles per locus. Data showed that the di-nucleotide microsatellites were more polymorphic, than the tri-nucleotide and penta-nucleotide, and were more efficient in establishing genetic similarities. The genetic similarity of the genotypes calculated with Jaccard and/or Dice similarity coefficients varied from 0.100 to 0.579, indicating a broad genetic base for the genetic material tested. Both similarity matrices clustered either with UPGMA or NJ, produced similar topology with minor clustering differences among genotypes. Thus, the eight species were clustered in two main groups and each main group in two subgroups. Data showed a close genetic relationship among Actinidia chinensis and Actinidia deliciosa species. The same conclusions were, also, drawn using the principal coordinate analysis.